http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
김대병,이종권,정경자,윤여표,김명수,Kim, Dai-Byung,Lee, Jong-Kwon,Jung, Kyong-Ja,Yoon, Yeo-Pyo,Kim, Myung-Soo 한국독성학회 1998 Toxicological Research Vol.14 No.3
Ingredients and concentrations of organic solvents in 7 kinds of adhesive sold on the domestic market were qualitatively and quantitatively analysed. Vapor concentrations were also analysed to estimate inhalation concentratins when adhesives were abused to get high. Acetone, methyl ethyl ketone, methyl cyclopenatane, cyclohexane and toluene were identified to be used in domstic adhesives as solvents. When organic solvents of adhesives were vaporized in the vial at the room temprature for 30 mins, concentrations of organic solvents in air were in the range of 5,000~140,000ppm. Among these solvents, toluene, known to have strong hallucination effect, showed 5,000~35,000 ppm. The putative concentration of toluene in case of glue sniffing was estimated to be about 5,000ppm in consideration of glue sniffing circumstances. Toluene was found in all adhesives in this study, even adhesives which toluene was not described in label.
소염진통제 약물에 대한 In vitro 피부자극 시험연구
이종권,김대병,이은희,이선희,류승렬,최기환,김윤정,김부영,Lee, Jong-Kwon,Kim, Dai-Byung,Lee, Eun-Hee,Lee, Sun-Hee,Ryu, Seung-Rel,Choi, Ki-Hwan,Kim, Yoon-Jeong,Kim, Pu-Young 한국독성학회 1998 Toxicological Research Vol.14 No.3
In vitro skin iritation of anti-inflammatory drugs was investigated in terms of the cytotoxicity method to human skin fibroblast cells. Five anti-inflammatory drugs (Diclofenac, Naproxen, Meclofenamic acid, Ibuprofen and Fnoprofen) which are commercially available as oral preparations or injections were tested. The cytotoxicity of 5 chemicals was evaluated by using MTT[tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. NRU (neutral red uptake) assay and Alamar Blue assay after fibroblast cells had been exposed to the chemicals for 24 hours or 489 hours. The $IC_{50}$ values of the chemicals showed the comparative strength of cytotoxicity as following order of Meclofenamic acid>Diclofenac>Fenoprofen>Ibuprofen>Naproxen. The values of $IC_{50}$ determined by Alamar Blue assay were lower than those of MTT and NRU assay. These data suggest Alamar Blue assay can be useful method for assessing in vitro skin irritation potential of anti-inflammatory drugs.
잔류농약 24성분의 다성분 동시분석을 위한 기체크로마토그래피 조건의 최적화
이은주(Eun-Ju Lee),김우성(Woo-Seong Kim),박건상(Kun-Sang Park),오재호(Jae-Ho Oh),김대병(Dai-Byung Kim) 한국농약과학회 2000 농약과학회지 Vol.4 No.2
Optimum parameters were investigated for the simultanious analysis of 24 pesticide residuces using gas-liquid chromatography with electron capture detection. Electronic pressure control(EPC) on column enhanced resolution of 24 analytes. Using DB-17, SPB-608, and Ultra-2 capillary column without EPC, incomplete separation was observed in some pairs of pesticides. When EPC function was adopted, no severe overlapping was observed on SPB-608 column in every pesticides except vincloxolin/acetochlor pair. Total running time was 45 min, much shorter than 69~81 min when used without EPC. Limit of determination of each analyte ranged 0.1-12.9 ng/mL.
