저장유통 조건에 따른 오징어 품질예측 모델링

김소이,신지영,김현석,양지영,Kim, So-i,Shin, Jiyoung,Kim, Hyunsuk,Yang, Ji-young 한국식품위생안전성학회 2022 한국식품위생안전성학회지 Vol.37 No.3

There are a number of methods to evaluate the quality of squid. However, when purchasing the fish, consumers and retails rely only on the sensory test and flavor in the field. Therefore, this study was aimed to prove relationship between scientific indicator and sensory test. Total viable cell count (TVC), viable cell count of Pseudomonas spp., pH and volatile basic nitrogen (VBN) were selected as scientific indicators and mesured during the storage of squid at different temperature. The squid was storaged at 3 different temperature (5℃, 15℃, 20℃). Off flavor determination time was measured by R-index, and kinetic modeling was conducted. Activation energies of off-flavor determination time, TVC, Pseudomonas spp, VBN, and pH were 51.210 kJ/mol, 42.88 kJ/mol, 50.283 kJ/mol, 72.594 kJ/mol and 41.99 kJ/mol respectively. Activation energy of off-flavor determination time was approximated to viable cell count of Pseudomonas spp., TVC, pH and VBN as an order. Especially, viable cell count of Pseudomonas spp. had best match of the activation energy. Therefore, it was judged that indicator of off-flavor determine time was viable cell count of Pseudomonas spp..

상수리나무 잎 추출물의 항균 및 항산화 활성과 활성 물질 분리

김소이 ( So I Kim ),안유진 ( You Jin Ahn ),김은희 ( Eun Hee Kim ),박수남 ( Soo Nam Park ) 대한화장품학회 2009 대한화장품학회지 Vol.35 No.2

