장내세균의 락토오스 분해 유전자 확인 연구

고유라,김가은,현성희 국제차세대융합기술학회 2023 차세대융합기술학회논문지 Vol.7 No.12

유당 분해능을 확인하는 시험은 대표적으로 MacConkey 배지 배양과 ONPG 시험이다. 그러나 일부 균주 들은 유당을 분해함에도 두 시험의 생화학적 성상이 다르게 나타난다. 이에 그람 음성 장내세균 중 Escherichia coli를 포함한 12개 균주를 사용하여 유당 분해 능력을 확인하였다. 유당의 빠른 발효능력은 MacConkey 배지에서, 유당의 느린 분해능력은 ONPG disk를 사용하여 확인하였다. 12개 균주의 DNA를 추출하여 PCR 증폭과 증폭산물 의 염기서열분석법으로 Lac Z와 Lac Y 유전자를 확인하였다. Escherichia coli 외 4개의 균주에서는 MacConkey 배 지 양성과 ONPG 시험 양성으로 두 유전자가 모두 확인되었다. Edwardsiella tarda 외 4개의 균주는 MacConkey 배지 음성과 ONPG 시험 음성으로 두 유전자 모두 확인되지 않았다. Serratia marcescens는 MacConkey 배지 음성 과 ONPG 시험 양성으로 β-galactosidase 유전자는 확인되었으나 lactose permease 유전자는 확인되지 않았다. 본 연구에서 이러한 유당 분해 능력의 차이는 Lac Z (β-galactosidase)와 Lac Y (lactose permease) 두 가지 유전 자의 존재 유무에 따라 다르다는 것을 확인하였다. Tests for lactose degrading abilities are typically MacConkey medium culture and ONPG test. However, Some enterobacteriacaes show different biochemical properties of the two tests even though they degrade lactose. Lactose degradation ability was confirmed using 12 strains, including Escherichia coli. The rapid fermentation ability of lactose was observed through proliferation and color changes of neural red, which is a pH meter in MacConkey agar medium. The low fermentation ability of lactose was observed through ONPG disk. DNA of 12 strains, including Escherichia coli, was extracted and Lac Z and Lac Y genes were confirmed by PCR amplification and sequencing of amplified products. In five strains including Escherichia coli, both β-galactosidase and lactose permease genes were identified as MacConkey agar and ONPG test were both positive. In four other strains including Edwardsiella tarda, MacConkey agar and ONPG test both negative, and neither β-galactosidase nor lactose permease genes were identified. Serratia marcescens was negative for MacConkey agar and positive for ONPG test, and not lactose permease gene, but β-galactosidase gene was identified. Klebsiella pneumoniae is MacConkey agar positive and ONPG test positive, and both β-galactosidase and lactose permease genes have been identified, but it degrades lactose slowly. In this study, it was confirmed that the difference in lactose degradation ability depends on the presence or absence of two genes, Lac Z (β-galactosidase) and Lac Y (lactose permease).

전처리 유무에 따른 동애등에의 음식물 쓰레기 처리효율

고유라,이유석,전태영,김재용 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1

2011년 기준으로 전 세계 생산 음식물이 거의 3분의 1에서 최고 50%까지 쓰레기로 버려지고 있다. 2005년부터 음식물쓰레기를 직·매립하는 것이 금지되고, 2013년부터 런던협약으로 인하여 음식물쓰레기의 해양투기가 금지되어 음식물 쓰레기의 처리 문제가 많은 이슈가 되고 있다. 따라서 친환경적으로 음식물 쓰레기를 처리하는 방법이 대두되고 있는 가운데 동애등에를 이용한 음식물 쓰레기 처리법이 많은 주목을 받고 있다. 본 연구에서는 전처리에 따른 동애등에의 음식물 쓰레기 처리효율을 알아 보았다.

『枕草子』「宮にはじめてまゐりたるころ」の章段攷 : 「身のほど」を中心として

高有貞(고유정) 일본어문학회 2008 일본어문학 Vol.43 No.-

『枕草子』「里にまかでたるに」章段攷 - 則光をめぐって

高有貞(고유정) 일본어문학회 2006 일본어문학 Vol.34 No.-

Symptoms and pharmacokinetic stages of acute L-thyroxine poisoning in children

고유라,이지숙 대한응급의학회 2022 대한응급의학회 학술대회초록집 Vol.2022 No.2

한국여성에서 체질량지수(Body Mass Index)에 따른 유방치밀도의 차이

고유라,신기원,서윤영,고정아,박민선,조비룡,배우경 대한임상건강증진학회 2008 Korean Journal of Health Promotion Vol.8 No.4