HPLC-FLD 및 LC-MS/MS에 의한 두류 중 제랄레논 오염실태 조사
장미란(Mi-Ran Jang),이창희(Chang-Hee Lee),이효정(Hyo-Jung Lee),김지연(Ji-Yeon Kim),손상혁(Sang-Hyuk Son),신춘식(Choon-Shik Shin),김소희(So-Hee Kim),김대병(Dai-Byung Kim) 한국식품과학회 2008 한국식품과학회지 Vol.40 No.3
국내유통 중인 대두, 붉은콩, 검은콩 녹두 등 6품목의 두류 총 127건을 immunoaffinity column 정제방법 및 HPLC-FLD을 이용하여 제랄레논에 대한 오염실태를 조사하였다. 상관계수(R²) 0.999이상으로 양호한 직선성을 보였고 검출한계 및 정량한계는 각각 2.0 ㎍/㎏, 6.0 ㎍/㎏, 회수율 82.2-98.4%로 나타났다. RSD가 0.82-6.40%로 양호한 재현성을 나타내었다. 모니터링 결과, 대두(백태) 27건 중 1건(3.7%)에서 37.62 ㎍/㎏ 검출되었고 붉은콩(팥) 27건 중 12건 검출되어 44.4%의 검출율을 보였고 그 오염수준은 8.01-38.98 ㎍/㎏이었다. 나머지 시료 검정콩 16건, 녹두 24건, 서리태(속청) 19건, 서목태(약콩) 14건에서는 모두 검출되지 않았다. 따라서 총 시료 127건 중 13건 검출되어 10.2%의 검출율을 보였고 그 오염수준은 8.01-38.98 ㎍/㎏이었다, 검출시료 13건을 LCMS/MS로 확인한 결과 모두 제랄레논임이 확인되었다. 본 연구결과에서 나타난 두류의 검출빈도 및 오염수준은 유럽연합에서 설정된 기준규격 이하의 수준이었으나 곰팡이독소의 생성의 특이성을 고려한다면 지속적이고 광범위하게 오염실태 조사가 되어야 한다고 판단된다. A survey for zearalenone contamination was conducted on 27 soy bean samples, 27 red bean samples, 16 black bean samples, 19 seoritae samples, 14 seomoktae samples, for a total of 127 commercial Korean samples. Zearalenone was quantified by the immunoaffinity column clean-up method with high performance liquid chromatography-fluorescence detection (HPLC-FLD), and was confirmed by liquid chromatography tandem mass spectrometry(LC-MS/MS). The limits of detection and quantification were 2.0 ㎍/㎏ and 6.0 ㎍/㎏, respectively. The recovery in the beans ranged from 82.2 to 98.4%. According to HPLC-FLD, zearalenone was detected in 13 samples (10.2% incidence), including 1 soybean and 12 red bean samples. The zearalenone contamination levels were in the range of 8.01~38.98 ㎍/㎏. Finally, LC-MS/MS analysis was conducted in the contaminated samples to verify the results of HPLC-FLD. The LC-MS/MS results confirmed the presence of zearalenone in all 13 samples. The contamination level was lower than that of EU, which is below 100 ㎍/㎏ for raw grains.
최윤주(Youn Ju Choi),장재희(Jae Hee Jang),박혜경(Hye Kyung Park),박건상(Kun Sang Park),구용의(Yong Eui Koo),황인경(In Kyeong Hwang),김대병(Dai Byung Kim) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.2
기존의 미생물학적 분석법의 많은 단점을 보완하고자 식품중 판토텐산의 HPLC 분석법을 시도하였다. 추출용매는 20 mM potassium phosphate를 사용하였고, PDA spectrum 결과 최대 흡광도를 200 ㎚에서 분석하였다. HPLC 방법에 의한 판토텐산의 평균 회수율은 83.5~109.6%이었으며 검출 한계는 0.5 ppm이었다. 또한 HPLC 분석법의 신뢰성을 검증하고자 미생물학적 분석법도 병행했는데 그 결과 회수율은 87.0~118.3%이었고 검출한계는 0.000375 ppm으로서 미생물학적 분석법이 검출한계는 훨씬 낮았다. HPLC법이나 미생물학적 분석법(MBA)에서 대상식품중 판토텐산의 측정값은 13건의 시료에서 모두 표시값보다 높았다. 미생물학적 분석법(MBA)에 대한 HPLC 분석 회수율은 91.9~117.6%이었고, paired t-test 및 회귀분석결과, 두 방법 사이에는 유의적인 차이(p<0.01)가 없었으며, 상관관계(r = 0.9842, y =1.1428x-0.2269)가 양호하였다. 본 연구에 의해 개발된 HPLC 분석법은 기존의 미생물학적 분석법에 비하여 간단하면서 정확하여 분석의 효율성을 증대시킬 수 있으리라 기대된다. This study was conducted to develop an HPLC method for determining pantothenic acid in fortified foods which has typically been determined by microbiological assay (MBA) according to AOAC and Korean Food Code approved methods. Pantothenic acid was determined by reversed-phase ion-pair HPLC using UV absorption (200 ㎚) after extraction with 20 mM potassium phosphate solution by sonication. The recovery of spiked samples and detection limit (LOD) by HPLC were 83.5~109.6% and 0.5 ppm (㎎/㎏), respectively. The LOD of the microbiological assay (MBA) was much lower than that of HPLC. The concentrations of pantothenic acid analyzed in all tested samples (n=13) confirmed compliance with declared label claims. The range of recovery ratio by the HPLC method when compared to the microbiological assay was 91.9~117.6%. There was not significant difference (p<0.01) between the HPLC and MBA methods and the equation of the regression curve was y=1.1428x-0.2269 (r=0.9842). This proposed HPLC method for determining pantothenic acid appears to be suitable for determining pantothenic acid concentrations above 0.25 ㎎/100 g in fortified foods.
