새 시대의 새 공무원 양성

권기상 대한지방행정공제회 1974 地方行政 Vol.23 No.250

임산부의 신체적조건이 제왕절개술 빈도에 미치는 영향

권기상,김창규,서경,황동훈,박찬규 대한산부인과학회 1986 Obstetrics & Gynecology Science Vol.29 No.7

체계 개발 단계별 기계 부품에 대한 신뢰도 예측 시 NSWC-98-LE1 적용 방안

권기상,박은심,조차현,이동우,이수중,Kwon, Ki Sang,Park, Eun Sim,Cho, Cha Hyun,Lee, Dong Woo,Lee, Su Jung 한국시스템엔지니어링학회 2008 시스템엔지니어링학술지 Vol.4 No.1

Generally, in analysis of reliability of Design and Development Phase, reliability of electrical components is analyzed based on standards such as MIL-HDBK-217F, Bellcore Issue 4,5,6 by analyzing stress of architectural side (Power, Voltage, Current and quality level of components) of weapon system and stress of operational side (operational environment, operational temperature, Operational Profile). But the reliability of mechanical components is analyzed based on the data book of failure history of mechanical parts called NPRD-95(Nonelectronic Parts Reliability Data-95) without any analysis of above stress. However, even if it's the same mechanical parts, it might have different failure rate(fatigue, wear, corrosion) during operation depending on how weary(stress : pressure, vibration, temperature during operation) the parts are. Therefore, analyzing reliability using just data book can cause big difference in reliability instead of analyzing based upon stressfulness that parts might have, operational concept, and other various factors. Thus, This paper will guide the way of predicting reliability by organizing ways of predicting reliability for system organization and adopt ing NSWC-98/LE1(Naval Surface Warfare Center-98/LE1) for mechanical components.

Copper Regulates Apelin Expression in L6 Skeletal Muscle Cells

권기상,박진솔,최영은,이은령,유재은,박혜원,권오유 한국생명과학회 2023 생명과학회지 Vol.33 No.9

In this experiment, we aimed to investigate the role of copper in regulating the biosynthesis of a myokine called apelin in mammalian skeletal muscle cells. Our approach involved culturing skeletal muscle cells and subjecting them to treatments with copper sulfate or a copper chelator known as bathocuproinedisulfonic acid (BCS). We employed standard techniques, such as reverse transcription- polymerase chain reaction (RT-PCR) and Western blotting, to assess the synthesis of apelin at different stages, including transcription, translation, and post-translational modifications. Our findings demonstrated that copper had an inhibitory effect on apelin biosynthesis at all three stages: transcription, translation, and post-translation. However, when we treated the cells with BCS, the biosynthesis of apelin was restored to its original state. This finding suggests that copper is required for the synthesis of apelin in mammalian skeletal muscle cells. This study represents the first documented evidence of the inorganic copper-dependent regulation of apelin biosynthesis, shedding light on potential strategies for the prevention and treatment of sarcopenia induced by copper imbalances.

Batch Blending & Scheduling Problem

권기상,Faramroze Engineer,이세찬 한국경영과학회 2016 한국경영과학회 학술대회논문집 Vol.2016 No.4

Characterization of a cDNA Encoding Transmembrane Protein 258 from a Two-spotted Cricket Gryllus bimaculatus

권기상,김홍근,박혜원,권오유 한국생명과학회 2023 생명과학회지 Vol.33 No.10

The cDNA that encodes transmembrane protein 258 (Tmem258) was cloned from Gryllus bimaculatus and named GbTmem258. This protein comprises 80 amino acids, has no N-glycosylation site, and contains five potential phosphorylation sites at two serines, two threonines, and one tyrosine. The predicted molecular mass of GbTmem258 is 9.06 kDa, and its theoretical isoelectric point is 5.5. The tertiary structure of GbTmem258 was predicted using the available secondary structure information, which suggests the presence of alpha helices (52.5%), random coils (22.5%), extended strands (16.25%), and beta turns (8.75%). Homology analysis revealed that GbTmem258 exhibits high similarity at the amino-acid level to Tmem258 found in other species. The effect of starvation and refeeding on GbTmem258 mRNA expression was also examined in this study. It was found that GbTmem258 mRNA expression in the hindgut progressively increased throughout the starvation period, peaking at almost 1.5 times the control level after six days of starvation. However, refeeding for one to two days after the six-day starvation period restored GbTmem258 mRNA expression to the control level. In fat body, GbTmem258 mRNA expression was almost 3-fold higher during starvation compared to the control level. Refeeding for one to two days after the six-day fast resulted in a decline in the expression to about a 2.5-fold increase over the control level. Throughout the starving and refeeding periods, no other tissues showed any discernible alterations in GbTmem258 mRNA expression.

