일개 대학병원에서 경험한 방선균증의 임상상

정숙인,김연숙,기현균,김춘관,김신우,이혁,김성민,백경란,송재훈 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.3

배경 : 방선균증은 혐기성의 그람양성 간균에 의한 만성 화농성 감염으로, 경안면부, 흉부, 복부, 골반부 등을 주로 침범한다. 최근 구강 위생의 향상과 항생제의 사용으로 인해 방선균증의 빈도의 감소와 함께 그 임상양상 또한 크게 변화하였다. 저자들은 국내에서의 방선균증의 임상상을 분석하기 위해 본 연구를 시행하였다. 방법 : 1995년 5월부터 1998년 9월까지 삼성서울병원에서 방선균증으로 진단받은 환자를 대상으로 임상기록지를 토대로 감염병소와 임상경과 등을 후향적으로 조사하였다. 결과 : 방선균증 환자는 모두 12명으로 경안면부 1례, 흉부 3례, 복부 3례, 골반부 5례였다. 임상양상은 주로 종괴의 형태로 나타나 진단을 내리기까지 종양과의 감별에 어려웠으며 진단은 모두 조직검사나 흡인액에서 유황과립을 확인함으로써 이루어졌다. 방선균증으로 진단된 환자는 모두 호전소견을 보였다. 결론 : 국내에서도 방선균증이 드물지 않게 발생하고 있으며 임상양상이 다양하여 가장 많이 오진되는 질환 중의 하나이다. 방선균증은 조직검사나 균배양검사를 통해 진단하고 항생제를 통해 완치될 수 있는 질환이므로 환자 진단에 관심을 가지면 불필요한 수술을 피할 수 있을 것으로 생각된다. Background: Actinomycosis is an indolent, slowly progressive bacterial infection, caused by Actinomyces. a gram-positive, non-spore-forming anaerobic or microaerophilic rod. Actinomycosis affects nearly every organ and body site. The disease is classically divided into four types, depending on the anatomic sites involved: cervicofacial, thoracic, abdominal, and pelvic. It has been considered as a rare disease, and only several cases have been reported in Korea. We performed this study to analyse the clinical manifestations of actinomycosis in Korea. Method: We retrospectively reviewed the medical records of patients with actinomycosis at the Samsung Medical Center from May 1995 to December 1998. Results: During the study period, actinomycosis was diagnosed in 12 patients: 1 patient had cervicofacial lesion, 3 thoracic, 3 abdominal, and 5 pelvic. The male and female ratio was 1 : 2 and the mean age was 47.2 years. Sulfur granule was observed in the pathologic specimens from all cases, but organism did not grow at all. It was very difficult to differentiate actinomycosis from tumorous conditions without pathologic diagnosis. All patients were clinically improved with antibiotics or surgical procedures. Conclusion: Actinomycosis is not a rare disease nowadays and has various clinical manifestations. It can be diagnosed by pathologic examination or culture and cured by antibiotics without surgical management.

