Shigella flexneri ATCC 29903를 이용한 ERIC-과 REP-PCR 반응 구성성분 농도의 최적화

서현아,김근성 중앙대학교 유전공학연구소 2001 遺傳工學硏究論集 Vol.13 No.1

We attempted to optimize the concentrations of PCR components such as MgCl2, Primers, dNTPs and template DNA to produce fingerpriting patterns of the ERIC- and REP-PCR products for Shigella flexneri ATCC 29903, The ERIC-PCR reactions resulted in a total number of 6 to 9 fragments and the REP-PCR reactions a total number of 10 to 14 fragments. These Phenomena can be explained by the hypothesis that more copies of REP sequences are present on bacterial chromosomes than those of ERIC sequences are. ERIC-PCR reactions generated two major bands of 0.7kb and 1.2kb DNA fragments. They also had the number of PCR products and their band intensity increased as the concentrations of the above four PCR components were increased REP-PCR reactions generated multiple PCR Products between 0.6kb and 4.1kb in size including three major bands of 0.6kb, 0.7kb and 2.2kb DNA fragments. Their band intensity was also increased as the concentrations of the above four PCR components were increased. The PCR reaction cocktails containing 2.0mM MgCl2, 2.0μM primers, 200μM dNTPs and 1.0㎕ template DNA, respectively, yielded the maximum number of PCR products. As the above four components were added more than the concentrations mentioned above in PCR reaction cocktails, the number of PCR products was not increased and the overall fingerprinting patterns remained unchanged. Therefore, a PCR reaction cocktail containing 2.0mM MgCl2, 2.0μM primers, 200μM dNTPs and 1.0㎕ template DNA can be suggested to generate the most optimized PCR fingerprinting patterns .

豚舍上部空間을 利用한 家兎飼育이 豚의 增體에 미치는 影響 : 第Ⅲ報 家兎糞尿 및 殘飼의 自由採食이 飼料給與水準을 달리했을 때 離乳仔豚의 成長에 미치는 影響 Ⅲ. The effect of liberal feeding of rabbit feces, urime and residual forage on the growth under the resrticted feeding of weaned pigs

徐大振,鄭鉉丞 진주산업대학교 1979 論文集 Vol.17 No.-

This experiment was carried out to determine the effect of liberal feeding of rabbit feces and residual forage on the growth of weaned pigs subject to restricted feeding in which the daily allowance of formula feed was 3% of body weihgt, compared with 4% in the controls. The rabbit feces and residual forage were to be dropped from the hutches over the pig barns. Ten pigs of the same litter were weaned at about 20㎏ of body weight and alloted in egual numbers into the control and experimental group. The growth results from feeding trial for 2 months are summarized as follows: 1. The body weight gain for 2 Months was averaged 27.0㎏ and 30.6㎏ in the control and experimental pigs, respectively, However, there was found no significant (p>0.05) difference in the body gain between both the groups. 2. The feed conversion was improved slightly in the experimental group(2.9) as compared with the control(3.3) 3. The rabbit waste and residual forage ware found considerably palatable to the pigs. 4. The liberal feeding of rabbit feces and residual forage resulted in the decrease of about 40 won in the feed cost for 1㎏ body weight gain of pigs. 5. The pigs fed rabbit waste and residual forage were found to be normal clinically.

대구경 볼트와 와이어텐션을 이용한 슬래브 보강공법에 관한 실험적 연구

서동기,노현섭,김상모,이창남,김상섭 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

As time goes up, structural capacity of members decreases in reinforced concrete structures. The deterioration of the capacity is mainly due to the poor construction, corrosion of steel bars and the neutralization of concrete due to environmental pollution, etc. This deterioration is unavoidable, thus it is necessary to develop the method for repair and retrofit of structures in order to ensure structural safety of members. The purpose of this paper is to investigate the load-deflection, ultimate strength, failure mode, and energy absorption capacity of reinforced concrete slabs subject to load experimentally for several variables and grasp the problem of concrete slabs strengthened with large diameter bolts and wire tensioning. And propose foundational research data for4 strengthening reinforced concrete slabs. The test result shows that the capacity of anchorage is controlled by bearing capacity of concrete under the anchorage or punching shear around the anchorage. And the gap between bolts and core hole must be filled to prevent slip and rotation of anchorage.

