난소암 환자 혈장에서 미세위성 표지자를 이용한 이형접합소실 분석

김용민 ( Yong Min Kim ),김영태 ( Young Tae Kim ),이규완 ( Kyu Wan Lee ) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.9

Objective : Recent studies demonstrated that soluble tumor DNA is found in the plasma of cancer patients, and some microsatellite alteration have been identified in ovarian carcinoma. The aim of study was to detect microsatellite abnormalities in the plasma of patients with ovarian carcinoma and to evaluate their efficacy as molecular screening or diagnostic tool for ovarian cancer. Methods : In fifteen ovarian carcinoma patients, DNA was extracted from the plasma samples and microsatellite analysis was done with 11 microsatellite markers. Results : All fifteen cases showed at least one tumor specific alteration in microsatellite analysis. The frequency of genetic alteration varies from 14.2% to 85.7%. Highly frequent tumor specfic alteration markers are D18S69 (85.7%), D10S215 (69.2%), D16S504 (66.7%), D8SNEFL (62.5%) and D11S1340 (60.0%). Conclusion : These results suggest that the mutation of tumor DNA can be detected in plasma of patients with ovarian carcinoam. LOH is more frequent event and the frequency of genetic alteration is relatively higher than that of previous reports.

호흡기 ; 중증패혈증 및 패혈증쇼크 환자에게서 파종성혈관내응고의 빈도 및 예후

김용민 ( Yong Min Kim ),김동규 ( Dong Gyu Kim ),김성열 ( Seong Yeol Kim ),이석원 ( Seok Won Lee ),황용일 ( Yong Il Hwang ),권영석 ( Young Seok Kwon ),안영환 ( Young Hwan An ),정기석 ( Ki Suck Jung ),장승훈 ( Seung Hun Jang ),장길 대한내과학회 2010 대한내과학회지 Vol.79 No.5

Background/Aims: We investigated the incidence and prognosis of disseminated intravascular coagulation (DIC) using DIC scoring system in patients with severe sepsis or septic shock. Methods: Patients admitted to the intensive care unit (ICU) in a tertiary hospital for severe sepsis or septic shock were enrolled from Mar. 2008 to Feb. 2009. Using the International Society on Thrombosis and Haemostasis (ISTH) criteria, we calculated DIC score at three time points (Day 0, Day 1, and Day 2). Results: Among 111 patients with severe sepsis or septic shock, 89 (severe sepsis, 8; septic shock, 81) were enrolled. Mean DIC score at ICU admission was 3.3±1.3 and the incidence of overt DIC (DIC score ≥ 5) during the first 48 hours was 33.8% (27/89). Sequential Organ Failure Assessment (SOFA) score was well correlated with DIC score and was higher in patients with overt DIC than in those without. The ICU, hospital and 28-day death rates in patients with overt DIC were 63.0%, 66.7%, and 63.0%, respectively, which were significantly higher than in those without overt DIC. In multivariate analysis, Simplified Acute Physiology Score (SAPS) II was significantly associated with hospital death (p=0.002), and the occurrence of overt DIC showed a borderline significance (p=0.053). Conclusions: Using the ISTH criteria for DIC, the incidence of overt DIC was 33.8% in patients with severe sepsis or septic shock. The occurrence of overt DIC was associated with a higher organ failure score and hospital outcomes. (Korean J Med 79:526-535, 2010)

지방합성 인자 조절 및 식이 섭취 감소를 통한 곽향의 항비만 효능

김용민 ( Young Min Kim ),김미혜 ( Mi Hye Kim ),양웅모 ( Woong Mo Yang ) 한방비만학회 2015 한방비만학회지 Vol.15 No.2

Objectives: The herb of Agastache rugosa (AR) is a traditional herbal medicine used for colds, vomiting and furuncles. However, there are few reports to investigate the inhibitory effects of AR on obesity. In this study, the effects of AR on high fat diet (HFD)-induced obesity and its mechanism of actions were investigated in experimental animals. Methods: The mice were fed HFD for 4 weeks to induce obesity. After randomly divided into normal fat diet, HFD and AR groups, 200 mg/kg of AR was administrated for 4 weeks with continuous HFD feeding while vehicle was orally treated to HFD group. Food intake and body weight were recorded weekly. Results: Increased body weight by HFD was improved by AR treatment. AR administration inhibited an increase of visceral fat weight as well as adipocyte hypertrophy. Hepatic steatosis was ameliorated in AR-treated mice. In addition, treatment of AR attenuated the expression of adipogenic transcription factor, peroxisome proliferator-activated receptor (PPAR)-gamma in the epididymal adipose tissue. Also the increased serum leptin level by HFD was maintained in AR group, leading to inhibition of food intake. Conclusions: AR treatment showed inhibitory effects on HFD-induced obesity by inhibition of PPAR-gamma and reduction of food intake. AR could be an alternative treatment for obesity.

