식품첨가물 안전관리 연구방향

정기화 한국식품위생안전성학회 2009 한국식품위생안전성학회지 Vol.24 No.4

In modern diet relying heavily on processed food, it is difficult to live without food additives, added to food regardless of whether consumers want or not. This poses the following questions: Are food additives really safe? Are they toxic? Are they cancerous? Are they still good to eat? Are there any concerns on excessive intake and interactions? Is toxicity testing adequate? Is ADI evaluation being set up properly? In this article, I discuss a desirable management strategy for food additives, by addressing these questions and concerns and analyzing and reviewing current status of food additive management.

Yeast Two-Hybrid Assay를 이용한 초파리 usp 유전자 산물의 상호결합 파트너 동정

정기화 公州大學校 基礎科學硏究所 1995 自然科學硏究 Vol.4 No.-

초파리 ultraspiracle(usp) 유전자의 산물 (Usp)은 ecdysone receptor (EcR)와 heterodimer를 형성하여 특정 유전자의 발현을 조절하는 스테로이드 호르몬 수용체 superfamily의 한 멤버이다. usp 유전자는 적어도 세가지 중요한 발생기작 즉, 배발생, 눈발생 및 암컷 생식에 관여하는 것으로 알려졌다. 본 연구에서는 세포내에서 단백질-단백질간의 상호결합을 탐지하는데 매우 효과적인 방법인 yeast two-hybrid assay를 이용하여 Usp에 대한 EcR 외 다른 dimerizing partner의 존재 가능성을 초파리 유충 CBNA library의 screen을 통해 조사하였다. 약 5 × 105 cfu의 형질전환체를 screen하여 처음에 32 양성 colonies를 골랐으며, 그 중 3-aminotriazole (3AT)이 첨가된 선별적 결핍 SD 합성 배지에서도 자라는 3개의 양성 line을 최종적으로 선택하였다. The ultraspiracle (usp) gene product (Usp) is a member of the superfamily of steroid hormone receptors in Drosophila melanogaster which mediate the hormone action by heterodimerization with ecdysone receptor (EcR). Based on the genetic and molecular characterization of usp, it has been proposed that Usp functions in at least three significant developmental pathway: embryogenesis, eye morphogenesis, and female reproduction. In the present study, candidators for Usp dimerizing partners other than EcR were identified from Drosophia pGAD larva cDNA library using yeast two-hybrid assay which is very effective method for detection of protein-protein interaction in vivo. From screen of about 5 × 105 cfu, 32 positive colonies were first choosed. But, 3 candidates were finally isolated from 3-aminotriazole (3AT)-contained selective SD synthetic media.

Genotype–phenotype correlation of Charcot-Marie-Tooth type 1E patients with PMP22 mutations

정기화,구혜수,박기덕,최순성,Jin Seok Yu,홍영빈,Ki Wha Chung,최병옥 한국유전학회 2016 Genes & Genomics Vol.38 No.7

Charcot-Marie-Tooth disease type 1E (CMT1E) is a demyelinating motor and sensory neuropathy with peripheral myelin protein 22 (PMP22) point mutations. The objective of this study was to identify genetic causes and determine genotype–phenotype correlation in two Korean demyelinating CMT patients based on whole exome sequencing (WES), histological examination of distal sural nerve, and magnetic resonance imaging (MRI) of leg. WES revealed two de novo PMP22 mutations in the two demyelinating CMT patients, including one novel p.Leu82Pro (c.245T[A) mutation in one patient and one previously reported p.Ser72Leu (c.215C[T) mutation in the other patient. Both mutation sites were located in the well conserved second transmembrane domain. No control had the same mutations. The affected individual with the novel p.Leu82Pro mutation showed early onset, scoliosis, and sensory ataxia with ability to walk without assistance. Histopathological examination showed severe damage of myelin and axons. No compound muscle action potentials (CMAPs) were evoked in the upper or lower limb nerves. Leg MRIs revealed mild fatty infiltration of the bilateral peronei muscles consistent with clinical manifestations. The patient with the p.Ser72Leu mutation showed developmental delay in infancy. No CMAPs were elicited. However, she was also able to walk without assistance. In spite of markedly severe electrophysiological defects, leg MRIs showed almost normal findings except slight muscle atrophies of the lower legs. Both patients presented similar clinical features including no CMAPs in electrophysiological tests and mild fatty replacement in the lower leg MRI. Therefore, there was a good genotype–phenotype correlation in both cases.

