Description of a new species of the whitefly genus Pealius Quaintance & Baker (Hemiptera: Aleyrodidae) from China

Ji‐Rui Wang,Yu-Zhou Du,Jon H. MARTIN,Zhi-Hong XU 한국곤충학회 2018 Entomological Research Vol.48 No.5

A new whitefly species, Pealius yunnanensis sp. nov., collected from Mengzi, Caoba village (Yunnan, China) on Ficus microcarpa Linn. f. (Urticales: Moraceae), is described with morphology, line illustrations, photographs and scanning electron microscope images. In addition, a checklist of Chinese species of Pealius is provided and all Pealius species recorded from Ficus around the world are listed. Specimens have been deposited in the Insect Collection of Zhejiang Agriculture & Forestry University (ZAFU), Lin'an, China.

古代漢語에 나타난 色彩語 命名 模式에 대한 고찰

王繼紅 한국한문교육학회 2004 한문교육논집 Vol.23 No.-

본문은 『說文解字』, 『說文解字注』를 주요 자료로 삼아, 『爾雅』, 『釋名』등의 고대 중요 자서(字書)를 함께 이용하여 색채어의 의미변화와 그 단어 결합의 특징, 색의 정도에 따른 색의 표현 방법, 고대 사람들의 색에 대한 관념 등 여러 각도에서 논의를 시작하여 고대한어에 나타난 색을 표현하는 단어들의 命名模式에 대해 알아보고자 한다. 이를 위해, 인지심리학에서 연구되는 이론들을 이용하여 색채어의 발생과 발전을 여러 실제 상황을 통해 해석하고자 한다. 고대한어에 나타난 색채어의 命名模式은 크게 1) 借代命名模式, 2) 組合命名模式, 3) 比擬命名模式 4) 比較命名模式 등 네 가지로 분류할 수 있다. 本文以『說文解字』·『說文解字注』爲主要語料, 兼及『爾雅』·『釋名』等古代重要字書, 從顔色詞語的詞義演變與結구特征·顔色詞語的程度表示法, 以及先民的顔色觀念等궤個角度出發, 구擬古漢語顔色詞語的命名模式. 幷且運用認知心理學的有關理論對顔色詞語産生和發展的某些事實做出解釋. 古漢語顔色詞語命名模式主要包括: 一. 借代命名模式 ; 二. 組合命名模式 ; 三. 比擬命名模式 ; 四. 比較命名模式.

제조합 대장균에서 과발현된 Citrobacter freundii KCTC2006 유래의 β-Tyrosinase를 이용한 3,4-Dihydroxyphenyl-L-alanine의 생산

이승구,노현수,홍승표,이규종,왕지원,태동년,엄기남,방상구,김영준,성문희 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

