Long-term Stability after Reduction of Mandible Fracture by Keyhole Plate: Evaluation at the Time of Plate Removal

Cheon, Kyeong-Jun,Cho, Seoung-Won,Jang, Won-Seok,Kim, Ju-Won,Yang, Byoung-Eun Korean Association of Maxillofacial Plastic and Re 2020 Maxillofacial Plastic Reconstructive Surgery Vol.42 No.-

Background: Various types of miniplates have been developed and used for the reduction of facial bone fractures. We introduced Yang's Keyhole (YK) plate, and reported on its short-term stability. The purpose of this study was to evaluate the long-term stability of the YK plate, as a follow-up study, by examining the patients who had used the YK plate among the patients with the reduction of mandible fractures and who visited for plate removal. Methods: We reviewed the medical records of 16 patients who underwent mandibular fracture fixation using a YK plate (group I) and 17 patients who underwent mandibular fracture fixation using a conventional plate (group II). Assessment was then made on malunion, occlusal stability, discomfort during the application, and clinical symptoms. Results: From January 2015 to December 2017, a total of 36 patients underwent mandibular fracture surgery using a YK plate. A total of 16 patients received plate removal. Among them, 15 were male and 1 female. The average age was 26 years. The applied surgical sites were the 12 on mandibular angle, 4 on mandibular symphysis, and 2 on subcondyle. The application period of YK plate was an average of 335 days. During the same period, 45 people underwent surgery on the conventional plate. A total of 17 patients received plate removal. Among them, 15 were male and 2 females. The average age was 36 years. The applied surgical sites were the 8 on mandibular angle, 4 on mandibular symphysis, and 2 on subcondyle. The application period of the conventional plate was an average of 349 days. No malocclusion occurred at the time of removal, and occlusion was stable. No patient complained of joint disease or discomfort. Conclusion: The YK plate system, in which the screw was first inserted and the plate was applied, for clinical convenience did not cause any particular problem and no significant difference from the conventional plate.

Efficient Charge Balance of Green Organic Light Emitting Diodes Using Mixed Charge Transporting Materials as the Host

Yang, Hyung Jin,Lee, Ho Won,Lee, Seok Jae,Lee, Song Eun,Lee, Dong Hyung,Koo, Ja Ryong,Yoon, Jhin Yeong,Yoon, Seung Soo,Kim, Young Kwan Informa UK (TaylorFrancis) 2014 Molecular Crystals and Liquid Crystals Vol.599 No.1

Essential Factors in Predicting the Need for Angio-Embolization in the Acute Treatment of Pelvic Fracture with Hemorrhage

( Seok-won Yang ),( Hee-gon Park ),( Sung-hyun Kim ),( Sung-hyun Yoon ),( Seung-gwan Park ) 대한외상학회 2019 大韓外傷學會誌 Vol.32 No.2

Purpose: The purpose of this study was to determine the essential factors for prompt arrangement of angio-embolization in patients with pelvic ring fractures. Methods: A total of 62 patients with pelvic ring fractures who underwent angioembolization in Dankook University Hospital from March 2013 to June 2018 were retrospectively reviewed. There were 38 men and 24 women with a mean age of 59.8 years. The types of pelvic ring fractures were categorized according to the Tile classification. Patient variables included sex, initial hemoglobin concentration, initial systolic blood pressure, transfused packed red blood cells within 24 hours, Injury Severity Score (ISS), mortality rate, length of hospital stay, and time to angio-embolization. Results: The most common pelvic fracture pattern was Tile type B (n=34, 54.8%). The mean ISS was 27.3±10.9 with 50% having an ISS ≥25. The mean time to angio-embolization from arrival was 173.6±89 minutes. Type B (180.1±72.3 minutes) and type C fractures (174.7±91.3 minutes) required more time to angio-embolization than type A fractures (156.6±123 minutes). True arterial bleeding was identified in types A (35.7%), B (64.7%), and C (71.4%). Conclusions: It is important to save time to reach the angio-embolization room in treating patients with pelvic bone fractures. Trauma surgeons need to consider prompt arrangement of angio-embolization when encountering Tile type B or C pelvic fractures due to the high risk of true arterial bleeding.

