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Glass Ionomer 수복재와 와동이장재의 항균성에 관한 연구
음종혁,김신 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.1
Microorganisms may exist at the interface between prepared cavity and restorative material due to microleakage or incomplete removal of carious lesion, and this could result in pulpitis or secondary caries. In this perspective, the antibacterial potential of the restorative materials and cavity liners would be advantageous. The antibacterial effect of glass ionomer cements has already been proven but only partially, and there has not been much of a general comparative evaluation of the antibacterial effect of the materials being provided in an increasing number in these days. This research was intended to compare the antibacterial activity of glass ionomer restorative materials and glass ionomer cavity liners and resin cavity liner by employing agar diffusion test. The antibacterial activity of the Glass inomer materials including conventional chemically-cured glass ionomer cement, glass cermet cement, and light-cured glass ionomer cement to Streptococcus mutans, a leading causative microorganism in caries, was evaluated, which yielded the results as follows. 1. All the glass ionomer materials tested proved to have antimicrobial effects. 2. In the case of glass ionomer restorative materials, the bacterial inhibition zone was largest in the Vitremer and smallest in Fuji Ⅱ LC. Ketac-Silver and Ketac-Fil exhibited similar values (P<0.05). 3. In the case of glass ionomer cavity liners, it was in the order of Vitrebond, Fuji Lining Cement, Fuji Lining LC, with the latter showing the smallest zone (P<0.05). On the contrary, Timeline which is a kind of F-added resin cavity liners showed no antibacterial activity. 4. No material showed antibacterial activity when applied after immersed in saline for 24 hrs. 5. It was impossible to clarify the difference in antibacterial effect between light-cured and chemically-cured GIC materials and GIC cavity liners exhibited greater antibacterial effects comparing with GIC restorative materials (P<0.05).
Genome Analysis of the Domestic Dog (Korean Jindo) by Massively Parallel Sequencing
Kim, Ryong Nam,Kim, Dae-Soo,Choi, Sang-Haeng,Yoon, Byoung-Ha,Kang, Aram,Nam, Seong-Hyeuk,Kim, Dong-Wook,Kim, Jong-Joo,Ha, Ji-Hong,Toyoda, Atsushi,Fujiyama, Asao,Kim, Aeri,Kim, Min-Young,Park, Kun-Hyan Oxford University Press 2012 DNA research Vol.19 No.3
<P>Although pioneering sequencing projects have shed light on the boxer and poodle genomes, a number of challenges need to be met before the sequencing and annotation of the dog genome can be considered complete. Here, we present the DNA sequence of the Jindo dog genome, sequenced to 45-fold average coverage using Illumina massively parallel sequencing technology. A comparison of the sequence to the reference boxer genome led to the identification of 4 675 437 single nucleotide polymorphisms (SNPs, including 3 346 058 novel SNPs), 71 642 indels and 8131 structural variations. Of these, 339 non-synonymous SNPs and 3 indels are located within coding sequences (CDS). In particular, 3 non-synonymous SNPs and a 26-bp deletion occur in the <I>TCOF1</I> locus, implying that the difference observed in cranial facial morphology between Jindo and boxer dogs might be influenced by those variations. Through the annotation of the Jindo olfactory receptor gene family, we found 2 unique olfactory receptor genes and 236 olfactory receptor genes harbouring non-synonymous homozygous SNPs that are likely to affect smelling capability. In addition, we determined the DNA sequence of the Jindo dog mitochondrial genome and identified Jindo dog-specific mtDNA genotypes. This Jindo genome data upgrade our understanding of dog genomic architecture and will be a very valuable resource for investigating not only dog genetics and genomics but also human and dog disease genetics and comparative genomics.</P>
Detection of Helicobacter pylori in Gastric Aspirates Using a Monoclonal Antibody-Based Test
( Ho Dong Kim ),( Do Hyun Kim ),( Hyeuk Park ),( Woo Jong Kim ),( Yong Soo Ahn ),( Young Jik Lee ),( Sun Mi Park ),( Eun Seon Seo ),( Chul Park ),( Yang Ho Kim ),( Hyung Rag Kim ),( Young Eun Joo ),( 대한소화기학회 2013 Gut and Liver Vol.7 No.1
