The expression patterns of RANKL and OPG in murine tooth eruption

Hwang, Kyung-Mun,Kim, Eun-Jung,Kim, Hyun-Jung,,Kim, Young-Jin,Nam, Soon-Hyeun 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

치아의 맹출은 치아기 (dental organ)와 치조골의 세포와 연관된 매우 복잡한 과정이다. 우선 치아 맹출이 일어나기 전에 파골세포가 치낭으로 집결하게 된다. 이러한 치낭의 역할은 파골세포와 조골세포의 상호작용으로 이루어 지는 골개조와 밀접한 관련이 있는데, 이는 치아 맹출과 연관된 많은 유전자들이 치낭에서 발현되기 때문이다. RANKL는 TNF ligand family로써 조골세포에 존재하며 파골세포의 형성 및 전구세포로 부터의 활성화를 유도한다. 이러한 RANKL은 OPG에 의해 그 작용이 억제되며 RANKL와 OPG의 상대적인 비율이 파골세포의 형성에 영향을 미친다. 또한 Runx2유전자의 변이는 조골세포의 분화와 활성에 차질을 가져오고 결국 RANKL/OPG pathway를 통해 파골세포 형성에 영향을 줄 수 있다. 치아의 발육 및 맹출에 미치는 RANKL 및 OPG의 영향을 알아보고 Runx2와의 연관성을 알아보기 위해 in situ hybridization 방법으로 태생 1, 3, 5, 7, 9, 11일된 쥐의 하악 및 제1대구치를 사용하여 실험을 실시한 결과 RANKL, OPG, Runx2의 mRNA가 태생 1일부터 11일까지 치낭 및 치아주위조직에 특성있게 나타났다. 이중 태생 5일에서 9일 사이에 RANKL 및 Runx2는 치아의 교합면측과 하방 치조골 부위의 발현이 강하게 나타난 반면 OPG는 약한 발현을 보였다. 이는 또한 파골세포의 활성부위를 알아보기 위해 TRAP염색을 실시하여 태생 5일에서 9일 사이에 최대의 활성화를 나타낸 결과와 연광성 있게 나타났다. RANKL, OPG, Runx2의 특성있는 발현양상들을 종합해 볼 때, 치아 맹출은 치낭, 치아기, 치조골 사이의 상호 작용을 통해 이루어 지며, 이는 치낭이 치아 맹출에 있어서 매우 중요하다는 것을 의미한다. 또한, 이러한 유전자들 (RANKL, OPG, Runx2) 이 치아의 맹출에 중요한 역할을 하는 것으로 사료된다. Tooth eruption is a complex and tightly regulated process that involves cells of the tooth organ and the surrounding alveolus. Osteoclast precursors must be recruited into the dental follicle prior to the onset of eruption. This function of dental follicle may be regarded as the ability of bone remodeling characterized by the interaction of osteoclasts and osteoblasts. This is because tooth eruption is a localized event in which many of the genes required for eruption are expressed in the dental follicle. RANKL is a membrane-bound protein that is a member of the TNF ligand family, which is present on bone marrow stromal cells and osteoblasts, and induces osteoclast formation and activation from precursor cell. The biologic effect of RANKL is inhibited by OPG and, in bone, the relative ratio of RANKL and OPG modulates osteoclastogenesis. To evaluate the roles of RANKL and OPG in tooth eruption and the relations with the expression pattern of Runx2, in situ hybridization was performed with mandibles of mice at postnatal stage 1, 3, 5, 7, 9 and 11. mRNA of RANKL, OPG, and Runx2 are expressed in dental follicle and surrounding tissue from P1 to 11. To determine the sites of osteoclastic activity duing tooth eruption, mandibles were dissected. Peak osteoclastic activity in alveolar bone along the occlusal and basal regions was observed from P5 to 9, with osteoclasts in these regions being large and strongly TRAP-positive. The specific spatic-temporal expression patterns of RANKL, OPG and Runx2 in our study suggest that tooth eruption could be progressed through the interactions of molecular signaling among dental follicle, dental organ and alveolar bone, furthermore it means that dental follicle is quite important in tooth eruption. In addition, it indicates that these genes (RANKL, OPG, and Runx2) play critical roles in tooth eruption.