본 연구에서는 상수리나무 잎 추출물의 피부 상재균에 대한 항균작용과 항산화, 성분 분석 및 tyrosinase, elastase 저해 효과에 관한 조사를 수행하였다. 피부 상재균에 대한 항균활성 측정결과, S. aureus, P. acnes, P. ovale, E. coli에 대한 ethyl acetate 분획의 MIC는 각각 0.13 %, 0.25 %, 0.13 %, 0.25 %로 나타났으며, S. aureus, P. acnes, P. ovale에서 큰 항균활성을 나타내었다. 추출물의 free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성(FSC₅₀)은 50 % ethanol 추출물(12.13 μg/mL) < ethyl acetate 분획(7.07) < 당을 제거시킨 플라보노이드 aglycone 분획(6.20) 순으로 증가하였다. Luminol-의존성 화학발광법을 이용한 Fe³⁺-EDTA/H₂O₂ 계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 상수리나무 잎 추출물의 총항산화능은 50 % ethanol 추출물(OSC₅₀, 1.81 μg/mL) < ethyl acetate 분획(1.70) < aglycone 분획(0.70)순으로, aglycone 분획에서 가장 큰 활성을 나타내었다. 상수리나무 잎 추출물에 대하여 rose-bengal로 증감된 사람 적혈구의 광용혈에 대한 억제 효과를 측정하였다. 상수리나무 잎 추출물의경우 농도 의존적(1 ~ 25 μg/mL)으로 광용혈을 억제하였다. 특히 당을 제거시킨 aglycone 분획은 25 μg/mL 농도에서 τ50이 220.00 min으로 매우 큰 세포보호 효과를 나타내었다. 상수리나무 잎 추출물 중 ethyl acetate 분획의 당 제거 반응후 얻어진 aglycone 분획은 TLC에서 3개의 띠로 분리되었고 상수리나무 잎 추출물의 ethyl acetate 분획의 TLC 크로마토그램은 4개의 띠(QA 1 ~ QA 4)로 분리되었으며, 그 중 QA 1은 kaempferol, QA 2는 quercetin, 그리고 QA 3는 gallic acid로 확인되었다. Aglycone 분획의 tyrosinase 저해활성(IC50)은 65.67 μg/mL이었고, elastase 저해활성(IC₅₀)은 24.50 μg/mL이었다. 이상의 결과들은 상수리나무 잎 추출물이 ¹O₂ 혹은 다른 ROS를 소광시키거나 소거함으로써 그리고 ROS에 대항하여 세포막을 보호함으로써 생체계, 특히 태양 자외선에 노출된 피부에서 항산화제로서 작용할 수 있음을 가리키며, 상수리나무 잎 성분 분석, aglycone 분획의 tyrosinase, elastase 저해활성 그리고 피부 상재균에 대한 항균작용으로부터 항산화, 항노화 및 항균성 화장품 소재로서의 응용 가능성을 확인하였다. In this study, the antibacterial activity, antioxidative effects, inhibitory effects on tyrosinase, inhibitory effects on elastase, and components of Quercus acutissima Carruth leaf extracts were investigated. MIC values of ethyl acetate fraction from Q. acutissima Carruth leaf on P. acnes, S. aureus, P. ovale, and E. coli were 0.13 %, 0.25 %, 0.13 % and 0.25 %, respectively. The results showed that the antibacterial activity of the ethyl acetate fraction was the highest in the S. aureus, P. acnes, and P. ovale. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC₅₀) of extract/fractions of Q. acutissima Carruth. leaf was in the order: 50 % ethanol extract (12.13 μg/mL) < ethyl acetate fraction (7.07 μg/mL) < deglycosylated flavonoid aglycone fraction (6.20 μg/mL). Reactive oxygen species (ROS) scavenging activities (OSC₅₀) of some Q. acutissima Carruth leaf extracts on ROS generated in Fe³⁺-EDTA/H₂O₂ system were investigated using the luminol-dependent chemiluminescence assay. The order of ROS scavenging activity was 50 % ethanol extract (OSC50, 1.81 μg/mL) < ethyl acetate fraction (1.70 μg/mL) < deglycosylated flavonoid aglycone fraction (0.70 μg/mL). Deglycosylated flavonoid aglycone fraction showed the most prominent scavenging activity. The protective effects of extract/fractions of Q. acutissima Carruth leaf on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The Q. acutissima Carruth leaf extracts suppressed photohemolysis in a concentration dependent manner, particularly deglycosylated flavonoid aglycone fraction exhibited the most prominent celluar protective effect (τ50, 220.00 min at 25 μg/mL). Aglycone fractions obtained from the deglycosylation reaction of ethyl acetate fraction among the Q. acutissima Carruth leaf extracts, showed 3 bands (QA 1, QA2 and QA3) on TLC. TLC chromatogram of ethyl acetate fraction of Q. Carruth. leaf extract revealed 4 bands (QA 1 ~ QA 4), Among them, kaempferol (QA 1), quercetin (QA 2), and gallic acid (QA 3) were identified. The inhibitory effect (IC50) of aglycone fraction on tyrosinase was 65.7 μg/mL. The inhibitory effect (IC50) of aglycone fraction on elastase was 24.50 μg/mL. These results indicate that extract/fractions of Q. acutissima Carruth. can functionized as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging 1O2 and other ROS, and protect cellular membranes against ROS. Extract/fractions of Q. acutissima Corruth can be applicable to new functional cosmetics for antioxidant, antiaging, antibacterial activity.

Evaluation of Flat fish (Glyptocephalus stelleri) Freshness Using Comparison Sensory test and Chemical Analysis

So-I Kim(김소이),Ji-young Shin,Yun-hyeong Lee,Jun-ho Yang,I-seul Choi,Eun-ji Cha,Hyun-Suk Kim,So-Ra Lee,Ji-young Yang 한국수산과학회 양식분과 2020 한국수산과학회 양식분과 학술대회 Vol.2020 No.7

만성신부전 : 만성콩팥병 환자에서 AST-120이 혈관내피세포 기능부전에 미치는 영향 및 기전

김소이 ( So I Kim ),송경희 ( Kyung Hui Song ),유민아 ( Min A Yu ),류동열 ( Dong Ryeol Ryu ),김승정 ( Seung Jung Kim ),최규복 ( Kyu Bok Choi ),신길자 ( Gil Ja Shin ),윤견일 ( Kyeon Il Yoon ),강덕희 ( Duk Hee Kang ) 대한신장학회 2008 춘계학술대회 초록집 Vol.28 No.1

