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월성 원자력발전소 주변 해양생물중의 조직결합 삼중수소 농도
김도성,박규준,임수경,김완,강희동,도시홍,김창규,Kim, Do-Sung,Park, Gyu-Jun,Lin, Xiu-Jing,Kim, Wan,Kang, Hee-Dong,Doh, Sih-Hong,Kim, Chang-Kyu 대한방사선방어학회 2003 방사선방어학회지 Vol.28 No.1
월성 원자력발전소 주변의 해양생물(해조류와 패류) 시료를 채취하여 조직결합 삼중수소 (OBT) 방사능농도를 측정 분석하였다. 해조류의 OBT 방사능농도는 $0.372{\sim}2.72$ Bq/kg-fresh의 범위였으며, 패류의 OBT 방사능농도는 $0.239{\sim}2.02$ Bq/kg-fresh의 범위였다. 그리고 해조류의 OBT에 의한 내부피폭선량은 $3.4{\times}10^{-7}mSv/year$로 평가되었다. The organically bound tritium(OBT) concentrations of the marine organisms(seaweed and shellfish) around Wolsung nuclear power plants were measured. The OBT concentrations of seaweeds were in the range of $0.372{\sim}2.72$ Bq/kg-fresh. The OBT concentrations of shellfish were in the range of $0.239{\sim}2.02$ Bq/kg-fresh. The internal dose due to the OBT concentration of seaweed was estimated as $3.4{\times}10^{-7}$ mSv/year.
박규미,김규준,Minghui Jin,Yongquan Han,소경하,현상환 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.12
Objective: We investigated how pituitary adenylate cyclase-activating polypeptide (PACAP) affects embryonic development during pre-in vitro maturation (pre-IVM) using porcine oocytes isolated from small follicles. Methods: We divided the follicles into the experimental groups by size (SF, small follicles; MF, medium follicles) and treated with and without PACAP and cultured for 18 hours (Pre-SF[-]PACAP; without PACAP, Pre-SF[+]PACAP; with PACAP) before undergoing IVM. The gene expression related to extracellular matrix formation (amphiregulin, epiregulin, and hyaluronan synthase 2 [HAS2]) and apoptosis (Bcl-2-associated X [BAX], B-cell lymphoma 2, and cysteine-aspartic acid protease 3) was investigated after maturation. The impact on developmental competence was assessed by the cleavage and blastocyst rate and total cell number of blastocysts in embryos generated from parthenogenesis (PA) and in vitro fertilization (IVF). Results: Cleavage rates in the Pre-SF(+)PACAP after PA were significantly higher than SF and Pre-SF(-)PACAP (p<0.05). The cleavage rates between MF and Pre- SF(+)PACAP groups yielded no notable differences after IVF. Pre-SF(+)PACAP displayed the higher rate of blastocyst formation and greater total cell number than SF and Pre-SF(-)PACAP (p<0.05). Cumulus cells showed significant upregulation of HAS2 mRNA in the Pre-SF(+)PACAP compared to the SF (p<0.05). In comparison to other groups, the Pre-SF(+)PACAP group displayed a downregulation in mRNA expression of BAX in matured oocytes (p<0.05). Conclusion: The PACAP treatment during pre-IVM improved the developmental potential of porcine oocytes derived from SF by regulating cumulus expansion and apoptosis of oocytes.