식물생장조절물질의 정확한 사용을 위한 참고자료 -화훼류 재배를 중심으로-

엄성균 한국작물보호협회 1984 자연과 농업 Vol.5 No.7

여주(Momordica charantia L.)의 量的形質에 대한 遺傳分析 : 種子 및 子房關聯形質들의 遺傳分析

金周玹,嚴聖均 慶尙大學校 1989 論文集 Vol.28 No.2

여주의 果重을 目標로 早期에 選擇할 수 있는 效果的인 方法을 檢討키 위하여 果實特性面에서 相異한 4個育成系統을 材料로 이들을 二面交雜하여 兩親과 F1組合들을 pot에 심어 溫室內에 栽値하여 相觀및 遺傳分析을 試圖하였다. 相觀分析에서 調査된 形質은 子房長등 15個 形質이었고, 이중 種子 및 子房關聯形質인 種子길이, 種子幅,種子重,種子數,子房長,種子幅,種子重,果梗長, 8個形質에 대해서는 二面交雜分析을 하였다. 收量과 相關이 매우 높은 形質들은 果長, 果重, 子房長, 果梗長(花梗長), 種子幅등 이었으며, 이들 形質들은 相互間에도 높은 相關이 있었다. 種子數를 除外한 大部分의 形質들이 遺傳子의 優性 果보다 相加的效果가 컸으며, 特히 子房長과 果梗長, ,種子幅은 매우 높은 相加的遺傳子作用에 의해 支配되, 遺傳力도 매우 높았다. With a purpose to increase the effect of selection in bitter gourd(Momordica charantia L.), four inbred lines were crossed each other for a diallel cross set, and grown in the green house. The characters investigated were fruit length, fruit width, fruit weight, number of fruits, thickness of fruit flesh, days to maturity (from flowering) seed length, and yield. Correlation coefficient analysis revealed that six characters, i.e., fruit weight, fruit length, ovary length, peduncle length, seed width, and yield showed highly positive correaltions each other. Among 15 characters, eight characters, such as ovary length, ovary width, ovary weight, seed length, seed width, seed weight, number of seeds, and peduncle length, showed partial dominance in Vr-Wr graphical analysis, but they showed different degree of dominance. Overy length, seed length, seed width, and seed weight were controlled by additive rather than dominant effect of genes, and they had very high heritabilities in both broad and narrow sense, while in ovary weight, ovary width, and peduncle length, additive effect of genes was similar to dominant effect. Number of seed per fruit was governed by dominant rather than additive effect of genes. Heritabilities(h²n) in the narrow sense were low or moderate for number of seed, ovary width, and ovary weight, and that of peduncle length was high.

남부지방 시설원예의 재배환경에 관한 연구 : 몇가지 시설내미기상(施設內微氣象) , 토양조건 및 작물의 무기양분(無機養分) 함량에 관하여 Characteristics of Microclimate and Soil Conditions , and their Influences on Nutritional Status of Crops

