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김호상(Ho Sang Kim),김창길(Chang Gil Kim),김이청(Yi Cheong Kim),김종렬(Jong Ryeol Kim) 한국마린엔지니어링학회 2010 한국마린엔지니어링학회 학술대회 논문집 Vol.2010 No.4
Artificial reefs are widely used as important means of fishing ground creation for not only commercial fishing but also recreational fishing. The evaluation of artificial reef in this study was made based upon three steps, estimation of effective bulk void volume, catch analysis per unit reef volume and biomass estimation of attachment organisms on the reef. This method can be applied to both the evaluation for post-deployment management of the exist artificial reefs and for the preservation of fisheries resource at the artificial reef fishing grounds.
Yeast $\beta$-glucan 첨가 사료가 뱀장어의 비특이적 면역 반응에 미치는 영향
김진도,우승호,김이청,이준희,조용철,최상민,박수일,Kim, Jin-Do,Woo, Sung-Ho,Kim, Yi-Cheong,Lee, Jun-Hee,Cho, Yong-Chul,Choi, Sang-Min,Park, Soo-Il 한국어병학회 2008 한국어병학회지 Vol.21 No.3
The effects of dietary yeast β-glucan administration on growth, nonspecific immune responses, serum lysozyme, skin mucous lysozyme, NBT (nitroblue tetrazolium) reduction by phagocytes, and disease resistance against Edwardsiella tarda in Japanese eel, Anguilla japonica were evaluated. Fish were fed the diets supplemented with 0%, 0.1% and 0.5% of yeast β-glucan to a commercial diet for 6 weeks. The body weight gain from the fish fed on the 0.5% supplemented diet for 6 weeks was significantly higher than the control. Both serum and skin mucous lysozyme were significantly higher in the all experimental groups except 2 weeks of 0.5% group. The bactericidal activity of serum was slightly increased at 6 weeks. Also, The intracellular superoxide anion production of kidney phagocytes was significantly higher in the all experimental groups. The diet supplemented with 0.1% were also found to raise the relative percent survival (RPS) of Japanese eel after an artificial challenge with 1×107 cells of Edwardsiella tarda per fish. The results suggested the potential of yeast β-glucan to activate some innate immune responses and to improve the growth in Japanese eel.
이배익 ( Bae Ik Lee,),김이청 ( Yi Cheong Kim ),김근업 ( Geun Up Kim ),권오남 ( O Nam Kwon ) 한국수산과학회(구 한국수산학회) 2012 한국수산과학회지 Vol.45 No.1
We compared the nutritional requirements of whole larvae of the black seabream Acanthopagrus schlegeli, sweet fish Plecoglossus altivelis, olive flounder Paralichthys olivaeus and rock fish Sebastes schlegeli. The larvae were 20, 30, 14 and 5 DAH (or spawning) of black seabream, sweet fish, olive flounder and rock fish, respectively. Specific α-amylase activity (mU/mg protein) was highest (8,324.9 mU/mg protein) in rock fish larvae (P<0.05). Specific trypsin-like protease activity was highest (11,330.1 mU/mg protein) in black seabream larvae (P<0.05), which also exhibited the highest activity, 685.5 mU/mg dry weight (P<0.05). The specific activities per mg protein and mg dry weight of black seabream were the highest (187.4 mU/mg protein and 11.3 mU/mg dry weight, respectively) (P<0.05). A/P, P/L and A/L ratios of rock fish were 1.47, 90.3 and 133.1, respectively (P<0.05). We present here basic larval digestive enzymatic nutritional requirement data.
3종의 식물 추출물에 의한 백점충의 in vitro 구제효과
김진도 ( Jin Do Kim ),김이청 ( Yi Cheong Kim ),정승희 ( Sung Hee Jung ),서정수 ( Jung Soo Seo ),박성우 ( Sung Woo Park ) 한국어병학회 2011 한국어병학회지 Vol.24 No.3
The in-vitro eliminative effects of against three types of herbal extract and formalin Ichthyophthirius multifiliis were examined. All parasites were killed within one hour after exposure to the 500 fold dilution of the complex herb extract whereas the 10 fold dilution of the fertilized solution of Salvia plebeia R. Br. killed all parasites within one hour after exposure. The 5,000 fold dilution of the extracts of Ginkgo biloba leaves killed all parasites within one hour after exposure. As a comparative agent, formalin killed all parasites within one hour at 100 ppm. As the results, the extracts of Ginkgo biloba leaves extracts have the most eliminative ability against the parasite. No differences were found among different parasite density in eliminative effects of the three types of herbal extracts and formalin. Also there were no changes in the fish gill tissues after exposure for two hours to the 5,000 fold dilution of the extract of Ginkgo biloba leaves.
