In Vitro Screening for Compounds Derived from Traditional Chinese Medicines with Antiviral Activities Against Porcine Reproductive and Respiratory Syndrome Virus

( Jia Cheng ),( Na Sun ),( Xin Zhao ),( Li Niu ),( Mei Qin Song ),( Yao Gui Sun ),( Jun Bing Jiang ),( Jian Hua Guo2 ),( Yuan Sheng Bai ),( Jun Ping He ),( Hong Quan Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8

Seventeen compounds derived from traditional Chinese medicines (TCMs) were tested for their antiviral activity against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. Visualization with the cytopathologic effect (CPE) assay and the 3-(4, 5-dimethyithiazol- 2-yl)-2,5-diphenyltetrazolium bromide test were used to determine the 50% cytotoxic concentration (CC50) and 50% effective concentration (EC50) in cultured Marc-145 cells. Among the tested compounds, chlorogenic acid and scutellarin showed potential anti-PRRSV activity. The EC50 values were 270.8 ± 14.6 μg/ml and 28.21 ± 26.0 μg/ml and the selectivity indexes were >5.54 and 35.5, respectively. The time-of-addition and virucidal assay indicated that the anti-PRRSV activity of the two compounds could be due to their inhibiting the early stage of virus replication and/or inactivating the virus directly. The inhibition of the virus attachment was not observed in the adsorption inhibition assay. The inhibition ratios of chlorogenic acid and scutellarin were, respectively, 90.8% and 61.1% at the maximum non-cytotoxic concentrations. The results have provided a basis for further exploration of their antiviral properties and mechanisms in vivo. We believe that the chlorogenic acid and scutellarin have a great potential to be developed as new anti-PRRSV drugs for clinical application.

The effect of cyfluthrin on testis inhibin B in rats and the intervention of Lycium barbarum polysaccharide

Guo Xin,Xie Yong-Xin,Guo Chen,Wei Jing-Lin,Yang Hui-Fang 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.2

Background In recent years, studies have shown that cyfluthrin is harmful to the reproductive system. Lycium barbarum polysaccharide may be a protective agent. Objective To investigate the effect of cyfluthrin (Cy) on testosterone B (inhibin B, INHB) in male rats and the intervention of Lycium barbarum polysaccharide (LBP). Results Compared with the control group, the mRNA and protein expression levels of INH α and INH βB in the testis tissue decreased, and the difference was statistically significant (P < 0.05). The LBP intervention group can significantly increase the expression level, and the difference is statistically significant (P < 0.05). Conclusion The decrease of INHα and INHβB expression caused by Cy exposure indicates that Cy has a toxic effect on the testis of rats, and the addition of LBP has a certain protective effect on the testis of rats. Background In recent years, studies have shown that cyfluthrin is harmful to the reproductive system. Lycium barbarum polysaccharide may be a protective agent. Objective To investigate the effect of cyfluthrin (Cy) on testosterone B (inhibin B, INHB) in male rats and the intervention of Lycium barbarum polysaccharide (LBP). Results Compared with the control group, the mRNA and protein expression levels of INH α and INH βB in the testis tissue decreased, and the difference was statistically significant (P < 0.05). The LBP intervention group can significantly increase the expression level, and the difference is statistically significant (P < 0.05). Conclusion The decrease of INHα and INHβB expression caused by Cy exposure indicates that Cy has a toxic effect on the testis of rats, and the addition of LBP has a certain protective effect on the testis of rats.

Sick sinus syndrome associated with Erdheim- Chester disease was reversed by interferon-alpha treatment

( Xinli Guo ),( Yuanwei Xu ),( Ke Wan ),( Yucheng Chen ) 대한내과학회 2022 The Korean Journal of Internal Medicine Vol.37 No.1

Analysis of Factors Influencing Communication Popularity Based on Short Video Information Sources: Audience Trust as a Mediated Research Review

