근적외선 유도 약물방출을 위한 키토산 미세입자의 제조와 평가

김민아 ( Min Ah Kim ),김미리 ( Miri Kim ),이창문 ( Chang-moon Lee ) 한국키틴키토산학회 2016 한국키틴키토산학회지 Vol.21 No.4

본 연구에서는 근적외선 레이저의 감응성 물질인 멜라닌과 5-FU를 함유한 키토산 미세입자를 제조하고 광열효과와 약물방출실험을 진행하고 다음과 같은 결과를 얻었다. 멜라닌과 5-FU를 함유한 키토산 미세입자는 W/O/W 방법을 통해 성공적으로 제조할 수 있었고, 미세입자는 구형이었으며 비교적 균일하였다. 키토산 미세입자를 농도별로(0.55~2.75 mg/mL) 증류수에 분산시켜 레이저(808 nm, 1.5 W/cm², 5 min)를 조사한 결과, 샘플의 농도와 조사시간이 증가할수록 온도의 변화가 크게 나타났다. 키토산 미세입자로부터 5-FU의 방출실험을 진행한 결과, 레이저를 조사하지 않은 그룹에 비해 레이저를 조사한 그룹에서 약물방출 속도가 증가하는 것을 알 수 있었고, pH 5.0에서 보다 큰 차이를 보였다. 위의 결과를 통해 근적외선을 이용하여 멜라닌을 함유한 키토산 미세입자로부터 약물 방출 속도를 조절할 수 있고 더 나아가 in vivo에서의 국소적 약물 전달 및 효과적인 치료방법으로 사용될 수 있을 것으로 기대할 수 있다. In this study, melanin and 5-fluorouracil (5-FU)-loaded chitosan microparticles were prepared and the drug release profiles from the microparticles were investigated under near infrared (NIR) 808 nm laser irradiation at 1.5 W/cm ² for 5 min. The chitosan microparticles were uniform and spherical in shape. After irradiation of NIR 808 nm laser at 1.5 W/cm² for 5 min, the temperature of the chitosan microparticle solution increased. As the concentration of the chitosan microparticles increases from 0.55 mg/mL to 2.75 mg/mL, the temperature of the microparticle solution increased with irradiation time. Under NIR 808 nm laser irradiation, 32.1±2.3% and 53.7±1.1% of 5-FU from the chitosan microparticles were released at pH 7.4 and 5.0, respectively. In other hand, without laser irradiation 23.9±1.6% and 26.9±2.0% of 5-FU were released from the chitosan microparticles at pH 7.4 and 5.0, respectively. All these results indicate that chitosan microparticles containing melanin could be used as a photo-induced drug release system.

융합인재교육(STEAM)에 대한 유아교사의 인식 및 활성화 방안

김민아,김승희,Kim, Min-Ah,Kim, Seung-Hee 한국보육지원학회 2017 한국보육지원학회지 Vol.13 No.5

Objective: This study aims to investigate early childhood teachers' perception of STEAM and suggest activation measures for STEAM according to teachers' background variables. Methods: The researchers conducted a pilot study, and then did survey research for 5 weeks. The participants of this study included 523 teachers of early childhood education institutions in Gwangju metropolitan city and Jeollanam-do Province. Results: The results of this study were as follows. First, early childhood teachers perceived the necessity of STEAM differently according to their age, experience, and academic background. The higher their age, experience, and academic background were, the more they wanted to participate in further teacher training and education. In particular, early childhood teachers' perception and activation measures were very different according to their workplace. These results come from the fact that the conditions in conducting STEAM are different according to their workplace. Conclusion/Implications: In order to activate STEAM, a teacher training program including practical contents and methods should be conducted. Also, it is necessary to carefully organize the content and structure of the teacher training program according to their workplace. This study is meaningful in terms of suggesting activation measures for STEAM by showing how early childhood teachers think about STEAM and what they demand.

