이중호박사 정년퇴임 기념논문집 : 스테비아로부터 천연 제초 활성물질의 분리 및 동정

권지웅 ( Ji Wung Kwon ),이중호 ( Joong Ho Lee ),이동헌 ( Dong Heon Lee ),권태오 ( Tae Oh Kwon ),김용현 ( Yong Hyun Kim ),노정래 ( Jung Rae Rho ),채규윤 ( Kyu Yoon Chai ) 원광대학교 생명자원과학연구소 2004 생명자원과학연구 Vol.27 No.1

연구논문 : 추출방법에 따른 복분자 추출물의 생리활성

권지웅 ( Ji Wung Kwon ),이희권 ( Hee Kwon Lee ),박희전 ( Hee Jeon Park ),송지영 ( Ji Young Song ) 한국식품조리과학회(구.한국조리과학회) 2012 한국식품조리과학회지 Vol.28 No.1

This study was carried out to evaluate biological activities concerning extracts according to extraction methods from unripened fruit of Rubus coreanus Miq. The extraction methods were HWE (hot water extraction for 4 hr at 100℃), SFE (extraction for 3 hr at 40℃ under 300 bar, 100% of CO2 fluid), USE (ultrasonification extraction for 4 hr at 50℃ with water), USE+HWE (hot water extraction for 2 hr at 100℃ after ultrasonification process for 2 hr), VE (vacuum extraction for 4 hr at 90℃ under 0.9 bar with water). VE extract showed the highest contents of total polyphenol (178.78±3.79 mg/g) and total flavonoid (40.93±0.68 mg/g). IC50 values of DPPH radical scavenging activity, linoleic acid peroxidation inhibition activity and LDL (low density lipoprotein) oxidation inhibition activity of HWE extract showed the lowest 35.39±0.25 μg/mL, 12.61±0.31 μg/mL and 1.31±0.02 μg/mL among other all extracts, respectively. IC50 values of α-glucosidase inhibitory activities of VE and HWE extracts showed lower 14.34±0.20 μg/mL and 15.83±0.20 μg/mL than those of other extracts, respectively. Specifically, HWE and VE extracts have relatively better biological activities than other extracts; these could be potentially used as a bioactive source for health functional foods.

자생식물 추출물로부터 발아억제물질의 탐색

권지웅 ( Ji Wung Kwon ),김보미 ( Bo Mi Kim ),이동헌 ( Dong Heon Lee ),권태오 ( Tae Oh Kwon ) 원광대학교 생명자원과학연구소 2008 생명자원과학연구 Vol.30 No.1

Opuntia ficus-indica 다당 A-1의 특성 및 알코올유도 간 산화스트레스의 보호 효과

류일환,권지웅,이어진,윤용갑,권태오,Ryu, Il-Hwan,Kwon, Ji-Wung,Lee, Eoh-Jin,Yun, Young-Gab,Kwon, Tae-Oh 대한한의학방제학회 2009 大韓韓醫學方劑學會誌 Vol.17 No.2

Reactive oxygen species(ROS) can induce hepatotoxicity and trigger apoptosis in the liver. In this study, we investigated the sulfated polysaccharide A-1 from Opuntia ficus-indica against alcoholic oxidative stress in human liver Hep G2 cell. An antioxidant substance A-1 obtained from the enzymatic extract of Opuntia ficus-indica fruit was purified by DEAE-cellulose ion exchange and sephadex G-100 gel permeation chromatography. The purification yield and molecular weight were 14.3% and 1.8 KDa, respectively. The A-1 predominately contained arabinose, galactose, rhamnose and also sulfate group. The structure of A-1 was investigated by periodate oxidation, FT-IR spectroscopy, $^1H$-NMR spectroscopy. The A-1 mainly composed of alternating unit of ${\rightarrow}4$)-$\alpha$-L- Rapp-2-$SO_3^-$-$\alpha$-L-Galp-($1{\rightarrow}$ and branched linkage of $\beta$-D-Arbp- ($5{\rightarrow}$. The antioxidative activity was measured using the SOD, CAT activity and GSH assay, respectively. The expression of Nrf2 protein was analyzed by western blotting. The viable cell count analyzed by autofluorescence. Oxidative stress induced by ethanol(1 M) were dramatically reduced by A-1 treatment. A-1 also prevented cell death induced by oxidative stress. It also increased expression Nrf2 protein level. We concluded that sulfated polysaccharide A-1 from Opuntia ficus-indica effectively protect Hep G2 liver cell from alcoholic oxidative stress.