실시간 in-situ IR을 이용한 IPDI와 Dimethylol Alkanoic Acid계 폴리우레탄 프로쫄리머의 반응속도론 연구

김정균,강신춘 한양대학교 이학기술연구소 2003 이학기술연구지 Vol.6 No.-

폴리우레탄 생성을 위한 프리폴리머 중합의 속도론적 연구를 위하여 정량적 분석이실시간으로 가능한 in-situ IR을 사용하고 IPDI(isophoron diisocyanate)와 DMPA(dimethylol propionic acid)의 용액중합(IP 반응)과 IPDI와 DMBA(dimethylol butylic acid)의 용액중합(IB 반응)을 반응온도 60-90℃ 및[NCO]/[OH]=1.0하에서 수행하였다. 반응 속도는 일련의 IR 스펙트럼에서 NCO 신축진동흡수(2265cm^(-1) 및 C=O 신축진동흡수(1725 cm^(-1)대의 흡광도 변화를 모니터링하여 얻었다. 반응은 수산기와 이소시아네이트기의 농도에 대하여 각각 1차로 표시할 수 있었으며, 전체적으로는 2차 반응속도 법칙에 따랐다. 반응 속도 상수로부터 구한활성화 파라미터는 IP 반응의 경우 E_(a)=42.2 K·J/mol, ΔH=39.3kJ/mol 및 ΔS=-146.7 J/mol·K이며, IB 반응은 E_(a)=36.9 K·J/mol, ΔH=34.0kJ/mol 및 ΔS=-163.3 J/mol·K Kinetics of pre-polymerization of polyurethane formation between IPDI(isophoron djjsocyanate) and DMPA (dimethylol propionic acid) in solution state(IP), and IPDI and DMBA(dimethylol butanoic acid) in solution state(IB) were studied by using quantitative real-time in-situ IR spectroscopy at different temperatures in the range of 60-90 °c with [NCO]/[OH] ratio of 1.0. The reaction rate was obtained from monitoring the change of NCO (2265 cm^(-1)) and C=O (1725 cm^(-I)) stretching band in series IR spectra. The reaction was in accord with the first-order for the concentration of hydroxyl groups and isocyanate groups respectively, which conformed to the second order law entirely. The activation parameters obtained from the evaluation of kinetic data are E_(a)=42.2 kJ/mol, ΔH =39.3 kJ/mol and ΔS =-146.7 J/mol'K for IP and E_(a)=36.9 kJ/ mol, ΔH = 34.0 kJ/mol and ΔS = -163.3 J/mol'K for lB

菊花의 揷木方法改善에 對하여

嚴聖均,朴重春 진주농과대학 1968 진주농과대학 연구논문집 Vol.- No.7

從來의 繁雜한 菊花揷木法의 改良을 目的으로 揷床에 水分保有量을 달리하는 5區를 만들어 比較試驗하였다. 1. 對照區인 볕가림 없는 보통式 揷床에서는 揷穗가 2∼3日後 全部枯損되었으나 그 外의 造作한 試驗區에 있어서는 枯損의 率이 적었다. 2. 本 實驗의 5個의 造作區에서는 個體當 草長, 發根數, 總發根長을 調査하여 그 試驗區別, 成績을 各各 分散分析한 結果 1%의 高度의 有意性이 認定되었으므로 水分保有量이 菊花揷木에 있어서 顯著한 影響을 미침을 알수 있었다. 3. 各試驗區의 平均間 有意差를 檢定하기 爲하여 Kramer의 多重檢定을 한 結果 草長, 發根數, 總發根長 모두 水分保有量이 많은 A區에서 작은 E區의 順으로 良好하며 有意差는 草長에서 A區와 E區間에 發根數에서 A區와 C,D,E區間에 總發根長에서는 A區와 D,E區間에 1%의 水準의 高度의 有意性이 認定되었다. 以上의 諸結果로 보아서 適當한 水分保有量을 確保할 수 있는 揷床을 造作함으로서 까다로운 菊花揷木法을 若干解消할 수 있는 可能性을 暗示하였다. The comparative test was performed on five cutting pots whose moisture content was respectively different, with a view to improving the conventional complicated cutting methods to nurse chrysanthemum. Five different cutting pots were made from A to E. Drainage tube was made 9 cm height from bottom in pot A, and the rest were also made 7 cm, 5 cm, 3 cm and 0 cm height from bottom in pot B,C,D and E, respectively. The results obtained are summarized as follows: 1. The control plots, when unscreened from sunlight, withered without exception in less than two or three days after implanted, but on the treated plots they promised to survive even under the unscreened condition. 11. Plant height, number of rooting-cutting, total length of rooting-cutting were examined from each of the five treated plots on the basis of individual plants. From the results by the analyses of variances as shown in table 1,3 and 5, it was recognized that highly significant differences among the treatments were observed for all characters. This enabled the author to realize that moisture content had on outstanding influence on chrysanthemum cutting nursing. III. The recorded results had been analyzed statistically by the method of Kramer's multiple range test. According to the Kramer's multiple range test in all experiments, there was not significant difference at the 1% level(p<0.01)for plant height between A plot and the other plots. On the other hand, there was not significant difference at the 5% level(p<0.05)for plant height between C plot and the other B, D plots and between D plot and E plot. For number of rooting-cutting, there was not significant difference at the 1% level between A plot and B plot and between B plot and the other C,D,E plots. And for total length of rooting-cutting, there was not significant difference at the 1% level between A plot and the other B, C plot and between B plot and the other C,D,E plots. The results were proven to be excellent in plant height, number of rooting-cutting and total lenght of rooting-cutting, told in order, from plants from the cutting pots of highly hydrous plots to those of the cutting pots of plots containing less water. The above results suggested the possibility that the conventional complicated methods of cutting chrysanthemum can be improved to some extent by using well-prepared cutting pots capable of securing a proper amount moisture.