DNA Sequence Visualization with k-convex Hull

김민아,이은정,조환규,Kim, Min Ah,Lee, Eun Jeong,Cho, Hwan Gyu Korea Computer Graphics Society 1996 컴퓨터그래픽스학회논문지 Vol.2 No.2

In this paper we propose a new visualization technique to characterize qualitative information of a large DNA sequence. While a long DNA sequence has huge information, it is not easy to obtain genetic information from the DNA sequence. We transform DNA sequences into a polygon to compute their homology in image domain rather than text domain. Our program visualizes DNA sequences with colored random walk plots and simplify them k-convex hulls. A random walk plot represents DNA sequence as a curve in a plane. A k-convex hull simplifies a random work plot by removing some parts of its insignificant information. This technique gives a biologist an insight to detect and classify DNA sequences with easy. Experiments with real genome data proves our approach gives a good visual forms for long DNA sequences for homology analysis. 본 논문에서는 대용량의 DNA 염기 배열의 정성 정보를 특징짓기 위한 새로운 가시화 방법을 제안한다. DNA 배열은 배열 자체가 방대한 양의 정보를 포함하고 있기 때문에 분석에 많은 어려움이 있다. 우리는 DNA 염기 배열들사이의 상사성 비교를 위해 DNA 염기 배열을 하나의 이미지 도메인으로 변환한다. 프로그램은 random walk plot으로 DNA 염기 배열을 가시화한 후에 k-convex hull로 단순화 시킨다. Random Walk plot은 염기배열을 평면상에 하나의 커브로 표현한다. k-convex hull은 walk plot으로부터 무의미한 부분을 제거함으로서 walk plot을 단순화한다. 이러한 방법은 유전공학자들에게 쉽게 DNA 배열의 특징을 인식하고 분류할 수 있는 직관을 제공한다. 실제 게놈 데이터로 실험한 결과는 논문에서 제안하는 방법이 긴 DNA 염기배열들 사이의 유사성 분석을 위해 좋은 가시화 도구임을 보여준다.

교통사고후유증(交通事故後遺症)(편타성(偏墮性) 손상(損傷))에 대한 침구치료(鍼灸治療)의 접근(接近)

김민아,박용진,Kim, Min-Ah,Park, Yong-Jin 대한침구의학회 2000 대한침구의학회지 Vol.17 No.3

Objectives : This study is to applied effectively to the acupuncture treatment for disorders following whiplash injuries. Methods : This study suggests five various ways and principtes of treating disorders following whiplash injuries by means of acupuncture. First principle is that acupuncture treatment is carried out on acupoint far from cervical spine at acute stage, on the other hand, Extraordinary Vessel and other acupuncture treatment such as five element acupuncture treatment is applied at chronic stage in order to control balance disoders due to cervical pathology. Second is acupuncture treatment will be performed in group of three part of Meridian System-Yangmyung, Taeyang, Soyang-according to cervical kinetic, its characteristic and location of pain. Third is acupuncture treatment could be performed on basis of theory of Eohyel-disorders and disturbance of blood circulation. Fourth is to decrease tension of SCM muscle, because disorders following whiplash injuries has much association to injury of CM muscle and is simillar to tension syndroms of SCM muscle. fifth is taping therapy could control and decrease disorders following whiplash injuries based on spiral balance taping theory. Conclusions : These results suggest that acupuncture treatment for disorders following whiplash injuries will significally decrease pain and control syndrom caused by cervical disorder.

근적외선 레이저 반응성 광열-기포 생성 알지네이트 나노입자로부터 제어된 약물 방출

김현지 ( Hyunji Kim ),김민아 ( Min Ah Kim ),이창문 ( Chang-moon Lee ) 한국키틴키토산학회 2021 한국키틴키토산학회지 Vol.26 No.3

