고분염분석법(High - resolution banding)에 의한 한국재래계의 염색체 분염 표지 설정

백규흠,손시환 한국가금학회 2000 한국가금학회 정기총회 및 학술발표회 Vol.17 No.-

한국재래계의 염색체 분염 표지 분석

백규흠,이철영,상병돈,최철환,김학규,손시환 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.1

본 연구에서는 국내 고유 재래 가축들의 유전정보와 유전학적 개량의 기초자료 제공을 목적으로 고분염 분석(high-resolution banding) 방법에 의한 한국재래계의 염색체 분염 표지를 제시하였다. 본 연구에 공시된 한국재래계로서의 축산기술연구소에서 계통화 시킨 황갈색 및 적갈색계통으로 이들이 생산한 수정란의 초기 배자를 이용하여 염색체 분석을 수행하였으며 닭의 초기배자에 EtBr 및 colchicine을 처리함으로써 보다 양호한 고정도 염색체를 획득하였다. 한국 재래계의 GTG-banding 결과 모든 상동염색체간에 뚜렷하고 특징적인 band 양상을 얻을 수 있었으며, Leghorn 및 국제표준핵형(ISSAK)과 비교시 염색체의 형태적 양상에서는 거의 차이가 없는 것으로 분석되었고 대표적 landmark간에도 거의 일치되는 양상을 보였다. 그러나 대부분의 한국재래계의 대형염색체에서 더 많은 G-band의 분리 양상을 보이고 특히 1번 및 Z염색체에서 특징적 분리 양상의 차이를 보였다. 한국재래계의 C-banding 분석에서는 세포별 heterochromatin의 다형성을 보이기는 하나 대부분의 염색체의 동원체와 말단부위에서 C-band가 나타났으며, Z 염색체 장완 말단부와 W 염색체 전체에서는 거의 모든 세포에서 C-band가 출현하였다. 또한 3번 염색체 동원체와 Z 염색체 장완 말단부에서 특징적 다형성을 나타내어 이들 염색체들에서는 상동염색체간 heterochromatin의 이형적 양상(heteromorphic)이 존재함을 밝혔다. The present study was carried out to establish the standard karyotype of the Korean Native Chicken and to find their chromosomal band markers using high-resolution banding technique. Chromosome analysis was performed on early chick embryos following in vitro culture of fertilized eggs of the yellow-brown and the red-brown lines of the Korean Native Chicken which had been established at National Livestock Research Institute. The high-resolution banding of the chromosome was achieved by treating the embryos with ethidium bromide and colchicine during culture. On GTG-banding, the Korean Native Chicken exhibited a typical chick banding pattern in all the macrochromosomes. Overall chromosomal morphology and positions of typical landmarks of the Korean Native Chicken were virtually identical to those of White Leghorn International System for Standardized Avian Karyotypes(ISSAK0. However, the lengths and G-band number of the Korean Native Chicken macrochromosomes were greater than those of White Leghorn and ISSAK. Especially in chromosomes I and Z. the Korean Native Chicken exhibited more separated bands in compared with ISSAK. In C-banding patterns, although a lot of observed cells had C-band polymorphic patterns. almost the Korean Native Chicken macrochromosomes had heterochromatic C-band on centromeres and/or near terminal part. However, the heterochromatic C-band was constantly observed at the end of q-arm of Z chromosomes and on the whole W chromosome. In addition, the Korean Native Chicken exhibited distinctive heteromorphic patterns of C-bands on the centromere of chromosome 3 and at the end of q-arm of Z chromosome between homologous chromosomes.

경영환경과 경영전략 및 경영혁신이 경영성과에 미치는 영향에 관한 연구

백규흠 한국전문경영인학회 2017 專門經營人硏究 Vol.20 No.3

The purpose of this research is to comprehensively analyze the usage and properties of management environment, management strategy, and management innovation on cause-and-effect relationship and management performance. To achieve the research aim, a hypothesis was set through a theoretical consideration as well as the results and the implications of previous studies, and a research model and variables were established. Therefore, this study seeks to identify the structural influence of management environment, management strategy, and management innovation on management performance. To do so, first, the study will arrange the theoretical foundation of management environment, management strategy, management innovation, and management performance; and second, it will review the structural model among management environment, management strategy, management innovation, management performance. In conclusion, it is expected that the results of this research will innovate the organizations of small and medium-sized manufacturing companies that have difficulties in responding to management environment, due to market uncertainty and insufficient material and human resources in an intense market competition. By doing so, the results will make positive contribution to the improvement of management performance by using a management innovation technique through management strategy.

Relationships of Plasma Insulin-like Growth Factor (IGF)-Ⅰ and IGF-ⅡConcentrations to Litter Size and Lactation Performance in Landrace and Yorkshire Pigs

Lee, C. Y.,Baik, K. H.,Lee, D. H.,Park, H. C. 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.1