근사화된 기울기 히스토그램 서술자를 이용한 보행자 검출

김용민(Yong Min Kim),김봉모(Bong Mo Kim),박기태(Ki Tae Park),문영식(Young Shik Moon) 한국정보과학회 2011 정보과학회논문지 : 소프트웨어 및 응용 Vol.38 No.7

본 논문에서는 보행자 탐지를 위해 근사화된 HOG(Histograms of Oriented Gradients) 서술자를 이용하는 방법을 제안한다. HOG 서술자는 보행자를 검출하는데 사용된다. 그러나 HOG 서술자를 계산하는 것은 시간 복잡도가 높기 때문에 실시간으로 보행자를 검출하기 어렵다. 따라서, 본 논문에서는 검출 정확도에 대한 저하없이 실시간 처리를 위해 근사화된 HOG 기술자를 제안한다. 이를 위해 기존의 방법의 분석을 통해 계산 부분 중 기울기와 방향성을 계산하는 부분을 근사화한다. 실험 결과는 기존의 방법과 비교하여 29%의 계산 시간이 개선되었음을 보였다. In this paper, we propose a method of pedestrian detection based on approximated histograms of oriented gradients(HOG). A conventional HOG descriptor is widely used for detecting pedestrians in video sequences. However, since its time complexity is very high, it is not possible to detect pedestrians in real time. Therefore, we propose an approximated HOG descriptor for reducing execution time of detecting pedestrians without degrading detection accuracy. To this end, approximation of gradients and orientations is performed by analyzing a conventional HOG method. Experimental results have shown that our method reduce 29% of execution time, compared with conventional method.

지하철 2호선 역세권 별 유출입특성과 역세권 토지이용에 관한 연구

김용민(Yong-min Kim),김환배(Hwanbae Kim),정혜영(Hye-Young Cheong),이명훈(Myeong-Hun Lee) 한국지역개발학회 2017 韓國地域開發學會誌 Vol.29 No.3

This study proposes rational development and management of station area in terms of users, analyzing the flow rate of passengers and land use. and we compared 2030 Seoul City Plan to suggest the management plan considering characteristics of users of stations. First, it was classified into 4 types according to the characteristics of the time-use flow. Type A: influxes in the morning, and outflows in the afternoon and night, Type B: outflows in morning and influx at afternoon and night, Type C: outflows in the morning and night influx in the afternoon, Type D: a case where the flow is similar in both morning, afternoon, and night. Land use characteristics according to building use were analyzed. Type A is classified as business and commerce type, type B as residential type, type C as residential commercial type, and type D as commercial type. Third, the development density realized in four types of usage areas. Type A (89.1%) and Type B (86.3%) were found to have lower capacity of density than Type C (77.2%) and Type D (75.4%).

다단계 임계치를 이용한 마크형 디지털 워터마킹

김용민(Yong-Min Kim),김현환(Hyun-Hwan Kim),김두영(Doo-Young Kim) 한국통신학회 1999 한국통신학회 학술대회논문집 Vol.1999 No.7

상기저형 인두피판을 이용한 범인두부전 환자의 치료

김용민 ( Young Min Kim ) , 박광범 ( Gwang Bum Park ) , 김학균 ( Hak Kyun Kim ) 조선대학교 구강생물학연구소 2008 Oral Biology Research (Oral Biol Res) Vol.32 No.1

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어성초(Houttuynia cordata Thunb) 유산균 발효물의 항산화 활성

김용민(Yong-Min Kim),정해진(Hae-Jin Jeong),정헌식(Hun-Sik Chung),성종환(Jong-Hwan Seong),김동섭(Han-Soo Kim),김한수(Dong-Seob Kim),이영근(Young-Guen Lee) 한국생명과학회 2016 생명과학회지 Vol.26 No.4

