A New Sesame Variety, “Kangbaek” with Disease Resistance, Lodging Tolerence and High Yield

Kang-Bo Shim,Churl-Whan Kang,Suk-Bok Pae,Si-Kyu Lim,Yu-Young Lee,Duck-Yong Suh,Jae-Whan Rho,Jin Song,Dong-Whi Kim,Ho-Young Kim 한국육종학회 2005 한국육종학회지 Vol.37 No.5

A new sesame variety Kangbaek was developed from Yeongnam Agricultural Research Institute in 2004. Cros wasmade by lodging resistant SP9003-3 line to the F1with high yielding capacity, folowed by pedigree selection, yield test and RYT by the sesame breeding team at the National Insti-tute of Crop Science up to 2003. The variety showed higher disease resistance, especially phytophothra disease, and lodging resis-respectively. It's 1000 grains weight was about 2.54 g indicating 0.03 g heavier than that of Yangbaekkae, and it's oil content wasabout 52%. Kangbaek also contained total 7.65mg/g of such lignans as sesamin and sesamolin. The average yield of Kang-baek was 82 kg per 10a at the national-wide regional performance.

Aedes aegypti 난소의 Malate Dehydrogenase Isozyme에 관한 연구

姜奭熙,金仁圭 성균관대학교 기초과학연구소 1986 論文集 Vol.37 No.1

Aedes aegypti 암모기가 흡혈후 72시간 사이에 완료하게 되는 난성숙과정에서 malate dehydrogenase(MDH)의 활성도 및 동위효소 양상에 관하여 연구하였다. MDH 활성도는 흡혈후 34시간에 상승하기 시작하여 44시간에는 절정에 이르렀으며 이 상승된 활성도는 난성숙이 완료되는 72시간동안 그대로 지속되었다. 흡혈하지 않은 암모기 성충의 MDH 동위효소를 조사한 결과 세가지 동위효소 즉 MDH-1, MDH-2, 그리고 MDH-3이 관찰되었다. 그러나 흡혈한 암모기에서는 새로운 제 4 의 동위효소 MDH-4가 관찰되었다. MDH-4는 흡혈후 18시간부터 나타나기 시작하여 48시간까지의 사이에 계속 양적으로 증가함이 관찰되었다. 흡혈한 암모기의 체내기관내의 분포를 조사한 결과 MDH-4는 난소에서만 존재함이 발견되었고 특히 난소내에서도 mitochondria 구획에서만 나타났다. 또 MDH-4는 반드시 흡혈후에 나타났으므로 흡혈행위의 지배하에서 합성되는 것으로 추정될 수 있다. 유충과 번데기 그리고 흡혈하지 않은 암모기에서 추출된 MDH의 L-malate에 대한 K_m은 흡혈후 72시간된 암모기의 난소에서 추출된 MDH의 K_m 보다 높은 것이 관찰되었다. 그러나 흡혈후 72시간에 얻은 낮은 MDH의 K_m이 MDH-4 때문인지 아닌지에 관해서는 충분한 조사는 없다. Malate dehydrogenase(MDH)(EC.1.1.1.37) isozymes from the anautogenous mosquito, Aedes aegypti, have been examined. The MDH activity in the blood fed adult females began to rise at 34 hours after the blood meal to reach at the maximal level at 44 hours, and then he activity remained at that level until the end of vitellogenesis. The unfed adult females contained three MDG isozymes, designated as MDH-1, MDH-2, MDH-3. The MDH isozyme patterns of larvae and pupae were found to be identical to that of the unfed adult female. In the blood fed females, MDH-4 began to appear at 18 hours post blood meal, which continued to rise through 48 hours, hours, MDH-4 was localized only in the ovarian mitochondrial fractions of the blood fed adult females, suggesting the possibility that the synthesis of this isozyme may be influenced by the blood feeding activity. The K_m for L-malate of the MDH isozymes isolated from the females at 72 hours post blood meal(K_m=1.97×10exp(-3)M) was found to be lower than that isolated from the non-blood fed females (K_m=4.57×10exp(-3)M), the larvae(K_m=3.91×10exp(-3)M, 3.72×10exp(-3)M), and the pupae(K_m=3.30×10exp(-3)M). The physiological role of this MDH-4 on the ovarian development and egg maturation in Aedes mosquitoes are not known.

