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Agrobacterium tumefaciens 공동배양법을 이용한 옥수수 형질전환체 생산
조미애,박윤옥,김진석,박기진,민황기,유장렬,최필선,Cho Mi-Ae,Park Yun-Ok,Kim Jin-Suck,Park Ki-Jin,Min Hwang-Ki,Liu Jang-Ryol,Clemente Tom,Choi Pil-Son 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.2
옥수수 미숙배배양과 Agrobacterium tumefaciens공동배양법에 의해 형질전환체를 생산하였다. Hi II계통의 미숙배를 Ubiquitin 1 promoter-GUS유전자와 선발마커로서 nptII 유전자로 제작된 pPTN290벡터를 C58C1에 도입한 후 형질전환 균주로 사용하였다. 7개의 paromomycin저항성 배 발생캘러스를 얻었으며, GUS양성반응을 나타내는 7개의 독립적인 식물체를 얻었다. Southern분석법에 의하여 $T_1$세대 식물체로부터 nptII유전자가 안정적으로 도입되어 있음을 확인하였다. Agrobacterium tumefaciens-mediated immature embryo transformation was used to produce transgenic maize. Immature embryo of Hi II genotype were co-cultivated with strains Agrobacterium tumefaciens (C58C1) containing the binary vectors (pPTN290) carrying with Ubiquitin promoter-GUS gene as reporter gene and NOS promoter-nptll gene conferring resistance to paromomycin as selective agent. Seven embryogenic callus lines transformed showed the resistance in paromomycin antibiotics. Histochemical GUS assay showed that 7 individual lines transformed with the GUS gene were positive response among the transformants. Southern blot analysis revealed that the nptll gene segregated and expressed in their progeny.
국내 옥수수 품종 및 계통의 미숙배 배양으로부터 Yellowish Friable Embryogenic 캘러스 (YFEC) 생산과 식물체 재생
조미애,박윤옥,김진석,박기진,민황기,유장렬,최필선,Cho Mi-Ae,Park Yun-Ok,Kim Jin-Suck,Park Ki-Jin,Min Hwang-Ki,Liu Jang-Ryol,Choi Pil-Son 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.2
국내 옥수수 3품종 (두메찰, 미백찰, 흑점찰)과 5 계통(HW1, KL103, HW3, HW4, KW7)의 미숙배를 1 mg/L 2,4-D, 25 mM proline, 100 mg/L casamino acid, 3 mM MES, 1.7 mg/L $AgNO_3$, Eriksson's vitamin 및 20 g/L sucrose가 첨가된 MS배지 (SEM)에 5주 동안 배양하였다. 각 계통 및 품종의 체세포배 발생 빈도는 HW1 (45.20%), KL103 (5.75%), HW3 (37.20%), HW4 (30.10%), KW70 (55.20%), 미백찰 (18.74%), 흑점찰 (22.41%), 두메찰 (36.72%)이었으며, 모델 품종인 Hi II type에서는 10% 이하였다. Hi II계통에서 형성되는 type II캘러스와 같은 노란색의 부드러운 배발생캘러스 (yellowish friable embryogenic callus, YFEC)는 오직 HW3와 흑점찰 품종에서 형성되었으며, 반면 다른 품종 및 계통에서는 late-embryo단계의 단단한 체세포배가 직접 발생되거나 형태적으로 비정상 모양을 갖는 단단하고 팽창된 체세포배가 발생 되었다. 노란색의 부드러운 배발생캘러스 (yellowish friable embryogenic callus, YFEC)는 동일배지에서 빠르게 증식 되었고 6개월 이상 배발생 능이 유지 되었으며, 1차 재분화 배지와 2차 재분화배지에 옮겼을 때 모두 식물체로 전환 되었다. 그러나 단단한 노란색의 배발생캘러스와 비정상 형태의 체세포배는 동일배지에서 매우 느리게 증식되었고, 재분화 배지에서 낮은 식물체 전환율 (25%)을 보였다. 따라서 본 연구로부터 선발된 HW3와 흑점찰은 향후 국내 옥수수 유전자원을 이용한 형질전환 시스템 개발연구에 이용 될 수 있을 것으로 예상된다. Immature embryos of 3 cultivars (Du Me Chal, Mi Baek Chal, Heug Jeom Chal) and 5 genotypes (HW1, KL103, HW3, HW4, KW7) were cultured on medium containing MS salts, Eriksson's vitamins, 1 mg/L 2,4-dichlorophenoxyacetic acid, 25 mM proline, 100 mg/L casamino acid, 3 mM MES, 1.7 mg/L $AgNO_3$ and 20 g/L sucrose (SIM). Frequency of somatic embryo formation on explant of immature embryos showed in HW1 (45.20%), KL103 (5.75%), HW3 (37.20%), HW4 (30.10%), KW70 (55.20%), Mi Baek Chal (18.74%), Heug Jeom Chal (22.41%), Du Me Chal (36.72%) and Hi II type (<10%), respectively. Yellowish friable embryogenic callus (YFEC) such as type II callus of Hi II genotype only produced from the HW3 and Heug Jeom Chal, whereas other cultivars and genotypes were directly formed somatic embryos with late-embryonic stages or expanded yellowish compact somatic embryo with morphological abnormality. The yellowish friable embryogenic callus (YFEC) could be proliferated on the same medium, which were maintained embryogenic capacity for 6 months over. Upon transfer to first regeneration and second regeneration medium, somatic embryos converted to plantlets at a frequency of approximately 100%. However, the expanded somatic embryos with abnormal morphology were slowly proliferated when subcultured on the same medium, and some of them were degenerated or converted to plantlets at a frequency of approximately 25%. Accordingly, The Heug Jeom Chal and HW3 genotype will be further used for development of high frequency transformation system in domestic maize germplasm.