함초(나문재 및 퉁퉁마디) 추출물의 항산화 작용에 관한 연구(I)

전소미 ( So Mi Jeon ),김소이 ( So I Kim ),안정엽 ( Jeung Youb Ahn ),박수남 ( Soo Nam Park ) 대한화장품학회 2007 대한화장품학회지 Vol.33 No.3

본 연구에서는 함초 추출물(나문재 및 퉁퉁마디)의 항산화능을 조사하였다. 나문재 추출물의 free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성(FSC₅₀)은 100 % ethanol 추출물(329.33 g/mL) < 50 % ethanol 추출물(40.73) < ethylacetate 분획(13.87) < ethylacetate 분획에서 당을 제거시킨 aglycone 분획(7.80) 순으로 증가하였다. 퉁퉁마디의 경우, free radical 소거활성은 ethylacetate 분획 및 aglycone 분획이 각각 23.21 및 28.50 g/mL이었다. Luminol-의존성 화학발광법을 이용한 Fe³⁺- EDTA/H₂O₂ 계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 나문재 추출물의 총항산화능은 50 % ethanol 추출물(OSC₅₀, 0.99 g/mL) < ethylacetate 분획(0.05) < aglycone 분획(0.03)순으로, aglycone 분획에서 가장 큰 활성을 나타내었다. 퉁퉁마디의 경우, ethylacetate 분획 및 당을 제거시킨 aglycone 분획의 ROS 소거활성은 각각 0.10 및 0.20 g/mL이었다. 나문재 및 퉁퉁마디 추출물에 대하여 rose-bengal로 증감된 사람 적혈구의 광용혈에 대한 억제 효과를 측정하였다. 100 % ethanol 나문재 추출물의 경우 농도 의존적(1 ∼ 100 g/mL)으로 광용혈을 억제하였다. 특히 ethylacetate 분획에서 당을 제거시킨 aglycone 분획은 50 g/mL 농도에서 τ₅₀이 310 min으로 매우 큰 세포보호 효과를 나타내었다. 퉁퉁마디의 경우는 ethylacetate 분획에서 비교적 큰 세포보호 활성을 나타내었다. 이상의 결과들은 나문재 추출물이 ¹O₂ 혹은 다른 ROS를 소광시키거나 소거함으로써 그리고 ROS에 대항하여 세포막을 보호함으로써 생체계, 특히 태양 자외선에 노출된 피부에서 항산화제로서 작용할 수 있음을 가리킨다. In this study, the antioxidative effects of Sueada asparagoides and Salicornia herbacea extracts were investigated. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC₅₀) of extract/fractions of Sueada asparagoides was in the order: 100 % ethanol extract (329.33 g/mL) < 50 % ethanol extract (40.73) < ethylacetate fraction (13.87) < deglycosylated aglycone fraction (7.80). In case of Salicornia herbacea, the free radical scavenging activities of ethylacetate fraction and aglycone fraction were 13.87 and 7.80 g/mL, respectively. Reactive oxygen species (ROS) scavenging activities (OSC₅₀) of Sueada asparagoides and Salicornia herbacea extracts on ROS generated in Fe³⁺-EDTA/H₂O₂ system were investigated using the luminol-dependent chemiluminescence assay. The order of ROS scavenging activity of Sueada asparagoides extracts was 50 % ethanol extract (OSC₅₀, 0.99 g/mL) < ethylacetate fraction (0.05) < aglycone fraction (0.03). Aglycone fraction showed the most prominent scavenging activity. In case of Salicornia herbacea, the ROS scavenging activities of ethylacetate fraction and aglycone fraction were 0.10 and 0.20 g/mL, respectively. The protective effects of extract/fractions of Sueada asparagoides and Salicornia herbacea on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethanol extract (100 %) of Sueada asparagoides diminished photohemolysis in a concentration dependent manner (1 ∼ 100 g/mL). Particularly deglycosylated aglycone fraction exhibited the most prominent celluar protective effect (τ₅₀, 310 min at 50 g/mL). In case of Salicornia herbacea, ethylacetate fraction exhibited more potent protective effect. These results indicate that extract/fractions of Sueada asparagoides can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging ¹O₂ and other ROS, and protect cellular membranes against ROS.