Staphylococcus aureus KN556를 이용한 ERIC-과 REP-PCR 반응 구성성분 농도의 최적화

서현아,김영준,김근성 中央大學校 遺傳工學硏究所 2002 遺傳工學硏究論集 Vol.15 No.1

본 실험은 Staphylococcus aureus KN556의 ERIC과 REP-PCR 산물의 fingerprinting pattern의 파악과 PCR 구성성분의 농도의 최적화를 목적으로 실시하였다. 실험결과 ERIC-PCR은 증폭된 단편수가 4∼10개 정도로 나타냈고, REP-PCR은 전체 단편수가 5∼14개를 나타내었다. 이러한 현상은 ERIC sequence에 비해 REP sequence가 세균의 염색체 상에 빈도가 더 높게 분포한다는 이론과 일치하였다. ERIC-PCR의 경우, 0.8과 1.2kb를 중심으로 작은 분자량과 큰 분자량의 범위로 점차적으로 확대되어 각 변수들의 농도의 증가에 따라 전체단편의 수와 단편들의 강도가 증가하였다. REP-PCR은 최소분자량 0.25kb에서 최대분자량 3.5kb 정도 크기의 증폭된 단편들이 각 농도에 따라 강도의 차이를 나타내며 증가되는 것이 관찰되었다. 각 성분변수별로 MgCl_2는 2.0mM, primer는 2.0μM, dNTPs는 200μM, DNA는 1.0㎕에서 각각 최대 단편수를 나타냈고, 그 이상의 농도 적용 시에도 동일한 단편의 수를 유지하였으며 각 성분농도 변수 PVR 결과 나타나는 DNA fingerprinting pattern이 동일하게 나타나서 결과적으로 PCR 성분별 최적농도는 MgCl_2는 2.0mM, primer는 2.0μM, dNTPs는 200μM, DNA는 1.0㎕로 결론 지을 수 있었다. We attempted to optimize the concentrations of PCR components such as MgCl_2, primers, dNTPs and template DNA to produce fingerprinting patterns of the ERIC- and REP-PCR products for Staphylococcus aureus KN556. The ERIC-PCR reactions resulted in a total number of 4 to 10 DNA fragments and the REP-PCR reactions a total number of 5 to 14 DNA fragments. These phenomena can be explained by the hypothesis that more copies of REP sequences are present on bacterial chromosomes than those of ERIC sequences are. ERIC-PCR reactions generated two major bands of 0.8kb and 1.2kb DNA fragments. They also had the number of PCR products and their band intensity increased as the concentrations of the above four PCR components were increased. REP-PCR reactions generated multiple PCR products between 0.25kb and 3.5kb in size including three major bands of 0.7kb and 0.95kb DNA fragments. Their band intensity was also increased as the concentrations of the above four PCR components were increased. The PCR reaction cocktails containing 2.0mM MgCl_2, 2.0μM primers, 200μM dNTPs and 1.0㎕ template DNA, respectively, yielded the maximum number of PCR products. As the above four components were added more than the concentrations mentioned above in PCR reaction cocktails, the number of PCR products was not increased and the overall fingerprinting patterns remained unchanged. Therefore, a PCR reaction cocktail containing 2.0mM MgCl_2, 2.0μM primers, 200μM dNTPs and 1.0㎕ template DNA can be suggested to generate the most optimized PCR fingerprinting patterns.