과학적 근거 중심 평가를 도입한 마늘의 콜레스테롤 조절 기능성 평가

김인혜;조강진;김진영;황경아;황유진;채유진;엄애선 한양대학교 2010 韓國 生活 科學 硏究 Vol.30 No.1

Garlic (Allium sativum) is used a flavouring agent and a traditional medicine in human history. Numerous studies support the fact that garlic intake may have effect on cholesetrol-lowering. No claims currently have been made about garlic intake and cholesterol-lowering effect with respect to health claim in Korea. The aim of this study was to evaluate the scientific evidence on garlic intake and cholesterol-lowering effect by the scientific evidence-based rating system of Korean Food and Drug Administration for health claim. We carried out a comprehensive search of the Pubmed, EMBASE, Cochrane Library and manual searches of relevant journals for studies between 1955-2009. The search terms were garlic and Allium sativum in combination with cholesterol. Eighteen clinical stidies were included and reviewed to evaluate the strength of evidence that supports a relation between garlic intake and cholesterol-lowering effect. Our results suggests that level of scientific evidence on garlic intake and cholesterol-lowering effect is "other function (II) probable".

5-〔3-(N-Substituted Piperazinyl)Propoxy〕Indan類의 合成과 中樞神經抑制效果

黃熙喆,閔庚鎭,文勝煜,李萬佶,徐丙天 嶺南大學校附設 基礎科學硏究所 1985 基礎科學硏究 Vol.5 No.-

Five new indans, 5-[3-(N-substitued piperaziny) propxy]indans, were synthesized by condensation of 5-(3-bromopropoxy)indan, the intermediate, and N-substituted piperazines. The structure of the compunds were determined by means of IR, NMR spectoscopy along with elemental analysis. Each compound was tested for antimorphine and antiamphetamine effects as part of CNS depressant effects and for muscle relaxant action in mice. Thes compound exhibited less potent activity compared to chlordiazepoxide on CNS depressant effect. Muscle reaxant action of the compounds, the side effect of tranquilizer, however, was much weaker than that of chlordiazepoxide.

리본 형태의 근관에서 열연화주입법의 근관벽에 대한 적합도에 관한 연구 : ADAPTATION TO CANAL WALLS

황현숙,조경모,김진우 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.4

The aim of this study is to compare the adaptability of thermoplasticized injectable gutta-percha technique to the canal walls in ribbon-shaped canals. Thirty resin models simulated ribbon-shape canals were instrumented to #40 using. 06 taper Profile systems. Three groups of each 10 resin models were obturated by the lateral condensation technique(LC) and the two thermoplasticized injectable gutta-percha technique; Ultrafil Endoset+Obtura Ⅱ(EO) and Ultrafil Firmset(UF), respectively. After resin model were kept at room temperature for 4 days, they were resected horizontally with microtome at 1, 2, 3, 4 and 5mm levels from apex. At each levels, image of resected surface were taken using CCD camera under a stereomicroscope at ×40 magnification and stored. Ratio of the area of gutta-percha was obtained by calculating area of gutta-percha cone to the total area of canal using digitized image-analyzing program. The data were collected then analyzed statistically using One-way ANOVA. The results were as follows. 1. At 1mm levels, there was no statistically significant difference in the mean ratio gutta-percha among the groups. 2. At 2mm level, EO showed the highest mean ratio of gutta-percha (p<0.05) and there was no significant difference between LC and UF. 3. At 3, 4, 5mm levels, EO and UF and had significantly greater mean ratio of gutta-percha than LC(p<0.05) and there was no significant difference between EO and UF. In conclusion, the thermoplasticized injectable gutta-percha techniques demonstrated relatively favorable adaptability to canal walls than lateral condensation technique in ribbon-shaped canals except for 1mm level.

