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가시오갈피와 두충의 첨가 급여가 브로일러의 생산 능력, 혈장 생화학 지표 및 텔로미어 함량에 미치는 영향
손시환,장인석,문양수,김영주,이수희,고영현,강선영,강혜경 한국가금학회 2008 한국가금학회 정기총회 및 학술발표회 Vol.25 No.-
An experiment was conducted to investigate the effect of Siberian ginseng leaf and Eucommia leaf at level of 0.5% and 1% per feed in Ross commercial broiler for 4 to 35 days of age on performance, organ weight, blood biochemical profiles and telomere quantity. Chickens consuming diets containing 1% Siberian ginseng had higher feed conversion ratio than the other treated chicken during experimental period whereas no significant differences were detected in body weight, weight gain and feed intake. The weight of bursa of fabricius was significantly increased in chickens with dietary supplementation compared with chickens fed control but this was not seen in liver, spleen and thymus. In blood biochemical profiles, chickens with dietary supplementation had higher concentration than chickens fed control in triglyceride, cholesterol and glucose. The concentration of aspartate aminotransferase, alanine aminotransferase, albumin and total protein, however, was not significantly different between dietary supplemented chickens and control chickens. The relative amount of telomeric DNA of lymphocytes in chickens with dietary supplementation was significantly higher than that of control chickens but the difference was not found in liver, heart and testis tissues. In conclusion, dietary supplementation of Siberian ginseng and Eucommia in broiler improved immune activity and telomere length without decreasing chicken growth performance.
일본산메추리(Coturnix coturnix japonica)의 염색체 다형현상
손시환 한국가금학회 1990 韓國家禽學會誌 Vol.17 No.4
Constitutive heterochromatin의 염색체 다형현상에 대해 사람을 비롯하여 돼지, 생쥐, 말, 닭 등에서 보고된 바 있다. 본 연구에서는 일본산메추리의 C-band 다형체 뿐만 아니라 염색체의 형태적 다형체를 발견하여 이의 다형현상을 밝혔다. 일반적인 염색체 분석방법 및 C-banding 방법으로서 밝힌 3가지 염색체 다형체는 4번 염색체 +/- 동형체, +/- 이형체 및 -/- 동형체이다. 이와 같은 다형체들은 무작위 집단 내에서 일반적이고 지속적으로 나타나며 Mendel 법칙에 따른 유전양상을 보인다. 따라서 본 연구에서 밝힌 염색체 다형체들은 여러 세포유전학적 연구에 표식인자(chromosome marker)로서 유용하게 이용되어질 수 있을 것으로 생각된다. Comosomal polymorphism involving constitutive heterochromatin has been reported in mu, pigs, mouse, horse, chicken and so on. The chromosomal polymorphism of Japanese quail which includes constitutive heterochromatin as well the chromosomes without banding treatment has now been found. Through the use of a general technique that permits visualization of chromosome morphology and heterochromatin, three chromosomal variants have been found among birds ; +/+ homozygous from, +/- heterozygous form and -/- homozygous form in chromosome 4. This variants appear to be common in the randombred population and stably inherited in a Mendelian fashion. These results suggest that the variants would be useful as chromosomal markers for various types of cytogenetic studies.
일본산 메추리(Coturnix coturnix japonica)의 핵형연구
손시환,이경희,N. S. Fechheimerlr 한국가금학회 1990 韓國家禽學會誌 Vol.17 No.4
일본산메추리(Coturnix coturnix japonica)의 핵형연구를 위하여 정소조직 및 백혈구, 초기태아조직들로서 염색체 분석을 수행하였다. 이들 염색체의 수는 정상 이배체(2n)가 78개로 나타나고, 계2 감수분열 중기상태의 정모세포의 관찰에서 반수체(n)가 39개로 나타났다. 한편 8개의 대형염색체 및 성 염상체에 대한 형태적 분석은 동원체지수 및 상대적 길이비로서 측정하였다. 이용된 여러 조직간의 염색체의 형태적 차이는 거의 없는 것으로 나타났으나 4번 염색체템서 p-alm의 존재유무에 따라 상당한 변이를 나타내었다. Chromosome complements of Japanese quail (Coturnix coturnix japonica) were studied using several tissues which involving testis, leukocytes and embryos. The diploid count was estimated to be 2n=78. Analyzing the metaphase of secondary meiosis in spermatocytes, the haploid count estimated to be n=39. Morphometric analysis were studied by the centromeric index and relative length of 8 macro-chromosomes and Z, W chromosomes The differences of morphological feature were not significant among tissues. Exceptionally the chromosome 4 shelved a considerable variety in the presence of it's short arm.
혈액세포를 이용한 염색체 분리 분석에 관한 방법적 고찰
손시환,정구민,Sohn, S.H.,Chung, K.M. 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.2
This study was carried out to develop the methodology of chromosome preparation from blood cultures in mammals which included human, mouse, cattle and pig. For karyotyping, 0.5-5.0ml of peripheral blood were collected and cultured. The satisfactory results were obtained from macroculture and microculture in all species. In culture, the patterns of cell growth were no difference among media except serum concentration and mitogen supplement. The presence of mitogen and fetal bovine serum in medium significantly affected the mitotic index. The optimal culture condition was 37$^{\circ}C$ for 3 days. And the concentration of colcemid and reincubation time also affected the chromosome morphology. In harvest, chromosome patterns were mainly affected on hypotonic treatment which included treated time and temperature, dropwise of fixative solution, and drying after slide preparation.