Genistein이 햄스터 난소세포의 항산화효소활성과 발현에 미치는 영향

김민혜,김안근,Kim, Min-Hye,Kim, An-Keun 대한약학회 2007 약학회지 Vol.51 No.1

Reactive oxygen species (ROS) are produced in the metabolic process of oxygen in cells. The superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in cells systemize the antioxidant enzymes to control the oxidative stress. Genistein is one of the isoflavonoids, and its role in controlling cellular oxidative stress is presently the active issue at question. In this study; we analyzed genistein-induced survival rates of the CHO-K1 cells, activities of antioxidant enzymes, ROS levels, and expression levels of antioxidant enzyme genes in order to investigate the effect of genistein on cellular ROS production and antioxidative systems in CHO-K1 cells. As results, the survival rate of cells was decreased as the dose of genistein increases (12.5${\sim}$200 ${\mu}$M). Genistein increased cellular ROS levels, while it reduced total SOD activities and the expression of CuZnSOD. In conclusion, we suggest that genistein may induce oxidative stress via down-regulation of SOD.

햄스터 난소세포에서 Daidzein과 Genistein에 의해 유도된 산화적 스트레스에 대한 Vitamin C의 효과

김민혜,김안근,Kim, Min-Hye,Kim, An-Keun 대한약학회 2007 약학회지 Vol.51 No.4

The oxidative stress causes many diseases like cancer, aging, cardiovascular disease, degenerative neurological disorders (Parkinson’s disease, and Alzheimer's disease) by damage of cell membrane, protein deformation, and damage of DNA due to the oxidation of lipid of cell membrane, protein of tissue or enzyme, carbohydrate, and DNA. It is caused by the reactive oxygen species (ROS) that is produced in the metabolic process of oxygen in cell. The superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in cell systemize the antioxidative enzymes to control the oxidative stress. In this research, it is measured that the survival rate of cell by the typical isoflavonoid of daidzein or genistein, activity of antioxidative enzyme, and ROS level, in order to study the effect of isoflavonoid over the ROS production in cell and antioxidative system. As the similar action of the isoflavonoid with the estrogen is examined, women are encouraged to get bean. In view of this trend, it is very important to find out a combination medicine that lowers the oxidative stress caused by the daidzein in the ovarian cell. In the combined treatment of the typical antioxidant of vitamin C to oxidative stress which induced by daidzein recover the control level particularly lowering the ROS in cell by 30%. However, it made no effect in the combined treatment with genistein. Therefore, the research took the combination effect of daidzein with vitamin C in order to check it effect over the antioxidative system. In conclusion, it was disclosed that the oxidative stress caused by daidzein is related to the lowering activity of SOD, and the specific combination effect of daidzein with vitamin C is related to the recovery of SOD activity.

성인에서 기관지과민성과 폐기능의 지표로서 High-Sensitivity C-Reactive Protein의 의미

김민혜 ( Min Hye Kim ),김세훈 ( Sae Hoon Kim ),강혜련 ( Hye Ryun Kang ),박흥우 ( Heung Woo Park ),장윤석 ( Yoon Seok Chang ),김동희 ( Dong Hee Kim ),조상헌 ( Sang Heon Cho ),민경업 ( Kyung Up Min ),김유영 ( You Young Kim ),김선신 대한천식알레르기학회 2009 천식 및 알레르기 Vol.29 No.2

Background: Bronchial hyperresponsiveness is a characteristic feature of asthma and closely related with airway inflammation in part. Serum high-sensitivity C-reactive protein (hs-CRP) is a very sensitive marker for systemic inflammation, and several studies has reported the association between hs-CRP and bronchial hyperresponsiveness. Objective: The objectives of this study are to evaluate the association between hs-CRP and bronchial hyperresponsiveness or lung function in healthy Korean subjects. Method: A total of 7,709 subjects underwent methacholine bronchial provocation tests in Seoul National University Hospital health care Gangnam center from October 2003 to January 2009. Among them, 508 subjects were positive for the methacholine bronchial provocationtest. Another 508 subjects negative for the test were enrolled as control, who were matched for age, sex and body mass index. We reviewed the medical records and compared serum hs-CRP levels and the results of the methacholine bronchial provocation test and pulmonary function tests. Result: Bronchial hyperresponsiveness was more frequently observed in subjects with higher hs-CRP levels (group IV). (24.6% vs. 14.8%) than in subjects with lower hs-CRP levels (group I). There was a negative correlation with hs-CRP levels and FEV1, FVC or FEV1/FVC. Conclusion: There was a negative association between serum hs-CRP levels and bronchial hyperresponsiveness or lung function. (Korean J Asthma Allergy Clin Immunol 2009;29:112-116)

