Antimicrobial Effects of Omija Extracts on Food Poisoning Bacteria

Young Ah Yoo(유영아),Su Jin Kim(김수진),Dong Hyiun Yook(육동현),In Sil Yu(유인실),Kwon Jung(정권) 한국약용작물학회 2018 한국약용작물학회 학술대회논문집 Vol.2018 No.1

한국인 두경부암 환자에서 제3번 염색체 단완의 결손

손미나(Mi Na Son),유영아(Young A Yoo),조증근(Zeung Kenn Cho),최건(Kun Choi),최종욱(Jong Wook Choi),김열홍(Yeul Hong Kim),김준석(Jun Suk Kim) 대한두경부종양학회 1998 대한두경부 종양학회지 Vol.14 No.1

Objectives: Deletion in the short arm of chromosome 3 is common in many human cancers, including sporadic and hereditary renal carcinomas, small cell lung carcinomas, non-small cell lung carcinomas, and carcinomas of the ovary, breast, and cervix. A high frequency of chromosomal aberrations in head and neck cancers involving chromosome 3p has also been reported. These findings suggest that multiple tumor suppressor genes may be present on the short arm of chromosome 3. Materials and Methods: To investigate the possibility of chromosome 3p deletions in the Korean head and neck cancer patients, we applied a polymerase chain reaction(PCR)-based Restriction Fragment Length Polymorphism analysis to the DNA samples of matched normal mucosa and head and neck squamous cell carcinomas from 19 patients. Results: In the 19 normal samples heterozygosity at the polymorphic loci varied: 6 at the D3F15S2 locus(on telomeric 3p21), 2 at the D3S32 locus(on centromeric 3p21), and 4 at the THRB locus(on centromeric 3p24). In 12 matched carcinoma specimens, LOH(loss of heterozygosity) was observed at D3F15S2 in 1 of 6(17%), D3S32 in 1 of 2(50%), and at THRB in 2 of 4 cases(50%). Conclusion: The frequency of chromosome 3p deletion in the Korean head and neck carcinomas appear as other country did.

MK-801 투여에 의한 몰핀의존성랫드 뇌선조체중 도파민신경전달물질의 변화

이선희(Sun Hee Lee),신대섭(Dae Sup Shin),이덕주(Duck Joo Rhie),유영아(Young A Yoo),류승렬(Seung Rel Ryu),김대병(Dai Byung Kim),이종권(Jong Kwon Lee),김부영(Pu Young Kim) 한국응용약물학회 1998 Biomolecules & Therapeutics(구 응용약물학회지) Vol.6 No.1

The roles of dopamine(DA) and N-methyl-D-aspartate(NMDA) system in the development and expression of morphine dependence were investigated by monitoring the concentrations of extracellular DA and its metabolites by in vivo microdialysis and simultaneous observation of behavioral changes in morphinedependent rats. Extracellular DA level in caudate putamen of morphine-dependent rat was decreased and the concentrations of its metabolites, dihydroxy phenylacetic acid(DOPAC) and homovanillic acid(HVA), were increased during naloxone-precipitated withdrawal. DA contents were recovered to normal levels by pretreatment of MK-801, a noncompetitive NMDA receptor antagonist, which may explain the mechanism of diminishing effect of MK-801 on withdrawal symptoms in morphine-dependent rats. MK-801(0.3 mg/kg, i.p.) induced the untoward harmful neurological signs such as ataxia and severe rotations, which may be produced by hyperactivation of dopaminergic system. These results suggest that MK-801 may inhibit the expression of morphine dependence by altering the dopamine release.

LAS-Na 및 ASME(MES)의 피부 및 안자극 시험에 관한 연구

이호(Ho Lee),박원재(Won Jae Park),신대섭(Dae Sup Sin),이덕주(Duk Ju Lee),유영아(Young A Yoo),이도은(Do Eun Lee),이선희(Sun Hee Lee),김부영(Boo Young Kim) 한국실험동물학회 1993 Laboratory Animal Research Vol.9 No.2

