구리 결합 펩타이드의 발현에 의한 대장균 균체의 구리 함량 증가

김형기,문성현,김우연,Kim, Hyung-Kee,Moon, Sung-Hyun,Kim, Woo-Yeon 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.1

Cloning and expression of copper-binding peptide gene in E. coli was carried out to enhance the copper-chelation capacity. E. coli was transformed with pET vector containing the copper-binding region of potato polyphenol oxidase gene and polyhistidine-coding DNA, and the copper content of E. coli harboring each vector was measured. No increase in intracellular copper was observed in E. coli harboring PPOCBpET32 vector, which contains DNA for polyphenol oxidase copper-binding region. Intracellular copper content of E. coli harboring pE728a vector, which contains one hexahistidine unit DNA, was 2,500 ppm after culturing without kanamycin, whereas E. coli harboring pET-his vector, which contains nine hexahistidine unit DNAs was 3,200 ppm. 감자 polyphenol oxidase의 구리결합지역 DNA와 histidine 다량 함유 인공 펩타이드를 암호화하는 DNA를 대장균 벡터에 각각 클로닝하여 발현시킨 후 대장균 내의 구리함량 증감을 조사하였다. Polyphenol oxidase의 구리결합지역 DNA를 포함하는 PPOCBpET32 벡터를 함유하는 균주의 경우는 벡터를 함유하지 않는 대장균 대조구보다 오히려 구리 함량이 약간 감소하여 약 600ppm의 간을 보여주어, 감자 polyphenol oxidase 구리결합지역의 대장균 내에서의 발현이 구리 함량 증가에 기여하지 못함을 알 수 있었다. 반면에 한 개의 hexahistidine 단위 DNA를 포함하는 pET28a 벡터 함유 대장균 균주를 knamycin 미첨가 배지에서 배양한 경우에는 구리 함량이 약 2,500ppm으로 높게 나타났다. 한편 hexahistidine 9개로 구성된 polyhistidine을 암호화하는 DNA를 포함하는 pET-his 벡터 함유 균주를 kanamycin 미첨가 배지에서 배양한 경우에 구리함량이 약 3,200ppm으로 나타나, 하나의 hexahistidine 단위만 발현하는 균주와 비교하여 구리함량이 약 30% 증가됨을 알 수 있었다.

이주배경 학생의 증가에 따른 교사 다양화 방안 탐색: 미국 사례를 중심으로

김형기(Hyung Kee Kim),이은채(Eun Chae Lee) 대구대학교 다문화사회정책연구소 2023 현대사회와 다문화 Vol.13 No.1

지속가능한 인간발전을 위한 복지체제

김형기 ( Hyung Kee Kim ) 한국응용경제학회 2012 응용경제 Vol.14 No.2

이 글은 복지를 “자유로운 인간발전에 대한 사회보장”으로 정의하고 지속가능한 인간발전을 위한 복지체제 구축의 방향을 제시하고자 한다. 노동연계복지와 보편적 복지가 복지공동체라는 새로운 복지 패러다임 속에 융합되고, 노동시장의 유연안전성이 실현되며, 교육-고용-복지의 연계가 이루어질 때, 지속가능한 인간발전을 위한 복지체제가 구축될 수 있다. 복지공동체가 실현되기 위해서는 복지정책의 지방분권이 실현되고 지역파트너십이 형성되어야 한다. 노동시장 유연안전성 삼각형 분석을 통해 보면, 한국에서 지속가능한 인간발전을 위한 복지체제 구축을 위해서는 실업급여 소득대체율을 크게 높이고 적극적 노동시장 정책 지출 비율을 대폭 증대시켜야 함을 알 수 있다. 이러한 새로운 복지패러다임이 구현되기 위해서는 ‘복지공동체 연합’과 노동시장의 유연안전성 실현을 위한 계층연합인 ‘유연안전성 연합’을 실현하는 방향으로 사회적 합의가 이루어져야 한다. This paper argues that a welfare regime for sustainable human development can be established when workfare and universal welfare merge into the welfare community which is a new welfare paradigm; when flexicurity of labor market is realized; and when a nexus "education-employment-welfare" is formed. A comparative analysis of "flexicurity triangles" in some major OECD countries suggests that Korea needs to increase greatly unemployment replacement rates and public expenditure for active labor market policy in order to build a welfare regime for a sustainable human development. It will be argued that "welfare community coalition" and "flexicurity coalition" are necessary for the new welfare paradigm.