재조합 대장균에서 대량발현 시킨 Citrobacter freundii KCTC 2006 유래이 효소 β-tyrosinase를 이용하여 pyrocatechol, sodium pyruvate, ammonium acetate로부터 3,4-dihydroxy phenyl-L-alanine을 생산하기 위한 연구를 수행하였다. 이 효소반응에 적합한 온도 및 pH 조건은 각각 18℃와 8.5로 결정되었고, 반응액 중의 ammonium acetate와 sodium pyruvate의 농도는 각각 300 mM, 50 mM 이상으로 조절하는 것이 적합하였다. Pyrocatechol의 경우는 20 mM에서 가장 높은 반응성을 나타냈으나, 기질을 반복적으로 첨가하며 장시간 동안 효소반응을 수행하는 경우에는 pyrocatechol의 고갈을 피하기 위하여, 20 mM에서 50 mM 사이로 조절하였다. 한편, 반응액 중에 ethanol을 10% 첨가한 경우에는 반응속도가약 20% 증가하였다. 이상과 같은 효소반응특성에 기초하여 조제한 기질용액에 β-tyrosinase를 1 unit/㎖ 농도로 가하고, pyrocatechol과 pyruvate가 고갈되지 않도록 간헐적으로 첨가하면서 효소반응을 수행한 결과, 24시간 만에 85.2%의 수율로 31.6g/ℓ의 3,4-dihydroxyphenyl-L-alanine를 생산할 수 있었다. By using the β-tyrosinase of Citrobacter freundii KCTC2006, which was cloned and overexpressed in Escherichia coli, 3,4-dihydroxy phenyl-L-alanine (L-DOPA) was synthesized efficiently from pyrocatechol, sodium pyruvate, and ammonium acetate. Optimal temperature and pH for the reaction were determined to be about 18℃ and 8.5, respectively. The effects of substrate concentrations were also examined at different concentrations of ammonium acetate, sodium pyruvate, and pyrocatechol. Ammonium acetate and sodium pyruvate increased the reaction rate until the concentrations reached to 300 mM and 50 mM, respectively. Although pyrocatechol showed the optimal concentration at 20 mM, it was controlled between 20 mM and 50 mM to avoid the depletion of substrate during the enzymatic synthesis. Based on above results, a reaction medium for the production of L-DOPA was prepared and incubated with 1 unit/㎖ of β-tyrosinase. Pyrocatechol and sodium pyruvate was added to the reaction solution intermittently to avoid the substrate depletion during the enzymatic reaction. After 24 hour of reaction, 31.6 g/ℓ L-DOPA was accumulated in the reaction solution as soluble and precipitated ones and the conversion yield was about 85.2%.

Study on a Method of Node Selection in Fuzzy Polynomial Neural Networks

Ji-Hong Wang(王繼紅),Tae-Chon Ahn(안태천),Sung-Kwun Oh(오성권) 대한전기학회 2017 대한전기학회 학술대회 논문집 Vol.2017 No.7

Transiting Exoplanet Monitoring Project (TEMP). IV. Refined System Parameters, Transit Timing Variations, and Orbital Stability of the Transiting Planetary System HAT-P-25

Wang, Xian-Yu,Wang, Songhu,Hinse, Tobias C.,Li, Kai,Wang, Yong-Hao,Laughlin, Gregory,Liu, Hui-Gen,Zhang, Hui,Wu, Zhen-Yu,Zhou, Xu,Zhou, Ji-Lin,Hu, Shao-Ming,Wu, Dong-Hong,Peng, Xi-Yan,Chen, Yuan-Yuan Astronomical Society of the Pacific 2018 Publications of the Astronomical Society of the Pa Vol.130 No.988

Selection of Reliable Reference Genes for Real-time qRT-PCR Analysis of Zi Geese (Anser anser domestica) Gene Expression

Ji, Hong,Wang, Jianfa,Liu, Juxiong,Guo, Jingru,Wang, Zhongwei,Zhang, Xu,Guo, Li,Yang, Huanmin Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.3

Zi geese (Anser anser domestica) belong to the white geese and are excellent layers with a superior feed-to-egg conversion ratio. Quantitative gene expression analysis, such as Real-time qRT-PCR, will provide a good understanding of ovarian function during egg-laying and consequently improve egg production. However, we still don't know what reference genes in geese, which show stable expression, should be used for such quantitative analysis. In order to reveal such reference genes, the stability of seven genes were tested in five tissues of Zi geese. Methodology/Principal Findings: The relative transcription levels of genes encoding hypoxanthine guanine phosphoribosyl transferase 1 (HPRT1), ${\beta}$-actin (ACTB), ${\beta}$-tubulin (TUB), glyceraldehyde-3-phosphate-dehydrogenase (GADPH), succinate dehydrogenase flavoprotein (SDH), 28S rRNA (28S) and 18S rRNA (18S) have been quantified in heart, liver, kidney, muscle and ovary in Zi geese respectively at different developmental stages (1 d, 2, 4, 6 and 8 months). The expression stability of these genes was analyzed using geNorm, NormFinder and BestKeeper software. Conclusions: The expression of 28S in heart, GAPDH in liver and ovary, ACTB in kidney and HPRT1 in muscle are the most stable genes as identified by the three different analysis methods. Thus, these genes are recommended for use as candidate reference genes to compare mRNA transcription in various developmental stages of geese.