제주 자생 식물 추출물의 항산화 및 세포 보호작용에 관한 연구(I)

박수남 ( Jung Won Choi ),최정원 ( So I Kim ),김소이 ( So Mi Jeon ),전소미 ( Jill Young Kim ),김진영 ( Hee Jung Yang ),양희정 ( Keun Ha Lee ),이근하 ( Soo Nam Park ) 대한화장품학회 2006 대한화장품학회지 Vol.32 No.3

본 연구에서는 제주도에서 자생하는 36종의 식물 추출물에 대한 항산화, 미백 그리고 주름억제 효능을 조사하였다. 항산화 효능은 DPPH (1,1-diphenyl-2-picrylhydrazyl radical)을 이용한 free radical 소거 활성 측정, luminol-의존성 화학발광법을 이용한 Fe³⁺-EDTA/H₂O₂ 계에서 생성된 활성산소종(ROS)의 소거활성 측정, rose-bengal로 증감된 사람 적혈구의 광용혈법을 이용한 세포보호 효과를 측정하였다. 미백 효능 검색으로 tyrosinase 저해활성 측정과 주름개선 효능검색으로 elastase의 저해 활성을 측정하였다. 그 결과 DPPH의 free radical 소거활성은 소리쟁이 전초(Rumex crispus FSC₅₀; 10μg/mL)에서 높게 나타났고, 활성산소 소거활성은 질경이 전초(Plantago asiatica, OSC₅₀; 0.006μg/mL), 소리쟁이 전초(0.04 μg/mL), 세포보호활성은 50 μg/mL 농도에서 소리쟁이 전초(τ₅₀; 1,140 min, 후박나무 잎(Machilus thunbergii, 216 min) 및 노박덩굴 잎/줄기/꽃(Celastrus orbiculatus, 200 min)에서 크게 나타났다. 200 μg/mL 농도에서 tyrosinase 저해활성은 뽕나무 줄기(Morus alba, 94.8%), elastase의 저해 작용은 소리쟁이 전초(81.8%), 뽕나무 줄기(74.6%), 노박덩굴 잎/줄기/꽃(63.1%)에서 큰 활성이 나타났다. 결론적으로 소리쟁이, 질경이, 후박나무 잎, 뽕나무 줄기 및 노박덩굴 잎/줄기/꽃 추출물은 기능성 화장품 제조를 위한 원료로 사용하기에 충분한 가능성이 있음을 확인할 수 있었다. 향후 제품화를 위하여 좀더 다양한 연구들이 필요하다고 사료된다. In this study, we investigated the anti-oxidative, anti-wrinkle and whitening effects of 36 plant extracts collected from self-growing plants in Jeju island. Their anti-oxidant activities were measured by free radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl radical), reactive oxygen species (ROS) scavenging activities on ROS generated in Fe³⁺-EDTA/H₂O₂ system using the luminol-dependent chemiluminescence assay, and cell protecting activities using the rose-bengal sensitized photohemolysis of human erythrocytes. In addition, the inhibitory activities of tyrosinase for whitening effect and elastase for anti-wrinkle were investigated. The results showed that the Rumex crispus (all grass) extract has the most significant free radical scavenging activity (FSC₅₀; 10 μg/mL), Plantago asiatica and Rumex crispus extracts for the prominent ROS scavenging activity (OSC₅₀; 0.006 μg/mL, 0.04 μg/mL respectively), Rumex crispus (τ₅₀; 1,140 min at 50 μg/mL), Machilus thunbergii leaf (216 min), and Celastrus orbiculatus (200 min) for cell protecting effects, Morus alba stem for the inhibitory activity on tyrosinse (94.8% at 200 μg/mL), Rumex crispus (81.8% at 200 μg/mL), Morus alba (74.6%), and Celastrus orbiculatus leaf/stem/flower (63.1%) for the activity on elastase. These results indicated that the extracts of Rumex crispus, Plantago asiatica, Machilus thunbergii leaf, Morus alba stem, Celastrus orbiculatus leaf/stem/flower could have the functional effects when they are added as ingredients in cosmetics. Thus, it is concluded that further experiments are needed to apply for cosmetic products.