Xin Guo(Guo Xin ),Fang Deng(Deng Fang ) 동북아경상학회 2022 동북아경상연구 Vol.3 No.1

Purpose – In the online environment, the group definition, marginal scope, and transmission methods of short video creators and audiences have all changed, but the centrality of the audiences’ essence will not change. Their thinking characteristics and behaviors ultimately determine the influence of digital products spread in the network. This paper attempts to explore the relationship between audience trust and communication mechanism from the source of information: the trust or suspicion generated by the creator’s identity type information, and explore its influence on the popularity of communication. Design/Methodology/Approach – We systematically reviews the connotation and extension of audience trust in online media, definition and characteristics of network identity, the disclosure of creator identity types, character setting, authentication and supervision of identity, and communication popularity. Findings – The interaction between short video media and audience includes: information dissemination, value judgment and emotional transmission. Audience trust plays a bridge and check position in the dissemination of short video media, which determines the survival and development of short video media. These contents can provide short video creators with basic information that can effectively affect user behavior, provide them with a basis for decisionmaking in the creation process, increase audience trust, and improve the popularity and effectiveness of media communication. Research Implications – It provides a new research perspective for influencing user identification, social recommendation and other fields.

Simultaneous distillation-extraction for manufacturing ultra-high-purity electronic-grade octamethylcyclotetrasiloxane (D4)

Wenhui Guo,Shuhu Guo,Xu Zhao,Zhenjun Yuan,Yu Zhao,Xin Chang,Hong Li,Xiong Zhao,Ye Wan,Dazhou Yan,Zhongyuan Ren,Xiaolei Fan,Xin Gao 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.109 No.-

Ultra-high-purity (UHP) electronic-grade octamethylcyclotetrasiloxane (D4) is the key precursor of lowdielectricconstant (low-k) SiCOH films to manufacture integrated circuits (IC), meeting the stringentrequirements of the rapidly developing semiconductor industry. Commonly, metallic impurities in D4were removed by multiple unit operations of adsorption, filtration, and distillation, which could reducethe concentration of a single metallic impurity below 1 ppb. However, D4 with higher purity is requiredby semiconductor production due to an increase in transistor density. Herein, a novel method based onthe integrated simultaneous distillation–extraction (SDE) was developed for manufacturing UHPelectronic-grade D4. The lab and pilot scale experiments showed that the purity of water and D4 has apositive correlation. Based on the experimental data, a double-column process, consisting of azeotropic/extractive distillation column and precision distillation column with UNIQUAC method, was establishedto access the feasibility of scaling up the SDE process. According to the simulation results, D4with the purity > 99.999 wt.% and total metallic impurities (TMI) content below 1 ppb could be obtainedusing ultra-pure water.

Genome-wide identification, evolution and expression analysis of the FtsH gene during fruit development in pear (Pyrus bretschneideri)

Guo Zhihua,Gao Xin,Cai Huaqing,Yu Lu,Gu Chao,Zhang Shao Ling 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.4

Filtration temperature-sensitive H (FtsH) is an ATP-dependent protease family that plays important roles in many aspects of cellular processes and environmental adaption. However, little information is available about the FtsH genes in pear (Pyrus bretschneideri). In this study, 19 PbrFtsH genes were identified and classified into eight groups with several subgroups. The FtsH genes in pear show high sequence and structural conservation in the coding regions, and the majority of the FtsH members were located on the chromosome 3 (three members), chromosome 9 (four members), and chromosome 15 (three members). The WGD/segmenta, dispersed duplication played a major driving force in pear FtsH evolution. A Synteny analy- sis provided deep insight into the evolutionary relationships of PbrFtsH genes. The subcellular localization of the PbrFtsH genes showed them in the mitochondrion or chloroplast. Expression profiles derived from transcriptome data and RT-qPCR analysis revealed distinct expression patterns of PbrFtsH genes in different fruit developmental stages. These results provided valuable information that extends our understanding of the function, evolution and expression profile of the FtsH family during fruit development in pear, and a foundation for the further functional characterization of PbrFtsH genes, which can be applied to pear crop improvement.