Nitric Oxide-Induced Autophagy in MC3T3-E1 Cells is Associated with Cytoprotection via AMPK Activation

Yang, Jung Yoon,Park, Min Young,Park, Sam Young,Yoo, Hong Il,Kim, Min Seok,Kim, Jae Hyung,Kim, Won Jae,Jung, Ji Yeon The Korean Society of Pharmacology 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.6

Nitric oxide (NO) is important in the regulation of bone remodeling, whereas high concentration of NO promotes cell death of osteoblast. However, it is not clear yet whether NO-induced autophagy is implicated in cell death or survival of osteoblast. The present study is aimed to examine the role of NO-induced autophagy in the MC3T3-E1 cells and their underlying molecular mechanism. The effect of sodium nitroprusside (SNP), an NO donor, on the cytotoxicity of the MC3T3-E1 cells was determined by MTT assay and expression of apoptosis or autophagy associated molecules was evaluated by western blot analysis. The morphological observation of autophagy and apoptosis by acridine orange stain and TUNEL assay were performed, respectively. Treatment of SNP decreased the cell viability of the MC3T3-E1 cells in dose- and time-dependent manner. SNP increased expression levels of p62, ATG7, Beclin-1 and LC3-II, as typical autophagic markers and augmented acidic autophagolysosomal vacuoles, detected by acridine orange staining. However, pretreatment with 3-methyladenine (3MA), the specific inhibitor for autophagy, decreased cell viability, whereas increased the cleavage of PARP and caspase-3 in the SNP-treated MC3T3-E1 cells. AMP-activated protein kinase (AMPK), a major autophagy regulatory kinase, was activated in SNP-treated MC3T3-E1 cells. In addition, pretreatment with compound C, an inhibitor of AMPK, decreased cell viability, whereas increased the number of apoptotic cells, cleaved PARP and caspase-3 levels compared to those of SNP-treated MC3T3-E1 cells. Taken together, it is speculated that NO-induced autophagy functions as a survival mechanism via AMPK activation against apoptosis in the MC3T3-E1 cells.

Periodontal regeneration with nano-hyroxyapatite-coated silk scaffolds in dogs

Yang, Cheryl,Lee, Jung-Seok,Jung, Ui-Won,Seo, Young-Kwon,Park, Jung-Keug,Choi, Seong-Ho Korean Academy of Periodontology 2013 Journal of Periodontal & Implant Science Vol.43 No.6

Purpose: In this study, we investigated the effect of silk scaffolds on one-wall periodontal intrabony defects. We conjugated nano-hydroxyapatite (nHA) onto a silk scaffold and then seeded periodontal ligament cells (PDLCs) or dental pulp cells (DPCs) onto the scaffold. Methods: Five dogs were used in this study. Bilateral 4 mm${\times}$2 mm (depth${\times}$mesiodistal width), one-wall intrabony periodontal defects were surgically created on the distal side of the mandibular second premolar and the mesial side of the mandibular fourth premolar. In each dog, four of the defects were separately and randomly assigned to the following groups: the PDLCcultured scaffold transplantation group (PDLC group), the DPC-cultured scaffold transplantation group (DPC group), the normal saline-soaked scaffold transplantation group, and the control group. The animals were euthanized following an 8-week healing interval for clinical, scanning electron microscopy (SEM), and histologic evaluations. Results: There was no sign of inflammation or other clinical signs of postoperative complications. The examination of cellseeded constructs by SEM provided visual confirmation of the favorable characteristics of nHA-coated silk scaffolds for tissue engineering. The scaffolds exhibited a firm connective porous structure in cross section, and after PDLCs and DPCs were seeded onto the scaffolds and cultured for 3 weeks, the attachment of well-spread cells and the formation of extracellular matrix (ECM) were observed. The histologic analysis revealed that a well-maintained grafted volume was present at all experimental sites for 8 weeks. Small amounts of inflammatory cells were seen within the scaffolds. The PDLC and DPC groups did not have remarkably different histologic appearances. Conclusions: These observations indicate that nHA-coated silk scaffolds can be considered to be potentially useful biomaterials for periodontal regeneration.

