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Renlong Xiong,Yi Liu,Haitao Si,Huabei Peng,Shanling Wang,Binhan Sun,Hanxin Chen,Hyoung Seop Kim,Yuhua Wen 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.10
In order to improve the work hardening capacity under low stresses and the yield strength of conventional Hadfield steels, theeffects of Si on the microstructure and work hardening behavior of the Fe‒17Mn‒1.1C‒xSi steels under both quasi-statictensile and low load impact are investigated. It is shown that the increase of the Si contents remarkably improves the yieldstrength by 36 MPa per 1 wt% Si in the investigated steel system without significant sacrifice of ductility. The decreasingeffect of Si on the stacking fault energy is strongly affected by carbon, although the variation of carbon content was small. This led to the unexpected similar stacking fault energy between 1Si and 2Si steel. With the increase of the Si contents forthe steels, the critical strain for the onset of mechanical twinning was lowered, which was controlled by the cooperationbetween the stacking fault energy and solid solution strengthening of Si. This resulted in the earlier initiation of mechanicaltwins and an increase in the twin volume fraction. Therefore, the work hardening capacities under both quasi-static tensileand low load impact tests were enhanced. It was also found that the impact deformation decreased as more mechanical twinsabsorbed the impact energy.
Si‐Liang Wang,Zi-Xiang YANG,Pu YANG,Chuan Xi Zhang 한국곤충학회 2016 Entomological Research Vol.46 No.5
The horned gall aphid, Schlechtendalia chinensis Bell, is the most economically valuable galling aphid. It accounts for approximately 75% of Chinese gallnut production, which is important for medical and chemical purposes. The horned gall aphid feeds on the Chinese sumac tree, Rhus chinensis, during the summer, induces galls, and overwinters on the moss Plagiomnium spp. However, the sequence information of S. chinensis is limited, and the functional gene expression changes during host alternation remain obscure. In this study, the transcriptome of the horned gall aphid was sequenced using high‐throughput sequencing technology. Approximately 49 million reads were obtained and were assembled into 99 366 transcripts and 47 400 unigenes. Annotation was performed using a BLAST search against primary databases such as NCBI nr and Swiss‐Prot, as well as enrichment analysis by GO, KOG and KEGG. Differentially expressed genes between spring and autumn migrant aphids were compared, and the expression levels of many genes related to detoxification, digestion and other functional genes were found to have changed, reflecting the adaptation ability of horned gall aphids in different seasons and hosts. Functional genes in wax biosynthesis were upregulated in spring migrant aphids. This study analyzed the expression profile difference between spring and autumn migrant aphids to approach the real functional genes change during host alternation. The transcriptome provides a basis to understanding the functional genes during gall aphid host alternation and the related physiological and molecular characteristics.
이지연,양시용,고은지,이승구,김시관,조왕식,송민동 建國大學校 自然科學硏究所 2002 建國自然科學硏究誌 Vol.13 No.2
본 연구가 VRSA에 대한 신규 항생물질 생산 균주를 얻기 위하여 실시하였다. 국내외에 채취한 토양시료에서 분리한 방선균 중, 임상분리 VRSA와 돌연변이 유발 VRSA에 대해 항균성을 나타내는 JY-24를 선별하였다. 선발 균주는 원통형의 포자 모양을 갖고 있으며, Bennett's 배지에 배양시 aerial mycelium color는 흰색을 나타냈으며, reverse side color는 대체로 노란색을 나타내었다. 항균물질 생산을 위한 탄소원으로는 fructose, mannose, 질소원으로는 yeast extract가 가장 우수하였으며 glucose의 첨가로 항균 물질 생산성이 증가하는 것으로 나타났다. 최적 초기 pH는 pH 7로 나타났으며, 28 ℃, 37℃, 45 ℃의 온도에서 균이 성장하는 것으로 나타났다. 또한 배양기간에 따라 항균물질 생산성을 조사한 결과, 임상분리 균주에 대해 5일 배양 시 가장 높았으나, 3일 배양과 7일 배양 시 동일한 생산성을 나타내 생산속도가 빠르며, 안정한 물질인 것이 파악되었다. 향후 균주동정 및 항균물질 정제에 관한 연구를 수행하고자 한다. An antibiotic substance producing strain JY-24, effective to the VRSA (Vancomycin-resistant Staphylococcus aureus), was isolated from soil. The spore chain of JY-24 was cylindrical, and its sulface was smooth. The aerial mass color of the strain was white, and its reverse side color was yellow. The strain did not produce soluble pigment. For the production antibiotics, fructose, mannose and yeast extract were favorable as carbon and nitrogen sources. Optimal initial pH for the production of the antibiotic was pH 7. Accumulation of the antibiotic in the culture broth reached at maximal level after 5 days cultivation in Bennett's medium.