조직학적 진단된 chronic necrotizing pulmonary aspergillosis 2예

김연숙,정숙인,기현균,김춘관,김신우,한정호,김성민,백경란,송재훈 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.1

CNPA는 기존의 폐질환 및 폐절제술로 인해 국소방어기전에 저하가 있거나 비특이적 전신면역상태저하를 갖고 있는 환자들에게서 주로 발생하는 만성적인 공동성 폐질환이다. 현재까지 우리나라에서는 CNPA의 보고가 없는 상태이고, 저자들은 최근 조직학적으로 진단된 CNPA 2예를 경험하였기에 보고하는 바이다. 저자들이 경험한 CNPA 환자들은 만성알코올 중독 및 기관지확장증과 같은 기저질환과 폐절제술을 받은 병력이 있었다. Aspergillus가 폐실질을 침윤하고 있는 조직소견과 조직배양에서 Aspergillus가 검출되어 조직학적 진단이 가능하였고, amphotericin B의 정주요법 및 경구 itraconazole 투여와 폐절제술 등으로 성공적으로 치료되었다. 아직 많은 임상의들에게는 낯선 질환인 CNPA의 치료성적은 환자의 동반질환 및 CNPA 자체의 중증도, 진단과 치료 시작의 지연 등에 의해 크게 좌우되므로 만성적으로 진행하는 공동성 폐병변을 갖고 있는 환자에게서 CNPA를 감별하는 것이 중요하다. Chronic necrotizing pulmonary aspergillosis (CNPA) is a chronic cavitary form of pulmonary aspergillosis. Dozens of CNPA cases have been reported in patients with systemic immunologic dysfunction or altered local defense mechanism from preexisting pulmonary disease. Review of literatures revealed that no CNPA cases have been reported in Korea yet. We experienced two cases of CNPA proven by lung biopsy. A 53-year-old alcoholic male in poor nutritional state was admitted with generalized weakness and weight loss. Chest CT revealed a cavitary nodule surrounded with ground-glass attenuation. CT-guided fine needle aspiration and biopsy was done. The biopsy specimen demonstrated dichotomously branching septated hyphae consistent with those of Aspergillus sp. Another case was a 39-year-old man with bronchiectasis who was admitted with persistent hemoptysis. He had a past history of pulmonary tuberculosis. A parahilar lesion with intracavitary soft tissue mass was incidentally detected in high-resolution GT. Left lingular segmentectomy was done due to uncontrolled hemoptysis and CNPA was histologically diagnosed. Both patients were successfully treated with intravenous amphotericin B followed by oral itraconazole. Even though CNPA is unfamiliar to most clinicians, it should be included in differential diagnoses of chronic progressive cavitary pulmonary lesion, especially in patients with immunologic dysfunction.