교합방사선사진 촬영시의 흡수선량 계측

이상한,최갑식,유영아 大韓口腔顎顔面 放射線學會 1990 Imaging Science in Dentistry Vol.20 No.1

임상적으로 많이 사용하고 있는 교합촬영시의 두부 각 부위의 방사선 흡수량을 평가하기 위하여 황산칼슘(CaSO₄) 열발광선량계를 phantom(RT-210 Head & Neck Section(R), Humanoid Systems Co., U.S.A) 내부의 중요한 해부학적 구조물 10부위를 포함한 총 12부위에 위치시켜 70kVp, 15mA 상태 하에서 8인치 조사통에서는 1/4초, 16인치 조사통에서는 1초 동안 X-선을 노출시켜 상악 전치부 및 구치부의 부분악교합촬영, 하악전치부 및 구치부의 단면교합촬영과 부분악교합촬영시의 방사선 흡수량을 조사하여 아래와 같이 결과를 얻었다. 1. 전체적으로 가장 높은 방사선 흡수량을 보인 부분은 피부표면으로서 3264mrad에서 4073mrad 범위에 있었으며 각 촬영법에서의 차이는 크지 않았다. 2. 상악전치부 부분악교합촬영시 안구, 상악동, 뇌하수체부위가 다른 부위에 비해 방사선 흡수량이 많았으며 편측 상악구치부 부분악교합 촬영에서는 조사측안구와 조사측상악동부위에 많은 방사선 흡수량을 보였다. 3. 하악전치부 단면교합촬영시 안구부위를 제외한 모든 부위에서 비교적 낮은 방사선 흡수량을 보였다. 4. 편측 하악구치부 단면교합촬영시 조사측 안구부위와 조사측상악동 부위에서 다른 부위에 비하여 상대적으로 높은 방사선 흡수량을 보였다. 5. 하악전치부 부분악교합촬영시 양측상악도, 양측하악선, 갑상선부위에서 다른 부위에 비하여 높은 방사선 흡수량을 보였다. 6. 편측 하악구치부 부분악교합촬영시 조사측악하선부위에서 다른 부위에 비하여 높은 방사선 흡수량을 나타내었고 반대측안구에서도 상대적으로 높은 방사선 흡수량을 보였다. The purpose of this study was to estimate absorbed dose of each important anatomic site of phantom(RT-210 Head & Neck SectionR, Humanoid Systems Co., U.S.A.) head in occlusal radiography. X-radiation dosimetry at 12 anatomic sites in maxillary anterior topography, maxillary posterior topography, mandibular anterior cross-section, mandibular posterior cross-section, mandibular anterior topographic, mandibular posterior topographic occlusal projection was performed with calcium sulfate thermoluminescent dosimeters under 70 Kvp and 15 mA, 1/4 second(8 inch cone) and 1 second(16 inch cone) exposure time. The results obtained were as follows : Skin surface produced highest absorbed dose ranged between 3264 mrad and 4073 mrad but there was little difference between projections. In maxillary anterior topographic occlusal radiography, eyeballs, maxillary sinuses, and pituitary glad sites produced higher absorbed doses than those of other sites. In maxillary posterior topographic occlusal radiography, exposed eyeball site and exposed maxillary sinus site produced high absorbed doses. In mandibular anterior cross-sectional occlusal radiography, all sites were produced relatively low absorbed dose except eyeball sites. In mandibular posterior cross-sectional occlusal radiography, exposed eyeball site and exposed maxillary sinus site were produced relatively higher absorbed doses than other sites. In mandibular anterior topographic occlusal radiography, maxillary sinuses, submandibular glands, and thyroid gland sites produced high absorbed doses than other sites. In mandibular posterior topographic occlusal radiography, submandibular gland site of the exposed side produced high absorbed dose than other sites and eyeball site of the opposite side produced relatively high absorbed dose.