Study on a Fixed Bed Gasification of Polyurethane SRF from Electronic Waste

( Won-seok Yang ),( Jang-soo Lee ),( Se-won Park ),( Jae-jun Kang ),( Tanvir Alam ),( Yong-chil Seo ) 한국폐기물자원순환학회(구 한국폐기물학회) 2015 한국폐기물자원순환학회 3RINCs초록집 Vol.2015 No.-

With expanding economic growth, the consumption and changing period of electronics products have increased rapidly last a couple of decades in Korea. A refrigerator generally contains 10% of polyurethane. Since the amount of used refrigerators collected at recycling facilities of E-wastes has increased since 2007, the generation of polyurethane waste has also increased. Polyurethane recycling technologies have been investigated such as polyol production and sound-absorbing materials, which are not commercialized yet due to high cost of the technology. However many kinds of plastic wastes are being used as solid refuse fuel (SRF) like RDF, so polyurethane in E-waste could be utilized as valuable fuels, since it has higher heating values and contains low sulfur, low chlorine, and less hazardous substances. In this study an attempt to fabricate pellet type of SRF from polyurethane waste was conducted to use them as fuels even though it was not easy to make pellet, because characteristics of polyurethane were high volume and low density. Recently, Korean Association of Electronics Environment (KAEE) is producing pelletized SRF. The basic characteristics of polyurethane such as elemental composition, thermal properties and proximate analysis were carried out to assess whether they could be used as fuels or not. Using a fixed bed test reactor, gasification experiments were also conducted at the temperature of 1,000 ℃ by measuring exhaust gas compositions and gas pollutants.

Identification of Skeletal Deformities in Far Eastern Catfish, Silurus asotus under Indoor Aquaculture Condition

Won Seok Yang,Hyun Woo Gil,Gwang Yeol Yoo,In-Seok Park 한국발생생물학회 2015 발생과 생식 Vol.19 No.3

For the 2 years of farming, at the indoor circulating aquaculture system, four kinds of skeletal deformities were found among 60 Far Eastern catfish, Silurus asotus. Deformities saw jawbone’s luxation, abnormality of upper lip and malocclusion. Spinal deformity was most fatal deformities with low weight and small length. Jawbone’s luxation had 1 maxilla and 2 mandibles. Abnormality of upper lip had just lip was back over. Malocclusion’s left maxilla and right maxilla were not balanced. This experiment was any deformities in this species through the deformity can grasp how it affects.

Harnessing an RNA-mediated chaperone for the assembly of influenza hemagglutinin in an immunologically relevant conformation

Yang, Seung Won,Jang, Yo Han,Kwon, Soon Bin,Lee, Yoon Jae,Chae, Wonil,Byun, Young Ho,Kim, Paul,Park, Chan,Lee, Young Jae,Kim, Choon Kang,Kim, Young Seok,Choi, Seong Il,Seong, Baik Lin Federation of American Societies for Experimental 2018 The FASEB Journal Vol.32 No.5