유전치의 polyethylene fiber-post를 이용한 심미수복에 관한 증례 보고

서영주,조태식,김효석,고승백,정현구,이난영 조선대학교 구강생물학연구소 2002 口腔生物學硏究 Vol.26 No.2

In clinical practice, restoration of primary incisors severely affected by caries continues to be a challenge. Not only are these teeth difficult to restore. but the behavior of the patient can affect treatment negatively. An acceptable restoration for maxillary anterior incisors should have matching material color, durability, adhesive cementation that is biocompatible with the pulp, easily and rapidly placed. This Paper describes two cases showing an alternative technique for the construction of completely destroyed upper incisors with polyethylene ribbons for retention and stability for the resin crowns.

만성경부통증 환자에 대한 근막이완술과 경피신경 전기자극 치료가 치료기간에 따라 관절가동범위와 통증에 미치는 영향

서현규,공원태,이상용 대한정형도수치료학회 2005 대한정형도수물리치료학회지 Vol.11 No.2

The purpose of this study is compare the effects of two therapeutic processes-ⅰ) conservative Myofacial Release Technique along and ⅱ) Transcutaneous Electrical Nerve stimuliation alone - on the increase in the range of motion(ROM) and on the decrease in patient's pain with chronic Neck pain. The subjects of the study were student with choronic neck pain in Daegu health university. Half of them (10) took conservative Myofacial Release Technique along and the others (10) took Transcutaneous Electrical Nerve stimuliation alone.I used 3D Motion analysis. The range of motion (ROM) in six areas-flexion, extension, left lateral flexion, right lateral flexion, left rotation, right rotation-to check the effectiveness of the two combinations of methods, measured pain with visual analogue scale (VAS) to see the effect on pain reduce. Assement was conducted to the groups before the treatment began and affer the four weeks treatment ended. This study shows that both groups demonstrated significant improvement in ROM increase and pain reduction. Flexion ROM increases were significant in MFR and TENS after treatment 2 weeks and 4 weeks. Extension ROM increases were significant in MFR and TENS after treatment 2 weeks. Right rotation ROM was no significant differences were found in MFR and TENS after treatment all weeks. Right lateral flexion ROM increases were significant in MFR and TENS after treatment 3 weeks. Left lateral flexion ROM increases were significant in MFR and TENS after treatment 1 weeks. But the others no significant differences were in MFR group and TENS group during 4 weeks.