본 연구에서는 항산화 활성을 가지고 있는 것으로 이미 알려진 어성초의 효능을 더욱 증가시켜 고부가가치를 지닌 기능성 원료로써 활용하고자, Leuconostoc mesenteroides 4395와 Lactobacillus sakei 383 두 유산균을 이용하여 발효시킨 후 그 추출물의 항산화 활성을 측정하였다. 항산화 활성 척도로써 DPPH 라디칼 소거능을 측정하였는데 10일간의 발효에서 두 균주 모두 발효 5일차에, 발효온도는 Leuconostoc mesenteroides 4395가 30℃에서 71.67±0.52%, Lactobacillus sakei 383는 35℃에서 70.11±0.67%로 각각 최대의 활성이 나타났으며, 두 가지 균 모두 대조군인 미발효물에 비해 DPPH 라디칼 소거능이 약 20% 상승하였다. 총 페놀함량에서는 대조군이 35.90±0.61 mg/g 함유한 반면에 발효한 후 두 균주 모두 약 10 mg/g씩 증가하였고, 총 플라보노이드 함량에서도 대조군의 21.69±1.52 mg/g에 비해 약 6 mg/g 이상 증가함을 나타내었다. SOD 유사활성에서는 발효의 효과가 크게 나타나지 않았으나, 대조군에 비해서는 미약하게 증가한 활성을 나타내었다. 이러한 결과들은 어성초의 유산균 발효물이 발효하지 않은 어성초에 비해 항산화 활성이 더욱 높아, 고부가가치를 지닌 기능성식품의 원료로써 활용할 가능 성이 있는 것으로 판단되었다. This study was performed to evaluate the possibility of application of lactic acid bacteria fermentation to increase the anti-oxidative activity of extracts from Houttuynia cordata Thunb. Houttuynia cordata Thunb. was fermented by two species of lactic acid bacteria, Leuconostoc mesenteroides 4395 and Lactobacillus sakei 383. The anti-oxidative activities of Houttuynia cordata Thunb. extracts were analyzed both before and after fermentation. Anti-oxidative activity was determined by in vitro assays to measure 1,1-diphenyl- 2-picrylhydrazyl (DPPH) scavenging and super oxide dismutase (SOD)-like activities, and by determining total flavonoid and total phenolic compound contents. The extracts of fermented Houttuynia cordata Thunb. had higher anti-oxidative activity than the unfermented control. The DPPH scavenging activity of the extracts after fermentation by Leuconostoc mesenteroides 4395 at 30℃ for 5 days was 71.67±0.52%, and after Lactobacillus sakei 383 fermentation at 35℃ for 5 days was 70.11±0.67%; these activities were both about 20% higher than the control. Increases of about 10 mg GAE/g of total phenolic compounds were found in both fermented extracts and both contained about 6 mg quercetin equivalents/g of total flavonoids, compared with 35.90±0.61 mg/g and 21.69±1.52 mg/g in the control, respectively. These results also suggested that fermentation time and temperature were important factors in determining the anti-oxidative effect of extracts from fermented Houttuynia cordata Thunb. These findings should be valuable for the development of medicines or functional foods with antioxidative activity.

견관절경 수술 후 초기 통증에 대한 사각근간 차단 마취술의 유용성

김용민(Yong-Min Kim),박경진(Kyoung-Jin Park),김동수(Dong-Soo Kim),최의성(Eui-Sung Choi),손현철(Hyun-Chul Shon),조병기(Byung-Ki Cho),신영덕(Young-Duck Shin),배승환(Seung-Hwan Bae) 대한정형외과학회 2011 대한정형외과학회지 Vol.46 No.4

