Comamonas sp. Strain DJ-12로부터 Protocatechuate의 분해에 관여하는 pmcABCDEFT 유전자군의 구조 분석

강철희,이상만,이경,이동훈,김치경,Kang Cheol-Hee,Lee Sang-Mhan,Lee Kyoung,Lee Dong-Hun,Kim Chi-Kyung 한국미생물학회 2005 미생물학회지 Vol.41 No.3

Comamonas sp. strain DJ-12의 pmcABCDEFT 유전자군은 protocatechuate (PCA)의 분해과정에 관여하는 PCA 4,5-dioxygenase, 4-carboxy-2hydroxymuconic semialdehyde (CHMS) dehydrogenase, 2-pyrone04,5-dicarboxylate(PDC) hydrolase, 4-oxalomesaconate (OMA) hydratase, 그리고 4-oxalocitramalate (OCM) aldolase 등의 효소들을 생산하는 유전자들과 transporter의 역학을 하는 유전자로 각각 확인되었다. 이 유전자군은 Comamonas sp. strain DJ-12의 chromosomal DNA로부터 얻은 PCR 산물들을 T-vector에 ligation하여 재조합 플라스미드 pMT1, pMT2, pMT3, pMT4, pMT5, pMT6, pMT7, pMT8, pMT9, pMT10을 제조하였다. 이들 재조합 플라스미드의 염기서열을 분석한 결과 PCA 4,5-dioxygenase 유전자는 alpha(pmcA)와 beta(pmcB) 두 개의 subunit으로 구성 되어있으며, 각각 450 bp와 870 bp이었다. CHMS dehydrogenase 유전자(pmcC)는 960 bp, PDC hydrolase 유전자(pmcD)는 918 bp이였으며, OMA hydratase 유전자(pmcE)는 1029 bp, OCM aldolase 유전자 (pmcF)는 689 bp, 그리고 transporter 유전자(pmcT)는 1,398 bp이였다. 이들 pmc 유전자들은 pmcT-pmcE-pmcF-pmcD-pmcA-pmcB-pmcC의 순서로 배열되어 있었다. Comamonas sp. strain DJ-12의 pmcABCDEFT 유전자산물의 아미노산 서열을 분석한 결과, Comamonas testosteroni BR6020 및 Psedomonas ochraceae NG.J1와 $94{\~}98\%$의 높은 유사성을 보였고, 그 유전자들의 배열 순서도 동일하였다. 그러나 Sphingomonas paucimobilis SYK-6, Sphingomonas sp. LB126, 그리고 Arthrobacter keyser 12B와는 아미노산 서열이 $52{\~}74\%$의 유사성을 보였고, 그 유전자의 배열 구조도 상이하였다. Comamonas sp. strain DJ-12 is a bacterial isolate capable of degrading of 4-chlorobiphenyl (4CB) as a carbon and energy source. The degradation pathway was characterized as being conducted by consecutive reactions of the meta-degradation of 4CB, hydrolytic dechlorination of 4-chlorobenzoate (4CBA), hydroxylation of 4-hydroxybenzoate, and meta-degradation of protocatechuate to product TCA metabolites. The 6.8 kb fragment from the chromosomal DNA of Comamonas sp. strain DJ-12 included the genes encoding for the meta-degradation of PCA; the genes of protocatechuate 4,5-dioxygenase alpha and beta subunits (pmcA and pmcB), 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (pmcC), 2-pyrone-4,6-dicarboxylate hydrolase (pmcD), 4-oxalomesaconate (OMA) hydratase(pmcE), 4-oxalocitramalate (OCM) aldolase (pmcF), and transporter gene (pmcT). They were organized in the order of pmcT-pmcE-pmcF-pmcD-pmcA-pmcB-pmcC. The amino acid sequences deduced from the nucleotide sequences of pmcABCDEFT genes from Comamonas sp. strain DJ-12 exhibited 94 to $98\%$ homologies with those of Comamonas testosteroni BR6020 and Pseudomonas ochraceae NGJ1, but only 52 to $74\%$ with homologies Sphingomonas paucimobilis SYK-6, Sphingomonas sp. LB126, and Arthrobacter keyseri 12B.