식도부식제 손상으로 결장식도 성형술 후 발생한 경부 가성게실과 과잉결장

최윤정 ( Yun Jung Choi ),김소이 ( So I Kim ),정혜경 ( Hye Kyung Jung ),이주호 ( Ju Ho Lee ),김성은 ( Seong Eun Kim ),정성애 ( Sung Ae Jung ),유권 ( Kwon Yoo ),문일환 ( Il Hwan Moon ) 대한소화기기능성질환·운동학회 2009 Journal of Neurogastroenterology and Motility (JNM Vol.15 No.1

Corrosive injury of the esophagus tends to occur more commonly due to intentional ingestion of corrosive substances, rather than due to accidents or as a complication of esophageal diseases. Long-term complications of esophageal corrosive injury include esophageal stricture, antral stenosis and rarely, the development of esophageal carcinoma. When esophageal stricture occurs, initial treatment is performed by repetitive bougie dilatation and balloon catheter dilation. However, in cases with severe esophageal stricture, esophageal dilatation is ineffective and esophageal reconstructive surgery is necessary. The colon is the most common organ used to substitute for the esophagus. An appropriate blood supply is crucial to maintain its function. The propulsive contraction, of the distal colon as a substitute, is an especially important force needed to propel a bolus into the stomach. However, a motility disturbance in this section may produce redundancy of the graft or a pseudodiverticulum at the proximal anastomosis site of the esophagoplasty, resulting in complications such as food retention. We treated a patient who presented with a cervical pseudodiverticulum and redundancy of the intra-thoracic colon graft three years after coloesophagoplasty. Revision surgery was performed to relieve the debilitating symptoms including dysphagia, nausea, vomiting, and severe weight loss. The surgery improved the patient`s quality of life. (Kor J Neurogastroenterol Motil 2009;15:70-75)

분말형 견운모를 혼입한 모르타르의 내화성능 연구

박지연(Ji-Yeon Park),김소이(So-I Kim),김성하(Seong-Ha Kim),이재용(Jae-Yong Lee),정철우(Chul-Woo Chung) 한국건설순환자원학회 2021 한국건설순환자원학회 논문집 Vol.9 No.1

분말형 견운모는 폐기물 재활용 차원에서 화학, 광물 분야에서 활발하게 연구가 이루어지고 있고, 비교적 저렴하게 구할 수 있는 재료이며, 일반 운모와 마찬가지로 낮은 열전도율을 가지고 있다. 따라서 본 연구에서는 분말형 견운모를 활용하여 모르타르의 열전도율을 개선하고, 이를 통해 시멘트 복합체용 내화재료로서와 활용가능성을 확인하고자 하였다. 분말형 견운모를 혼입한 시멘트 모르타르를 제작하고, 이의 열전도율 및 고온 노출 전후의 압축강도를 비교 평가하여 내화성능을 규명하고자 하였다. 실험 결과에 따르면, 분말형 견운모를 혼입하는 경우 압축강도가 감소하는 것으로 나타났으며, 혼입량 증가에 따른 압축강도의 감소는 미미하였다. 분말형 견운모를 혼입하면, 열전도율이 감소하며, 600°C, 900°C, 1,200°C에 소성된 모르타르 시험체의 잔존강도가 플레인 모르타르에 비해 높게 나타났다. 실험 결과를 종합적으로 평가해 볼 때, 분말형 견운모는 시멘트 복합체용 내화 재료로서의 활용가능성이 존재한다고 결론지을 수 있다. Powder type sericite has been actively researched in the area of chemistry and mineralogy in terms of waste recycling. It is a material that can be obtained relatively inexpensively with a low thermal conductivity like general mica, so in order to improve the thermal conductivity of the mortar, powder type sericite was used in this work. Compressive strengths of mortar before and after high temperature exposure were compared and evaluated to determine the fire resistance of mortar with powder type sericite. According to the experimental results, it was found that the compressive strength decreased when powder type sericite was replaced with cement, but the decrease in compressive strength with the increasing amount of powder type sericite was insignificant. When powder type sericite was incorporated, the thermal conductivity decreased, and the residual strengths of the mortar specimens which were heat treated at 600°C, 900°C, and 1,200°C were higher than that of plain mortar. From the comprehensive evaluation of the experimental results, it can be concluded that the powder type sericite has the potential to be used as a refractory material for cement composites.