방사선조사가 백서 악하선 미세혈관과 내피세포에 미치는 영향

유영아 慶北大學校 齒科大學 1994 慶北齒大論文集 Vol.11 No.1

방사선조사에 따른 타액선내 미세혈관과 내피세포의 변화를 관찰하기 위하여 백서의 악하선에 6MV X선을 5Gy 및 10Gy 단일조사한 후 6시간, 12시간, 1일, 3일, 7일 그리고 14일에 희생시키고 투과전자현미경 및 H & E, PAS, Feulgen 염색을 시행하여 조직학적 변화를 관찰하고 다음의 결과를 얻었다. .미세혈관은 5Gy 조사후 12시간군에서 분포밀도의 증가가 나타났고, 3일군에서 밀도가 감소 되었으나 14일군에서는 다소 회복되었다. 10Gy 조사군의 소견도 유사하였으나 5Gy 조사군에 비해 반응정도가 빠르고 분명하였으며, 14일군에서도 미세혈관의 분포밀도는 감소되어 나타났다. . 선세포의 PAS 반응정도는 5Gy 조사후 6시간군에서 양성반응이 감소되었으나 1일군에는 약간 증가되었고, 3일군에서 다시 감소되는 경향을 보였으며, 14일군에서는 거의 정상으로 회복되었다. 이러한 소견은 10Gy 조사군에서도 유사하게 나타났으나 반응정도가 5Gy 조사군에 비해 분명하였다. . 혈관 내피세포 핵의 Feulgen 반응정도는 5Gy 조사후 6시간군에서 양성반응이 감소되었으며 1일군에서 다소 회복되었으나 3일군이후 다시 감소되었고, 14일군에서는 다소 회복되는 것으로 나타났다. 10Gy 조사군에서도 유사한 소견이 나타났으나 14일군에서도 회복되지 않았다. . 혈관 내피세포의 투과전자현미경 소견은 방사선조사직후 혈관내강의 축소와 세포질돌기의 미약한 증식이 관찰되었고, 1일군이후에서는 핵변성과 함께 세포질돌기와 세포질내 소포의 뚜렷한 증가, 그리고 기저막의 비후가 관찰되었다. 그후 5Gy 조사후 14일군에서는 회복되는 경향이었으나 10Gy 조사군에서는 정상으로 회복되지 않았으며, 전 실험군에서 기저막 소실이나 세포내 미세구조의 파괴는 관찰되지 않았다. . 이상의 소견으로 미루어 보아 방사선 조사후 악하선의 미세혈관은 내강의 축소와 내피세포 세포질돌기의 중식, 기저막의 비후 등이 나타나고, 이로 인한 영양 및 산소공급의 감소에 대한 대응조치로서 미세혈관의 분포밀도가 증가되고 소포 수의 증가를 통해 내피세포의 투과성이 항진되는 것으로 나타났으며, 이러한 변화는 5Gy 조사군에 비해 10Gy 조사군에서 크고 빠르게 나타났다. The purpose of this study was to investigate the irradiation effects on the capillary and endothelial cell in the submandibular gland. Sprague-Dawley strain male rats were singly irradiated to their neck region with the dose of 5Gy and 10Gy by 6MV X-irradiation and sacrificed on the 6 hours, 12 hours, 1, 3, 7, and 14 days after irradiation. The author observed the histological changes of the capillary at H & E staining, PAS staining and Feulgen staining under a light microscope, and also observed the ultrastructural changes of the endothelial cell using a transmission electron microscope. The results were as follows: In the light microscopic examination, the capillary density was slightly icreased on the 1 day after irradiation, and increased until the 7 days after irradiation. After then, capillary density was apparently decreased. The reaction to PAS staining at acinar cells was decreased on the 6 hours after irradiation, and recovered on the 7 days after irradiation. But reaction was decreased on the 14 days after irradiation again, after then, gradually recovered with days. The reaction to Feulgen staining at the capillary nucleus was decreased on the 6 hours after irradiation and recovered on the 1 day after irradiation. But the reaction was decreased on teh 3 and 7 days after irradiation again, after then, gradually recovered with days. In the transmission electron microscopic examination, mild proliferation of cytoplasmic process of the endothelial cell and reduction in luminal size were observed just after irradiation. AFter then, nuclear degeneration, marked proliferation of cytoplasmic process, thickened basal lamina, and numerous cytoplasmic vesicles were observed on the 1 day after irradiation. These changes were recovered to normal on the 14days after 5Gy irradiation group, but not with 10Gy irradiation group. And destruction of endothelial cell and loss of basal lamina were not observed in both groups. Form the above results, reduction in luminal size, proliferation of cytoplasmic process and thickening of basal lamina were observed as the irradiation effects on the capillary and endothelial cell of the submandibular gland. And also, these changes may induce increase in capillary number and endothelial permeability by means of increase of cytoplasmic vesicle formation. The changes appeared earlier and more prominent in 10Gy irradiated group than in 5Gy irradiated group.

방사선조사가 백서 악하선 미세혈관과 내피세포에 미치는 영향

유영아 경북대학교치과대학 1994 慶北齒大論文集 Vol.11 No.1

방사선조사가 악하선 미세혈관과 내피세포에 미치는 영향에 관한 실험적 연구

최갑식,최미,손정익,유영아,배용철 大韓口腔顎顔面 放射線學會 1994 Imaging Science in Dentistry Vol.24 No.1

The purpose of this study was to investigate the irradiation effects on the capillary and endothelial cell in the submandibular gland. Sprague-Dawley strain male rats were singly irradiated to their neck region with the dose of 5Gy and 10Gy by 6MV X-irradiation and sacrificed on the 6 hours, 12 hours, 1,3,7, and 14 days after irradiation. The authors observed the histological changes of the capillary at H & E and PAS staining under a light microscope, and also observed the ultrastructural changes of the endothelial cell using a transmission electron microscope. The obtaining results were as follows: 1. In the light microscopic examination, the capillary density was slightly increased on the 1 day after irradiation, and increased until the 7 days after irradiation. After then, capillary density was apparently decreased. 2. The reaction to PAS staining at acinar cells was decreased on the 6 hours after irradiation, and recovered on the 7 days after irradiation. But reaction was decreased on the 14 days after irradiation again, after then, gradually recovered with days. 3. In the transmission electron microscopic examination, mild proliferation of cytoplasmic process of the endothelial cell and reduction in luminal size were observed just after irradiation. After then, nuclear degeneration, marked proliferation of cytoplasmic process, thickened basal lamina, and numerous cytoplasmic vesicles were observed on the 1 day after irradiation. These changes were recovered to normal on the 14 days after 5GY group, but not with 10GY irradiation group. And destruction of endothelial cell and loss of basal lamina were not observed in both groups. 4. From the above results, reduction in luminal size, proliferation of cytoplasmic process and thickening of basal lamina were observed as the irradiation effects on the capillary and endothelial cell of the submandibular gland. And also, these changes may induce increase in capillary number and endothelial permeability by means of increase of cytoplasmic vesicle formation. The changes appeared earlier and more prominent in 10Gy irradiated group than in 5Gy irradiated group.