Pesticide-induced changes in fecundity and rice stripe virus transmission ability in Laodelphax striatellus (Homoptera: Delphacidae)

Guo-Qing Yang,Xin Gao,Nan-Nan Zhang,Dan-Yu Chen,Fang Liu,Jian-Xiang Xu,Jin-Cai Wu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.3

The widespread use of pesticides in rice has caused the resurgence of the small brown planthopper (SBPH), Laodelphax striatellus. In addition to inciting damage by feeding on phloem cells, SBPH also functions as a vector for rice stripe virus (RSV), which can cause serious yield losses. In general, studies focused on pesticide-induced stimulation of SBPH populations have focused on the insects; little information is available on the impact of pesticides on RSV transmission by SBPH. The present study investigated the effects of two pesticides (validamycin and triazophos) on the fecundity and RSV transmission ability of SBPH. Our results demonstrated that the fecundity of non-viruliferous (naïve) or viruliferous SBPH was enhanced by exposure to triazophos at the LC20 and validamycin at 200 mg L−1. Meanwhile, the increased number of eggs produced by viruliferous females treated with pesticides was larger than those from naïve females. Exposure to validamycin caused an increase in horizontal transmission of RSV; furthermore, vertical viral transmission rates of SBPH were significantly increased by exposure to triazophos. The present study provides valuable information for understanding the mechanisms underlying the resurgence of SBPH and subsequent outbreaks of RSV.

Estimating the Probability Density Function of Remaining Useful Life for Wiener Degradation Process with Uncertain Parameters

Guo Xie,Xin Li,Xi Peng,Fucai Qian,Xinhong Hei 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.11

The effective prediction of remaining useful life is essential to realize system failure diagnosis and health management. The existing researches often assume that the degradation model is constant or the degradation process is measurable. The accurate degradation model, however, usually can not be established, and the parametric variation and measurement error of the degradation process are unavoidable, which makes it hard to obtain the exact value for predicting the remaining useful life. Regarding this problem, on basis of the concept of first failure time, a real-time probability density function is derived for the Wiener degradation process with the uncertainty of parameters, the stochasticity of degradation process and the randomness of measurement error. The main steps are as follows: firstly, the degradation model with three kinds of uncertainties is established, and then the stochastic degradation state and the parameters of the uncertainty model are estimated by fusion Kalman/UFIR filter; then, the analytical expression of the probability density function of remaining useful life is deduced. Finally, the correctness and effectiveness of the proposed method are verified by a group of comparison experiments and Monte Carlo simulations.

The Antihydrolytic Effect and Properties of Crosslinked Polyurethane Containing Natural Dimer Fatty Acids Building Blocks

Xin Liu,Kai Xu,Huan Liu,Hualun Cai,Zien Fu,Ying Guo,Mingcai Chen 한국고분자학회 2012 Macromolecular Research Vol.20 No.6

Four series of cross-linked polyurethanes with polyester polyols as soft segments were prepared by selfcuring of isocyanate-terminated polyurethanes. Polyester polyols herein were derived from different dimer fatty acids weight content, such as dicarboxylic acid leading to various hydrophobicity and steric hindrance. The structure of cross-linked polyurethane was characterized by Fourier transform infrared spectroscopy (FTIR). The surface properties and mechanical and thermal properties of cross-linked polyurethane were investigated using contact meter, tensile tester, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). The results indicated that with increasing dimer fatty acid weight content from 0% to 100%, the cross-linked polyurethanes exhibited increased hydrophobicity (water contact angle increased from 91o to 96o), improved water resistance (water uptake decreased from 1.41% to 0.34%), toluene resistance (weight loss decreased from 33.2% to 22.7%), and hydrolytic resistance (weight loss decreased from 0.60% to 0.27%), enhanced mechanical properties (tensile strength increased from 1.3 to 4.8 MPa), and thermal stability (decomposition temperature of 50% weight loss increased from 425 to 462 oC).

On the uniform consistency of frequency polygons for ψ-mixing samples

Guo-dong Xing,Shan-chao Yang,Xin Liang 한국통계학회 2015 Journal of the Korean Statistical Society Vol.44 No.2

In this article, the frequency polygon investigated by Scott (1985) is studied as a nonparametric estimator for ψ-mixing samples. Under mild conditions, we prove the uniformly strong consistency of the estimator and obtain the corresponding rate of convergence which is nearly equal to the one obtained by Carbon et al. (1997) and, our results weaken the relevant conditions used by Carbon et al. (1997). In addition, the uniformly weak consistency is also given.