[응모작] 국립남도국악원

강철희(Kang, Cheol-hee) 현대건축사 2001 월간 CONCEPT Vol.- No.23

보문 : 대청호로부터 제작한 메탄지놈 라이브러리에서 1,2-dichloroethane의 분해에 관여하는 dhlA 유전자의 분리

강철희 ( Cheol Hee Kang ),문미숙 ( Mi Sook Moon ),송지숙 ( Ji Sook Song ),이상만 ( Sang Mhan Lee ),김치경 ( Chi Kyung Kim ) 한국하천호수학회(구 한국육수학회) 2005 생태와 환경 Vol.38 No.2

공공임대주택의 커뮤니티 공간 활성화 방안에 관한 연구

정희선(Chung, Hee-Sun),강철희(Kang, Cheol-Hee) 한국실내디자인학회 2019 한국실내디자인학회 학술대회논문집 Vol.21 No.1

Public rental housing is one of the most popular types of housing in Korea. Various attempts are made to change from the quantitative supply of housing to qualitative supply with the aim of housing welfare. The activation of community spaces among the various plans has an important impact on the formation of residential communities and the improvement of habitability. In this study, the current status and problems of our country are identified through theoretical consideration and prior study of public rental housing community facilities. It also investigates and classifies best cases such as foreign countries and proposes the long-term goal of community space in Korea. Although the reality is that the demand for community spaces is rapidly changing, laws and terms are not being correctly defined. The relevant law guarantees autonomy, reviews the space utilization and satisfaction of public rental housing community facilities to determine whether the space should be introduced, and various composition, layout and design elements of interior space suitable for residents" needs will be the main factors for revitalizing the community.

바이오디젤 부산물인 폐글리세롤을 이용한 생물학적 1,3-propanediol 생산

전선애(Sun-Ae Jun),강철희(Cheol-Hee Kang),공성욱(Sean W Kong),상병인(Byoung-In Sang),엄영순(Youngsoon Um) 한국생물공학회 2008 KSBB Journal Vol.23 No.5

자연주의 화장품 브랜드샵에 나타난 표현기법 분석

하지선(Ha, Ji-Seon),강철희(Kang, Cheol-Hee) 한국실내디자인학회 2014 한국실내디자인학회 학술대회논문집 Vol.2014 No.11

Changed lifestyle in modern society shows itself in new consumption behaviors. A rapid shift into the society of economic affluence and competition enhanced the quality of life patterns, and serious environmental problems resulting from the destruction of nature served as a momentum for the emergence of wellbeing culture and the preparation of a framework for its growth. Modern people pursue mentally and physically healthy life, and create environment-friendly activities as well as social regulations for environmental protection. Companies also try to release healthy products, thinking in depth about environment and health. In the cosmetics market as well, natural-ingredient cosmetics, which use the nature and health-centered naturalism concept, have appeared other than synthetic material-centered cosmetics. Hence, this thesis tried to analyze the current situation and spatial expression techniques of brand shops for domestic and foreign naturalism cosmetics brands through examining cases, and to suggest the possibilities of new spaces for future naturalism cosmetics brand shops. Consequently, as for expression techniques that were shown in naturalism brand shops, it was found that there were many cases where natural elements were used directly rather than metaphorically, symbolically or complexly. In addition, it was found that experience spaces, other than selling spaces, for library cafe, powder room, make-up, and esthetics were created, and thus roles as resting space and cultural space were expanding beyond the role of a simple brand shop. Therefore, it is expected from this study that naturalism cosmetics brand shops will develop into spaces of culture and healing through combining more diverse expression elements and a variety of spaces.

자연 친화 개념을 적용한 유치원 공간 계획에 관한 연구

박형근(Park, Hyoung-Keun),강철희(Kang, cheol-hee),나장수(Na, Jang-Soo) 한국실내디자인학회 2017 한국실내디자인학회 학술대회논문집 Vol.2017 No.5

As our standard of living is improving, a generation of children is beginning to pay attention to the natural environment. The interest and expectation toward children is becoming increasingly specialized and specific. It is known that the eco-friendly environment has positive effects on sensibility, intellectual ability, cognitive ability, emotions and expressive ability of children. In this study, it aimed to analyze eco-friendly expressive elements using direct, hybrid and indirect expressive methods, and examine how such features were expressed through case analysis based upon the facts. The space planning of kindergarten should also be changed in order to let even children recognize the value of nature and stay healthy because an eco-friendly space has positive effects on body, mind and sensibility. Further studies should be conducted to support the space planning of kindergarten applied with eco-friendly concept, which can be positively affected even in everyday life, not just a typical, ordinary kindergarten or forest kindergarten regarded as a temporary experience space.

