Stain improvement in the white button mushroom ‘Seolgang’ and its varietal characteristics in Agaricus bisporus

Byung-Joo Lee,Mi-Ae Lee,Yong-Gyun Kim,Kwang-Won Lee,Yong-Pyo Lim,Byung-Eui Lee,Ho-Yeon Song 한국버섯학회 2012 한국버섯학회지 Vol.10 No.4

The button mushroom (Agaricus bisporus) is one of the most widely cultivated important edible mushroom species. In the breeding of new button mushroom, ‘Seolgang’ was developed by crossing two monokaryons ‘CM020913-27’ and ‘SSU423-31’. Because of the secondarily homothallism, only a small percentage of the basidia produce 3 or 4 spores, which are mostly haploid (n) and do not fruit. Single spore cultures derived from these types of spores produce a vegetative mycelium that also contain a variable number of genetically identical nuclei per cell called monokaryon. The lack of clamp connections between monokaryon and dikaryon required a series of mycelial culture and fruiting test. After crossing, hybrids were cultivated on a small scale and on a commercial scale at a farm. For this, the spawn was made by a commercial spawn producer and the spawned compost by a commercial compost producer. Mycelial growth of ‘Seolgang’ on CDA was better at 20℃ and 25℃ when it was compared with that of ‘505 Ho’. The mature cap shape of new strain ‘Seolgang’ is oblate spheroid and the immature cap shape is round to oblate spheroid. The cap diameter was 41.2 mm on average. In comparison with white strain ‘505 Ho’, the strain had a yield that was 9% higher. It produced fruiting bodies which had a higher weight on average per fruiting body and were 19% firmer with a good shelf life. Days of fruiting body were 3-4 days later than those of ‘505 Ho’. The physical characteristics such as elasticity, chewiness, adhesiveness were better than that of ‘505 Ho’. Genetic analysis of the new strain ‘Seolgang’ showed different profiles compared to ‘505 Ho’, CM02913-27, SSU413-31, when RAPD primers A02 and O04 were used.

Vibrio cholerae균 분리를 위한 새로운 분별배지

송호연,고광균 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

The purpose of this experiment is to develop a new differential medium for Vibrio cholera. The TCBS agar medium is used commonly for the identification of vibrio species, but it contains some inhibitors against other bacteria to increase the selectivity for vibrio species. So we developed the BYS agar medium as a new differential medium for V. cholera. We compared the colony forming unit(CFU) between BYS and TCBS using streak culture method and micro-pour plate method(100 ㎕). The CFU count was more than thirty times higher in BYS agar medium than in TCBS in both methods. These results suggest that BYS agar medium is a differential medium better than TCBS agar medium for the identification of V. cholera.

사람의 혈관 내피세포에 대한 Salmonella 균종의 감염

송호연,고광균,최순룡 순천향대학교 1994 논문집 Vol.17 No.4

In order to elucidate the mechanism of injury to endothelial cell of blood vessels in typhoid fever, Salmonella typhi was inoculated in cultured human endothelial cells. Endothelial cells were prepared from the umbilical vein that was freshly obtained from human umbilical cord by collagenase treatment. After screening of cultured endothelial cells through the immunofluorescent antibody technique, ultrastructural change of cytopathic Aeffect, obtained by lectron microscopy, showed typical progress of pathogenesis caused by S. typhi; adherence, endocytosis, intracellular replication and release of microorganism. These patholgical changes led to cellular disruption and final death of endothelial cells. Endothelial cells of vascular system found to be the site of amplification as well as the target organ of S. typhi, which could play important role in hematogenous spread of this microorganism and also in pathologic sign or symptom in typhoid fever. An experimental infection of S. typhi in cultured human endothelial cells found to be an excellent and valuable virulence assay system. The above ability of S. typhi to proliferate and lead to endothelial cell death in cultured human endothelial cells confirmed to be a factor of endovascular disorder of typhoid fever such as rose spot on abdomen of patients.

결핵균 TB-14 유전자의 클로닝

송호연,고광균 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The purpose of this study is to find a Mycobacterium tuberculosis specific protein for a new diagnositic method of tuberculosis. We found a protein reacting with Kris antiserum(goat antiserum against Mycobacterium intracellulare) in culture filtrate of Mycobacterium avium LR114F by Western blot. The N-terminal amino acid sequence of this if compared with that of Mycobacterium tuberculosis using TIGR Database. It is cloned, expressed in E. coli. Recombinant TB-14 protein of M. tuberculosis is composed of 148 amino acid including 30 amino acid of signal peptide and it showed 78% of homology with M. avium. These results suggest that recombinant TB-14 protein may be useful for the devolpment of new diagnostic method for tuberculosis.