김진도 ( Jin Do Kim ),양현 ( Hyun Yang ),조용철 ( Yong Chul Cho ),김이청 ( Yi Cheong Kim ),조미영 ( Mi Young Cho ) 한국환경생물학회 2010 환경생물 : 환경생물학회지 Vol.28 No.3
The pathogens and community structure of the fishes in Taehwa river were investigated from March 2007 to January 2009. During the study period, 3,504 individuals belonging to 35 species, 17 families and 9 orders were collected. The numerically dominant and subdominat species were Opsarichthys uncirostris (relative abundance 39.7%) and Hemibarbus labeo (relative abundance 30.9%). There were five Korean endemic species (20.8%) including Squalidus chankaensis tsuchigae, Zacco koreanus, Cobitis hankugensis, Coreoperca herzi, Odontobutis platycephala. The large fishes like Hemibarbus labeo or Opsarichthys uncirostris were gathered around the Samho bridge, sampling site 2 according to a season. The reaction to which two kinds of fish pathogenic virus is all negative and no fish pathogenic bacteria was isolated from 220 individuals. The fish pathogenic parasite not present variously with 7 species. Especially, Trichodina sp. was detected monthly and the infective density was high. But it is cosidered that temporary overcrowding of fish is not influenced mass mortality causing diseases in the specific site of river.
담수산 백점충(Ichthyophthirius multifiliis)에 관한 연구 II. 백점충의 인위 감염 및 어체내 충체 발달상
지보영,김기홍,박수일,김이청,Ji, Bo-Young,Kim, Ki-Hong,Park, Soo-Il,Kim, Yi-Cheong 한국어병학회 1998 한국어병학회지 Vol.11 No.1
본 연구는 내수면 양식 어류에 백점충, Ichthyophthirius multifiliis를 인위 감염시켜 감염 특성 및 어체내 충체 발달상을 밝히고자 하였다. 어종별(무지개 송어, 메기, 이스라엘 잉어), 수온별($15^{\circ}C$, $18^{\circ}C$, $21^{\circ}C$) 및 충체 발달 단계별(trophont, protomont, theront) 인위 감염 시험 결과, 어종별로는 무지개 송어 시험구에서, 수온별로는 $18^{\circ}C$ 시험구에서 그리고 충체 발달 단계별로는 theront 시험구에서 양성적인 인위 감염을 확인할 수 있었다. 그리고 theront 농도별에 있어서는 어체당 1000개체의 theront가 감염을 양성적으로 유도할 수 있는 것으로 조사되었으며 어체당 1500개체 이상의 theront는 무지개 송어치어에 100%의 감염을 유도하였다. 수온 $18^{\circ}C$의 인위 감염된 무지개 송어에서 7일 동안 어체내 충체의 발달 과정을 조사한 결과, 충체는 시간이 경과함에 따라 연속적인 발달을 행하여 크기가 증대되었으며 감염 3일째부터는 성숙되는 것으로 확인되었다. 그리고 총체의 형태학적 변화는 감염 2일째에는 구부장치가 기능적으로 발달하기 시작한 것을 볼 수 있었고, 감염 4일째부터는 수축포가 현저히 발달되기 시작하였으며 감염 6일째는 이들의 수가 현저히 증가한 것을 확인할 수 있었다. 그리고 감염 기간이 경과함에 따라 충체는 숙주의 아가미 일차 새변의 입새 동맥쪽으로 이동하는 경향을 나타내었다. Concerned to the lyfe cycle of Ichthyophthirius multifiliis, the experimental infection and development of the parasites were studied in the several freshwater cultured fishes. Opitimum conditions for the propagation of the parasite by serial passage with the rainbow trout fry was observed. Visiable white spots were examined in the body surface, fins and gills of the healthy fries, and a stable infection has been maintained for 2 months in the experimental system (Temperature: $18{\pm}1^{\circ}C$ DO: 7-7.5 ppm; pH: $7{\pm}0.2$). Induction conditions for artificial infection of the parasite by interms of the host fishes, stages of the parasites, and rearing temperature regimes was investigated. Rainbow trout fries showed a positive infection which was resulted from exposure of theront at $18^{\circ}C$. The rainbow trout fries induced white spots on the body surface at 3-7 days exposure to the theronts at $18^{\circ}C$. It was found that exposure of the rainbow trout fries exposed to 1,000 theronts per fish (10 theront/ml) for 45-60 minutes at $18^{\circ}C$ would consistently produce infection. Perfect infection (100%) was induced when the fries were exposed to 1,500 theront per fish (15 theront/ml) under laboratory condition. Development of I. multifiliis in the rainbow trout was observed for 7 days postexposure (PE). The parasite increased in average diameter from $54{\mu}m$ on the 1st day PE to $426{\mu}m$ on the 7th day PE. In the initial infestation period, the parasites were found on the gill epithelium, and on the 3rd day PE they invaded into the basal part of the gill filament adjacent to the major blood vessels, particularly the afferent vessels. Morphological change of buccal apparatus were observed on the 2nd day PE. Contractile vacuoles were more prominent on the 4th day PE, and they had notable changes on the 7th day PE.