보문 : 옵아트(Op-Art) 패턴의 여행용 패션가방 디자인 연구 -빅토르 바자렐리(Victor Vasarely)의 작품 응용을 중심으로-

김민혜 ( Min Hye Kim ),박혜원 ( Hye Won Park ) 한국의류학회 2011 한국의류학회지 Vol.35 No.3

This study with ``trip`` as a theme, aims to develop textile designs and fashion bags for travel which is widely applicable fitting traveler`s T·P·O, applying Victor Vasarely`s artwork. During the planning process of design, ``Whenever & Wherever``s concept was set by analyzing fashion trend information in 2010-11 F/W proposed by Interfashion Planning and FCK; in addition, ``Bobos`` were selected as research targets. It organized 4 images of ``Modern``, ``Romantic``, ``Classic`` and ``Fantastic``. The motives are proposed textile design with ``Cross 2``, ``Falling Z``, ``Cir-Classicism`` and ``Skinny-holic`` per each theme. The designs developed were printed out and into polyester canvas with a Polaris V6 DTP from dgen, Corp. Textile designs developed were applied for ``two-way`` formed fashion bags for traveling with high practicality. Fashion bags for traveling consisted of big bags, tote bags, and hip sacks; a total of 12 works (4 sets per theme) were produced. This study sought practical plans for artworks by producing fashion goods through a DTP system. The results of this study can be used as guidelines for further studies and as a significant contribution to the creation of high values for exciting fashion products.

디카르복실 산의 염료감응형 태양전지의 효율 향상 효과

김민혜 ( Min Hye Kim ),권영욱 ( Young Uk Kwon ) 한국공업화학회 2006 응용화학 Vol.10 No.1

시민참여가 삶의 질에 미치는 영향에 관한 연구 : 정부신뢰의 조절효과를 중심으로

김민혜 ( Kim Min Hye ),이승종 ( Lee Seung Jong ) 한국행정연구원 2019 韓國行政硏究 Vol.28 No.4

본 연구는 대부분의 삶의 질 지수조사에서 주관적 삶의 질 영역의 주요 지표로 측정하는 시민참여가 실제로 삶의 질의 영향요인으로 작용하고 있는지 확인하고자 하였다. 이를 위해 선거참여를 정치참여수단으로, 정부의 정책과정이나 활동에 영향력을 주고자 행동하는 시민의 활동을 행정적 참여인 시민관여로 구분하여 설정하고, 각각의 참여수단이 주관적 삶의 질에 미치는 영향을 분석하였다. 분석 결과, 선거참여는 삶의 질에 긍정적 영향을 미치는 것을 확인할 수 있었지만, 정부신뢰는 삶의 질에 통계적으로 유의미한 결과를 가져오지 않았다. 반면 행정적 참여수단인 시민관여는 직접적으로는 삶의 질에 유의미한 효과가 없는 것으로 나타났지만, 정부신뢰의 효과를 고려했을 때는 그 결과가 달라졌음을 알 수 있었다. 왜냐하면 시민의 정부신뢰 수준에 따라 참여가 삶의 질에 미치는 효과의 방향성이 다르게 나타났기 때문이다. 고수준의 정부신뢰를 가진 시민들은 참여할수록 삶의 질이 높아진다고 인식하는 반면, 저수준의 정부신뢰를 가진 시민은 아무리 정책과정에 참여하고 시정요구를 해도 그러한 활동에서 오는 정부의 피드백이 삶의 질을 높였다고 보지 않는다는 것을 확인할 수 있다. The purpose of this study was to determine whether citizen participation, which is a key indicator of subjective quality of life, is an important factor in improving quality of life. For this, the type of citizen participation is divided into presidential election turnout and citizen involvement(administrative participation), which are used in measuring the quality of life indicators, and then the effect of each means of participation on subjective quality of life is analyzed. The results show that turnout has a positive effect on the quality of life, but government trust has no statistically significant effect on the quality of life. On the other hand, citizen involvement did not directly have a significant effect on quality of life, but the results were different when the effect of trust in government was taken into account. This is because the degree of citizen involvement has a different effect on the quality of life, depending on the level of trust in government. Citizen participation with high levels of government trust has had a positive impact on quality of life while citizens with low government trust did not regard the government's feedback from such activities as enhancing the quality of life, no matter how much they participated in the policy process or the corrective request.

현대 디지털 전시공간에 나타난 뉴미디어 표현특성에 관한 연구

김민혜(Kim, Min-Hye),김문덕(Kim, Moon-Duck) 한국실내디자인학회 2007 한국실내디자인학회 학술대회논문집 Vol.9 No.3