2004학년도 신입생 실태조사

서정조,김두화,김성현 濟州大學校 學生生活硏究所 2005 學生生活硏究 Vol.26 No.-

The purposes of this survey are to collect and analyze basic data on freshmen, to understand students' general matters and to utilize the data as basic materials for student guidance and administration; to find out the students' general matters, we asked freshmen about their psychological character, values, university life, impression and pride on university, the reason for choosing their majors, whether they will keep studying, the relations with professors, life in their high school, social relationship, the point of view on social issues, and to what extent they turn to the consultation service center. The survey was conducted among 2,832 freshmen during the orientation course in 2004, and 1,812 students (64% of the total) answered. The results are as follows; 1. Most freshmen live in Jeju. The number of students from other regions and cities is also increasing gradually, so the university should create conditions to accommodate those who want to use the dormitory 2. The increase in students who have entered the university after taking entrance examination more twice indicates that they want to pursue a study in line with their aptitude. Most students' supporters are their fathers. 84.8% of the respondents have both a father and a mother. which shows that broken families became a serious social issue. The educational background of parents is getting higher. They are mostly engaged in agriculture, fisheries, and forestry. Many students' family income is over 1.5 million won a month, but a lot of students do not know about their financial status, showing their indifference in home economy. 3. Most of the freshmen replied that they live happily with their family. As to the relationships with their parents, most were positive, but some students feel uncomfortable with their parents or even say their parents do not care about them. 4. About their mental and emotional status, 73.4% showed a positive response. Their tuition fees and living expenses are primarily covered by their parents, but the number of such students has decreased compared to last year. 5. Their motivation of university entrance is to get well-paid jobs, develop their abilitiesand acquire professional knowledge. They want to make many friends and study hard. 79.5% of respondents said they want to study or travel abroad. Accordingly, the authorities need to offer as many chances as Possible. 6. Most students do not want to live in the dormitory, and many (43.9%) want scholarship and part-time jobs to pay tuition fees and other expenses so that more funds and programs for scholarship should be secured. Most of the freshmen are satisfied with the CNU website. They want to study a major that can be related to the career to pursue. 7. Some respondents said they will join clubs for their hobby or sports because they want to make many friends and do much experience. Others said they do not want to join any club because they do not feel any need. Programs to encourage students to participate in a club: should be developed. 8. They think; what is important in their life is leading a financially stable life and the exertion of their ability, and the attitude they have as a college student is to prepare themselves to become an able member of society and intellectuals. 9. The freshmen had a good impression on Cheju National University (CNU) before entering the university, but most of them do not know well about CNU, which means public relations were not enough: What they took into account when applying was the possibility of success given their scores. Their aptitude and qualifications were second. They got information on the university mostly from the website of CNU. This reminds us of the importance of on-line homepages. On the other hand, the university exposition tour arranged by CNU turned out to be of little help to applicants. 10. The publicity commercial conducted every November- December does not attract attention from students. Among means of public relations targeting students living outside Jeju, nationwide, mass media or in-person visit to high school bear good result. Publicity activities need not only efforts of officials in charge but also attention of all staff, faculty and students of the university. 11. Most of the freshmen are proud of entering CNU. They decide their major whey in their third year of high school or during the applying period. The number of students who decided their major when they were in middle school is on the decrease, which indicates that students select their major without discretion instead of setting a goal in advance and trying to achieve it. 12. Freshmen are satisfied with their major. However, they neither know well about a transfer system nor a double major system. As to double major, the most preferred second major is education. The reason for pursuing double major is to have more chance of employment, which shows students understand the difficulty in getting a job from the beginning of entrance. 13. The students expect advice on fields of study as well as close relations from them. The students have relatively integrated relations with others. They prefer lecturer-centered or experiment classes. When faced with troubles, they talk to their friends or mother, in some cases to their siblings and father. The social relations are largely good, but 4.1% confessed to their problem in relations with others. Programs for relationship development and improvement are needed. 14. Freshmen consider efforts and capability as the requirements for social success. They picked unemployment as the most serious problem faced with Korean society. They consider poor local development and backward cultural facilities as challenges faced by Jeju. Therefore, social and political leaders are urged to realize the situation and deal with regional development of Jeju with caution. 15. The students want to get advice from the consultation service center on personal problems. What they want to talk about are jobs, scholastic performance and graduate schools. They want to take aptitude tests. They are also thinking about volunteering to help others. Many students want to build their social experience through volunteer public service, so various service programs should be develop.