엄성균 경상대학교 경남문화연구소 1983 慶南文化硏究 Vol.6 No.1

Many off-season vegetables and flowers are grown in the greenhouse mostly in the southern part of Korea where climatic conditions are rather favorable. However, the information on crop growth conditions in the greenhouse has been limited. In this study, therefore, indoor environmental conditions such as light, temperature, and soil properties are reported. Nutrient absorption by house-grown vegetables and flowers was also studied. The areas covered in this study include Jinju(Jinyang included), Masan, Gimhae, Namgi, and Suncheon (Seungju and Gwangyang included). The results are summarized as follows: 1. When outdoor light intensity was high, variations in indoor light intensity was observed depending upon the type and orientation of plastic houses. The east-west oriented house had a better light reception in December, while the south-north oriented house was better in the spring. 2. Indoor light intensity increased from the ground surface to the plastic ceiling, but little difference was observed at the bottom half of the pause. The south side of the house always showed a higher indoor light intensity in the east-west oriented house. However, in the south-north oriented house, this relationship was dependent upon the time of the day: east and west side of the house had better light reception in the morning and afternoon hours respectively. The three-quarters type house showed a higher indoor light intensity than other types, The highest indoor light intensity was observed at noon, and there was little difference between morning and afternoon hours. Double layering of plastic films significantly reduced indoor light intensity when the outdoor light intensity was higher. 3. Indoor temperatures ranged from 4 to 11℃ at 9:00 a.m., from 11 to 15℃ at noon, and around 10℃ at 4:00 p.m. The semi-circular type houses showed higher temperature than others in the morning hours of the winter. On the other hand, the three-quarters type houses showed higher temperatures in the mornings of the spring and in the afternoons of all the time. 4. In February, the east-west oriented and the south-north oriented houses showed higher morning temperature, while the three-quarters type house showed higher afternoon temperature. All types showed the highest temperature at south side as will as the enter surface of the house. 1 he south-north oriented houses showed a higher minimum temperature differences between indoor and outdoor than the east-west oriented ones. The difference was higher in side-vertical type than in semi-circular type, and the three-quarters type being in-between. 5. Double layering of the house helped conserve indoor heat only on warm, sunny days. Its benefit was little on chilly or rainy days. Double layering helped keep a higher temperature during the daytime, but not at night. However, those with the tunnels in the double-layered house were able to keep higher temperatures during the daytime and at night as well. 6. The average soil pH of those vegetable-grown houses was 6.0 to 6.4, which is thought to be optimal for vegetable growing. The pH of flower-grown houses was 6.1 to 6.3 at Gimhae and 5.7 to 6.0 at Masan area. 7. Among the vegetable-grown houses, the highest humus content was observed at Suncheon area (about 6%), followed by Jinju (3.3%), Gimhae (2.5%), and Namgi (1.0%). For flower-grown houses, the humus content was about 6% at Masan and 2.5 to 3.5% at Gimhae area. 8. Soil nitrogen content of vegetable-grown houses was about at 30㎎ per 100g soil which can be classified as optimum. However, the nitrogen content at Suncheon area was twice from flowering to the final stage of the growth. Soil nitrogen content of those flower-grown houses ranged from 40 to 50㎎ per 100g soil. The NO₃^--N/NH₄^+-N ratios of vegetable- and flower-grown house soils were 5:5 to 6:4 and 5:5, respectively. 9. The available P₂O_6 was 180ppm at Jinju, 240ppm at Gimhae (vegetable-grown), 350ppm at Namgi, 370 ppm at Gimhae (flower-grown), 440ppm at Masan, and 470 ppm at Suncheon area. 10. The cation exchange capacity was about 10me/100g soil. The content of cations of vegetable-grown houses was 2 to 6 me of Ca^(++), 2.5me of Mg^(++), and 0.5 me of K^+ in 100g soil. In those flower-grown houses, the contents of Ca^(++), Mg^(++), and K^+ in 100g soil were 4.0me, 2.0me, and 0.7me, respectively. 11. Boron content of vegetable-grown soils was less than 0.8 ppm, and the deficiency would be expected especially at Gimhae (0.7ppm) and Jinju (0.4ppm) areas. The highest Mn^(++) was observed at Jinju area (55ppm), and less than 50 ppm at all other areas. Iron content was about 1.2% at all areas, except that at Suncheon area being the lowest at 0.49. 12. T he soil E.C. values of vegetable-grown houses ranged from 0.3 to 0.7 mmhos/㎝, while those of flower-grown houses ranged from 0.45 mmhos/cm at Masan to 0.8 mmhos/㎝ at Gimhae. 13. Cucumbers produced at Suncheon area contained the highest nitrogen content at 3.0%, while carnations at Gimhae area contained the least amount of nitrogen at 1.1%. Nitrogen deficiency would be expected in tomatoes and carnations at Gimhae area where the content was less than 2.0%. The nitrogen content generally decreased as plant growth advanced. 14. Phosphorus content in the leaves of the house-grown crops was about 0.5%. Analysis of house soils indicated that the phosphorus content of the soil could not be related to that of the leaves. 15. Potassium content was about 1.7% in the leaves of most crops, which was considered to be adequate for normal growth and development. However, potassium would become a limiting factor for producing high quality tomatoes which contained about 1.12%. 16. Calcium content was species-specific: 1.66% for prince melons, 0.9% for tomatoes, 0.59% for cucumbers, and 0.3% for carnations and chrysanthemums. To increase calcium absporption by crops, it was suggested that house soils not to be dried and potassium and NH₄^+-N application be reduced.