목적: 견관절의 관절경 수술은 그동안 전신 마취하에 주로 실시되었는데, 수술 후 초기 통증이 매우 심할 뿐 아니라 잘 조절되지 않았다. 이에 본 연구에서는 견관절경 수술에 있어 상완신경총 사각근간 차단술 마취를 전신 마취와 비교하여 상완신경총 사각근간 차단술의 술 후 초기 통증 조절의 유용성에 대해 알아보고자 하였다. 대상 및 방법: 2008년 9월부터 2009년 3월까지 회전근개 파열로 견관절경 수술을 시행받은 환자 가운데 40예를 대상으로 전향적, 무작위 검사를 실시하였다. 상완신경총 사각근간 차단 마취하에 수술한 20예, 전신 마취하에 수술한 20예로 나누었으며 두 군의 수술 전과 수술 후 3일간의 통증 정도를 visual analogue scale (VAS)을 이용하여 측정하였다. 그리고 입원 중 자가진통조절기(patient controlled analgesia, PCA)의 사용 시간과 추가적인 진통제 사용량을 조사하였다. 그밖에 두 군 간의 자세적인 차이와 자세에 따른 합병증을 조사하였다. 그리고 유의성을 알기 위해 이원 반복 측정 분산 분석으로 통계학적 분석을 하였다. 결과: 두 군간의 수술 전 VAS score는 유의한 차이를 보이지 않았으며 상완 신경총 사각근간 차단 마취군의 경우 수술 직후부터 수술 후 48시간까지 VAS score가 전신 마취군과 비교하여 유의하게 적었다. 입원 중 PCA의 사용기간은 사각근간 차단 마취군에서 유의하게 길었음을 확인할 수 있었고 추가적인 진통제 사용은 사각근간 차단 마취군에서 유의하게 적었다. 결론: 상완 신경총 사각근간 차단 마취후 시행한 견관절경 수술은 수술 후 48시긴 간의 통증이 유의하게 적어 환자의 초기 재활치료를 수월하게 시행할 수 있었고 추가적인 진통제 사용 또한 적어 진통제로 인한 부작용을 최소화 할 수 있었다. 따라서 상완 신경총 사각근간 차단마취는 견관절경 수술에 있어 수술 초기 통증을 효과적으로 감소시킬 수 있는 좋은 마취법이라 생각된다. Purpose: After shoulder arthroscopy via general anesthesia, most patients complain of severe pain during the early post operative period. In this study, the efficacy of pain control during the early post operative period with interscalene block anesthesia for shoulder arthroscopy was investigated and compared with general anesthesia. Materials and Methods: A prospective randomized controlled study was conducted on 40 patients who underwent shoulder arthroscopy between September 2008 and March 2009. The patients were grouped according to the method of anesthesia. The visual analogue scale (VAS) at the preoperative and early postoperative periods was checked and compared. In addition, the duration of patient controlled analgesia (PCA) usage and additional pain killer injections were examined. Results: There was no significant difference between the two groups for the VAS score before surgery, but the interscalene block group showed a significantly lower VAS score. The duration of PCA usage was also significantly longer for the interscalene block group, which implies less pain The number of additional pain killer injections was significantly less in the interscalene block group. Conclusion: The patients who underwent shoulder arthroscopy with interscalene block had significantly less pain during the immediately post operative period for up to 48 hours. So patients could conduct initial rehabilitation and experience minimized side effects caused by analgesics due to the decreased use of pain killer. Therefore, interscalene block is considered a good method of anesthesia for the immediate postoperative pain control after shoulder arthroscopy.

드라이버 척도 개발 및 타당화

김용민(Yong-Min KIM),김미나(Mi-Na KIM),장은호(Eun-Ho JANG),손영식(Young-Sik SON) 한국상담심리교육복지학회 2020 상담심리교육복지 Vol.7 No.4

본 연구는 기존의 드라이버 척도의 보완점을 개선하여 보다 타당화된 드라이버 척도를 개발하는 데 목적이 있다. 본 연구는 사전연구를 실시하여 예비문항을 확정했으며, 552명의 표본으로 탐색적 요인분석을 실시했다. 그 결과, 요인적재량, 교차적재량, 공통성, 신뢰도를 충족하는 5요인 25문항이 도출되었다. 이후 탐색적 요인분석에서 확인한 5요인 구조의 안정성을 확보하고, 이 구조가 다른 집단에서도 확인되는지 알아보기 위해 340명을 대상으로 확인적 요인분석을 실시했다. 모형적합도를 확인한 결과, 주요지표인 CMIN/DF와 RMSEA, 보조지표인 TLI와 CLI가 기준을 충족하여 요인 구조가 확인되었다. 또한 준거 관련 타당도를 검정하기위해 임상적 회복탄력성 척도와 상관을 확인한 결과 유의미한 상관을 보였다. 마지막으로 본 연구의 의의와 후속 연구에 대해 제언했다. The purpose of this study is to develop a more valid driver inventory by improving the complementary points of the existing driver inventory. In this study, preliminary research was conducted to determine preliminary questions, and exploratory factor analysis was performed with a sample of 552 people. As a result, 25 questions with 5 factors satisfying factor loading, cross loading, commonality, and reliability were derived. Afterwards, a confirmatory factor analysis was conducted on 340 people to ensure the stability of the five-factor structure identified in the exploratory factor analysis and to see if this structure was also confirmed in other groups. As a result of checking the model suitability, the factor structure was confirmed as the main indicators CMIN/DF and RMSEA, and the auxiliary indicators TLI and CLI met the criteria. In addition, in order to test the validity of the criterion, the correlation was confirmed with the clinical resilience inventory and showed a significant correlation. Finally, the significance of this study and subsequent studies were suggested.