본 연구에서는 근적외선에 반응하는 알지네이트 기반 나노복합체를 제조하여 광열효과 및 버블생성으로 인한 약물 방출을 효과적으로 조절하고자 하였다. 제조된 AMP NPs의 평균 크기는 252.52 ± 0.39 nm로 측정되었고 구형의 형태를 나타내었다. 수용액 내에서의 침전이나 응집이 관찰되지 않았으며 7일 동안 사이즈의 큰 변화가 관찰되지 않았다. AMP NPs에 808 nm 레이저 조사 시 조사 시간 및 레이저 세기가 증가할수록 효과적인 온도 상승을 보였다. 생성된 열로 인해 PFH의 상전이를 유도하여 이에 따른 AMP NPs의 극적인 버블 생성을 초음파 영상으로 확인하였다. 약물 방출 평가에서 AMP NPs에 레이저 조사 시 약물 방출 속도를 향상시켰으며 반복적인 레이저 조사 시 순간적으로 약물의 방출이 증가하였다. 이러한 결과로부터, AMP NPs는 근적외선 반응성 약물 방출 조절제제로서의 가능성을 확인하였고, 이는 병변에서 테라노스틱 제제로 활용 될 수 있을 것으로 기대된다. In order to maximize the therapeutic effect of a drug, it is necessary to control the release rate of the drug or efficiently deliver it to an accurate target site. In this study, alginate-melanin-methotrexate-perfluorohexane nanoparticles (AMP NPs) were proposed for controlling the drug release by near-infrared (NIR) laser-responsive photothermalbubbling effect. AMP NPs had a uniform spherical shape with a size of 252.52 ± 0.39 nm and did not show precipitation in PBS buffer for 7 days. Based on the unique light-to-heat conversion properties of melanin, the temperature of AMP NPs rose to about 52.9℃ after irradiation with an 808 nm NIR laser with an intensity of 1.5 W/㎠ for 10 min. As the exotherm of melanin reached the phase transition temperature of perfluorohexane (PFH), microbubbles from the AMP NPs solution were generated dramatically. The release rate of methotrexate from AMP NPs increased to 61% after 24 h of laser irradiation due to the heat generation of melanin and bubble generation of PFH in AMP NPs. From these results, it is expected that AMP NPs can be utilized as promising carriers for controlled drug release.

『법화경』의 비유 연구 - 예수 비유와의 비교를 중심으로 -

김민아 ( Kim Min-ah ) 서울대학교 종교학연구회 2016 宗敎學硏究 Vol.34 No.-

Religious truths are so intricate and profound that it`s hard for ordinary people to realize them. The parables are used to help ordinary people understand the truth. That is why religious texts include a wide variety of parables. Lotus Sutra also has abundant parables. This article aims to study the characteristics of the parables in the Lotus Sutra. First, Buddha`s parables in the Lotus Sutra explain how the Buddha teaches people via a means. Second, the parables in the Lotus Sutra are directed towards a comprehensive range of people regardless of age, sex, social class, or education. Third, Buddha`s parables are the natural narratives with no surprise, unpredictability, and irony.

게쉬탈트 심리학에 의한 아이콘의 인지학적 디자인 형태지각에 관한 연구

김민아(Min Ah Kim) 한국디자인문화학회 2007 한국디자인문화학회지 Vol.13 No.4

한국 복음주의 사회운동의 분화와 개신교 뉴라이트의 등장

김민아(Kim Min-Ah) 한국기독교역사연구소 2018 한국기독교와 역사 Vol.- No.48

1980년대 등장한 복음주의 사회운동은 민중운동 및 민주화운동과의 거리두기를 통해 자신의 정체성을 확립하고자 하였고, 복음주의 내에서 급진적인 분파와의 갈등을 통해 더욱 온건해지는 경향을 보여주었다. 이 ‘온건한 복음주의 사회운동’은 마르크스주의와 민중신학, 폭력투쟁이라는 운동 전략, 그리고 사회변혁이라는 운동 목표로부터 거리를 두고, 개인적인 각성을 통한 점진적인 사회변화를 목표로 하였다. ‘온건한 복음주의 사회운동’은 ‘개신교 뉴라이트’의 ‘정치적 행동주의’ 형성에 영향을 끼쳤다. 개신교인의 사회적 책임을 적극적으로 인정하는 ‘로잔 언약’이 ‘개신교 뉴라이트’의 정치참여를 정당화해 주는 근거로 활용된 것이다. 한편 해방 이후부터 이어져오고 있는 ‘국가조찬기도회’의 ‘민족복음화’라는 정치적 전망은 ‘개신교 뉴라이트’ 운동의 정치적 지향 설정에 영향을 끼쳤다. 이렇게 형성된 ‘개신교 뉴라이트’의 종교 · 정치적 성향은 한마디로 ‘보수 정치적 행동주의’라고 할 수 있다. 이처럼 이명박 · 박근혜 정권을 탄생시킨 ‘개신교 뉴라이트’는 기존의 정통적 보수적인 개신교인들과는 다른 종교·정치적 성격을 가진, 새롭게 등장한 개신교 정치세력이다. The moderate evangelical social movement emerged in the 1980s, differentiating itself from the Minjung movement and the democratization movement. It tended to become even more moderate by going through conflicts with a radical evangelical social movement. This moderate evangelical social movement aimed at gradual social changes through individual awakening, distancing itself from Marxism, Minjung Theology, violent protests, and social revolutions. The moderate evangelical social movement influenced the formation of political activism by the Christian New Right. The Lausanne Covenant that vocally espoused the Christian’s social responsibility justified political participations of the Christian New Right. Meanwhile, the idea of national evangelization-which was a political goal of the National Prayer Breakfast- had an impact on the political orientation of the Christian New Right. The religious and political orientation of the Christian New Right can be summarized as ‘conservative political activism.’ The Christian New Right- which contributed to the creation of Lee Myeong-bak’s and Park Geun-hye’s administrations- was a newly emerging Christian political power group, different from the existing old conservative Christians.