<P>A novel protein-folding function of RNA has been recognized, which can outperform previously known molecular chaperone proteins. The RNA as a molecular chaperone (chaperna) activity is intrinsic to some ribozymes and is operational during viral infections. Our purpose was to test whether influenza hemagglutinin (HA) can be assembled in a soluble, trimeric, and immunologically activating conformation by means of an RNA molecular chaperone (chaperna) activity. An RNA-interacting domain (RID) from the host being immunized was selected as a docking tag for RNA binding, which served as a transducer for the chaperna function for <I>de novo</I> folding and trimeric assembly of RID-HA1. Mutations that affect tRNA binding greatly increased the soluble aggregation defective in trimer assembly, suggesting that RNA interaction critically controls the kinetic network in the folding/assembly pathway. Immunization of mice resulted in strong hemagglutination inhibition and high titers of a neutralizing antibody, providing sterile protection against a lethal challenge and confirming the immunologically relevant HA conformation. The results may be translated into a rapid response to a new influenza pandemic. The harnessing of the novel chaperna described herein with immunologically tailored antigen-folding functions should serve as a robust prophylactic and diagnostic tool for viral infections.—Yang, S. W., Jang, Y. H., Kwon, S. B., Lee, Y. J., Chae, W., Byun, Y. H., Kim, P., Park, C., Lee, Y. J., Kim, C. K., Kim, Y. S., Choi, S. I., Seong, B. L. Harnessing an RNA-mediated chaperone for the assembly of influenza hemagglutinin in an immunologically relevant conformation.</P>

Matrix metalloproteinase‐1 induces cleavage of exogenous alphab‐crystallin transduced by a cell‐penetrating peptide

Yang, Seung Won,Lee, Seung‐,Min,Choi, Eun Young,Lee, Kyung Hye,Kim, Soo Hyuk,Shin, Min‐,Jeong,Han, Ye Sun,Kang, Seok‐,Min,Chung, Ji Hyung Wiley Subscription Services, Inc., A Wiley Company 2011 Journal of cellular biochemistry Vol.112 No.9

<P><B>Abstract</B></P><P>Cell‐penetrating peptides (CPPs), including TAT‐CPP, have been used to deliver exogenous proteins into living cells. Although a number of proteins fused to TAT‐CPP can be delivered into various cells, little is known about the proteolytic cleavage of TAT‐fusion proteins in cells. In this study, we demonstrate that a small heat shock protein (sHSP), alphaB‐crystallin (αB‐crystallin), delivered by TAT‐CPP is susceptible to proteolytic cleavage by matrix metalloproteinase‐1 (MMP‐1) in cardiac myoblast H9c2 cells. Recombinant TAT‐αB‐crystallin was efficiently transduced into H9c2 cells. For a few hours following protein transduction, generation of a 14‐kDa fragment, a cleavage band of TAT‐αB‐crystallin, increased in a time‐dependent manner. This fragment was observed only in detergent‐insoluble fractions. Interestingly, treatment with MMP inhibitors blocked the cleavage of TAT‐αB‐crystallin. In test tubes, recombinant MMP‐1 processed TAT‐αB‐crystallin to generate the major cleavage fragment 14‐kDa, as observed in the cells treated with TAT‐αB‐crystallin. The N‐terminal sequences of the 14‐kDa fragment were identified as Leu‐Arg‐Ala‐Pro‐Ser‐Trp‐Phe, indicating that this fragment is generated by cleavage at Phe54‐Leu55 of αB‐crystallin. The MMP‐1‐selective inhibitor abolished the production of 14‐kDa fragments in cells. In addition, the cleaved fragment of TAT‐αB‐crystallin was significantly reduced in cells transfected with MMP‐1 siRNA. Moreover, the enzymatic activity of MMP‐1 was markedly increased in TAT‐αB‐crystallin‐treated cells. TAT‐αB‐crystallin has a cytoprotective effect on H9c2 cells under hypoxic insult, moreover, MMP‐1‐selective inhibitor treatment led to even increased cell viability. These results suggest that MMP‐1 is responsible for proteolytic cleavage of TAT‐αB‐crystallin during its intracellular transduction in H9c2 cells. J. Cell. Biochem. 112: 2454–2462, 2011. © 2011 Wiley‐Liss, Inc.</P>

In Vitro Cytotoxic Effects of Copper Nanoparticles in Human Neuroblastoma Cells

Seok-Won JANG,Min-Su OH,Biao LI,Sung Ik YANG,Eun-Min CHO 한국생물공학회 2014 한국생물공학회 학술대회 Vol.2014 No.4