증례 : 감염 ; 불명열 에이즈 환자에서 발생한 파종성 조류형 결핵균 감염 1예

허현미 ( Hyun Mi Heo ),전윤희 ( Yoon Hee Jun ),김소이 ( So I Kim ),이명원 ( Myung Won Lee ),성순희 ( Sun Hee Sung ),이미애 ( Mi Ae Lee ),최희정 ( Hee Jung Choi ) 대한내과학회 2010 대한내과학회지 Vol.79 No.2

우리나라에서 에이즈환자의 기회감염증은 칸디다증, 결핵과 주폐포자충 폐렴이 가장 흔하며 그 중 결핵이 가장 위중한 기회감염이나, 최근에는 국내에서도 결핵 유병률의 감소와 더불어 비결핵 항산균 감염이 점차 증가하고 있다. 따라서 항산균 양성 환자에서 결핵과 비결핵 항산균의 감별이 중요하다. 최근에는 real-time PCR법이 개발되어 기존의 중합효소 연쇄 반응법보다 빠르 고 결핵균과 비결핵 항산균을 함께 검출하여 감별할 수 있다. 저자들은 에이즈 환자에서 복강내 임파절 조직검사와 real-time PCR법을 통해 확진된 파종성 결핵균 감염 환자를 화학 요법으로 치료하였기에 이를 보고하는 바이다. Since the first AIDS case was reported in 1985, the number of patients with AIDS and infectious complications has been increasing in Korea. Mycobacterium avium complex (MAC) infection is a common opportunistic infection in patients with AIDS, especially those with lower CD4+T cell counts. Although MAC is a common systemic bacterialinfection in developed countries, few cases have been reported among AIDS patients in Korea. MAC infection is rare in an immunocompetent person, but is an important cause of morbidity and mortality in patients with AIDS. MAC infection is typically associated with the degree of immunosuppression, duration of HIV infection, and low CD4+T cell counts. Here we report a case of disseminated MAC infection in an AIDS patient with fever, mesenteric lymph node enlargement, and pancytopenia. (Korean J Med 79:210-214, 2010)

원발성 복막수염의 임상양상

박재정 ( Jae Jung Park ),정성애 ( Sung Ae Jung ),노영욱 ( Young Wook Noh ),김고흔 ( Go Heun Kim ),허현미 ( Hyun Mi Heo ),배서은 ( Suh Eun Bae ),최윤정 ( Yun Jung Choi ),김소이 ( So I Kim ),이명원 ( Myung Won Lee ),강민정 ( Min Jun 대한장연구학회 2009 Intestinal Research Vol.7 No.1

Background/Aims: Primary epiploic appendagitis (PEA) is a rare cause of focal abdominal pain in otherwise healthy patients. Patients with acute abdominal pain are often misdiagnosed clinically as acute appendicitis or diverticulitis. The purpose of this study was to describe the clinical presentation and characteristic computed tomography (CT) findings of PEA. Methods: We reviewed the clinical records and CT images of 23 consecutive patients in Korea who presented with acute abdominal pain between January 2005 and February 2009 and had radiologic signs of PEA. Results: Twenty-three patients (7 females and 16 males; average age, 42±14 years) were diagnosed with symptomatic PEA. Abdominal pain localized to the left (8 patients [44.5%]) and right (10 patients [55.5%]) lower quadrants as the leading symptom. CT findings specific for PEA were present in all patients except one. The symptoms resolved within 1 week (mean, 3.5 days) with or without antibiotic treatment. Conclusions: In patients with localized, sharp, acute abdominal pain not associated with other symptoms, such as nausea, vomiting, fever or atypical laboratory values, the diagnosis of PEA should be considered and the diagnosis confirmed by CT scan. (Intest Res 2009;7:47-51)