광역학 세포 사멸을 위한 키토산-피코시아닌 나노입자

김민아 ( Min Ah Kim ),이창문 ( Chang-moon Lee ) 한국키틴키토산학회 2020 한국키틴키토산학회지 Vol.25 No.4

본 연구에서는 감광제인 피코시아닌을 키토산과 제형화시킴으로써 종양세포에 대한 선택적인 축적을 유도하여 광역학 치료 효율을 향상시키고자 하였다. 키토산-피코시아닌 나노입자의 평균 크기는 308.02±3.29 nm로 측정되었고 구형의 형태를 보였다. 키토산과 피코시아닌의 결합 유무를 FT-IR 분석을 통해 평가한 결과, 성공적으로 이온복합체를 형성하였음을 확인하였다. DPBF 평가에서 키토산-피코시아닌 나노입자는 레이저 조사 하에 활성산소종을 생성하였고 입자 형성 후에도 피코시아닌의 광역학 활성이 유지됨을 확인하였다. 키토산-피코시아닌 나노입자 단독으로는 세포 독성이 관찰되지 않았고, 반면에 레이저를 조사 시 대장암 세포에서의 광역학 치료 매개 세포 사멸을 유도하였다. 이러한 결과로부터, 키토산-피코시아닌 나노입자는 광역학 치료를 위한 감광제로서의 가능성을 확인하였고, 암 치료뿐만 아니라 다양한 병변에서도 활용될 수 있을 것으로 기대된다. C-phycocyanin (C-PC), a natural biomaterial, is attracting attention for its unique photodynamic activity, antiinflammatory and anti-cancer properties. To solve the problem of systemic distribution of photosensitizers, a nanoformulation strategy is essential. The aim of this study is to prepare chitosan-phycocyanin nanoparticles and evaluate their photodynamic therapeutic activity to confirm their potential as a photosensitizer. The average size of chitosanphycocyanin nanoparticles was measured to be 308.02±3.29 nm and showed a spherical shape. In the DPBF evaluation, it was confirmed that the chitosan-phycocyanin nanoparticles generated reactive oxygen species (ROS) under near infrared (NIR) laser irradiation, and the photodynamic activity of phycocyanin was maintained even after particle formation. Although no cytotoxicity was observed with chitosan-phycocyanin nanoparticles, it induced photodynamic therapymediated apoptosis in CT26 cells under NIR laser irradiation. All these results indicate that chitosan-phycocyanin nanoparticles could be attractive therapeutics for photodynamic therapy.

공간구문론을 이용한 서울과 평양의 도시공간배치 특성 비교분석

김민아(Kim Min Ah),이태호(Lee Tae Ho),반영운(Ban Yong Un) 한국지역개발학회 2011 한국지역개발학회 학술대회 Vol.2011 No.6

This study has intended to verify what of logic Seoul, the capital of the Republic of Korea, and Pyongyang, the capital of the Republic of the Joseon People's Democracy, which are divided due to ideological differences, was changed through, to intend to compare and contrast the ways in which the city environments have changed and to determine the varying characteristics of both city spaces in order to present a theoretical basis for the incoming unification. Furthermore, this study employed a space syntax analysis through axial maps of 1930s and 2000s for the analysis of spatial configurations before and after the systems's changes. As a result, we found that two cities have shown a different land-use pattern and landscape at the core of the cities according to ideological differences between them. In addition to, some representative indicator values of space syntax such as 'integration', 'connectivity' have changed, in particular, in case of core analysis of two cities, the result has shown any different changing pattern of the cities under the ideological differences.