복부대동맥류 수술 후 장기 추적관찰 결과

김형기(Hyung-Kee Kim),조민정(Min-Jung Jo),최향희(Hyang Hee Choi),허승(Seung Huh),김영욱(Young-Wook Kim) 대한외과학회 2008 Annals of Surgical Treatment and Research(ASRT) Vol.74 No.1

Purpose: The purpose of this study was to analyze the causes of late death and the long-term survival of patients following abdominal aortic aneurysm (AAA) repair. Methods: From 1993 to 2005, 146 (98 intact and 48 ruptured) consecutive AAA patients underwent open repair. The perioperative data was supplemented with a retrospective review of the medical records and the use of the database of the National Statistical Office to calculate the survival rates and the causes of death. Results: There was one operative death (1%) in the intact AAA group and 12 (25%) in the ruptured AAA group. After exclusion of the operative deaths, the survival rates of the intact AAA patients were 94.5% at 1 year, 77.0% at 5 years and 33.2% at 10 years; these findings demonstrated a significant longer survival for the intact AAA patients than for patients with ruptured AAA (86.1% at 1 year, 56.7% at 5 years and 0% at 10 years, respectively). The causes of late death included cardiovascular disease in 52.8% (coronary artery disease in 22.6%, cerebrovascular disease in 20.8%, other arterial aneurysms in 5.7% and graft-related death in 3.8%), malignancies in 11.3% and pulmonary disease in 11.3%. The multivariate analysis showed that the long-term survival rates were influenced by the rupture status, the history of cerebrovascular disease and hypertension. Conclusion: This study showed that the long-term survival after intact AAA repair was better than that for ruptured AAA repair even if we exclude the operative deaths. The most common cause of late death was cardiovascular disease. Our findings suggest that the patients with preoperative cerebrovascular disease and hypertension require additional care.

복부대동맥류 환자에서 합병된 원발성 대동정맥루

김형기(Hyung-Kee Kim),최향희(Hyang Hee Choi),허승(Seung Huh) 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.76 No.1

Primary aortocaval fistula (ACF) is a rare complication of abdominal aortic aneurysm (AAA), occurring in only 4% of ruptured cases and less than 1% of all AAAs. The hemodynamic changes resulting from the major shunt caused by the ACF include a sudden increase in inferior vena caval pressure and volume with renal venous hypertension and a decrease in total peripheral resistance. So, if untreated, it leads to irreversible high output heart failure. Preoperative diagnosis is crucial, as adequate preparation should be made for massive bleeding expected at operation. Successful treatment depends on management of perioperative hemodynamics, control of bleeding from fistula and prevention of thromboembolism. We report two cases of successfully treated spontaneous ACF with a review of the literature.

1980년대 한국자본주의 : 구조전환의 10년

김형기 ( Hyung Kee Kim ) 한국경제학회 1996 經濟學硏究 Vol.44 No.4

흰쥐의 허혈-재관류로 손상된 간에서 Alpha-Tocopherol의 효과

유운용(Wun Young Yu),김형기(Hyung Kee Kim),권준택(Jun Tack Kwon) 대한소화기학회 1995 대한소화기학회지 Vol.27 No.3

Background/Aims: Ischemia-reperfusion injury is responsible for organ damage in a variety of pathological events such as myocardial infarction, stroke and graft failure after organ transplan- tation. Reperfusion is essential for the salvage of ischemic state. But reperfusion itself is also thought to be accompanied by its own component of injury. Reperfusion is a particularly potent stimulus for free radical formation. Free radical attack on biological membranes, such as the plasma membrane, mitochondria and endoplasmic reticulum, can lead to the oxidative destruction of the polyunsaturated fatty acids of the membranes through lipid peroxidation. In the present study, the possible protective effect of alpha-tocopherol against 15 min ischemia and subsequent 60 min reperfusion in the rat liver was investigated. Methods: Ischemia of the left and median lobes was induced by ligation of the left portal vein and the hepatic artery for 1S min. 60 min after reperfusion, experiments were initiated. Results: In ischemia-reperfusion on liver, serum AST and ALT activities and the level of malondialdehyde(MDA) in the liver homogenate were significantly increased. In addition, levels of cytochrome P-450 decreased in a microsomal fraction during ischemia-reperfusion. When rats were pretreated with alpha-tocopherol(200 mg/kg p.o, daily for 3 days), alpha-tocopherol suppressed the elevation of serum AST, ALT and MDA level and the decrease in cytochrome P-450 content. Conclusions: Free radicals play a major role in ischemia- reperfusion injury in the liver. Alpha-tocopherol exerts its beneficial effect on liver ischemia-reperfusion injury. Therefore, anti-oxidative effect of alpha-tocopherol is considered a probable suppressing mechanism on liver injury caused by ischemia-reperfusion. (Korean J Gastroenterol 1995;27:323 - 330)

Prevention of Recurrent FSGS with Cyclosporine and Plasmapheresis Prior to Renal Transplantation

