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RISS 인기검색어
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- 저자 : 柳點鎬 (검색결과 65 건)
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류점호,조천준,한철수,류익상 한국연초학회 1984 한국연초학회지 Vol.6 No.2
This experiment was carried out to select the suitable burley varieties for paddy field. Two varieties, two $F_1$ hybrids and three $F_1$ hybrids with pale yellow line which were all early maturing were tested in the paddy field condition. The results were as follows. 1. Va 528 and all $F_1$ hybrids flowered 4-8 days earlier than Burley 21. 2. Leaf maturity among varieties and $F_1$ hybrids were not significantly different at first priming, but significant differences were detected from second to forth primings. (MS Ky 12x JB 7802-30)Fl and (MS Br 21 x L8)$F_1$ hybrids matured 3-5 days earlier than Burley 21. Pale yellow hyorids matured early, yielded high and were favorable in physical properties, but were low in quality. 4. Va 528 and (MS Br 21 x L8) $F_1$ hybrid showed earthy maturing, high yield and good quality compared with Burley 21. So two varieties were considered suitable for paddy field cultivation.
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종자의 성숙도가 Burley종 담배의 발아에 미치는 영향
柳點鎬,裵成國,韓喆洙,秋洪求 韓國作物學會 1983 한국작물학회지 Vol.28 No.2
본 시험은 발아가능한 담배종자를 조기 파종하여 파종에 이용하고자 몇가지 버어리종 담배를 공시하여 수분후 8일부터 30일까지 2일간격으로 채종 발아조사를 하였던 바 1. 종자는 수분후 12일 이상이면 발아가능하여 파종에 이용할 수 있었고 발아율은 낮았으며 품종에 따라 변이가 컸다. 2. 적기채종시기는 수분후 24-28일이였다. 3. 과숙종자는 후숙이나 일시휴면현상을 보여 발아율이 감소하였다. This experiment was conducted to evaluate the effect of early seed harvest on germination of tobacco(Nicotiana tabacum L.) seeds. Seeds of seven burley tobacco were harvested every two days from 8 to 30 days after pollination and tested for germination. The results are; 1. Seeds harvested 12days after pollination germinated but germination rates were low and varied across cultivars. These seeds will provide viable seeds adequate for breeding program. 2. Germination rates of seeds harvested 24-28 days after pollination were high and showed none significant differences among cultivars. 4. Over-ripen seeds showed low germination rates cused by after-harvest or temporary dormancy.
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류점호 한국식물생명공학회 1997 식물생명공학회지 Vol.24 No.6
알팔파의 자엽과 하배축을 배양하여 캘러스를 유기하고 이들을 glyphosate첨가 액체배지에서 배양하여 glyphosate에 대한 생장억제 반응을 검사하였으며 10 mM glyphosate에 저항성인 세포주를 선발하고 EPSPS 활성을 조사 하였던 바 캘러스 유기율은 2,4-D 1 mg/L, kinetin 0.5 mg/L에서 가장 높았고 자엽보다 하배축이 캘러스 유기율이 높았으며, glyphosate 농도에 대한 ID$_{50}$은 0.1 mM과 0.2 mM사이에 있었다. A49-10G와 A58-10G는 대조세포주에 비해 각각 8.0배 와 9.1배의 높은 EPSPS 활성을 보였다. Calli were induced from the cotyledon and the hypocotyl of alfalfa and the callus lines were tested for the resistance to glyphosate in the liquid medium containing 0.01-3.00 mM glyphosate. Some resistant cell lines were selected from the gradual increase of glyphosate concentration and the lines resistant to 10 mM glyphosate were analyzed with EPSPS activity. Vigorous callus proliferation from the cotyledon and the hypocotyl was observed from the MS medium containing 1 mg/L 2,4-D and 0.5 mg/L kinetin. The hypocotyl was thought to be better explant source for callus induction than the cotyledon. $ID_{50}$ (Inhibition Dosage of 50%) to glyphosate was between 0.1 mM and 0.2 mM level. A49-10G and A58-10G cell lines selected as resistant to 10 mM glyphosate had 8.0 and 9.1 fold increased EPSPS activity to those of the control lines, respectively.