Background/Aims: The objective of this study was to evaluate a monoclonal antibody-based test to detect Helicobacter pylori-specific antigen in gastric aspirates from humans. Methods: Sixty-one volunteers were enrolled in the study. All of the subjects underwent a 13C-urea breath test (UBT) before esophagogastroduodenoscopy. Gastric aspirates were analyzed for pH and ammonia and used for polymerase chain reaction (PCR), culture, and monoclonal antibodybased detection of H. pylori. Multiple biopsies of the gastric antrum and body were obtained for a rapid urease test (RUT) and histological evaluation. Results: Thirty-six subjects were H. pylori-positive and 25 were H. pylori-negative according to the UBT results. Compared with the H. pylori-negative subjects, H. pylori-positive subjects had a higher pH (4.77±1.77 vs 3.49±1.30, p<0.05) and ammonia level (1,130.9±767.4 vs 184.2±126.3, p<0.0001). The sensitivities and specificities of the PCR test, RUT, culture test, and monoclonal antibody-based test were 100% and 72%, 89% and 100%, 47% and 100%, and 78% and 100%, respectively. Conclusions: The monoclonal antibody-based test for diagnosing H. pylori infection in gastric aspirates has increased sensitivity compared with the culture test and specificity as high as that of the RUT. The test may be useful as an additive test for examining gastric aspirates. (Gut Liver 2013;7:30-34)
New monoclonal antibody-based test for Helicobacter pylori urease in gastric tissue
( Do Hyun Kim ),( Ho Dong Kim ),( Hyeuk Park ),( Seung Choi ),( Jae Won Beom ),( Woo Jong Kim ),( Chang Kook Park ),( Young Jik Lee ),( Ju Young Park ),( Hyung Rag Kim ),( Chul Park ),( Young Eun Joo 대한내과학회 2016 The Korean Journal of Internal Medicine Vol.31 No.1
Background/Aims: To evaluate a new monoclonal antibody for Helicobacter pylori urease in gastric tissue. Methods: A total of 107 volunteers were enrolled. All subjects underwent a 13C-urea breath test and esophagogastroduodenoscopy. Gastric aspirates were analyzed for pH and ammonia. Six biopsy specimens in the gastric antrum and body were obtained for a rapid urease test and histology. The new monoclonal antibody-based H. pylori urease test (HPU) was performed to rapidly and qualitatively detect urease in two biopsy specimens. Results: H. pylori infection was diagnosed in 73 subjects. The sensitivity and specificity of the HPU was 89% and 74%, respectively. The subjects were divided into two groups: one with true-positive and true-negative HPU results (n = 90) and the other with false-positive and false-negative HPU results (n = 17). Across all subjects, ammonia levels were 900.5 ± 646.7 and 604.3 ± 594.3 μmol/L (p > 0.05), and pH was 3.37 ± 1.64 and 2.82 ± 1.51 (p > 0.05). Sensitivity was higher in the presence of atrophic gastritis or intestinal metaplasia. Conclusions: HPU detected H. pylori in approximately 10 min. Gastric aspirate ammonia and pH levels did not affect the test results. Sensitivity was good in the presence of atrophic gastritis or intestinal metaplasia.
김동한 ( Dong Han Kim ),박혁 ( Hyeuk Park ),문승원 ( Seung Won Moon ),정종혁 ( Jong Hyuk Jeong ),양혁승 ( Hyuk Seung Yang ),김도현 ( Do Hyun Kim ),김호동 ( Ho Dong Kim ) 대한소화기학회 2007 대한소화기학회지 Vol.50 No.2
Acute hepatitis E is an endemic disease, commonly reported in Indian subcontinent, China, Africa, Central America, and so forth. It is a self-limiting disease like other acute hepatitis except in pregnant patient. Although sporadic hepatitis E is noted all over the world, most of them are associated with travel history to HEV-endemic area. In Korea, Hepatitis E is rarely reported. Moreover, sporadic acute hepatitis E without travel history to HEV-endemic area is very rare. We experienced three sporadic cases of acute hepatitis E, without travel history. All of them presented acute hepatitis symptoms, elevated aminotransferase, and positive IgM HEV Ab. Symptoms and aminotransferase levels were normalized during hospitalization and IgM HEV Ab converted negative after 4-8 months. We report three sporadic cases of onset-acute hepatitis E without travel history to HEV-endemic area.(Korean J Gastroenterol 2007;50:121-125)