Comparison of Suppressive Effects of Demethoxycurcumin and Bisdemethoxycurcumin on Expressions of Inflammatory Mediators In Vitro and In Vivo

Guo, Lian Yu,Cai, Xing Fu,Lee, Jung-Joon,Kang, Sam-Sik,Shin, Eun-Myoung,Zhou, Hong-Yu,Jung, Ji-Won,Kim, Yeong-Shik 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.4

Demethoxycurcumin and bisdemethoxycurcumin are the main active ingredients isolated from Curcumae Longae Radix. Recent studies demonstrated that both compounds exhibit antioxidative and anti-inflammatory effects as well as effects on cancer cell lines. In this study, we compared the activities of demethoxycurcumin and bisdemethoxycurcumin, and both compounds were evaluated on lipopolysaccharide (LPS)-induced nitric oxide (NO) production, inducible nitric oxide synthase (iNOS), cycloxygenase-2 (COX-2) and nuclear factor-kappaB $(NF-{\kappa}B)$ activity in a RAW 264.7 macrophage cell line. The evaluation:results suggested that the anti-inflammatory properties of demethoxycurcumin and bisdemethoxycurcumin were attributed to the inhibition of iNOS and COX-2 expression, as initiated by the inhibition of $NF-{\kappa}B$ activity. Additionally, both of them significantly inhibited carrageenan-induced paw edema in mice. Taken together, all of the results showed that the suppressive effect of demethoxycurcumin was stronger than that of bisdemethoxycurcumin, indicating that the methoxy group had enhanced demethoxycurcumin's anti-inflammation effects.

Cimiside E Arrests Cell Cycle and Induces Cell Apoptosis in Gastric Cancer Cells

Lian Yu Guo,Eun Ji Joo,손건호,Su Jin Jeon,Sehyun Jang,신은명,Hong Yu Zhou,Yeong Shik Kim 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.10

Cimiside E was isolated from the Cimicifuga heracleifolia Komarov extract, which has been previously demonstrated to possess apoptotic action on gastric cancer cells. The IC50 value of cimiside E on gastric cancer cells for 24 h was 14.58 μM. The mechanism of apoptosis was further elucidated through western blot, RT-PCR, morphology, Annexin V-FITC/PI staining and cell cycle analysis. Cell cycle arrest was induced by cimiside E in S phase at a lower concentration (30 μM) and G2/M phase at higher concentrations (60 and 90 μM). Cimiside E mediated apoptosis through the induction of the caspase cascade for both the extrinsic and intrinsic pathways. These findings suggest that cimiside E may be an effective chemopreventive agent against cancer.

Effects of Schisandrin on Transcriptional Factors in Lipopolysaccharide- Pretreated Macrophages

Lian Yu Guo,Tran Manh Hung,배기환,김영식,Sehyun Jang,신은명,Ji Won Chung,강삼식,김현표 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.3

Schisandrin is the main active ingredient isolated from Schisandra chinensis Baill. Recent studies have demonstrated that schisandrin exhibits anti-inflammatory effects in vivo and in vitro. In this study, we examined whether the order of lipopolysaccharide (LPS) treatment affects the mechanism of schisandrin anti-inflammatory activity. We found that the antiinflammatory mechanisms are not the same depending on whether macrophages were treated with schisandrin before or after LPS. The main difference is that inhibitor kappaBα (IκBα) degradation was not inhibited when macrophages were pretreated by LPS before schisandrin and was weakly inhibited when macrophages were pretreated by schisandrin before LPS.

Comparison of Suppressive Effects of Demethoxycurcumin and Bisdemethoxycurcumin on Expressions of Inflammatory Mediators In Vitro and In Vivo

Lian Yu Guo,Xing Fu Cai,Jung Joon Lee,강삼식,신은명,Hong Yu Zhou,Ji Won Jung,김영식 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.4

Demethoxycurcumin and bisdemethoxycurcumin are the main active ingredients isolated from Curcumae Longae Radix. Recent studies demonstrated that both compounds exhibit antioxidative and anti-inflammatory effects as well as effects on cancer cell lines. In this study, we compared the activities of demethoxycurcumin and bisdemethoxycurcumin, and both compounds were evaluated on lipopolysaccharide (LPS)-induced nitric oxide (NO) production, inducible nitric oxide synthase (iNOS), cycloxygenase-2 (COX-2) and nuclear factor-kappaB (NF-κB) activity in a RAW 264.7 macrophage cell line. The evaluation:results suggested that the anti-inflammatory properties of demethoxycurcumin and bisdemethoxycurcumin were attributed to the inhibition of iNOS and COX-2 expression, as initiated by the inhibition of NF- κB activity. Additionally, both of them significantly inhibited carrageenan-induced paw edema in mice. Taken together, all of the results showed that the suppressive effect of demethoxycurcumin was stronger than that of bisdemethoxycurcumin, indicating that the methoxy group had enhanced demethoxycurcumin’s anti-inflammation effects.