Effects of Sperm Motility on In Vitro Production of Embryo and Correlation with Mitochondria Amount in Pig

정기화,In-Cheul Kim,Jung-Ho Son 사단법인 한국동물생명공학회 2010 한국동물생명공학회지 Vol.25 No.4

Prediction of semen's fertilizing ability used in artificial insemination (AI) is one of very important factors on pig reproductive performance. In vitro fertilization (IVF) has been used for indirect evaluation of sperm's fertilizing ability and it has been showed as highly correlated index. In swine industry, increasing interest in preservation of boar semen raises questions on the sperm motility from semen used in commercial AI centers. Mitochondria in sperm mid-piece generate the energy to support motility and could be an explanation of impaired fertility. Objective of this study was to suggest usable sperm motility to farms in measuring the effect of sperm motility and sperm abnormality on in vitro production of embryo in which sperm's fertilizing ability can be determined indirectly. Semen samples were provided from local AI center and used within 3 days after collection. Semen samples were divided by 4 different motile groups (>70%; 61~70%; 51~60%; <50%) using CASA (computer-assisted sperm analysis) on the days of IVF. Developmental rate to the blastocyst stage from over 61% motile sperm group showed significantly higher rate than below 60% motile sperm group (16.5 ± 0.7~18.4 ± 0.8% vs 6.3 ± 0.8~11.5 ± 0.7%, p<0.05). In experiment to determine the relationship between sperm motility and viability and abnormality, over 61% motile sperm groups showed significantly higher viability rate compared to below 60% motile sperm groups (84.8 ± 4.0~88.1 ± 4.0% vs 69.1 ± 4.0~74.2 ±4.0%, p<0.05). On the other hand, morphological sperm abnormality showed significantly higher in over 70% motile sperm group (10.2 ± 2.2 vs 16.0 ± 2.2~21.0 ± 2.2%, p<0.05). In experiment to find the correlation between sperm motility of 4 different motile groups and amount of mitochondria, lower motility group also showed lower level of mitochondria (p<0.05). The mitochondria parameter used in this study showed another possibility to differentiate the sperm motility. Taken together, because below 60% motile semen used in AI reduce the fertility, AI centers should provide the over 60% motile sperm to the farms at the time of AI.

配合藥物의 汲收에 關한 生物 藥劑學的 硏究 (II) : Salicylamide에 Scopolamine 과 Chlorpromazine을 配合投與하였을 때의 鎭痛效果에 關하여

金在完,鄭淇化 德成女子大學校 1972 德成女大論文集 Vol.1 No.-

Comparative studies were made on the analgesic effect of salicylamide used individually and combined with parasympatholytic agent (scopolamine) and tranquilizer (chlopromazine) as regards the analggesic effect (in Cucinell's method) were examined by three assumption cross-over test to experimental groups (mouse), and the following effect were found. 1, The active intensity of salcylamide(S.A.) by its oral administration with cmombined scopolamine (S.C.) is as follows: 1:1>2:1>1:2(S.A.:S.C.) and order of durability is as follows: 1:2>2:1>1:1(S.A.:S.C.) 2, The active intensity chlorpromazine (C.P.) by its oral administration with combined chlorpormazine is as follows: 2:1>1:1>1:2>(S.A.:C.P.)

음식물 쓰레기의 발효ㆍ건조를 이용한 사료 및 퇴비화

정기화 공주대학교 자원재활용신소재지역협력센터 2000 2차년도 센터 사업 성과집 Vol.2000 No.1

영아에서 발생한 비순구의 결절성 근막염 1례

정기화,장태영,김영효,김루시아 대한비과학회 2011 Journal of rhinology Vol.18 No.2

Nodular fasciitis is a benign proliferative spindle-cell lesion that presents as a rapidly growing mass with rich cellularity and mitotic activity, leading to its frequent misidentification of a sarcoma. Most cases of nodular fasciitis are diagnosed in adults aged 20-40 years and is rarely seen during the first 4-5 years of life. Because of its rapid growth and bony erosion, it often mi-mics malignancy. We report a case of an 18-month-old infant with a rapidly growing mass on the right nasolabial fold. Because of the anatomic location and CT findings, we first suspected nasolabial cyst. However, it proved to be an intramuscular type of nodular fasciitis, a very rare occurrence in infancy.

유해물질 관리정책의 과학화

정기화 한국식품위생안전성학회 2011 한국식품위생안전성학회지 Vol.26 No.2

Though food safety efforts of the government has been increasing, but the health hazards resulting from food does not stop and thus increases consumers' anxiety and distrust of government. For more reliable safety management, developing analysis and intake measurement methods and strengthening regulatory control with strict criteria is necessary. In addition, as not only the known hazards by now, but also unknown but obvious hazards are simultaneously ongoing in current environments, the unified risk management and health assessment for food, health food and medicines from the media is critical. As risks and damage to health may show up after a long time after exposure,preventive measures and strategies for safety control is also needed.

低開發의 接近方法에 대한 -考察 : -近代經濟學과 政治經濟學-

鄭奇和 全南大學校 社會科學硏究所 1982 社會科學論叢 Vol.10 No.-

A Large Dominant Myotonia Congenita Family with a V1293I Mutation in SCN4A

정기화,유다혜,이수정,최병옥,이상수 대한신경과학회 2016 Journal of Clinical Neurology Vol.12 No.4