본 연구는 혈장의 insulin-like growth factor(IGF)-Ⅰ과 IGF-Ⅱ 농도와 산자수 및 비유성적과의 관계를 구명하고자 착수되었다. 분만주가 비슷한 미경산 및 경산 임신돈 중에서 선발한 총 60두의 순종 Landrace와 Yorkshire 공시돈을 저산자수 (low; <μ - 0.5 SD)와 고산자수 (high; >μ + 0.5 SD)-lines로 구분하여 2 (품종) × 2(line) 요인분석 실험설계 하에 본 실험을 진행 하였다. 공시돈은 분만시 모돈당 포유자돈수를 9두로 고정시키고, 3주령 이유시 비유량의 척도로서 총 이유자돈 체중을 측정하였다. 혈액시료는 임신 90일과 분만 후 15일에 경정맥으로부터 채취하였다. 본 실험기간 중 산자수 및 본 실험기간까지의 평균 산자수는 high-line이 low-line보다 각각 3.7두와 2.4두 많았고 (P<0.01), 두 품종간 산자수 차이는 없었다. 임신 90일 혈장의 IGF-Ⅱ 농도는 low-line보다 high-line에서 높았다. 산자수는 임신 90일 IGF-Ⅰ 농도와 Landrace에서는 부의 상관관계를 나타냈고 (r=-0.46; P<0.05), Yorkshire에서는 부의 상관관계 경향을 나타내어 (r=-0.31; P=0.09) 전체 공시돈에서는 이들 두 변수간 유의적인 부의 상관관계를 보였다 (r=-0.35; P< 0.01). 이유시 모돈당 총 이유자돈 체중은 두 품종간 혹은 두 lines간 차이가 없었다. 이유자돈 체중과 IGF 농도와의 관계는 품종 × 생리적인 생태 조합들간 일정한 경향을 나타내지 않았다. 이상의 결과는 임신 90일 IGF 농도가 임박한 분만시 산자수의 지표가 될 수 있는 가능성을 시사한다. The present study was undertaken to find relationships of plasma insulin-like growth factor (IGF)-Ⅰand IGF-Ⅱ concentrations to litter size and lactation performance. Sixty pure-bred Landrace and Yorkshire pigs having similar farrowing weeks which had been selected from a large number of pregnant gilts and sows were divided into low- (<?? - 0.5SD) and high-litter size (>?? + 0.5 SD) lines under a 2 (breed) × 2 (line) factorial arrange of treatments. After adjusting the litter size to nine piglets per sow at farrowing, total litter weight was measured at three weeks postpartum at weaning as an index of milk yield. Blood samples were obtained from the jugular vein at day (d)-90 pregnancy (Px) and at d-15 postpartum. The litter size or the number of piglets born during the present experiment and the average litter size during the entire parities up to the present one were greater in the high-line than in the low-line by 3.7 and 2.4 piglets. respectively (P<0.01); effect of the breed on litter size was not significant. Plasma IGF-Ⅱ concentration at d-90 Px was greater in the high-line than in the low-line. Litter size and d-90 Px IGF-Ⅰconcentration were negatively correlated in Landrace (r=-0.46; P<0.05) and tended to be negatively correlated in Yokshire (r=-0.31; P=0.09), which resulted in a significant negative correlation between these two variables in total animals (r=-0.35; P<0.01). Litter weight at weaning was not different between the two breeds or lines. Relationships between the litter weight and IGF concentration were not consistent across the breed × physiological stage combinations. Results suggest that d-90 Px IGF concentrations may be indicative of the litter size at impending farrowing.

한국재래돼지의 G-, C-, 및 NOR-banding

손시환,권오섭,백규흠,정원,조은정,강민영 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.6

Using the G-, C-, and NOR-banding techniques, a karyotyping for Korean native Pig was performed. Blood samples were collected from 50 male Korean Native Pigs that had been bred at the National Livestock Research Institute and then blood cells were prepared from in vitro cultures followed by karyotyping ; G-, C-, and NOR-banding patterns of metaphase chromosomes were analyzed. The karyotype of Korean Native Pig is 38, XX or XY which consists of 5 pairs of submetacentric chromosomes(Group Ⅰ), 2 pairs of acrocentric chromosomes with short p-arm(Group Ⅱ), 5 pairs of medium metacentric chromosomes(Group Ⅲ), 6 pairs of acrocentic chromosomes(Group Ⅳ) and metacentric X and Y sex chromosomes. On GTG-banding, the Korean Native Pig exhibited a typical and identical banding pattern in each homologous chromosomes. Overall chromosomal morphology and positions of typical landmarks of the Koreans Native Pig were virtually identical chromosomal morphology and positions of typical landmarks of the Korea Native Pig were virtually identical to those of Committee for the Standardized Karyotype of the Domestic Pig(CSKDP). However, numbers of G-bands of the Korean Native Pig chromosomes were more than those of CSKDP. In chromosomes 1, 3, 5, 6, 7, 8, 13, 14, 15, 16, 17, 18 and X, the Korean Native Pig exhibited more separated bands as compared with CSKDP. In C-banding patterns, although the quantity of heterochromatin was variable in each chromosome, most of the Korean Native Pig chromosomes had heterochromatic C-bands on centromeres. However, the heterochromatic C-band was constantly observed on the whole Y chromosome. In AgNOR staining, the NORs were located at centromeres on the chromosomes 8 and 10. The number of NORs per metaphase ranged from 2 to 4 giving a mean value of 2.13. The number of NORs wer distributed on all chromosome pair 10 but not on chromosome 8. The sizes of NORs were also differed between homologous chromosomes 8. Numbers of NORs of Korean Native Pig were significantly higher than those of Yorkshire. The pattern of pig NORs was polymorphic in breeds, individuals and cells, especially on chromosome 8.

거세비육돈에서 성선스테로이드호르몬이 성장효율, 도체 특성 및 혈중 Insulin-like Growth Factor(IGF)-1 및 IGF-Binding Protein-3 농도에 미치는 영향

이철영,하승호,이희포,백규흠,진상근,손시환,박만종 한국동물생명공학회(구 한국동물번식학회) 2005 Reproductive & Developmental Biology(Supplement) Vol.29 No.2s