Anti-inflammatory effects of schisandrin isolated from the fruit of <i>Schisandra chinensis</i> Baill

Guo, Lian Yu,Hung, Tran Manh,Bae, Ki Hwan,Shin, Eun Myoung,Zhou, Hong Yu,Hong, Yoo Na,Kang, Sam Sik,Kim, Hyun Pyo,Kim, Yeong Shik Elsevier 2008 european journal of pharmacology Vol.591 No.1

<P><B>Abstract</B></P><P>Schisandrin is the main active ingredient isolated from the fruit of <I>Schisandra chinensis</I> Baill. Recent studies have demonstrated that schisandrin exhibits anti-oxidative effects <I>in vivo</I>. In the present study, the effect of schisandrin on plasma nitrite concentration in lipopolysaccharide (LPS)-treated mice was evaluated. It also significantly inhibited carrageenan-induced paw edema and acetic acid-induced vascular permeability in mice. Furthermore, schisandrin had a protective effect on lipopolysaccharide (LPS)-induced sepsis. <I>In vitro</I>, our results are the first that show that the anti-inflammatory properties of schisandrin result from the inhibition of nitric oxide (NO) production, prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) release, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression, which in turn results from the inhibition of nuclear factor-kappaB (NF-κB), c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) activities in a RAW 264.7 macrophage cell line.</P>

A numerical study on the correlation between the evolution of propeller trailing vortex wake and skew of propellers

Lian-Zhou Wang,Chun-Yu Guo,Yu-Min Su,Tie-Cheng Wu 대한조선학회 2018 International Journal of Naval Architecture and Oc Vol.10 No.2

The characteristics of the relationship between the evolution of propeller trailing vortex wake and skew angle are numerically examined based on four different five-blade David Taylor Model Basin (DTMB) model propellers with different skew angles. Numerical simulations are based on Reynolds-averaged NaviereStokes (RANS) equations combined with SST k u turbulence model. Results show that the contraction of propeller trailing vortex wake can be restrained by increasing skew angle and loading conditions, and root vortices fade away when the propeller skew angle increases. With the increase of the propeller's skew angle, the deformation of the hub vortex and destabilization of the tip vortices are weakening gradually because the blade-to-blade interaction becomes weaker. The transition trailing vortex wake from stability to instability is restrained when the skew increases. Furthermore, analyses of tip vortice trajectories show that the increasing skew can reduce the difference in trailing vortex wake contraction under different loading conditions.

Bacillus subtilis improves antioxidant capacity and optimizes inflammatory state in broilers

Yu Zhang,Junyan Zhou,Linbao Ji,Lian Zhang,Liying Zhao,Yubing Guo,Haitao Wei,Lin Lu Asian Australasian Association of Animal Productio 2024 Animal Bioscience Vol.37 No.6

Objective: Bacillus subtilis, a kind of probiotic with broad-spectrum antibacterial function, was commonly used in livestock and poultry production. Recent research suggested that Bacillus subtilis may have antioxidant properties and improve immune response. This study aimed to verify the probiotic function of Bacillus subtilis in the production of broiler chickens. Methods: A total of 324 (1-day-old) Arbor Acres broilers were selected and randomly divided into three groups: basal diet group (Ctr Group), basal diet + antibiotic growth promoter group (Ctr + AGP) and basal diet + 0.5% Bacillus subtilis preparation group (Ctr + Bac). The experiment lasted for 42 days. Muscle, serum and liver samples were collected at 42 days for determination. Results: The results showed that Bacillus subtilis could decrease malondialdehyde content in the serum and liver (p<0.05) and increase superoxide dismutase 1 mRNA expression (p<0.01) and total superoxide dismutase (p<0.05) in the liver. In addition, compared with AGP supplementation, Bacillus subtilis supplementation increased interleukin-10 (IL-10) and decreased tumor necrosis factor-α and IL-1β level in the serum (p<0.05). At 45 minutes after slaughter Ctr + Bac presented a higher a^* value of breast muscle than Ctr Group (p<0.05), while significant change in leg muscle was not identified. Moreover, there was no difference in weight, shear force, cooking loss and drip loss of breast and leg muscle between treatments. Conclusion: Our results demonstrate that Bacillus subtilis in diet can enhance antioxidant capacity and optimize immune response of broilers.