연구배경 유방촬영술상 치밀유방은 유방암의 위험요소일 뿐 아니라 검사의 민감도를 감소시켜 위음성의 중요한 요소가 된다. 한국여성에서 비만도와 유방치밀도와의 관계는 알려진 바가 적다. 본 연구에서는 한국여성에서 체질량지수와 치밀유방과의 연관성을 파악하고 이를 바탕으로 적절한 유방암검진방법을 모색해보고자 하였다. 방 법 2000년 2월부터 2006년 3월까지 건강검진센터에서 유방촬영술을 시행한 무증상의 여성 16482명을 대상으로 연령과 체질량지수에 따른 유방실질의 밀도분포를 분석하였으며 추가적인 유방초음파 촬영을 권유 받은 경우를 분석하였다. 유방촬영술상 유방실질의 밀도는 ACR BI-RADS의 유방실질분류를 따랐다. 결 과 치밀유방의 빈도는 4275명으로 25.9%였으며 연령과 체질량지수가 증가할수록 각각 감소하는 경향을 보였다. 연령으로 보정한 후의 치밀유방의 위험도는 정상체중에 비하여 저체중의 경우 1.381배 높았고 과체중의 경우 0.792배, 비만의 경우 0.540배, 고도비만의 경우 0.298배로 감소하는 경향을 보였다. 결 론 한국여성에서 체질량지수는 연령으로 보정한 후에도 치밀유방과 음의 상관관계를 갖는다. Background It is well known that low BMI and younger age are associated with high breast density in western women, but there is a lack of researchin Korean women. Mammographic dense breast has been reported both as a cause of false-negative findings on mammography and as an indicator of increased breast cancer risk. The purpose of this study was to evaluate the relationship between breast density and BMI. Furthermore, we considered proper screening method of breast cancer in Korean women. Methods From February 2000 to March 2006, 16,482 asymptomatic women who underwent screening mammography in the health promotion center were included. Those with prior history of breast cancer or breast surgery were excluded. Breast density distribution and further breast sonography recommendation rate were evaluated. Results 25.9% of women (n=4,275) had dense breasts. Both Age and BMI were negatively associated with breast density respectively (P<0.001, P<0.001). BMI after adjusting for age was negatively associated with breast density (OR of underweight 1.381, 95% CI 1.139-1.674; OR of overweight 0.792, 95% CI 0.723-0.868; OR of obesity 0.540, 95% CI 0.488-0.597; OR of severe obesity 0.298, 95% CI 0.217-0.409). 17.8% (n=2,939) of women were recommended further breast sonography due to low sensitivity of mammography. Young age and low BMI were associated with sonography recommendation rate (P<0.001, P<0.001). Conclusions BMI is negatively associated with breast density after adjusting for age. Mammography combined with breast sonography may increase sensitivity of breast cancer screening in especially young and thin women in Korea.

Comparison of Base Deficit and Vital Signs as Criteria for Hemorrhagic Shock Classification in Children with Trauma

고유라,김중헌,황경진,이지숙,허요 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.4

Purpose: Base deficit (BD) is superior to vital signs in predicting trauma outcomes in adults. The authors aimed to compare BDand vital signs as criteria for the four-tiered hemorrhagic shock classification in children with trauma. Materials and Methods: We retrospectively reviewed the data of 1046 injured children who visited a Korean academic hospitalfrom 2010 through 2018. These children were classified separately based on BD (class I, BD ≤2.0 mmol/L; II, 2.1–6.0 mmol/L; III,6.1–10 mmol/L; and IV, ≥10.1 mmol/L) and vital signs (<13 years: age-adjusted hypotension and tachycardia, and Glasgow ComaScale; 13–17 years: the 2012 Advanced Trauma Life Support classification). The two methods were compared on a class-by-classbasis regarding the outcomes: mortality, early transfusion (overall and massive), and early surgical interventions for the torso ormajor vessels. Results: In total, 603 children were enrolled, of whom 6.6% died. With the worsening of BD and vital signs, the outcome rates increasedstepwise (most p<0.001; only between surgical interventions and vital signs, p=0.035). Mortality more commonly occurredin BD-based class IV than in vital signs-based class IV (58.8% vs. 32.7%, p=0.008). Early transfusion was more commonly performedin BD-based class III than in vital signs-based class III (overall, 73.8% vs. 53.7%, p=0.007; massive, 37.5% vs. 15.8%, p=0.001). No significantdifferences were found in the rates of early surgical interventions between the two methods. Conclusion: BD can be a better predictor of outcomes than vital signs in children with severe hemorrhagic shock.

학령전기 유소아 중독의 특징; 내겐 너무 매력적인 알약

고유라,이지숙,장민,이은해,양희원,곽재령 대한응급의학회 2018 대한응급의학회 학술대회초록집 Vol.2018 No.2

The impact of Omicron wave on pediatric febrile seizure

고유라,정재형,양희원,최유진,이지숙 대한응급의학회 2022 대한응급의학회 학술대회초록집 Vol.2022 No.2

우리나라 청소년에서 수면시작시간과 우울감의 상관관계 : 청소년 건강행태온라인조사를 바탕으로

고유라 한국학교보건학회 2016 韓國學校保健學會誌 Vol.29 No.2

Purpose: The purpose of this study was to investigate the relationship between sleep timing and depressive mood in Korean adolescents. Methods: The study analyzed the data from the 2007~2015 Korea Youth Risk Behavior Web-based Survey. A total of 541,693 students in grades 7~12 were included in the final analysis. Multivariable logistic regression was used to examine their sleep timing and depressive mood, adjusted for sex, grade, region, socioeconomic status, academic performance, alcohol, smoking and physical activity. Sleep duration and sleep quality were also included in our model to identify whether or not the effect of sleep timing on depression is mediated by sleep duration or sleep quality. Results: The prevalence of depressive mood was 32.7% and the mean sleep timing was 12:13 AM. After adjustment for eligible covariates, the association between sleep timing and depressive mood showed a J-shaped curve. Adolescents who slept at 8 pm~10 pm were 39% more likely to be depressive (OR = 1.39, 95% CI 1.30~1.40) and at 3 am~ 4 am were 67% more likely to be depressive (OR=1.67, 95% CI 1.64~1.70) than adolescents who slept at 11 pm~12 am. These associations persisted after being adjusted for sleep duration and sleep quality. Conclusion: Sleep timing was related to depression in adolescents, independent of sleep duration and sleep quality. It appears that there is a certain sleep timing beneficial to mental health of adolescents.