토끼 수막염 모델을 이용한 다제내성 폐렴구균에 대한 Meropenem의 치료 효과

김신우,진정화,강수정,정숙인,김연숙,기현균,김춘관,이혁,김성민,백경란,송재훈 대한화학요법학회 2001 대한화학요법학회지 Vol.19 No.1

목적 : 다제내성 폐렴구균에 의한 수막염은 기존과 단일 항생제로는 근치가 어려워 ceftriaxone과 vancomycin 혹은 rifampin의 병용요법이 권유되고 있다. 다제내성 폐렴구균에 사용할 수 있는 다른 약제 중 meropenem은 시험관내 폐렴구균에 대한 항균력이 좋고 경련 등의 중추신경계 부작용이 거의 없어 다제내성 폐렴구균에 의한 수막염의 치료에 이용할 수 있을 것으로 기대되나 이에 대한 자료가 매우 부족한 상태이다. 이에 연자 등은 meropenem과 meropenem+vancomycin의 병용요법이 다제내성 폐렴구균 수막염의 치료에 효과가 있는지를 토끼 수막염 동물 모델을 이용하여 검증하고자 하였다. 방법 : 인체에서 수막염을 일으킨 다제내성 폐렴구균 균주(penicillin MIC 2, ceftriaxone MIC 4, meropenem MIC 0.5㎍/㎖)를 토끼 척수강 내에 직접 주입하여 수막염을 유발한 후 ceftriaxone, vancomycin, rifampin, meropenem의 단일 항균요법, ceftriaxone+vancomycin, ceftriaxone+rifampin, meropenem+vancomycin의 병용요법 간에 치료 효과의 차이를 비교 하였다. 각 치료군은 6 마리의 토끼를 대상으로 하였다. 각각의 항생제는 5 시간 간격으로 2번 정주하였으며, ceftriaxone은 1회 투여하였다. 균주 주입 후 각 치료군이 토끼에서 치료 후 0, 5, 10, 24 시간에 척수액을 채취하여 균주의 수를 측정하여 24시간째의 살균 여부를 최종 치료 효과로 판정하였다. 결과 : meropenem 단독 요법은 10시간째에 모든 균주를 살균하였으나 24시간 째에 다시 균의 성장이 관찰되었으며, meropenem+vancomycin의 병용요법은 단독 약제보다 우수한 살균효과를 보였으나 상승작용은 없었다. 결론 : meropenem+vancomycin의 병용요법은 기존의 ceftriaxone+vancomycin보다 24시간째에 더 큰 균의 감소 경향을 보여(P=0.054) 임상에서 사용이 가능할 것으로 기대되며 향후 실제 수막염 환자에서의 임상효과를 증명하는 연구가 필요할 것으로 생각된다. Background : Because antimicrobial monotherapy for pneumococcal meningitis caused by penicillin-resistant strains is not satisfactory, ceftriaxone+vancomycin [C+V] or ceftriaxone+rifampin [C+R] regimens are recommended. Meropenern [M] is one of the monotherapy options for penicillin-resistant pneumococcal meningitis due to good in-vitro activity against pneumococci and rare adverse reactions in CNS. But there have been few reports in the efficacy of meropenern against pneumococcal meningitis. We evaluated therapeutic efficacy of the meropenern and meropenem+vancomycin in a rabbit model of meningitis caused by penicillin-resistant S. pneumoniae (PRSP). Methods : Meningitis was induced by intracistemal inoculation of a pneumococcal strain isolated from a patient with meningitis (MIC; penicillin 2, ceftriaxone 4, meropenem 0.5 g/㎖). Bacterial concentrations in the CSF were measured at 0, 5, 10, and 24 h after therapy was started. Therapeutic efficacy was evaluated by the final bacterial concentration in the CSF at 24 h. Results : C+V cleared the CSF at 10 h, but regrowth of bacteria was noted at 24 h. Meropenem monotherapy resulted in sterilization at 10 h but regrowth at 24 h. M+V was superior to M or V monotherapy but did not show synergism. Therapeutic efficacy of M+V was at least equal or superior to that of C+V (P = 0.054). Conclusion : Meropenem+vancomycin regimen could be one of the useful options in the treatment of PRSP meningitis. Clinical trials to evaluate the M or M +V are warranted in the future.

Biochemistry,Molecular Biology : Constitutive Expression of Defense Genes in Transgenic Arabidopsis Overproducing Methyl Jasmonate

Choon Kyun Jung,Seoung Hyun Lyou,Yeon Jong Koo,Jong Tae Song,Yang Do Choi,Jong Joo Cheong 한국응용생명화학회 2003 Journal of Applied Biological Chemistry (J. Appl. Vol.46 No.2

Transcript Profile of Transgenic Arabidopsis Constitutively Producing Methyl Jasmonate