Association of XPD and XRCC1 Genetic Polymorphisms with Hepatocellular Carcinoma Risk

Guo, Lian-Yi,Jin, Xu-Peng,Niu, Wei,Li, Xiao-Fei,Liu, Bao-Hai,Wang, Yu-Lin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Aim: XRCC1 and XPD are two major repair genes involved in nucleotide excision repair (NER), which is reported to be associated with risk of several cancers. We explored the association of XRCC1 and XPD polymorphisms with the risk of HCC. Methods: A total of 410 cases with HCC and 410 health controls were collected. XRCC1 Arg194Trp, XRCC1 Arg399Gln, XPD Lys751Gln and XPD Asp312Asn genotyping was performed by duplex polymerase-chain-reaction with the confronting-two-pair primer (PCR-CTPP) method. Results: XRCC1 194Trp/Trp was strongly significantly associated with an increased risk of HCC cancer when compared with the wide-type genotype (OR=2.26, 95% CI=(1.23-5.38). Individuals carrying the XRCC1 399Gln/Gln showed increased risk of HCC (OR=1.74, 95%CI=1.06-2.74). The XPD 751Gln/Gln and Gln allele genotype were associated with strong elevated susceptibility to HCC (OR=3.51 and 1.42, respectively). Conclusion: These results suggest that polymorphisms in XRCC1 and XPD may have functional significance in risk of HCC.

TAZ as a novel regulator of oxidative damage in decidualization via Nrf2/ARE/Foxo1 pathway

Yu Hai-Fan,Zheng Lian-Wen,Yang Zhan-Qing,Wang Yu-Si,Wang Ting-Ting,Yue Zhan-Peng,Guo Bin 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

TAZ, as a crucial effector of Hippo pathway, is required for spermatogenesis and fertilization, but little is known regarding its physiological function in uterine decidualization. In this study, we showed that TAZ was localized in the decidua, where it promoted stromal cell proliferation followed by accelerated G1/S phase transition via Ccnd3 and Cdk4 and induced the expression or activity of stromal differentiation markers Prl8a2, Prl3c1 and ALP, indicating the importance of TAZ in decidualization. Knockdown of TAZ impeded HB-EGF induction of stromal cell proliferation and differentiation. Under oxidative stress, TAZ protected stromal differentiation against oxidative damage by reducing intracellular ROS and enhancing cellular antioxidant capacity dependent on the Nrf2/ARE/Foxo1 pathway. TAZ strengthened the transcriptional activity of Nrf2 which directly bound to the antioxidant response element (ARE) of Foxo1 promoter region. Additionally, silencing TAZ caused accumulation of intracellular ROS through heightening NOX activity whose blockade by APO reversed the disruption in stromal differentiation. Further analysis revealed that TAZ might restore mitochondrial function, as indicated by the increase in ATP level, mtDNA copy number and mitochondrial membrane potential with the reduction in mitochondrial superoxide. Additionally, TAZ modulated the activities of mitochondrial respiratory chain complexes I and III whose suppression by ROT and AA resulted in the inability of TAZ to defend against oxidative damage to stromal differentiation. Moreover, TAZ prevented stromal cell apoptosis by upregulating Bcl2 expression and inhibiting Casp3 activity and Bax expression. In summary, TAZ might mediate HB-EGF function in uterine decidualization through Ccnd3 and ameliorate oxidative damage to stromal cell differentiation via Nrf2/ARE/Foxo1 pathway.

A numerical study on the correlation between the evolution of propeller trailing vortex wake and skew of propellers

Wang, Lian-Zhou,Guo, Chun-Yu,Su, Yu-Min,Wu, Tie-Cheng The Society of Naval Architects of Korea 2018 International Journal of Naval Architecture and Oc Vol.10 No.2

The characteristics of the relationship between the evolution of propeller trailing vortex wake and skew angle are numerically examined based on four different five-blade David Taylor Model Basin (DTMB) model propellers with different skew angles. Numerical simulations are based on Reynolds-averaged Naviere-Stokes (RANS) equations combined with SST $k-{\omega}$ turbulence model. Results show that the contraction of propeller trailing vortex wake can be restrained by increasing skew angle and loading conditions, and root vortices fade away when the propeller skew angle increases. With the increase of the propeller's skew angle, the deformation of the hub vortex and destabilization of the tip vortices are weakening gradually because the blade-to-blade interaction becomes weaker. The transition trailing vortex wake from stability to instability is restrained when the skew increases. Furthermore, analyses of tip vortice trajectories show that the increasing skew can reduce the difference in trailing vortex wake contraction under different loading conditions.