여주 ( Momordica charantia L. ) 의 양적형질의 유전분석 : 종자 및 자방관련형질들의 유전분석

엄성균,김주현 한국육종학회 1989 한국육종학회지 Vol.21 No.4

菊花의 揷木方法改善에 對하여

嚴聖均,朴重春 진주농과대학 1968 진주농과대학 연구논문집 Vol.- No.7

從來의 繁雜한 菊花揷木法의 改良을 目的으로 揷床에 水分保有量을 달리하는 5區를 만들어 比較試驗하였다. 1. 對照區인 볕가림 없는 보통式 揷床에서는 揷穗가 2∼3日後 全部枯損되었으나 그 外의 造作한 試驗區에 있어서는 枯損의 率이 적었다. 2. 本 實驗의 5個의 造作區에서는 個體當 草長, 發根數, 總發根長을 調査하여 그 試驗區別, 成績을 各各 分散分析한 結果 1%의 高度의 有意性이 認定되었으므로 水分保有量이 菊花揷木에 있어서 顯著한 影響을 미침을 알수 있었다. 3. 各試驗區의 平均間 有意差를 檢定하기 爲하여 Kramer의 多重檢定을 한 結果 草長, 發根數, 總發根長 모두 水分保有量이 많은 A區에서 작은 E區의 順으로 良好하며 有意差는 草長에서 A區와 E區間에 發根數에서 A區와 C,D,E區間에 總發根長에서는 A區와 D,E區間에 1%의 水準의 高度의 有意性이 認定되었다. 以上의 諸結果로 보아서 適當한 水分保有量을 確保할 수 있는 揷床을 造作함으로서 까다로운 菊花揷木法을 若干解消할 수 있는 可能性을 暗示하였다. The comparative test was performed on five cutting pots whose moisture content was respectively different, with a view to improving the conventional complicated cutting methods to nurse chrysanthemum. Five different cutting pots were made from A to E. Drainage tube was made 9 cm height from bottom in pot A, and the rest were also made 7 cm, 5 cm, 3 cm and 0 cm height from bottom in pot B,C,D and E, respectively. The results obtained are summarized as follows: 1. The control plots, when unscreened from sunlight, withered without exception in less than two or three days after implanted, but on the treated plots they promised to survive even under the unscreened condition. 11. Plant height, number of rooting-cutting, total length of rooting-cutting were examined from each of the five treated plots on the basis of individual plants. From the results by the analyses of variances as shown in table 1,3 and 5, it was recognized that highly significant differences among the treatments were observed for all characters. This enabled the author to realize that moisture content had on outstanding influence on chrysanthemum cutting nursing. III. The recorded results had been analyzed statistically by the method of Kramer's multiple range test. According to the Kramer's multiple range test in all experiments, there was not significant difference at the 1% level(p<0.01)for plant height between A plot and the other plots. On the other hand, there was not significant difference at the 5% level(p<0.05)for plant height between C plot and the other B, D plots and between D plot and E plot. For number of rooting-cutting, there was not significant difference at the 1% level between A plot and B plot and between B plot and the other C,D,E plots. And for total length of rooting-cutting, there was not significant difference at the 1% level between A plot and the other B, C plot and between B plot and the other C,D,E plots. The results were proven to be excellent in plant height, number of rooting-cutting and total lenght of rooting-cutting, told in order, from plants from the cutting pots of highly hydrous plots to those of the cutting pots of plots containing less water. The above results suggested the possibility that the conventional complicated methods of cutting chrysanthemum can be improved to some extent by using well-prepared cutting pots capable of securing a proper amount moisture.