Study on a Novel Learning Method for Fuzzy Polynomial Neural Networks

Ji-Hong Wang(王繼紅),Tae-Chon Ahn(안태천),Sung-Kwun Oh(오성권) 대한전기학회 2017 정보 및 제어 심포지엄 논문집 Vol.2017 No.4

B3GNT3 acts as a carcinogenic factor in endometrial cancer via facilitating cell growth, invasion and migration through regulating RhoA/RAC1 pathway‑associated markers

Ji-Shui Wang,Fang Ruan,Li-Zhu Guo,Feng-Ge Wang,Fu-Ling Wang,Hong-Min An 한국유전학회 2021 Genes & Genomics Vol.43 No.5

Background Aberrant expression of beta-1,3-N-acetylglucosaminyltransferase-3 (B3GNT3) has been frequently clarifed in various cancers, however, its role in endometrial cancer (EC) has not been assessed in detail. Purpose This study aimed to investigate the biological role of B3GNT3 in EC and simply explored the detailed mechanism. Methods The EC RNA-Seq dataset from TCGA database was applied to evaluate the expression of B3GNT3 and assess its role on prognostic value. HEC-1-A and KLE cell lines of EC were used to perform loss- and gain-of-function B3GNT3 assays respectively. Quantitative real-time PCR (qRT-PCR) and western blot were used to measure the mRNA and protein levels of indicated molecules respectively. Cell counting kit-8, clone formation tests, and Transwell assay served to determine the changes of proliferative, invasive and migratory abilities of EC cells after altering the expression of B3GNT3. Results B3GNT3 was found to be highly expressed in EC tissues compared to normal tissues according to the online public databases, which confrmed by the following qRT-PCR in 3 EC cell lines. Besides, high B3GNT3 expression presented a worse overall survival in EC patients as compared with low B3GNT3 expression group. Furthermore, functional experiments in vitro indicated that B3GNT3 could facilitate the cell growth, invasion and migration. Moreover, we found that downregulation of B3GNT3 signifcantly reduced the expression level of GTP-RhoA and GTP-RAC1, whereas upregulation of B3GNT3 presented the opposite results. Conclusion The results of current study demonstrate that B3GNT3 acts as an oncogene that promotes EC cells growth, invasion and migration possibly through regulating the RhoA/RAC1 signaling pathway-related markers, suggesting that B3GNT3 may be a candidate biomarker for EC therapeutic intervention.

Expression of Ang-2/Tie-2 and PI3K/AKT in Colorectal Cancer

Zhang, Ji-Hong,Wang, Li-Hua,Li, Xiang-Jun,Wang, Ai-Ping,Reng, Li-Qun,Xia, Feng-Guo,Yang, Zhi-Ping,Jiang, Jing,Wang, Xiao-Dan,Wen, Chun-Yang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Purpose: To study the expression of angiogenin-2 (Ang-2) and its receptor Tie-2 in colorectal cancer and discuss the possible mechanisms behind this process. Materials and Methods: Using the streptavidin-peroxidase (SP) immunohistochemical method, paraffin sections from 100 colorectal cancer samples and 10 samples from tumor-adjacent normal tissue (> 2 cm from the edge of the gross tumor) were tested for protein expression of Ang-2, Tie-2, PI3K, and AKT. Reverse transcription-polymerase chain reaction and Western blots were further used to measure expression of the 4 genes and proteins in 20 freshly-resected colorectal cancer samples and tumor-adjacent normal tissues. Results: In colorectal cancer tissues, the expression of the Ang-2, Tie-2, PI3K, and AKT genes and their proteins was significantly higher than in tumor-adjacent normal tissues. Protein expression in poorly-differentiated adenocarcinoma was higher than that in well and moderately differentiated adenocarcinoma. According to Duke's classification, the protein expression in Stages C and D was significantly higher than that in Stages A and B. In the group with lymphatic metastasis, the protein expression was higher than that without lymphatic metastasis. Conclusions: In colorectal cancer, the expression of the Ang-2, Tie-2, PI3K, and AKT genes and their proteins is markedly higher than those in tumor-adjacent normal tissues. No correlation was observed between protein expression and gender, location, or histologic type. Correlations did exist between protein expression and differentiation level, stage of Duke's classification, and lymphatic metastasis; in colorectal cancer tissues with lower differentiation levels, higher stages of Duke's classification, and lymphatic metastasis, the expression of all 4 proteins was higher. The study of their expression patterns and relationships with aggression and metastasis will provide a valuable experimental foundation for assessing prognosis and targeted therapy of colorectal cancer.