양은애,박효민,조민현,고철우,김형기,허승,Yang, Eun-Ae,Park, Hyo-Min,Cho, Min-Hyun,Ko, Cheol-Woo,Kim, Hyung-Kee,Huh, Seung Korean Society of Pediatric Nephrology 2010 Childhood kidney diseases Vol.14 No.1

국소 분절성 사구체 경화증은 소아 말기 신부전의 중요한 원인이며, 신이식 후에도 재발되는 특징을 보이는데, 이러한 재발에 대한 치료로는 혈장교환술과 사이클로스포린과 같은 면역억제제의 투여 등이 있다. 이에 저자들은 신이식 전 혈장교환술과 예방적 사이클로스포린을 투여하여 국소 분절성 사구체 경화증의 재발을 효과적으로 예방한 경험 2례를 보고하는 바이다. We report on two children with a high risk of recurrent focal segmental glomerulosclerosis (FSGS) after renal transplantation that could be effectively prevented by prophylactic administration of cyclosporine combined with preemptive plasmapheresis prior to renal transplantation.

하지정맥류 환자 및 정상인에서 대복재정맥의 기질 금속단백분해효소 및 금속단백분해효소-조직억제제의 발현도 분석

허승(Seung Huh),최향희(Hyang Hee Choi),김형기(Hyung-kee Kim),김상준(Sang Joon Kim) 대한외과학회 2010 Annals of Surgical Treatment and Research(ASRT) Vol.79 No.6

Purpose: Although varicose veins are very common in adults, the mechanism of the disease has not been established. Degradation of the extracellular matrix is regulated by various matrix metallopreteinases (MMPs) and their inhibitors tissue inhibitor of metallaproteinase (TIMPs). This study was performed to analyse the relationship between venous wall degeneration and expression of these matrix proteinases. Methods: Twelve great saphenous vein (GSV) segments from 7 patients without varicose veins (control) and 86 GSV segments from 18 patients (22 limbs) with varicose veins (C2,4,5EPASPR) were used for this study. Light microscopic examination was used in the evaluation of vein wall degeneration, immunohistochemistry and Western blotting for the expression of MMPs (MMP-1, MMP-2, MMP-9 and MMP-13) and TIMPs (TIMP-1 and TIMP-2), and zymography for gelatinolytic activity of MMP-2 and MMP-9 were performed. Results: MMP-9 was more strongly expressed in the vein wall of both control and patient groups, especially in the endothelial cells and medial muscle layers and TIMP-2 followed. The expression of MMP-9 was closely related to the degree of venous wall degeneration. Activated MMP-2 and MMP-9 were observed in both groups and expressed more in the proximal GSV of the patients. In the Western blotting, the expression of MMP-9 and TIMP-1 were significantly higher than other MMPs and TIMP-2 in the patients with varicose veins. Conclusion: MMP-9 is much more expressed in the wall of degenerative veins. This matrix-degrading enzyme may play an important role in the degeneration of venous wall followed by its remodeling.

외상 후 지연성으로 발생한 장간막 동정맥루: 증례보고

조자윤 ( Jayun Cho ),정희경 ( Hee Kyung Jung ),김형기 ( Hyung Kee Kim ),임경훈 ( Kyoung Hoon Lim ),천재민 ( Jae Min Chun ),허승 ( Seung Huh ),박진영 ( Jin Young Park ) 대한외상학회 2013 大韓外傷學會誌 Vol.26 No.3

Introduction: A post-traumatic mesenteric arteriovenous fistula (AVF) is extremely rare. Case Report: A previously healthy 26-year-old male was injured with an abdominal stab wound. Computed tomography (CT) showed liver injury, pancreas injury and a retropancreatic hematoma. We performed the hemostasis of the bleeding due to the liver injury, a distal pancreatectomy with splenectomy and evacuation of the retropancreatic hematoma. On the 5th postoperative day, an abdominal bruit and thrill was detected. CT and angiography showed an AVF between the superior mesenteric artery (SMA) and the inferior mesenteric vein with early enhancement of the portal vein (PV). The point of the AVF was about 4 cm from the SMA`s orifice. After an emergent laparotomy and inframesocolic approach, the isolation of the SMA was performed by dissection and ligation of adjacent mesenteric tissues which was about 6 cm length from the nearby SMA orifice, preserving the major side branches of the SMA, because the exact point of the AVF could not be identified despite the shunt flow in the PV being audible during an intraoperative hand-held Doppler-shift measurement. After that, the shunt flow could not be detected by using an intraoperative hand-held Doppler-shift measuring device. CT two and a half months later showed no AVF. There were no major complications during a 19-month followup period. Conclusion: Early management of a post-traumatic mesenteric AVF is essential to avoid complications such as hemorrhage, congestive heart failure and portal hypertension.