학술발표초록 ; 육종 ( 구두발표 ) ; 가금류의 정자 세포 배수성 유기를 위한 세포유전학적 연구

손시환,김철욱 한국동물자원과학회 ( 구 한국축산학회 ) 1996 한국축산학회 심포지움자료집 Vol.- No.-

혈액세포를 이용한 염색체 분리 분석에 관한 방법적 고찰

손시환,정구민,Sohn, S.H.,Chung, K.M. 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.2

This study was carried out to develop the methodology of chromosome preparation from blood cultures in mammals which included human, mouse, cattle and pig. For karyotyping, 0.5-5.0ml of peripheral blood were collected and cultured. The satisfactory results were obtained from macroculture and microculture in all species. In culture, the patterns of cell growth were no difference among media except serum concentration and mitogen supplement. The presence of mitogen and fetal bovine serum in medium significantly affected the mitotic index. The optimal culture condition was 37$^{\circ}C$ for 3 days. And the concentration of colcemid and reincubation time also affected the chromosome morphology. In harvest, chromosome patterns were mainly affected on hypotonic treatment which included treated time and temperature, dropwise of fixative solution, and drying after slide preparation.

가금육종의 변천과 종계산업의 발전방향

손시환 대한양계협회 1991 월간 양계 Vol.23 No.10

乳牛에 있어서 産乳能力에 影響을 미치는 空始期效果에 관한 硏究

池卨夏,申英秀,李學敎,高文石,吳鳳國,孫始煥 서울대학교농과대학농업개발연구소 1988 서울대농학연구지 Vol.13 No.2

This study was carried out to estimate relationships between days open and milk yield at 305 day and according to season of freshing. Data used for this study included a total of 3,266 sets of 305day lactation records of Holstein cows tested milk yield performance in National Animal Breeding Institute from 1971 to 1987. Parity, year at calving and days open showed highly significant (p<0.01) effects on actual and age-corrected milk yields in the first season of refreshing. Days open affected on age-corrected milk yield significantly (p<0.05) and parity and year at calving also effected significanty (p<0.01) on milk record in the second season of refreshing. As days open increased from 20 to 200days, actual and age-corrected milk yield increased from 4,998kg and 5,415kg to 5,290kg and 5,702kg in the frist season, respectively. In the second season, actual and age-corrected milk yields were also affected by period of days open. (Key words : Days open, milk yield)

한국재래돼지의 G-, C-, 및 NOR-banding

손시환,권오섭,백규흠,정원,조은정,강민영 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.6

Using the G-, C-, and NOR-banding techniques, a karyotyping for Korean native Pig was performed. Blood samples were collected from 50 male Korean Native Pigs that had been bred at the National Livestock Research Institute and then blood cells were prepared from in vitro cultures followed by karyotyping ; G-, C-, and NOR-banding patterns of metaphase chromosomes were analyzed. The karyotype of Korean Native Pig is 38, XX or XY which consists of 5 pairs of submetacentric chromosomes(Group Ⅰ), 2 pairs of acrocentric chromosomes with short p-arm(Group Ⅱ), 5 pairs of medium metacentric chromosomes(Group Ⅲ), 6 pairs of acrocentic chromosomes(Group Ⅳ) and metacentric X and Y sex chromosomes. On GTG-banding, the Korean Native Pig exhibited a typical and identical banding pattern in each homologous chromosomes. Overall chromosomal morphology and positions of typical landmarks of the Koreans Native Pig were virtually identical chromosomal morphology and positions of typical landmarks of the Korea Native Pig were virtually identical to those of Committee for the Standardized Karyotype of the Domestic Pig(CSKDP). However, numbers of G-bands of the Korean Native Pig chromosomes were more than those of CSKDP. In chromosomes 1, 3, 5, 6, 7, 8, 13, 14, 15, 16, 17, 18 and X, the Korean Native Pig exhibited more separated bands as compared with CSKDP. In C-banding patterns, although the quantity of heterochromatin was variable in each chromosome, most of the Korean Native Pig chromosomes had heterochromatic C-bands on centromeres. However, the heterochromatic C-band was constantly observed on the whole Y chromosome. In AgNOR staining, the NORs were located at centromeres on the chromosomes 8 and 10. The number of NORs per metaphase ranged from 2 to 4 giving a mean value of 2.13. The number of NORs wer distributed on all chromosome pair 10 but not on chromosome 8. The sizes of NORs were also differed between homologous chromosomes 8. Numbers of NORs of Korean Native Pig were significantly higher than those of Yorkshire. The pattern of pig NORs was polymorphic in breeds, individuals and cells, especially on chromosome 8.