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백두산지역과 국내 더덕 수집종의 RAPD에 의한 유연관계 분석
두홍수,류점호,이강수,이호림,유헌호 한국약용작물학회 2002 한국약용작물학회지 Vol.10 No.3
한국과 중국 동북부 지역에서 수집한 16종의 더덕으로부터 genomic DNA를 추출한 후, Bioneer사로부터 구입한 random primer(10-mer)를 이용하여 PCR을 수행하였다. 총 49종의 primer를 스크린 한 결과 재현성이 있으면서 polymorphism을 보이는 20개의 primer를 선발하였다. 선발한 primer로부터 PCR에 의해 증폭된 DNA의 크기는 125 bp에서 2.0 kb 내외였으며, 총 148개의 band가 관찰되어 평균 band 수는 7.4개였다. 이들 중에서 polymorphism을 보이는 band의 수는 73개이었으며 (49.3%), polymorphism은 각 primer별로 1~9개로써 다양하였다. 16개 수집종의 유사계수 범위는 0.682에서 0.959로써 유전적 유연정도는 크지 않았다. UPGMA 분석에 의한 수집종들의 유연관계를 dendrogram으로 나타낸 바, 수집종들간의 유전적 거리는 0.133~0.400이었으며, 국내 수집종과 중국 수집종간에는 확실하게 두 그룹으로 분류되었고, 유전적 거리는 약 0.281이었으며, 이들은 모두 지역적인 차이를 보였다. 한편, 중국의 '통화현(通化縣)'과 '류하현(柳河縣)' 수집종이 다른 지역의 수집종보다 유전적 거리가 가장 크게 나타났다. Extracted genomic DNA from 16 accessions of Codonopsis lanceolata collected from South Korea and the Baekdoo Mt. areas of China were analyzed for their genetic relationships by RAPD. Twenty 10-mer-oligonucleotide primers having reproductive polymorphism were selected for the RAPD analysis. The size of amplified DNA was almost between 125 bp and 2.0 kbp. Sixteen collected Codonopsis lanceolata were analyzed with 20 primers which generated 73(49.3%) polymorphic bands among 148 PCR products. The mean number of polymorphic bands were 7.4 and varied 1~9 per primer. It was, thus, demonstrated that RAPD was useful for detecting polymorphism in Codonopsis lanceolata. The range of 1-F value(genetic similarity) was from 0.682 to 0.959. These results indicate variable genetic similarities. By UPGMA (Unweighted Pair Group Method using an Arithmetic average) cluster analysis based on 1-F value, genetic distance among the 16 collected Codonopsis lanceolata was 0.133~0.400. It was certainly classified into two groups between collected accessions from Korea and China, and the genetic distance was about 0.281. Both accessions collected from Korea and China showed miner differences, while the genetic relationships of Tonghua Xian and Liuhe Xian from China was farthest with other accessions collected.
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시설재배 참외의 단위결과에 미치는 식물생장조절제의 영향
권태호,류점호,최선영,추병길,강천식 한국국제농업개발학회 2001 韓國國際農業開發學會誌 Vol.13 No.3
본 실험은 참외에서 단위결과 유기에 적합한 식물생장조절제의 영향을 규명하기 위해 수행되었다. 오옥신계 식물생장조절제인 4-CPA, 2,4-D, NAA, IAA를 농도별 단독으로 처리했으며 자방에 침투가 잘되도록 2mM DMSO와 2% tween-20액을 첨가했고 모든 실험은 무가온 PET하우스내에서 실시하였다. 개화기부터 평균수확소요일은 NAA 처리구에서 29일로 가장 짧았고 인공수분구는 31일 4-CPA처리구는 33일로 가장 길었다. 4-CPA와 2,4-D처리구에서의 과실 착과율은 인공수분구에 비해 높았지만, 과실수확율은 인공수분구에 비해 4-CPA 30ppm 처리구에서만 약간 높았으며 NAA와 IAA 처리구에서는 착과율과 수확율 모두 현저하게 낮았으며 무처리구에서는 과실수확을 할 수 없었다. 열과는 모든 처리구에서 발생했으며 NAA처리구가 특히 높았고, 과중 또한 NAA 처리구가 훨씬 무거웠다. 당도는 인공수분구와 NAA 처리구가 약간 낮았고, 식물생장조절제 처리 부위는 화경에 비해 자방이 훨씬 효과적이었다. This experiment was carried out to understand the effect of plant growth regulators on parthenocarpy occurs in oriental melon. Used plant growth regulators were 2,4-D, NAA, IAA and 4-CPA and treated individually. 2mM DMSO and 2% tween-20 have added so that permeation may go well to ovary in each plant growth regulator. The experiment was performed to under greenhouse condition. The results are as follows : NAA treatments of harvesting need period were short by 29 day, 4-CPA treatments weres longest by 33 day. 4-CPA and 2,4-D of fruit set ratio were high about 10-15% than pollination. Plant growth regulators did not increase the harvested fruit rate compare to the pollination except for 4-CPA 30 ppm which showed 57%. In general, plant growth regulator treatments significantly increased average fruit weight except for 4-CPA treatments as compared with pollination, the highest one being 759g with NAA 1mM treatment. Cracking have happened in all treatments that include pollination and the occurrence rate was high in NAA treatment specially. Flesh thickness expressed fruit fresh weight and tendency that is proportional. It was most effective that 4-CPA treatment to ovary and plant growth regulators processing of sugar content was some higher than pollination, except NAA treatments.
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