안동규(Dong-Gyu Ahn),김진석(Jin-Suck Kim),문경제(Gyoung-Jae Moon),한길영(Gil-Young Han),문현수(Hyun-Su. Moon),윤영석(Young-Seok Yoon) 한국생산제조학회 2006 한국생산제조시스템학회 학술발표대회 논문집 Vol.2006 No.5
The objective of this research works is to develop back panel dies of PDP TV with a large size. In order to design the geometry of the dies and an initial size of blank optimally, the finite element analysis has been carried out using AUTOFORM V4.2. The conner radius of the upper trimming area and the distance from the outer line of the blank holder to the outer line of the blank have been selected as design parameters to remove the wrinkling of the trimming area. The results of the analyses have been shown that a feasible product without wrinkles and skid lines can be obtained when the conner radius ranges from 6㎜ to 8㎜ and the distance is greater than 20㎜. From the proposed design of the dies and an initial blank size, the final die set of the back panel has been successfully manufactured.
안동규(Dong-Gyu Ahn),김진석(Jin-Suck Kim),문경제(Gyoung-Jae Moon),한길영(Gil-Young Han),문현수(Hyun-Su. Moon),윤영석(Young-Seok Yoon) 한국생산제조학회 2006 한국공작기계학회 춘계학술대회논문집 Vol.2006 No.-
The objective of this research works is to develop back panel dies of PDP TV with a large size. In order to design the geometry of the dies and an initial size of blank optimally, the finite element analysis has been carried out using AUTOFORM V4.2. The conner radius of the upper trimming area and the distance from the outer line of the blank holder to the outer line of the blank have been selected as design parameters to remove the wrinkling of the trimming area. The results of the analyses have been shown that a feasible product without wrinkles and skid lines can be obtained when the conner radius ranges from 6㎜ to 8㎜ and the distance is greater than 20㎜. From the proposed design of the dies and an initial blank size, the final die set of the back panel has been successfully manufactured.
안동규(Dong-Gyu Ahn),김진석(Jin-Suck Kim),한길영(Gil-Young Han) 한국생산제조학회 2006 한국생산제조학회지 Vol.15 No.6
The objective of this research works is to develop back panel dies of PDP TV with a large size. In order to design the geometry of the dies and an initial size of blank optimally, the finite element analysis has been carried out using AUTOFORM V4.2. The conner radius of the upper trimming area and the distance from the outer line of the blank holder to the outer line of the blank have been selected as design parameters to remove the wrinkling of the trimming area. The results of the analyses have been shown that a feasible product without wrinkles and skid lines can be obtained when the conner radius ranges from 6mm to 8mm and the distance lines in the range of 40-60㎜. From the proposed design of the dies and an initial blank size, the final die set of the back panel has been successfully manufactured.
트러스형 내부구조를 가지는 샌드위치 판재의 저속 충격 특성 연구
정창균(Chang Gyun Jung),성대용(Dae-Yong Seong),양동열(Dong-Yol Yang),김진석(Jin-Suck Kim),안동규(Dong-Gyu Ahn) Korean Society for Precision Engineering 2006 한국정밀공학회지 Vol.23 No.10
Metallic sandwich plate with a truss core has metallic inner structures which have low relative density between a pair of metal skin sheets or face sheets. In this work, low impact tests have been carried out to examine the behavior of sandwich plates with a pyramidal truss core. For the low velocity impact, the impact apparatus of drop weight type has been fabricated. From the results of the experiments, maximum energy absorption is found to happen when the upper sheet fails. The sandwich plate loses its absorption ability as soon as the inner structures have been crashed completely and optimal core thickness has existed to maximize energy absorption. Comparing the metallic sandwich plate with the monocoque plate, the absorbed energy has been improved up to 160 % and the deflection decreased by up to 76%. As a result, the metallic sandwich plate with a truss core is shown to have good material for impact resistance and energy absorption.
한지숙,정상수,정혜원,정재호,허갑범,이현철,임승길,김경래,송영득,남재현,안철우,김진석,김명수,한승혁,구영석 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.1
Adrenal gland is a common site of metastatic tumors such as breast cancer, lung cancer, and colon cancer. When adrenal mass is found incidentally, adenoma is the most common among single adrenal masses. But in the case of bilateral adrenal masses, infection, bilateral metastases and hemorrhage are common. Secondary involvement of the adrenal gland is found in 25% of autopsy cases of non-Hodgkin's lymphoma. However, adrenal insufficiency is rare because it becomes apparent only when approximately 90% of adrenal cortex is destructed. We exprienced two cases of malignant lymphoma which involved the adrenal glands bilaterally. One case in which adrenal insufficiency was suspicious, was accompanied by hypovolemic shock and sepsis at the initial presentation. He died of sepsis combined with DIC even though hydrocortisone, intravenous saline infusion, and antibiotics therapy were started immediately. The other one was found incidentally, in which adrenal infiltraion was confirmed by CT scan. Hormonal level was normal and adrenal masses disappeared after chemotherapy(J Kor Soc Endocrinol 15:121-127, 2000).