초음파 처리에 의한 검정라즈베리 부위별 에탄올 추출물의 산화방지 활성

김기안(Ki An Kim),권지웅(Ji Wung Kwon),김용석(Yong-Suk Kim),박필재(Pill Jae Park),채규서(Kyu Seo Chae) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.4

본 연구는 검정라즈베리 부산물인 줄기, 잎 및 씨 초음파 추출물의 산화방지 활성에 미치는 영향을 살펴보고, 산화방지 활성을 가지는 식품 및 의약품의 개발 가능성을 알아보기 위하여 수행되었다. 검정라즈베리 부위별을 물 및 25, 50, 75, 100% 에탄올을 추출용매로 초음파 추출하여 실험을 진행한 결과 총 폴리페놀 함량의 경우 검정라즈베리 줄기의 25, 50% 에탄올 추출물이 각각 348.21±5.40, 343.39±5.94 ㎎/g으로 가장 높은 함량을 보였고, 잎은 50% 에탄올 추출물이 161.06±3.57 ㎎/g, 씨의 경우는 75% 에탄올 추출물이 266.29±2.51 ㎎/g으로 나타나 줄기의 총 폴리페놀 함량이 가장 높음을 확인하였다. 페놀성 화합물 10종의 분석 결과 모든 시료에서 갈산과 엘라그산이 가장 높은 함량의 화합물로 분석되었고, 쿼르세틴, 페룰산, 쿠마르산, 카페산, 루틴, 루테올린은 모든 추출물에서 분석되었다. 총 플라보노이드 함량을 측정한 결과에서는 추출용매간에 함량에 차이가 나타나는 것을 확인하였는데, 줄기는 25% 에탄올 추출물이 76.02±4.23 ㎎/mL로 가장 높은 함량을 보였고, 잎의 경우는 50% 에탄올 추출물이 82.28±0.54 ㎎/mL로 가장 높았으며, 씨의 경우는 75% 에탄올 추출물이 82.40±2.49 ㎎/mL로 가장 높게 나타났다. 산화방지 활성 검증을 위한 DPPH 및 ABTS 라디칼 소거활성의 EC<SUB>50</SUB> 값을 비교한 결과 총 폴리페놀 함량이 가장 높았던 줄기의 활성이 가장 높았으며, 씨>잎 순으로 높은 라디칼 소거활성을 보였고, 추출용매 조건에 따라서 활성의 차이가 크게 나타났다. 또한 환원력을 측정한 결과에서도 줄기>씨>잎 순으로 높은 환원력을 보였고, 검정라즈베리 부산물인 줄기, 잎 및 씨 모두 높은 수준의 산화방지 활성을 가지는 것으로 확인되었으며, 추출조건으로는 줄기의 경우 25, 50% 에탄올, 잎의 경우 50% 에탄올, 씨의 경우 75% 에탄올 용매 조건에서 산화방지 활성이 가장 우수한 것으로 나타났다. 결과적으로 검정라즈베리 부산물인 줄기, 잎 및 씨 모두 산화방지 활성이 우수한 천연소재로서의 이용이 가능할 것으로 판단되었고, 특히 줄기의 경우 산화방지 활성이 매우 뛰어나 건강기능 식품 및 의약품과 같은 산업적 이용 가치가 높을 것으로 판단된다. This study was carried out to investigate the antioxidant effects of different parts (stems, leaves, and seeds) of the black raspberry for utilization as food materials. Different parts of the black raspberry were subjected to extraction via ultra-sonication extraction methods using water and ethanol at various concentrations (25, 50, 75, and 100%). Antioxidant capability of the extracts were determined by amounts of phenolic compounds, with flavonoid contents, radical scavenging activity, and reducing power. Irrespectively of ethanol concentration, extracts of stem showed the highest total phenolic compounds and antioxidant activities among different parts of black raspberry. The total phenolic compounds extracted from the black raspberry stem using 25 and 50% ethanol showed 348.21±5.40 and 343.39±5.94 ㎎/g, respectively. Fifty percent ethanol extracts of the black raspberry stem showed the highest DPPH (EC50 value: 60.89 ㎍/mL) and ABTS radical scavenging activities (EC50 value: 82.57 ㎍/mL). Further, 25% ethanol extacts of the black raspberry stem (0.263±0.004) was found to have the highest reducing power. The highest antioxidant activity of black raspberry stem indicates that black raspberry stem may be useful source for functional food.