有用竹類의 利用開發을 爲한 特性調査 : 觀賞을 中心으로

黃震聲,金是璟 진주산업대학교 1970 論文集 Vol.5 No.-

우리나라 全體에 分布되어 있는 15種의 竹類와 標本園에 심어져 있는 40여종의 外來産에 對하여 觀賞을 中心으로 그 特性을 調査하여 얻어진 結果를 要約하면 다음과 같다. 1. 樹相全體에 觀賞價値를 가져 庭園에 심어질 수 있는 것이 16種. 2. 竹稈에 觀賞價値를 가진 것이 8種. 3. 잎에 그 價値를 가진 것이 7種. 4. 土砂의 流出防止를 할 수 있는 것이 8種. 5. 盆栽用으로 適當한 것이 17種. 6. 여러 가지를 겸할 수 있는 것이 36種. 7. 竹皮가 빨리 떨어지는 것이 11種. 8. 언제나 竹皮가 稈에 붙어 있는 것이 12種. 9. 地下莖이 없이 株立되는 것이 3種이나 되었다. The results I have obtained by examining the characteristics, concentrating ornamental purpose of the fifteen kinds of bamboos throughout the country, and more than fourty kinds of foreign ones growing in some sample plots in this province, are summarized as follows: 1) The kinds of bamboos worth planting in gardens are 16 2) The kinds of bamboos with ornamental values in their canes are 8. 3) The kinds with ornamental values in their leaves are 17. 4) The kinds which are useful for soil loss prevention are 8. 5) The number of The kinds fit for pot plantation is 17. 6) The number of the kinds suitable for various purpose is 36. 7) The kinds which lose sheaths early while growing are 11. 8) The kinds which wear sheaths through their lives are 12. 9) The kinds which grow directly from their mother bamboo roots ( without subterranean stems ) are 3.

무용교수도구로서의 심상연구

황규자,배진경 용인대학교 학생생활연구소 1997 학생생활연구 Vol.5 No.-

In the various fields of physical education and dance education, Image Trainning, though it is not systematically organized, has been carried out by showing an example or using languages. This paper is primarily concerned with to what extent Image Trainning can fulfills its function in motor learning. The result is that not only does Image Trainning help develop motor learning, but students with higher imagery ability exceed those without the ability in learning. Accordingly, it is suggested in this paper that (1) teachers should regard Image Trainning as a supporting program in motor learning program, (2) dance teachers should make best use of the Image Trainning in classes to produce satisfactory results, (3) teachers should try to their best to develop imagery programs by studying a variety of parameters such as the characteristics of classes, practice span, student's ability and so on.

항만물류 XML/EDI 시스템의 개발

황성진,안선하,박경환 동아대학교 정보기술연구소 2002 情報技術硏究所論文誌 Vol.9 No.2

This thesis investigate Disadvantages of traditional Port Logistics EDI, and introduce how to design and implement XML/EDI system on based Microsoft's Biztalk Framwork in order to solve it. The foundational structure of this system constructed application and Biztalk server. Also flow of data is following: Datas input through application and store to XML-document. The next step transmit XML-document to BizTalk server. BizTalk server work mapping XML-document to standard EDI-document. And standard EDI-document transmit to custom house.

英國과 獨逸에 있어서의 産學協同에 關한 硏究

陳世仁,黃七星,禹洪九,尹慶宇,朴東熙 건국대학교 1974 學術誌 Vol.17 No.1

정형외과 영역에서 Zenol^(�)의 임상적 효과 판정 및 만성질환에서 Hot 및 Coll type의 비교

황병연,박명식,이준모,송경진,이강욱,허달영 최신의학사 1993 最新醫學 Vol.36 No.2

대학 무용전공 학생의 신체적·정신적 건강상태에 관한 연구

황규자,배진경 龍仁大學校 1998 용인대학교 논문집 Vol.16 No.-

Through the appeals of the subjective symptoms of university students specializing in dancing, to find out the influences that performing dances has on the individual health, I examined the differences of subjective symptoms with those in common students and understood what influences the careers of majoring in dancing has. By this I came to these conclusions through comparing and analyzing the scores of the appeals of subjective symptoms First, on the whole, the groups of students specializing in dancing have shown the lower average in distribution of the scores of the appeals of subjective symptoms than the groups of common students, especially the troubles in digestive organs, aggressiveness, impulsiveness, nervousness, and melancholia. Second, In comparing subjective symptoms in men and women has significantly shown the lower average in all clauses than women except for misrepresentation and the troubles in respiratory organs. Third, in comparing subjective symptoms of students majoring in dancing by the careers, the clauses of emotional unrest, melancholia and misrepresentation has shown significant differences. The longer career, the lower emotional unrest and melancholia, the longer career, the higher misrepresentation.