Batch Blending & Scheduling Problem

권기상,Faramroze Engineer,이세찬 대한산업공학회 2016 대한산업공학회 춘계학술대회논문집 Vol.2016 No.4

SK이노베이션 Optimization & Analytics Lab 소개

권기상 대한산업공학회 2018 ie 매거진 Vol.25 No.1

장수풍뎅이(Korean Rhinoceros Beettle, Allomyrina dichotoma)에 Oryctes rhinoceros nudivirus 감염 특이적으로 발현하는 유전자 동정

권기상(Kisang Kwon),유보경(Bo-Kyung Yoo),서현우(Hyun-Woo Suh),고영화(Young Hwa Ko),김홍근(Hong Geun Kim),이석현(Seokhyun Lee),박관호(Kwan-Ho Park),최지영(Ji-Young Choi),권오유(O-Yu Kwon) 한국생명과학회 2015 생명과학회지 Vol.25 No.8

장수풍뎅이(Korean Rhinoceros Beettle, Allomyrina dichotoma)는 관상용으로 인기가 높으며, 애벌레는 식품으로 주목을 받고 있다. 장수풍뎅이의 대량사육 시 발생하는 질병의 원인이 최근에 virus (Oryctes rhinoceros nudivirus)인 것이 보고되었다. 그러나 아직 정확한 발병기전과 치료방법을 알지 못하여 사육농가에 심각한 경제적 손실을 주고 있다. 본 연구에서는 virus에 감염된 장수풍뎅이의 fat body와 장에서 심각한 조직변화가 관찰되었다. 이 때에 유전자발현이 상승하는 것으로 BTF3H4-like, SPS-like, COPB1, T-CP, HSP70를 보고한다. 이 결과는 장수풍뎅이의 대량사육 시 발생하는 질병의 조기진단과 치료에 실마리를 제공할 것이다. 그리고 안정적인 생산성 향상을 통해서 소득증대 효과 및 균일화된 대량사육을 통해서 식품원료로 인정받을 수 있을 것이다. The Korean rhinoceros beetle (Allomyrina dichotoma) is popular as a pet and as a food ingredient, and it is commercially distributed in Korea. It is also traditionally regarded as a medicine for liver-related diseases. Recently, the Oryctes rhinoceros nudivirus was introduced from Southeast Asia. This virus is reported as a disease factor for A. dichotoma in mass-rearing facilities, and economic losses due to this viral infection have been increasing in Korea since the 2010s. In this study, we observed serious structural changes in the fat body and the intestine of virus-infected beetles. We report five genes that are up-regulated by the viral infection in the intestine: BTF3H4-like (transcription factor BTF3 homolog 4-like), SPS-like (serine proteinase stubble-like), COPB1 (coatomer protein complex, subunit beta 1), T-CP (T-complex 1 subunit gamma), and HSP70 HSP70 (heat shock protein 70). The results may provide a clue for the early diagnosis and disease-treatment that occurs in mass-rearing facilities. The improvement of stable productivity will increase the farmers’ income, and quality control of beetle-breeding will help industries to utilize this beetle as a promising food ingredient.

소포체스트레스 센서 OASIS family의 분자기전

권기상(Kisang Kwon),김승환(Seung-Whan Kim),유권(Kweon Yu),권오유(O-Yu Kwon) 한국생명과학회 2015 생명과학회지 Vol.25 No.4

진핵세포의 소포체는 분비를 담당하는 첫 번째 기관이다. 대부분의 분비단백질과 막 형성단백질은 소포체에서 세포질/핵으로 전달되는 신호전달에 의한 번역후수식에 의해서 소포체를 통해서 분비된다. 그 결과 완전하게 접힘이 일어난 단백질만 세포 밖으로 분비된다. 소포체내에서 완전하게 접힘이 일어나지 않아 축적된 단백질은 세포내스트레스(소포체스트레스)가 되어 unfolded protein response (UPR)시스템을 작동시킨다. UPR을 작동시키는 3종류의 소포체막단백질은 inositol requiring 1 (IRE1), PKR-like ER kinase (PERK), activating transcription factor 6 (ATF6)이 존재한다. 최근에 새로운 종류의 ATF6이 동정되었다. 이들은(Luman, OASIS, BBF2H7, CREBH, CREB4) 공통적으로 소포체막관통영역, 전사활성영역, bZIP영역을 가지며 특이조직과 세포내기관에서 기능을 가진다. 현재로서는 OASIS family의 정확한 분자기전 설명은 어렵지만, 본 리뷰에서 이들 분자신호를 포괄적으로 소개할 것이다. The endoplasmic reticulum (ER) in the eukaryotic cells is the first compartment in the secretory pathway. Almost secretory proteins and membrane proteins are secreted through the ER, in which post-translational modifications occur via diverse signals from the ER lumen to the cytoplasm and nucleus. Only then are correctly-folded proteins secreted to the outside cells. Unfolded proteins that accumulate in the ER cause a kind of intracellular stress, ER stress, and activate an unfolded protein response (UPR) system. The 3 major transducers of the UPR are inositol requiring 1 (IRE1), PKR-like ER kinase (PERK) and activating transcription factor 6 (ATF6), all of which are ER transmembrane proteins. Recently, novel types of a new ATF6 family have been identified. Those commonly have an ER-transmembrane domain, a transcription-activation domain and a basic leucine zipper (bZIP) domain-Luman, OASIS, BBF2H7, CREBH and CREB4. Each factor functions by regulating the UPR in specific organs and tissues. Although the detailed molecular mechanisms of OASIS family members are unknown, in this study we comprehensively introduce these molecular signals.