포르피린증 환자에서 보인 가역적 자기공명영상소견 1례

강석윤,마효일,곽규환,이상암 대한신경과학회 1998 대한신경과학회지 Vol.16 No.2

백색부후균 전처리에 의한 목재 칩의 Biopulping

강규영,김형진,양봉숙,조병묵,오정수 동국대학교 생명자원과학대학 연습림 2000 연습림논문집 Vol.- No.6

Pulping process is to mainly obtain lignocellulosic fibers for papermaking from wood resources. Three types of pulping method have been basically employed, such as chemical pulping, mechanical pulping, and their combination methods. Biopulping technique has been mainly applied in mechanical pulping by pretreatment of white-rot fungi into the ultrastructure of wood cell wall. The main purpose of biopulping is to improve the pulp qualities, paper properties, and to reduce the energy costs and environmental impact, compared to traditional pulping systems. Nowadays, biopulping is frequently tried in chemical pulping operation for environmental concept, fiber modification, improvement of physical properties of paper and economic savings. The pretreatment of white-rot fungi prior to chemical pulping could affect the swelling and softening of wood cell walls by the behaviors of modification and depolymerization of ligin compounds. This study was carried out to investigate the effectiveness of biological treatment to wood chips by white-rot fungus and its structural change of lignocellulosic fiber via kraft pulping. A rotary bioreactor was applied for pretreatment of biological action. The treatment of white-rot fungus was shown in weight loss of chips. After bio-kraft pulping, kappa number and total yield of pulps were measured, and reduced. Freeness(CSF) values were also decreased. Whereas brightness values were increased, compared to untreated control. The physical strength of handsheets was shown a slight increase in tensile, tear, burst, and folding endurance, with bio-treatments.

후복막강 부신경절종 적출시 Nicardipine과 Esmolol을 사용한 마취관리 : 증례보고 A case report

강규식,김덕,김천숙 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Paragangliomas are tumors arising from the neuroendocrine elements (Chief cells) of the paraganglia. Paragangliomas are uncommon tumors and are infrequently found in the extraadrenal retroperitoneum. We investigated and treated a patient with retroperitoneal paraganglioma, which was found incidentally at abdominal computer tomograhy. It was confirmed by pathological study. We report a case of retroperitoneal paraganglioma successfully managed intraoperatively with an infusion of nicardipine and an intermittent esmolol injection.