( Choon Kyun Jung ),( Song Yion Yeu ),( Yeon Jong Koo ),( Min Kyun Kim ),( Yang Do Choi ),( Jong Joo Cheong ) 한국식물학회 2007 Journal of Plant Biology Vol.50 No.1

Non-Specific Phytohormonal Induction of AtMYB44 and Suppression of Jasmonate-Responsive Gene Activation in Arabidopsis thaliana

Jung, Choon-Kyun,Shim, Jae-Sung,Seo, Jun-Sung,Lee, Han-Yong,Kim, Chung-Ho,Choi, Yang-Do,Cheong, Jong-Joo Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.29 No.1

The Arabidopsis thaliana transcription factor gene AtMYB44 was induced within 10 min by treatment with methyl jasmonate (MeJA). Wound-induced expression of the gene was observed in local leaves, but not in distal leaves, illustrating jasmonate-independent induction at wound sites. AtMYB44 expression was not abolished in Arabidopsis mutants insensitive to jasmonate (coi1), ethylene (etr1), or abscisic acid (abi3-1) when treated with the corresponding hormones. Moreover, various growth hormones and sugars also induced rapid AtMYB44 transcript accumulation. Thus, AtMYB44 gene activation appears to not be induced by any specific hormone. MeJA-induced activation of jasmonate-responsive genes such as JR2, VSP, LOXII, and AOS was attenuated in transgenic Arabidopsis plants overexpressing the gene (35S:AtMYB44), but significantly enhanced in atmyb44 knockout mutants. The 35S:MYB44 and atmyb44 plants did not show defectiveness in MeJA-induced primary root growth inhibition, indicating that the differences in jasmonate-responsive gene expression observed was not due to alterations in the jasmonate signaling pathway. 35S:AtMYB44 seedlings exhibited slightly elevated chlorophyll levels and less jasmonate-induced anthocyanin accumulation, demonstrating suppression of jasmonate-mediated responses and enhancement of ABA-mediated responses. These observations support the hypothesis of mutual antagonistic actions between jasmonate- and abscisic acid-mediated signaling pathways.

A Case of Pulmonary Acrylic Cement Embolism Associated with Percutaneous Vertebroplasty

Hyun-Jim Jung · Gyeong-Yun Jung · Geum-Soo Lee · Dong-Ik Kim · Byeong-Kab Yoon · Gun-Woo Kim · Seung-Hyung Lee · Jong-Pil Jung · Young-Choon Ko · Won-Kyun Ra Yoon 조선대학교 의학연구원 2010 The Medical Journal of Chosun University Vol.35 No.2

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Chitin deacetylase from Absidia coerulea CHK-1 : Mycerial chitin deacetylase of a chitin deacelylase-hyperproducuing fungus, Absidia coerulea CHK-1

Park, Chun,Kim, Jung Ran,Shin, Jae Kyoung,Kim, June Ki,Lee, Tae Kyun,Chung, Ji Chun,Park, Weon Hwan,Park, Sun Dong,Nam, Kyung Soo,Lee, Young Choon,Kim, Cheorl Ho 한국키틴키토산학회 1997 한국키틴키토산학회지 Vol.2 No.2

A mycelial chitin deacetylase has been purified from a chitin deacetylase-hyperproducing fungus, Absidiα coerulea CHK-1. The purified enzyme had a molecular mass of about 62 kDa on denaturated and natural conditions. The pI was 5.5. The chitin deacetylase, when resolved by SDS-PAGE, was positive for Schiff staining, suggesting that the enzyme is a glycoprotein. When O-hydroxylated chitin (glycolchitin) was used as a substrate, the enzyme displayed a temperature optimum of around 50℃ and a pH optimum of around PH 5,5. The enzyme was stable to incubation from pH 3.0 to pH 6.5 at 4℃ for 24 hr. The presence of chitin protected the enzyme from heat inactivation, the extent depending upon the substrate concentration. The activity of the enzyme was stimulated by Mn2+ ion. The enzyme is active on chitooligosaccharides with more than two N-acetylglucosamine residues (M-acetylchitobiose). However, the enzyme is not active on N-acetylglucosamine. The enzyme had an apparent Km of 12.4 mM and Kcat of 32.4 /sec for glycol chitin, respectively.