닭(Gallus domesticus) 染色體의 數 및 形態的 考察

孫始煥,吳鳳國 서울대학교 농과대학 실험목장 1988 서울대학교 농과 대학 부속 실험 목장 보고서 Vol.1 No.1

닭 초기배자로부터 염색체 분리기법의 개발 : Ethidium bromide 를 이용한 고정도 염색체의 유도 Induction of High - resolution Chromosomes by Using Ethidium Bromide

손시환,류은경,백규홈 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.2

At the time of cell division, chromatin becomes condensed into the chromosomes. Accordingly, for chromosome analysis, an inhibitor of spindle fiber such as colchicine is commonly added to the cell culture to restrain the cell division at metaphase. Because the prophase chromosomes are longer and less condensed than metaphase chromosomes, the prophase chromosomes are much more useful than metaphase ones for high-resolution karyotyping. The present study was conducted to investigate the effect of adding ethidium bromide (EtBr), an inhibitor of chromatin condensation, into chick embryo culture on the induction of prophase chromosomes. Injection of 1 ㎍ EtBr into the air sac of chick embryo at the end of the culture resulted in an increase in the ratio of prophase chromosomes, indicating that chromatin condensation was effectively inhibited by this reagent. Additions) treatment of the culture with 100 ㎍ colchicine following the EtBr improved the gross morphology of the chromosomes, with no adverse effect on the number of prophase chromosomes. Moreover, the relative length and number of G-bands of macrochromosomes at prophase were 1.7-2.0 times as long as and about 2 times as great as those at metaphase, respectively. As the EtBr was highly effective in inhibiting the condensation of chick chromosomes, we propose the usage of EtBr as an improved method for preparing high-resolution chromosomes for the chick embryos.

닭 染色體의 分離 分析 方法에 관한 硏究

孫始煥,吳鳳國 서울대학교 농과대학 실험목장 1988 서울대학교 농과 대학 부속 실험 목장 보고서 Vol.1 No.1

양계업 경쟁력 강화 방안 - 계란품질에 미치는 요인

손시환,Son, Si-Hwan 대한양계협회 2015 월간 양계 Vol.47 No.8

토종 종계 육성기 체중이 산란 능력에 미치는 영향

손시환,김기곤,최은식,오상현 한국가금학회 2023 韓國家禽學會誌 Vol.50 No.1

This study was to investigate the relationship between chick weight in the growing period and the layingperformance of Korean native chicken (KNC) breeders. We measured the body weights of 1,384 KNC breeders at 1-140 daysof age at two-week intervals. The age at first egg laying, hen-day and hen-housed egg production, and the egg weights wereinvestigated. Correlation coefficients between the body weight and egg production traits were analyzed. The results showedthat the correlation coefficients between body weight and age at first egg laying were significantly positive before 4 weeks,but negative after 8 weeks of age. The correlation coefficients between body weight and egg production rate were estimatedto be negative before 8 weeks and positive after 12 weeks of age. Coefficients of hen-day and hen-housed egg productionwere similar. There was little correlation between body weight and egg weight at 32 weeks of age, whereas a low positivecorrelation was with egg weight at 50 weeks. In conclusion, there was little correlation between body weight and layingperformance before 8 weeks of age, whereas a significant positive correlation was with body weight after 12 weeks. Theresults suggest that as the chick's weight increases in the late growing stage, sexual maturity is faster, the egg productionrate is higher, and the egg weight increases. Therefore, the weight management of KNC female breeders is very importantin the late growing stage, and the body weight of chicks must be maintained above the standard weight 본 연구는 토종 종계의 육성기 체중과 산란 능력 간의 연관성을 규명하고자 성숙 이전 주령별 체중과 이들의 초산일령, 산란율 및 난중과의 상관 관계를 분석하였다. 공시계는㈜한협원종이 보유한 신품종 토종 종계 1,384수로써 발생부터 20주령까지의 체중과 초산일령, 초산이후 50주령까지의일계 산란율 및 산란지수, 32주령 및 50주령 난중을 분석 대상으로 하였다. 분석 결과, 육성 초기 체중과 초산일령 간에는 정(positive)의 상관이, 육성 후기 체중과 초산일령 간에는 부(negative)의 상관이 나타났다. 육성기 체중과 일계 산란율 및 산란지수 간에는 비슷한 상관계수를 보이고, 육성전기 체중과 산란율 간에는 부의 상관이, 육성 후기 체중과는 정의 상관을 보였다. 육성기 체중과 32주령 난중 간에는거의 상관 관계가 없는 것으로 나타났고, 50주령 난중과는 낮은 정의 상관을 보였다. 결론적으로 토종 종계에 있어서 8주령 이전까지의 육성 초기 체중과 산란 능력 간에는 거의 연관성이 없는 것으로 보여지고, 12주령 이후 육성 후기 체중과 산란 능력 간에는 정의 상관이 있는 것으로 나타났다. 이는 육성 후기 때 병아리의 체중이 증가할수록 성 성숙이 빠르고, 산란율도 높고 난중도 증가함을 시사한다. 따라서 토종 종계 모계의 경우 육성 후기 병아리의 체중 관리가 매우 중요하고, 이때 종계들의 체중이 표준 체중 이상이 되도록 유지하여야 한다.