식중독 세균 5속을 검출하기 위한 4개 주요 REP-PCR 반응성분의 최적화

서현아,박성희,송대식,김근성 한국산업식품공학회 2003 산업 식품공학 Vol.7 No.4

반복성 염기서열을 응용한 PCR법인 REP-PCR을 사용하여 식중독 미생물의 분리와 동정을 위한 연구에 적용하기 위하여 주요 식중독 유발세균 5속(genus), 6종의 균주를 사용하여 실험을 실시하였다. PCR 구성성분인 MgCl₂, dNTPs, REP sequence primer, 주형 DNA의 농도를 최적화하기 위한 연구 결과, MgCl₂의 농도가 2.0mN과 2.5mN일 때, 균주들의 fingerprinting pattern의 변화가 없거나 적은 것으로 관찰되어, 모든 속의 균주를 단일조건으로 분리?동정하기 위해서 MgCl₂의 농도는 2.5mN이 최적인 것으로 결론을 내렸다. dNTPs는 50μM의 농도를 적용하였을 때부터 전체 fingertprinting pattern범위의 주 단편들을 나타내는 균주들도 있었으나 총 단편의 수가 완벽하게 나타나지는 않았고, 200μM의 적용시점에서 6종의 균주 모두 단편의 수나 강도의 변화가 관찰되지 않았기 때문에 fingerprinting pattern의 파악을 위한 목적에는 200μM의 dNTPs 농도만으로도 충분하였다. REP primer는 적용한 농도가 증가함에 따라 단편의 수와 강도가 증가하였으며 Vibrio를 제외한 5종의 균주가 2.0μM의 primer를 적용했을 때 고유한 fingerprinting pattern을 나타냈다. 주형 DNA의 양을 변화시켜 적용하였을 때 DNA 양의 비율이 2배에서 5배까지 증가하여도 초기 미량 적용 시 생성되었던 fingerprinting pattern은 많은 차이를 보이지는 않았다. 그러나 적용되는 DNA 양에 따라 단편들의 강도와 수가 약간씩 점증하는 양상을 보였다. This experiment was conducted to optimize REP(repetitve extragenic palindromic sequence)-PCR reaction conditions in order to simultaneously differentiate the five different foodborne pathogenic bacterial genera of Escherichia, Salmonella, Shigella, Vibrio and Listeria. The four major PCR cocktail components of MgCl₂, dNTPs, primers and teplate DNA were considered and their optimum concentrations were determined using six reference strains. the optimized condentration of MgCl₂ was determined to be 2.5 mM in order to obtatin a consistent fingerprinting pattern. The similar fingerprinting pattern was obtatined when REP primers and dNTPs were added up to the concentration of 2μM and 200μM, respectively. As for template DNA, the numbers of PCR fragments were not mostly affected, but their intensities were slightly increased as the concentrations of the DNA were increased from 2 times to 5 times.

고층사무소건물의 매스구성을 위한 전문가 시스템의 개발에 관한 연구

서정훈,박상현,권종욱 영남대학교 환경문제연구소 2000 環境硏究 Vol.20 No.1

건축에서의 전문가 시스템은 설계자에게 많은 가상적인 해결안을 반복해서 수집해야할 수고를 줄여주고 모델링이나 정보제공과 같은 정형적인 측면과 설계의 판단, 상황별 특성과 같은 비정형적인 측면을 연결시키는 도구로 작용한다. 본 연구는 고층사무소건물의 설계과정 중 매스 구성 단계에서 중요한 역할을 하는 요소를 찾아내고, 그 요소들의 정형화 및 표준화 작업을 통해 고층사무소건물의 매스구성을 보조하는 전문가 시스템을 개발하는데 그 목적을 두었다. 본 연구에서 얻어진 결론을 요약해보면 다음과 같다. 건축설계작업 중 매스구성 단계를 제한조건을 만족시켜나가는 제한조건 만족모델로 인식할 수 있었으며, 이 제한조건의 일부를 체계화하여 건물의 매스구성을 보조할 수 있는 시스템을 개발하는 것이 가능하였다. 또, 개발된 시스템을 실제 사례를 통해 평가해 본 결과 타당성 있는 결과물을 산출할 수 있었다. In architecture, the expert system helps architects reduce unnecessarily repeated process. It also serves as a tool of linking patterned processes such as medeling and information provision, with unpatterned processes like decision-making process which can produce different results depending on the situation. This study is devoted to develop an expert system which is helpful to find out the important factors in massing process and to assist the massing of high-rise office buildings through patternization and standardization. The conclusion from the study as follows; It is definitely possible to make the expert system recognize the massing process as a process of meeting all the constraints on a step-by-step basis. So, the developed expert system can assist the massing process by systemizing parts of the constraints. It also proved that the developed knowledge-based system can produce proper outputs as its test showed during the study.

남아공 민주화와 노동조합의 역할 : COSATU를 중심으로

서상현 韓國아프리카學會 韓國外國語大學校 아프리카연구소 공동 2001 한국아프리카학회지 Vol.14 No.-