Predicting the Anthocyanin Content of Kyoho Grapes by Nir Spectroscopy
( Si-yun Wang ),( Suming Chen ),( Chao-yin Tsai ),( Han-chun Hsu ),( Yung-huei Chang ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
Kyoho grapes are in dark purple color. Anthocyanin plays a major role on the colors of berries, and contains antioxidant, anti-inflammatory, anticancer substance. The method of inspecting Anthocyanins so far is by either visual or destructive technique of using chemicals, but these methods could not carry out real-time accurate inspection. The aim of this study was to demonstrate the feasibility of non-destructive inspection in predicting Anthocyanin content by near-infrared spectroscopy. This study used the NIRS 6500 spectrometer ranged from 400 to 2500 nm. The prediction model of Anthocyanin content of Kyoho grapes was established by near-infrared spectroscopy and concentration of Anthocyanin. The results of this study showed the reliability and feasibility of near-infrared spectroscopy on predicting the Anthocyanin content of grapes. In the future, the Anthocyanin content can be quickly predicted by spectroscopic techniques.
Wang Si,Andrey Kim,Hoe-Kyun Shin 한국로고스경영학회 2007 한국로고스경영학회 학술발표대회논문집 Vol.2007 No.7월
During the last few years, China has developed governmental procurement, and now it starts to pay attention to public e-procurement. Because Korea has developed governmental procurement for nearly 60 years and deployed the public e-procurement for 10 years, so it has accumulated comprehensive experience. China can learn from Korean successful implementation to solve its some critical problems. Based on analysis of Korean public e-procurement, we construct public e-procurement model for the development of Chinese e-procurement. We define the objects of public e-procurement as direction indicator of its development. And based on operation system of Korean e-procurement, we design an applicable system for China. Finally, we also propose that China should establish its electronic procurement in macroeconomic and microeconomic environment.
The effect of Zn(2+) on Pelodiscus sinensis creatine kinase: unfolding and aggregation studies.
Wang, Su-Fang,Lee, Jinhyuk,Wang, Wei,Si, Yue-Xiu,Li, Caiyan,Kim, Tae-Rae,Yang, Jun-Mo,Yin, Shang-Jun,Qian, Guo-Ying Adenine Press 2013 Journal of biomolecular structure & dynamics Vol.31 No.6
<P>We studied the effects of Zn(2+) on creatine kinase from the Chinese soft-shelled turtle, Pelodiscus sinensis (PSCK). Zn(2+) inactivated the activity of PSCK (IC(50)?=?.079??.004?mM) following first-order kinetics consistent with multiple phases. The spectrofluorimetry results showed that Zn(2+) induced significant tertiary structural changes of PSCK with exposure to hydrophobic surfaces and that Zn(2+) directly induced PSCK aggregation. The addition of osmolytes such as glycine, proline, and liquaemin successfully blocked PSCK aggregation, recovering the conformation and activity of PSCK. We measured the ORF gene sequence of PSCK by rapid amplification of cDNA end and simulated the 3D structure of PSCK. The results of molecular dynamics simulations showed that eight Zn(2+) bind to PSCK and one Zn(2+) is predicted to bind in a plausible active site of creatine and ATP. The interaction of Zn(2+) with the active site could mostly block the activity of PSCK. Our study provides important insight into the action of Zn(2+) on PSCK as well as more insights into the PSCK folding and ligand-binding mechanisms, which could provide important insight into the metabolic enzymes of P. sinensis.</P>
Effect of Myofacial Release and Cryotherapy in Acute Whiplash Injury
Si Eun Park,Joon Hee Lee,Jung San Wang,Yoo Rim Choi,Joo Hyun Park,Ju Hwan Lee,Young Duk Choi,Hyun Sook Hwang,Soon Hee Kim 국제물리치료학회 2014 Journal of International Academy of Physical Ther Vol.5 No.1
The purpose of this study is to provide the case of influence of myofacial release(MFR) and cryotherapy in acute whiplash injury. The subjects were consisted of 3 adults(2 males, 1 female) who had been diagnosed with whiplash injury. The performance period was 1 weeks, 6 times and 1 time is 40min(MFR: 20min, cryotherapy: 20min). We measured neck pain(Visual analogue scale, VAS) and cervical alignment(cervical curvature & line of gravity). All measurements of each subject were measured at pre-treatment and post-treatment. The results are listed below. The neck pain(VAS) index decreased from 7.54cm to 4.11cm. The cervical curvature increased from 23.62°to 29.76°. The line of gravity decreased from 8.03mm to 3.66mm. Based on the results, it can be suggested that both MFR and cryotherapy can be used to neck pain and alignment in acute whiplash injury.