배추와 무우의 屬間變異體 誘起에 關한 硏究 : 未成熱胚와 外1來 DNA와의 co-cultivation 및 屬間交雜胚의 培養 Co-cultivation of Immature Embryos with Exogenous DNA and Culture of Intergeneric Hybrid Embryos

嚴聖均,陳旬馥,朴贊熙,朴重春 慶尙大學校 1990 論文集 Vol.29 No.1

배추 및 무우의 未成熟?에 外來 DNA를 co-cultivation시킴으로서 變異體 誘起를 試圖하였으며 屬間交雜을 통하여 雜種體를 生産함으로서 遺傳的 多樣性을 넓히고 genetic barrier를 克服하고자 本實驗을 試圖하였다. 1.핵배추 및 궁중무우의 未成熟?와 相互 外來 DNA와의 co-cultivation ?培養에서 完全한 植物體의 獲得은 핵배추 ?와 궁중무우 DNA와의 co-cultivation 에서는 ?粉後 20日 以上 發育한 ?를, 궁중무우 ?와 핵배추 DNA와의 co-cultivation 에서는 ?粉後 25日 이상 發育한 ?를 置床했을때 效果的이었다. 이들의 分化率은 ?粉後 日數가 經過할수록 또한 外部 DNA 存在下에서 높았으며 특히 10㎍의 DNA 存在下에서 가장 效果的이었다. 器內에서 分化된 個體는 hypocotyl의 發育이 억제되거나 지나치게 伸張될 뿐만아니라 cotyledon이 swelling 되는 등의 非正常的인 個體가 대부분이었으며 이들 個體는 계속 發育되지 못했다. 外來 DNA 와 co-cultivation 시킨 後分化된 個體중 正常的으로 發育된 個體의 表現型과 및 ?白질 isozyme 分析 結果 control區와 差異가 없었지만 10㎍의 DNA濃度下에서 非正常的으로 發育된 個體는 ?白質 및 isozyme banding pattern 상에서 control 區와는 큰 差異를 보였다. 2.배추 및 궁중무우 間의 屬間交雜 ?의培養 궁중무우를 種子親으로 한 것보다 핵배추를 種子親으로 했을 경우 受精率이 높아 ?의獲得이 많았다.雜種?培養時 ?粉後 日數가 經過할수록 分化 個體數가 많았으며 分化된 個體의 葉型은 배추와 무우의 中間型을 , 花色은무우와같ㅇ느 白色을 나타냈다. 雜種體의 葉 ?白質과 isozyme 分析 結果 ?白質과 AcP의 banding pattern은 무우가 種子親일 경우에는 무우와 같은 banding pattern이 관찰되었지만 배추가 種子親일 때에는 배추와 같거나 배추와 무우의 混合形이 觀察되었다.MDH의 banding patterndms 4개의 雜種體中 배추와 같은 것이 1個體였고 나머지 3個體는 兩親에서 觀察되지 않은 새로운 banding pattern을 나타냈다. This experiment was conducted to produce genetic transformants through co-cultivation of immature embryos with foreign DNA.and to produce intergeneric hybrids between radish and Chinese cabbage. It was designed to help broaden the genetic diversity of crop plants and to overcome the genetic barrier. 1. Co-cultivation The later the embryos were removed from the mother plants.and the higher the concentration of exogenous DNA up to 10 ㎍/embryo.the better the differentiation of the explants. However, most of the differentiated plants were developed abnormally;leaving only a few plants with a mother-type morphology were obtained . Electrophoretic banding patterns of totalproteins and the isozymes of acid phosphatase of norma; plants obtained by co-cultivating Kungzung embryos with Haekbaechu DNA revealed that there was no significant difference in any of them . Only those plants developed abnormally exhibited different banding patterns of the isozymes and total ptoteins as well. 2. Intergneric hybridization With embryo culture techniques, intergeneric hybrids have been obtained between Chinese csabbage and radish. The fertilizxation rate was higher when Chinese cabage served as a female than radish served as female. The later the embryos were removed from the mother plants. the better the differentiation of explants. The embryos have developed into mature plants with the characteristics of the intermediate type of two plants kabnd resembled radish in flower color . Majority of the tetrad stage adn pollen grain of prent species are oraml. whereas those of intergeneric hybirds are abonormal. The banding patterns of total ptoteins revealed that a Chinese cabbage type or a mixed type of radish and Chinese cabbage was served as a female. The total proteins banding patterns of radish type wer observed only when radish served as a female. One Chinese cabbage type were MDH banding patterns were observed in the tybrids, 3 out of 4 hybrids exhibited new banding patterns which were not observed in the hybirds, 3 out of 4 hybirds exhibited new banding patterns which were not observed in the patterns. With the exception of minor band , the isozyme banding patterns of AcP were similar to the banding patterns of total proteins.