RF-magnetron 스퍼터링에 의해 제조된 HAp와 HAp-Ag 복합재료의 미세구조와 In-vitro 연구

송호연,이희정,고광균 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Microstructure and in-vistro study of RF-magnetron sputtered HAp and HAp-Ag composite films on the ZrO_(2) substrate were investigated using XPS, SEM, AFM and HR-TEM techniques. The homogenous and dense films about 0.8 ㎛ in thickness were successfully obtained and they were comprised with nano-crystalline grains about 30 nm in diameter. Micro cracks were not found at the interfaces between HAp and ZrO_(2) substrate and also on theire films. Without the dependency of Ag addition the fracure mode of HAp-Ag films showed a transgranular type. Due to the excellent bioaffinity, osteoblast MG-63 cells wre grown well on the HAp and HAp-Ag films with dense and healthly morphology, although the growth rate of cells on the HAp-Ag film was slower than that of the HAp film.

연속 다공질 생체세라믹에 대한 생체친화성 연구

송호연,고광균 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Continuously Al_(2)O_(3) porous bodies were fabricated by multi-extrusion process. Their biocompatibility was evaluated through in vitro and in-vivo study. Behavior of osteoblast adhesion and proliferation grown on Al_(2)O_(3) porous body was observed. Human osteoblast-like MG-63 cells were grown well on top, bottom, and outside as well as inside surface of porous body. Al_(2)O_(3) the ideal pore size was 150-200 um. The 3- dimensional continuously porous Al_(2)O_(3) body was implanted in subcutaneous tissue of rat for in-vivo study. The porous body was covered with well-developed fibrous tissues and showed the formation of new capillaries in it. No inflammatory response around porous body was found. From the results, the bio-ceramic materials used in the research was proved to have excellent biocompatibility.

연구소 직무등급시스템의 개발과 활용 (JFSL 모형) : 인도네시아 과학기술평가청 사례

송계충,조영호,이광순 충남대학교 경상대학부설 경영경제연구소 1996 경영논집 Vol.12 No.2

인도네시아 연구기관의 인적자원관리 시스템 개발을 위한 목표모형

송계충,조영호,이광순 충남대학교 경상대학부설 경영경제연구소 1996 경영논집 Vol.12 No.2

그람음성 세균에 대한 나노실버의 항균효과

송호연,고광균 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Silver is one of the most universal anti-microbial substances. To improve the anti-microbial activity of silver, we successfully produced nanosilver by nano-technology. We examined the anti-microbial activity of nanosilver for several Gram negative pathogenic bacteria(Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi). Most bacteria were controlled within 1 ppm of nanosilver witin 1 hour. The mechanism of anti-microbial effects was explored by SEM and TEM. The severe morphologic changes, irregular cell wall, increased electron density of cytoplasm, and plasmolysis, were observed. From the result, nanosilver will be available as an antibiotics alternative.

흰쥐에서 음경발기 평가의 척도로서 음경해면체내압측정술 확립

송윤섭,김용준,이광우,김준모,문기혁,박영호,민영기,유형균,김형건 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Purpose: Because studies about the control of penile erection at the entral nervous system was dependent on the animal experiments, establishment of measuring penile erection is important to evaluate the effects changes at the central nervous system on the penile erection. Intracavernous pressure measurement in rats has been introduced as a suitable index for the evaluation of penile erection but this method is invasive and technically difficult. So, we established the intracavernous pressure measurement in rats as the experimental index for penile erection. Materials and Methods: 42 male adult Sprague-Dawley rats (250-300gm) were divided to saline or papaverine treated group. Rats were placed on a heating table to maintain their body temparature and anesthetized with 50mg/kg of pentobarbital sodium by intraperitoneal injection. A cannula was inserted to the left carotid artery to measure systemic blood pressure. Saline (0.05, 0.1ml), papaverine(0.05, 0.1, 0.25, 0.5, 1.0, 2.0mg) were injected intracavernously via 26 guage needle filled with saline or papaverine which connected to pressure transducer and polygraph was inserted into the corpus cavernosum on one side to inject the drug and to measure intracavernous presure. Results: Properly executed insertion and intracavernous administration produced a instantaneous but transient rise in intracavernous pressure that substantially stabilized and maintained at 5.4±0.4mmHg. Intracavernous pressure and duration of penile blood flow following intracavernous injection of papaverine are increased comared to those of saline. Conclusions: Monitoring intracavernous pressure in rats represents a suitable index for the evaluation of penile erection in small laboratory animals.