Streptococcus에 대한 chlorhexidine dihydrochloride와 불화나트륨의 효과

강인성,양규호,최남기,김선미,오종석 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.2

Chlorhexidine dihydrochloride와 불화나트륨은 구강 내 세균을 억제하고 치태의 형성을 억제하기 위하여 많이 사용되고 있는 물질이다. 그러나 구강에는 여러 종류의 세균들이 상주하며 이들 물질에 대한 감수성도 다르다. 본 연구에서는 chlorhexidine dihydrochloride와 불화나트륨에 의해 Streptococcus mutans의 인공치태 형성이 억제되었을 때 Streptococcus mutans, Streptococcus sobrinus, Streptococcus oralis, Streptococcus salivarius의 증식에 미치는 이들 물질의 영향을 관찰하여 다음과 같은 결과를 얻었다. Streptococcus mutans를 8시간 배양하면 와이어에 형성된 인공치태 무게는 106.1±18.1㎎이었으나, 1.0μM chlorhexidine dihydrochloride를 첨가하면 5.1±1.5㎎으로 감소되었다(p<0.05). 이 때 배양액의 흡광도도 감소되었다. Streptococcus sobrinus의 배양액 흡광도는 감소되었으나 Streptococcus oralis에서는 감소되지 않았고 Streptococcus salivarius에서는 배양 8시간에 감소되었다가 배양 24시간에 대조군과 차이가 없었다. 배지에 3.0 mM 불화나트륨을 첨가하여 Streptococcus mutans를 8시간 배양하면 와이어에 인공치태 무게는 26.7±8.3㎎으로 감소되었다(p<0.05). 이 때 배양액의 흡광도도 감소되었다. Streptococcus sobrinus와 Streptococcus oralis에서의 배양액의 흡광도도 감소되었으나 Streptococcus salivarius에서는 거의 감소되지 않았다. 이상의 결과는 Streptococcus mutans의 인공치태 형성을 억제하는 chlorhexidine dihydrochloride와 불화나트륨 농도에서 Streptococcus의 여러 종의 감수성이 각각 다르다는 것을 시사하였다. Chlorhexidine dihydrochloride and sodium fluoride have been used as agents inhibiting the replication of oral bacteria and the formation of dental plaque. There are various kinds of bacteria with different sensitivity against these agents. In this study, chlorhexidine dihydrochloride and sodium fluoride were studied about their effects on the replication of Streptococcus mutans, Streptococcus sobrinus, Streptococcus oralis and Streptococcus salivarius at their concentrations inhibiting the formation of artificial plaque by Streptococcus mutans. When Streptococcus mutans was cultured for 8 hours in the media added with l.0μM chlorhexidine dihydrochloride, the weight of formed artificial plaque was decreased to 5.1 ±1.5mg compared with 106.1±18.1mg of the control(p〈0.05). At the same time, the optical density of cultured media was decreased. The optical density of cultured media was slightly decreased in Streptococcus sobrinus. but was not decreased in Streptococcus oralis. The optical density of Streptococcus salivarius was decreased at 8 hours-incubation, was not decreased at 24 hours-incubation. When Streptococcus mutans was cultured for 8 hours in the media added with 3.0 mM sodium fluoride. the weight of formed artificial plaque was decreased to 26.7i8.3 mg(p〈0.05). At the same time. the optical density of cultured media was decreased. The optical density of cultured media was decreased in Streptococcus sobrinus and Streptococcus oralis, but was slightly decreased in Streptococcus salivarius. These results suggest that at the concentration of chlorhexidine dihydrochloride and sodium fluoridehe inhibiting the formation of artificial plaque by Streptococcus mutans, different species of genus Streptococcus show the different sensitivity against these agents.

急性骨髓性 白血症에서의 試驗管內 集落細胞 形成에 관한 연구

고석만,조덕연,박철신,강원권,김민범,김삼용,노흥규 忠南大學校 癌共同硏究所 1991 癌共同硏究所 硏究誌 Vol.1 No.1

To evaluate the in vitro granulocyte-macrophage colony formation in acute myeloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 10 patients with acute myeloblastic leukemia (AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor (CSF), and 2×105 cells/ml. Human placental conditioned medium (HPCM) and phytohemagglutinin-leukocyte conditioned medium (PHA-LCM) were used as colony stimulating factor. Colony counting was done on 7th day of culture. Colony was defined as containg 20 or more cells, and cluster was defined as containing 3 - 19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2×10^(5) cells (20±19)and that of colonies was 5-24/2×10^(5) cells (14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed 5-39/2×10^(5) cells(18±16) and that of colonies was 6-13/2×10^(5) cells (9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation : 1) non-forming 2) cluster forming 3) both cluster and colony forming. Of 10 cases, 5 cases were 'non-forming', 2 cases were 'cluster forming'; and 3cases wer cluster and 'colony forming'; 3. All 5 cases 'non-forming' cases, one of 2 cases of 'cluster forming'; and none of 3 cases of 'cluster and colony forming' achieved complete remission. So, there was significant difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.

아토피피부염 소아 청소년에서의 정신병리와 양육태도 연구

홍강의,정재석,김규한 대한소아ㆍ청소년정신의학회 1999 소아청소년정신의학 Vol.10 No.1

효소 전처리 BCTMP 시트의 노화 거동

양봉숙,김형진,강규영,조병묵,오정수 동국대학교 생명자원과학대학 연습림 2000 연습림논문집 Vol.- No.6

It was generally known that the aging treatment of paper is accompanied with the reductions in physical and optical properties of individual fibers and sheet. In particular, the paper prepared with mechanical pulps occurs the yellowing of sheet, during aging, due to the absorption of light or temperature, and finally has limitation in widely applicable functions of paper. The aging behaviors of paper can be discussed with three groups of factor. One is considered to external factors, like heat, light and moisture. Another is commented with environmental factors, like oxygen, nitrogen, sulfur, and other air pollutant materials. The other is explained by internal factors, like pulp types, lignin contents, and so on. In this paper, the aging behaviors of paper prepared with BCTMP were studied in order to know how to affect in physical and optical properties of paper. For modification of fibers properties, a commercial enzyme was pretreated in the slurry of BCTMP, before handsheet-making. Sheets were aged by accelerated heat treatment. The comparisons were carried out in mechanical strength properties and color reversions. Aging treatments were resulted in higher brightness reductions, in enzyme pretreated paper.