6. 여주 (Momordica charantia L.) 의 몇가지 果實形質의 遺伝에 関한 研究

嚴聖均(Um Sung-Kyun),朴重春(Park Jung-Choon),金周玹(Kim Zhoo-Hyeon) 한국원예학회 1984 한국원예학회 학술발표요지 Vol.2 No.1

南部地方 施設園藝地帶의 하우스內 土壤의 化學的 性質에 關한 硏究

嚴聖均(Um Sung-Kyun),姜淮(Kang Hyae),河浩成(Ha Ho-Sung),朴重春(Park Jung-Choon) 한국원예학회 1979 Horticulture, Environment, and Biotechnology Vol.20 No.1

여주(momordica charantia L.)의 量的形質의 遺傳分析

Sung Kyun Um(嚴聖均),Zhoo Hyeon Kim(金周玹) 한국육종학회 1989 한국육종학회지 Vol.21 No.4

With a purpose to increase the effect of selection in bitter gourd (Momordica charantia L.), four inbred lines were crossed each other for a diallel cross set, and grown in the green house. The characters investigated were fruit length, fruit width, fruit weight, number of fruit, thickness of fruit flesh, days to maturity(from flowering), seed length, seed width, seed weight, number of seed per fruit, ovary length, ovary width, ovary weight, peduncle length, and yield. Correlation coefficient analysis revealed that six characters i.e. fruit weight, fruit length, ovary length, peduncle length, seed width, and yield showed highly positive correlations each other. Among 15 characters, eight characters, such as ovary length, ovary width, ovary weight, seed length, seed width, seed weight, number of seed, and peduncle length, showed partial dominance in vr-wr graph analysis, but they showed different degree of dominance. Ovary length, seed length, seed width, and seed weight were controlled by additive rather than dominant effect of genes, and they had very high heritabilities in both broad and narrow sense, while in ovary weight, ovary width, and peduncle length, additive effect of genes was similar to dominant effect. Number of seed per fruit was governed by dominant rather than additive effect of genes. Heritabilities(h²N) in the narrow sense were low or moderate for number of seed, ovary width, and ovary weight, and that of peduncle length was high.

Rapid Multiplication of Seed Potatoes(Solanum tuberosum L.) by Inducing Microtubers In Vitro

CHOI, YOUNG WHAN,SUNG, KYUN UM,PARK, JOONG CHOON,KANG, SEONG MO,JEONG, YEON OK,KANG JUM SOON,CHO, JEOUNG LAI 경상대학교 유전공학연구소 1990 遺傳工學硏究所報 Vol.9 No.-

Experiments were carried out to develop a mass production system of microtubers of high quality from Daiji, Sumi and Namjak potatoes. More and bigger microtubers were produced as shoot culture period increased up to 8 weeks. The M. MS-2 medium was most efficient for tuberization of Daiji. With Namjak, one-half strength of organic compounds of M.MS-2 increased total number of tubers, while 1.5 times strength increased tuber sizes. The M.MS-2 medium containing 20 to 35μM KMnO_4 helped tuberization of Daiji, but not of Namjak. Prechilling of plantlets at o and 5℃ for 10 and 15 min was favorable for tuberization of Daiji. Prechilling of Namjak plantlets decreased tuber numbers, but increased mean tuber weights. Patatin accumulated within 7 and 10 days in Sumi and in Daiji, respectively, after leaf node cutting, and this coincided with visible formation of tubers. When grown in a greenhouse, microtubers produced more tubers, but their yield on a plant basis was 80% with Sumi and 54% with Daiji compared with conventional seed potatoes.