뉴캣슬병 바이러스 검출 및 병원성 감별을 위한 Duplex RT-PCR법 개발

김지예(Ji-Ye Kim),이현정(Hyun-Jeong Lee),장일(Il Jang),이희수(Hee-Soo Lee),윤성준(Seung-Jun Yoon),박지성(Ji-Sung Park),설재구(Jae-Goo Seol),김승환(Seung-Han Kim),홍지무(Ji-Mu Hong),Zillian Wang,Hualei Liu,최강석(Kang-Seuk Choi) 한국가금학회 2017 韓國家禽學會誌 Vol.44 No.2

본 연구에서 NDV의 L유전자와 F유전자를 표적 부위로 각각 제작한 primer 세트를 사용함으로써 하나의 PCR 튜브에서 NDV 검출(386 bp의 증폭 크기)과 함께 병원성 NDV (229 bp의 증폭 크기)를 동시에 감별 증폭할 수 있는 dRTPCR 검사법을 개발하였다. 개발된 dRT-PCR검사법은 NDV를 특이적으로 검출하고, 병원성을 감별하였다. 특히 국내 병성감정 실시기관에서 적용 중인 기존의 RT-PCR 상용키트에서는 검출하지 못하는 class I NDV과 PPMV(class II 유전형 VI형)을 NDV를 검출함과 동시에 병원성 NDV도 감별가능하였다. 개발된 dRT-PCR 검사법의 검출 민감도는 약 103.0EID50/0.1 mL로 평가되었다. 또한 ND발생국의 야외 시료에 적용했을 때, NDV 공통항원 검출율은 94.4%였으며, 병원성 NDV 검출율은 100%이었다. 그러므로 본 연구에서 개발한 dRT-PCR 검사법은 의심축 사례에서 ND를 신속 정확하게 진단하는 데 유용할 진단 방법을 제공할 수 있을 것으로 판단된다. A duplex RT-PCR (dRT-PCR) assay was developed for the simultaneous detection and discrimination of nonvirulent and virulent Newcastle disease virus (NDV) in a single PCR tube. Primers targeting the large polymerase protein (L) gene and the fusion protein (F) gene of NDV were designed to detect all NDVs (by common type PCR primers) and virulent NDVs (by pathotype PCR primers), respectively and evaluated experimentally with reference NDV strains and other poultry viral pathogens. PCR products of the expected size of 386 bp were amplified from all NDV samples whereas PCR products of the expected size of 229 bp were amplified from virulent NDV samples alone. Cross reaction was not observed with other avian viral pathogens. The detection limit of NDV by the dRT-PCR was estimated to be 103 50% egg infectious dose/0.1 mL. In the dRT-PCR using field isolates of NDV, the pathotype PCR primers detected specifically all of virulent field isolates of NDV from Malaysia, Pakistan and China whereas common type PCR primers detected 94.4% (51/54) of field isolates of NDV from China. Three Chinese NDV isolates with false negative result were non-virulent viruses. Our results indicate that the dRT-PCR might provide a rapid and simple tool for rapid simultaneous detection and discrimination of non-virulent and virulent NDVs. Therefore the developed dRT-PCR assay provides a powerful novel means for the rapid diagnosis of Newcastle disease.