Streptococcus의 유당분해에 대한 자일리톨의 효과

신강호,양규호,최남기,김선미,오종석 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.2

자일리톨은 탄소 5개가 있는 탄수화물로 치아우식증을 억제할 목적으로 사용되는 자당 대체물이다. 본 논문은 구강 중요 세균인 Streptococcus mutans, Streptococcus oralis, Streptococcus salivarius에서 유당분해에 대한 자일리톨의 작용을 관찰하고자 하였다. 세균에 대한 유당과 자일리톨의 병합효과를 보기 위하여 분광광도계를 이용한 흡광도를 측정하였고 생균수 검사를 실시하였다. Streptococcus mutans 또는 β-galactosidase에 의한 유당분해에 대한 자일리톨 효과를 보기 위하여 thin layer chromatography와 lactose-PTS activity test를 실시하여 다음과 같은 결과를 얻었다. 1. 유당과 자일리톨을 병합 첨가한 배지에 Streptococcus mutans를 배양하면 배양액 흡광도가 배양 8시간에 증가하지 않다가 배양 24시간에 증가하였다. 배양 8시간에서의 생균수도 유당 단독보다 유당과 자일리톨 병합 첨가 시 적게 나타났다. 2. 유당과 자일리톨을 병합 첨가한 배지에 Streptococcus oralis, Streptococcus salivarius를 배양하면 배양액 흡광도가 배양 8시간에 증가하지 않다가 배양 24시간에 증가하였다. 3. 배지에 유당 단독으로 첨가할 때보다도 유당과 자일리톨을 병합 첨가한 배지에 Streptococcus mutans를 8시간 배양하면 thin layer chromatography상 남아있는 유당이 많았으나, 배양 24시간에는 모든 유당이 분해되었다. 4. 배지에 유당 단독으로 첨가할 때보다도 유당과 자일리톨을 병합 첨가한 배지에서 Streptococcus mutans의 lactose-PTS 활성도가 낮았다. 5. 배지에 유당 단독으로 첨가할 때보다도 유당과 자일리톨을 병합 첨가한 배지에 β-galactosidase 사용시 thin layer chromatography상 유당이 많았다. 이상의 결과는 자일리톨이 Streptococcus의 유당 이용을 억제함을 시사하였다. Xylitol is a 5-carbons carbohydrate, which can be replaced with sucrose for preventing dental caries. To study the effect of xylitol on the fermentation of lactose in bacteria, the important oral bacteria such as Streptococcus (S.) mutans, S. oralis and S. salivarius were studied. The optical density using spectophotometer and the cell concentration were assessed to evaluate the combined effect of lactose and xylitol against the bacteria. Thin layer chromatography and lactose-PTS activity test were performed to evaluate the effect of xylitol on the fermentation of lactose in S. mutans and by β-galactosidase with the following results. 1. The optical density of Streptococcus mutans culture was not increased for 8 hours-incubation in the media added with lactose and xylitol, but was increased at 24 hours-incubation. The number of viable cells at 8 hours-incubation was smaller in the media containing lactose and xylitol in comparison with lactose only. 2. The optical densities of Streptococcus oralis culture and Streptococcus salivarius culture were not increased for 8 hours-incubation in the media added with lactose and xylitol, but were increased at 24 hours-incubation. 3. When Streptococcus mutans was incubated for 8 hours in the media added with lactose and xylitol. the amount of remained lactose was larger compared with the media added with lactose only. But all lactose was fermented in both media after 24 hours-incubation. 4. When Streptococcus mutans was incubated in the media added with lactose and xylitol, the activity of lactose-PTS was higher compared with the media added with lactose only. 5. When β-galactosidase was incubated in the media added with lactose and xylitol, the amount of remained lactose was larger compared with the media added with lactose only. These results indicated that xylitol inhibited the fermentation of lactose by Streptococcus.