토종효모 Saccharomyces cerevisiae GBY5를 이용한 오디발효주의 발효 특성 및 항산화 활성

류은혜(Eun-Hye Ryu),채규서(Kyu-Seo Chae),권지웅(Ji-Wung Kwon) 동아시아식생활학회 2019 동아시아식생활학회지 Vol.29 No.3

Mulberry wine was manufactured using four strains isolated from mulberry and aronia to select the strain most suitable for mulberry wine production. Among the yeast strains tested, Saccharomyces cerevisiae GBY5 (S. cerevisiae GBY5), named for its high ethanol production and strong red hue, was selected as the final fermentation strain to manufacture the wine. Traditional yeast (S. cerevisiae GBY5) and Saccharomyces cerevisiae Fermivin (S. cerevisiae Fermivin), a product that is used widely for commercial wine production, was added to the mulberries at a concentration of approximately 1×10<SUP>9</SUP> CFU/kg, followed by incubation at 25℃ for 6 days. The final products of the mulberries fermented with S. cerevisiae GBY5 contained 13.33% alcohol, 8.93 °Brix, and 0.78% total acidity. In the mulberries fermented with S. cerevisiae Fermivin, the final product contained 13.24% alcohol, 9.23 °Brix, and 0.81% total acidity. The lightness of the wine made with S. cerevisiae Fermivin was higher than that made with S. cerevisiae GBY5, but the redness and yellowness of S. cerevisiae GBY5 were higher than those made with S. cerevisiae Fermivin. The concentrations of malic and citric acid were higher in the wine fermented with S. cerevisiae GBY5, whereas the concentration of acetic acid was higher in the wine fermented with S. cerevisiae Fermivin. Finally, the total polyphenol content of S. cerevisiae GBY5 was higher than made with S. cerevisiae Fermivin. The DPPH radical scavenging activity of S. cerevisiae Fermivin was as high as that of S. cerevisiae GBY5, but the ABTS radical scavenging activity of S. cerevisiae GBY5 was higher than that of S. cerevisiae Fermivin. In conclusion, the traditional yeast S. cerevisiae GBY5 produced mulberry wine with a higher alcohol concentration than S. cerevisiae Fermivin and exhibited excellent redness and antioxidant activity. Thus, S. cerevisiae GBY5 can replace S. cerevisiae Fermivin in industrial wine production.

복분자 미숙과 물추출물의 콜레스테롤 개선 효과

최혜란(Hye Ran Choi),이수정(Su Jung Lee),이정현(Jung-Hyun Lee),권지웅(Ji Wung Kwon),이희권(Hee Kwon Lee),정종태(Jong Tae Jeong),이태범(Tae-Bum Lee) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.12

We investigated the effects of unripe black raspberry water extract (UBR-W) and oxidation-LDL treatment on cholesterol levels. Experiments using an established human hepatocellular carcinoma cell line (HepG2) showed a time-dependent increase in expression of LDL receptor after UBR-W treatment. Expression of LDL receptor-related genes, such as SREBP1 and 2, increased upon UBR-W treatment. However, expression of HDL-related genes was unaffected by UBR-W. HMG-CoA reductase activity was reduced by UBR-W treatment, whereas HMG-CoA mRNA expression significantly increased. In addition, the ApoB/ApoA1 mRNA level, which is a predictor of cardiovascular risk, was reduced in a time-dependent manner by UBR-W treatment. Macrophage-like cells (RAW 264.7) showed increased expression of ox-LDL-related genes, such as CD36, scavenger receptor-A, adipophilin, and PPAR-gamma, upon ox-LDL treatment compared to untreated control cells, and quantitative lipid analysis indicated a dramatic increase in lipid accumulation. However, UBR-W treatment significantly reduced expression of ox-LDL-related genes and largely prevented lipid accumulation. The results indicate that UBR-W mediates a cholesterol-lowering effect via inhibition of cholesterol synthesis and induction of LDL uptake through SREBP.

