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Kang Zhi‐Wei,Liu Fang‐Hua,Xu Yong‐Yu,Cheng Jia‐Hui,Lin Xiao‐Li,Jing Xiang‐Feng,Tian Hong‐Gang,Liu Tong‐Xian 한국곤충학회 2021 Entomological Research Vol.51 No.1
Odorant‐degrading enzymes (ODEs) have been found in insect antennae and play a critical role in signal chemical degradation once the message is conveyed. Significant progress has been made in characterizing ODEs in a variety of pests but very little is known in their natural enemies. We have carried out an antennae‐ and sex‐specific transcriptome of Aphidius gifuensis, a natural enemy of aphid, to identify the candidate ODEs. Based on the antennae‐ and sex‐specific transcriptome, a total of 100 putative ODEs were identified including one aldehyde oxidase (AOX), four alcohol dehydrogenases (ADs), eight UDP‐glucuronosyltransferases (UGTs), 45 cytochrome P450 (P450s), nine glutathione S‐transferases (GSTs) and 40 carboxylesterases (CCEs or CXEs). Additionally, we used RT‐qPCR to determine the expression profiles of these genes in tissues of both sexes. Based on the phylogenic analysis and tissue‐expression patterns, AgifEstE4, AgifCXE3, AgifCCE4, AgifCCE7, and AgifCCE18 were suggested as key ODEs in A. gifuensis. In addition, the female or male specifically enriched genes, such as AgifCCE17, AgifEstB1, AgifCYP18a1, AgifUGT2C2, were also considered to involve in the chemosensory processing in A. gifuensis. This study not only identified the candidate ODEs in A. gifuensis but also provided source for further exploration of the molecular mechanisms of chemical signal transductions in A. gifuensis, as well as other hymenopteran species.
A Study on the Factors of Shared Accommodation Services
Wei-Jia Li(이위가),Zi-Yang Liu(유자양) 한국컴퓨터정보학회 2021 韓國컴퓨터情報學會論文誌 Vol.26 No.7
본 연구의 목적은 중국의 공유경제 시장에서 소비자들의 지각된 기대요인 지각된 유용성, 지각된 용이성, 지각된 즐거움, 그리고 지각된 위험과 공유숙박 서비스를 제공하는 기업들에 대한 소비자 신뢰, 그리고 공유숙박 서비스의 이용의도에 어떠한 영향을 미치고 있는지를 실증적으로 분석하고자 하는 목적이 있었다. 실증분석에 필요한 데이터는 앙케트 조사를 통해 얻으며, 신뢰성분석, 타당성분석, 상관분석, 구조방정식 분석 등 방법을 통해 실시하였다. 분석결과를 보면 지각된 유용성, 지각된 즐거움이 신뢰에 긍정적인 영향을 미쳤다. 신뢰는 이용의도에 긍적적인 영향을 미쳤다. 신뢰는 유용한 매개변수로 나타났다. 그러나 지각된 위험이 신뢰에 미치지 않은 영향을 검정하였다. 이러한 결론은 공유숙박 플랫폼 운영회사의 운영방식을 개선하고 공유숙박 산업의 발전에 조언을 제공할 것이다. This study explore the perceived differences of factors influencing the shared accommodation in China, and the influencing paths of consumers’ use of shared accommodation. This paper collects the questionnaire survey of the Chinese shared accommodation user and applies method of Amos V23.0. The results showed that perceived usefulness and perceived pleasure had positive effects on trust, and they also have indirect positive effects on perceived intention through trust, while perceived risk and perceived ease of use had no significant effects on trust. The conclusion is helpful to promote the long-term development of sharing accommodation companies by satisfying the needs of consumers from the point of view of consumers.
( Jia Wei ),( Xiao Dan Cao ),( Sheng Min Zhou ),( Chao Chen ),( Hai Jun Yu ),( Yao Zhou ),( Ping Wang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.8
Vascular endothelial growth factor (VEGF) plays a key role in angiogenesis through binding to its specific receptors, which mainly occurs to VEGF receptor 2 (VEGFR-2), a kinase insert domain-containing receptor. Therefore, the disruption of VEGFR-2 signaling provides a promising therapeutic approach for the treatment of cancer by inhibiting abnormal or tumorinduced angiogenesis. To explore this potential, we expressed the catalytic domain of VEGFR- 2 (VEGFR-2-CD) as a soluble active kinase in Escherichia coli. The recombinant protein was purified and the VEGFR-2-CD activity was investigated. The obtained VEGFR-2-CD showed autophosphorylation activity and phosphate transfer activity comparable to the commercial enzyme. Furthermore, the IC50 value of known VEGFR-2 inhibitor was determined using the purified VEGFR-2-CD. These results indicated a possibility for functional and economical VEGFR-2-CD expression in E. coli to use for inhibitor screening.
Jia, Xiangling,Zhang, Chen,Liu, Juanjuan,Lv, Wei,Wang, Da-Wei,Tao, Ying,Li, Zhengjie,Zheng, Xiaoyu,Yu, Jong-Sung,Yang, Quan-Hong The Royal Society of Chemistry 2016 Nanoscale Vol.8 No.8
<P>A controllable drying strategy is proposed for the precise and non-destructive control over the structure of a 3D graphene assembly. Such an assembly is used as a model carbon material to investigate the pore structure-dependent shuttle effect and cycling performance of the cathode of a Li-S battery.</P>