부모의 과보호적 양육태도가 대학생의 학업소진에 미치는 영향: 자아탄력성과 친구관계 질의 조절된 매개효과

김민혜(Kim Min-Hye),오인수(Oh In-Soo) 부산대학교 교육발전연구소 2019 교육혁신연구 Vol.29 No.1

연구목적: 본 연구는 부모의 과보호가 대학생 자녀의 학업소진에 미치는 영향에 있어 자아탄력성과 친구관계 질의 조절된 매개효과를 확인하고자 하였다. 연구방법: 대학생 278명을 대상으로 온라인 설문조사를 실시하였다. 최종 선정된 276개의 자료는 SPSS 프로그램과 Process macro를 사용하여 분석하였다. 연구결과: 첫째, 과보호적 양육태도는 학업소진에 직접적인 영향을 미치는 것으로 나타났다. 둘째, 자아탄력성은 과보호적 양육태도와 학업소진의 관계를 부분매개하는 것으로 나타났다. 셋째, 부모의 과보호적 양육태도가 자아탄력성을 통해 학업소진에 미치는 간접적인 영향은 친구관계 질에 의해 조절되는 것으로 나타났다. 논의 및 결론: 본 연구는 부모의 과보호적 양육태도, 자아탄력성, 친구관계 질이 대학생의 학업소진에 영향을 미침을 확인하였다. 이러한 연구결과를 바탕으로 대학생의 학업소진을 줄이기 위한 교육상담적 지원이 필요함을 제안하였다. Purpose: The purpose of this study was to identify the relationship between parental overprotection and college student burnout, focusing on the moderated mediating effect of ego-resiliency and friendship quality. Method: An online survey was conducted on 278 college students. A total of 276 data were analyzed by the 3-step moderated mediation analysis proposed by Preacher, Rucker, and Hayes(2007). Results: The results of this study were as follows: First, parental overprotection had a significant direct effect on college student burnout. Second, ego-resiliency partially mediated the relationship between parental overprotection and college student burnout. Third, friendship quality moderated the mediating effect of ego-resiliency in the relationship between parental overprotection and college student burnout. Conclusion: This study showed that parental overprotection, ego-resiliency and friendship quality have influences on college student burnout. Based on the results, educational counseling and related suggestions were proposed to reduce college student burnout.

인슐린 알레르기 환자에서 탈감작 후 나타난 인슐린 특이항체의 변화

김민혜 ( Min Hye Kim ),임경환 ( Kyung Hwan Lim ),박한기 ( Han Ki Park ),김병근 ( Byung Keun Kim ),강민구 ( Min Koo Kang ),권재우 ( Jae Woo Kwon ),김태완 ( Tae Whan Kim ),정재우 ( Jae Woo Jung ),이소희 ( So Hee Lee ),이상민 ( Sang 대한천식알레르기학회 2010 천식 및 알레르기 Vol.30 No.1

Although human insulin is widely used, insulin allergy is still an important issue. Desensitization is one of the strategies to overcome insulin allergy, but there are few report concerning the mechanism of immune tolerance to insulin achieved by desensitization. Based on the previous studies regarding immunotherapy, it can be understood that changes in allergen specific antibody may be a probable mechanism. We report time-serial changes in insulin-specific antibodies after successful desensitization of type I hypersensitivity to insulin. A 68-year-old man visited our clinic due to dyspnea, loss of consciousness and whole body rash with erythema immediately after the injection of human insulin. The patients showed positive skin reactions to all available human insulins. Enzyme linked immunosorbent assay (ELISA) demonstrated that human insulin specific IgE level increased. We injected human insulin (aspart) to patient subcutaneously with gradual increase manners and finally achieved successful insulin desensitization without serious systemic reactions. Human insulin-specific antibody was measured 4, 8 and 12 weeks after desensitization, which showed interesting time-serial changes; specific IgE gradually decreased, while IgG1, IgG2 and IgG4 gradually increased. (Korean J Asthma Allergy Clin Immunol 2010; 30:63-67)

지연형 조영제 과민반응의 임상 양상

김민혜 ( Min Hye Kim ),이서영 ( Suh Young Lee ),이승은 ( Seung Eun Lee ),김미영 ( Mi Yeong Kim ),조은정 ( Eun Jung Jo ),박창민 ( Chang Min Park ),이활 ( Whal Lee ),조상헌 ( Sang Heon Cho ),강혜련 ( Hye Ryun Kang ) 대한천식알레르기학회 2014 Allergy Asthma & Respiratory Disease Vol.2 No.5

Purpose: Delayed hypersensitivity reaction can occur in a couple of hours to several days after injection of iodine-based contrast media (ICM). ICM-related delayed type hypersensitivity is not common but increasing as rapid growth of ICM use. Nevertheless, objective data on delayed type hypersensitivity are still scarce worldwide including Korea. This study was performed to investigate the clinical features of ICM-induced delayed hypersensitivity in Korean patients. Methods: We retrospectively reviewed the electronic medical records of patients diagnosed with delayed hypersensitivity to ICM from January 2009 to December 2012 at Seoul National University Hospital and analyzed the data to identify the clinical characteristics of these patients. Results: A total of 44 cases were diagnosed as delayed-type hypersensitivity to ICM. The mean age was 54 years, and 70.5% were female. The mean number of previous ICM exposure was 3.8, and skin reactions were the most common symptoms. In 45% of patients, hypersensitivity reaction developed on the first exposure to ICM. Among the 27 patients exposed to ICM again, hypersensitivity reactions recurred in only 4 patients (14.8%). There was no difference of recurrence rate according to the use of premedication or the change in ICM. Conclusion: In this study, we observed a female predominance and a low recurrence rate in delayed hypersensitivity to ICM. Premedication and ICM change was not effective in preventing recurrence of delayed type reactions.(Allergy Asthma Respir Dis 2014;2:352-357)