돌나물의 삽목 및 기내번식에 미치는 삽수부위, 크기와 Cytokinin의 영향

이승엽(Seung Yeob Lee),안정호(Jeong Ho Ahn),권지웅(Ji Wung Kwon),권태오(Tae Oh Kwon),이중호(Joong Ho Lee) 한국원예학회 2003 한국원예학회 학술발표요지 Vol.2003 No.5

Pectinase를 이용한 복분자, 오디, 아로니아 과즙의 항산화 활성

손락호(Son Rak Ho),채규서(Chae Kyu Seo),김기안(Kim Ki An),권지웅(Kwon Ji Wung),박재숙(Park Jae Suk),조하니(Cho Ha Ni) 한국약용작물학회 2014 한국약용작물학회 학술대회논문집 Vol.2014 No.2

복분자 미숙과 추출물이 전립선암 세포주와 전립선비대 백서모델에 미치는 영향

이수정(Su Jung Lee),최혜란(Hye Ran Choi),이정현(Jung-Hyun Lee),권지웅(Ji Wung Kwon),이희권(Hee Kwon Lee),정종태(Jong Tae Jeong),이태범(Tae-Bum Lee) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.4

본 연구는 전립선암세포주인 LNCaP 세포주와 테스토스테론으로 유도된 전립선비대 백서모델에서 복분자 추출물의 전립선비대 억제 효과를 조사하였다. 첫째, 전립선암 세포주(LNCaP)에서 복분자 미숙과 추출물의 안드로겐 관련 전립선비대 유전자 억제 효과를 조사한 결과 미숙과 50% 에탄올 추출물은 안드로겐 수용체(AR)뿐만 아니라 전립선특이항원(PSA)과 5-알파 환원 효소 type 2(5AR2)의 발현을 가장 높게 억제하였다. 또한 LNCaP 세포에 DHT로 안드로겐 관련 유전자를 유도한 후 복분자 미숙과 50% 에탄올 추출물을 처리한 결과 AR과 PSA mRNA의 발현이 억제됨을 확인하였다. 둘째, 테스토스테론을 이용하여 전립선비대를 유도한 동물모델에서 복분자 미숙과 50% 에탄올 추출물을 6주간 투여한 후 전립선비대 개선 효과를 조사한 결과, 전립선비대 유발군에 복분자 미숙과 추출물을 투여한 군에서 전립선 무게, 전립선 소포의 상피세포 두께 및 면적이 감소함을 확인할 수 있었고, 전립선비대 유발 호르몬인 DHT level이 감소함을 확인할 수 있었다. Benign prostatic hyperplasia (BPH), which is commonly found in aging men, is characterized by hyperplasia of prostatic stromal and epithelial cells beginning in the periurethral zone of the prostate. The prevalence of BPH increases in an age-dependent manner. Here, we investigated the protective effects of unripe Rubus occidentalis extracts (UROE) on BPH development using a prostate cancer cell line and testosterone-induced BPH rat model. Experiments using an established hormone-dependent prostate cancer cell line (LNCaP) showed that UROE treatment significantly decreased expression of androgen-related genes, including androgen receptor (AR), prostate specific antigen (PSA), and 5-alpha reductase 2, but not 5-alpha reductase 1, which was also observed in flutamide-treated cells. Further, AR and PSA gene expression was reduced by UROE treatment under androgen-stimulated conditions using dihydrotestosterone (DHT). BPH animals displayed elevated prostate weights. However, UROE as well as finasteride treatment significantly reduced prostate weights and DHT levels compared to testosterone-induced BPH animals. Histopathological analysis also showed that UROE treatment suppressed testosterone-induced prostatic hyperplasia. Taken together, the results suggest that UROE may effectively inhibit the development of BPH and thus may be a useful agent in BPH treatment.