HPLC-FLD 및 LC-MS/MS에 의한 식품 중 총아플라톡신 오염실태 조사
장미란(Mi-Ran Jang),이창희(Chang-Hee Lee),조성혜(Sung-Hye Cho),박준식(Joon-Shik Park),권은영(Eun-Young Kwon),이은진(Eun-Jin Lee),김소희(So-Hee Kim),김대병(Dai-Byung Kim) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.5
국내유통중인 곡류, 견과류 및 그 가공품 총 25품목, 393건의 시료에 대해 immunoaffinity column 정제방법을 이용하여 총아플라톡신 오염실태를 조사하였으며, 그 결과 곡류 및 곡류가공품 6건, 견과류 및 견과류 가공품 37건에서 아플라톡신 오염이 확인되었으며 오염수준은 아플라톡신 B1으로서 0.04-2.65 μg/kg, 총아플라톡신으로서 0.04-5.51 μg/kg 범위로 나타났다. Immunoaffinity column 정제를 거쳐 HPLC-FLD로 분석한 결과 아플라톡신이 검출된 시료에 대해서 LC-MS/MS로 확인하였으며, 그 결과 모두 아플라톡신으로 확인되었다. 본 연구결과에서 나타난 곡류 및 견과류에 대한 아플라톡신 검출빈도 및 오염수준은 국내, 외 연구결과와 유사하거나 비교적 낮게 나타났으며 국내 아플라톡신 기준 및 미국, CODEX에서 설정된 기준규격 이하로 검출되었다. A survey for total aflatoxins (aflatoxins B<sub>1</sub>, B<sub>2</sub>, G<sub>1</sub>, and G<sub>2</sub>) was conducted on 245 cereals and processed cereal products, and 148 nuts and processed nut products in Korea, for a total of 393 commercialized samples. The total aflatoxins were quantified by the immunoaffinity column clean-up method with high performance liquid chromatography (HPLC) - fluorescence detection (FLD), and were confirmed by liquid chromatography tandem mass spectrometry (LCMS/ MS). Total aflatoxins (AFs) were detected in 37 samples (9.4% incidence), including 2 millet samples, 1 mixed cereal (sunsik), 1 powdered malt sample, 2 processed cereal products, 6 peanut samples, 22 peanut butter samples, and 1 sample each of almonds, adlay tea, and a processed nut product. The contamination levels were 0.04-2.65 μg/kg for aflatoxin B1, and 0.04 -5.51 μg/kg for total aflatoxins. Finally, LC-MS/MS analysis of the contaminated samples was conducted to confirm the detected aflatoxins, and all 37 samples showing aflatoxins by HPLC-FLD were confirmed by LC-MS/MS.
MK-801 투여에 의한 몰핀의존성랫드 뇌선조체중 도파민신경전달물질의 변화
이선희(Sun Hee Lee),신대섭(Dae Sup Shin),이덕주(Duck Joo Rhie),유영아(Young A Yoo),류승렬(Seung Rel Ryu),김대병(Dai Byung Kim),이종권(Jong Kwon Lee),김부영(Pu Young Kim) 한국응용약물학회 1998 Biomolecules & Therapeutics(구 응용약물학회지) Vol.6 No.1
The roles of dopamine(DA) and N-methyl-D-aspartate(NMDA) system in the development and expression of morphine dependence were investigated by monitoring the concentrations of extracellular DA and its metabolites by in vivo microdialysis and simultaneous observation of behavioral changes in morphinedependent rats. Extracellular DA level in caudate putamen of morphine-dependent rat was decreased and the concentrations of its metabolites, dihydroxy phenylacetic acid(DOPAC) and homovanillic acid(HVA), were increased during naloxone-precipitated withdrawal. DA contents were recovered to normal levels by pretreatment of MK-801, a noncompetitive NMDA receptor antagonist, which may explain the mechanism of diminishing effect of MK-801 on withdrawal symptoms in morphine-dependent rats. MK-801(0.3 mg/kg, i.p.) induced the untoward harmful neurological signs such as ataxia and severe rotations, which may be produced by hyperactivation of dopaminergic system. These results suggest that MK-801 may inhibit the expression of morphine dependence by altering the dopamine release.