주성분분석과 지구통계법을 이용한 제주도 지하수의 수리지화학 특성 연구

고경석(Kyung-Seok Ko),김용제(Yongje Kim),고동찬(Dong-Chan Koh),이광식,이승구,강철희(Cheol-Hee Kang),성현정(Hyun-Jeong Seong 1),박원배(Won-Bae Park) 대한자원환경지질학회 2005 자원환경지질 Vol.38 No.4

본 연구는 다변량 통계분석을 이용하여 수리지화학적 특성을 분석하고, 주성분 분석을 통해 얻어진 변수를 설명하는 수리지화학적 과정에 대한 해석, 그리고 각 성분과 주성분을 이용한 공간정보에 대하여 지구통계기법을 적용하여 연구지역 지하수의 유동 및 순환 과정을 해석하고자 하였다. 제주도 지하수의 수질에 가장 많이 영향을 미치는 성분은 Cl 과 NO 3 이었으며, 특히 농업활동에 의해 증가되는 NO 3 는 지하수 성분 중 가장 큰 변동을 보여주었다. 다변량 통계분석법인 주성분 분석(PCA)에 의한 수리지화학적 특성 분석 결과, 초기 3 개의 주성분은 전체 분산의 73.9% 를 설명하였다. 주성분 1 은 용존 이온의 증가를 나타내며, 주성분 2 는 탄산염 광물의 용해와 질산염 오염의 영향, 주성분 3 은 양이온 교환반응과 규산염광물의 용해과정을 지시한다. 실험적 반베리오그램 유형 분석 결과 지하수 성분은 크게두 그룹으로 분류되며 각각의 그룹에는 EC, Cl, Na, NO 3 와 HCO 3 , SiO 2 , Ca, Sr 이 속한다. 지하수 성분의 공간분포 특징을 조사한 결과, 전기전도도(EC), Cl, Na 는 해수의 영향을 받는 해안가로 갈수록 증가하는 경향을 보여주며, NO 3 는 농경지의 분포와 밀접한 상관관계를 가진다. 이들 성분은 또한 지형과도 상관성을 가지며 이는 지하수 함양과의 관련성을 나타낸다. 요인 크리깅 수행 결과 PC1 은 Cl, Na, EC 의 공간분포와는 다른 양상을 보여주는데 이는 pH, Ca, Sr, HCO 3 가 PC1 에 미치는 영향 때문인 것으로 분석되었다. 서부지역에서는 PC2 의 이상대가 길게 나타나며 이는 탄산염 광물의 용해와 관련이 있는 것으로 사료된다. 이상의 결과로부터 연구지역 지하수에 대한 다변량 통계분석및 지구통계 분석 기법의 적용은 수질에 대한 복합적 정보의 정량화와 공간 특성을 해석하는데 사용될 수 있다. The purpose of the study is to analyze the hydrogeochemical characteristics by multivariate statistical method, to interpret the hydrogeochemical processes for the new variables calculated from principal components analysis (PCA), and to infer the groundwater flow and circulation mechanism by applying the geostatistical methods for each element and principal component. Chloride and nitrate are the most influencing components for groundwater quality, and the contents of NO 3 increased by the input of agricultural activities show the largest variation. The results of PCA, a multivariate statistical method, show that the first three principal components explain 73.9% of the total variance. PC1 indicates the increase of dissolved ions, PC2 is related with the dissolution of carbonate minerals and nitrate contamination, and PC3 shows the effect of cation exchange process and silicate mineral dissolution. From the results of experimental semivariogram, the components of groundwater are divided into two groups: one group includes electrical conductivity (EC), Cl, Na, and NO 3 , and the other includes HCO 3 , SiO 2 , Ca, and Sr. The results for spatial distribution of groundwater components showed that EC, Cl, and Na increased with approaching the coastal line and nitrate has close relationship with the presence of agricultural land. These components are also correlated with the topographic features reflecting the groundwater recharge effect. The kriging analysis by using principal components shows that PC1 has the different spatial distribution of Cl, Na, and EC, possibly due to the influence of pH, Ca, Sr, and HCO 3 for PC1. It was considered that the linear anomaly zone of PC2 in western area was caused by the dissolution of carbonate mineral. Consequently, the application of multivariate and geostatistical methods for groundwater in the study area is very useful for determining the quantitative analysis of water quality data and the characteristics of spatial distribution.