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      • KCI등재

        족관절 삼과 골절에서 작은 후과 골편에 대한 나사못 고정과 비고정의 결과 비교

        고지욱 ( Jee-wook Ko ),이건우 ( Gun-woo Lee ),이근배 ( Keun-bae Lee ) 대한골절학회 2021 대한골절학회지 Vol.34 No.1

        목적: 족관절 삼과 골절에서 관절면의 25% 이내를 침범하는 작은 후과 골편에 대한 나사못 고정술 여부에 따른 결과를 비교하고자 하였다. 대상 및 방법: 후과 골편이 관절면의 15%-25%를 침범한 총 32예(32명)를 대상으로 내고정 여부에 따라 두 군(고정군 20예, 비고정군 12예)으로 분류하였으며, 최소 추시 기간은 12개월이었다. 고정군과 비고정군에서 후과 골편 크기의 중앙값은 각각 24.6% (범위 22.3%-25.0%)와 22.1% (범위17.4%-24.3%)였다. 두 군 간의 임상적 및 방사선적 결과를 비교 분석하였다. 결과: 최종 추시상, 두 군 간 AOFAS ( p=0.501), VAS (p=0.578), 족관절 운동 범위(p=0.552) 등의 임상적 결과의 차이는 없었다. 방사선적 분석 결과로 두 군 간 관절면 층 형성(p=0.289), 골편 간극(p=0.289)의 차이는 없었다. 합병증은 고정군에서 지연 유합이 1예, 창상 감염이 1예 있었다. 결론: 족관절 삼과 골절에서 작은 후과 골편에 대한 나사못 고정군과 비고정군은 모두 만족스러운 결과를 보여주었다. 따라서, 작은 후과 골편을 동반한 경우 관절면의 정복이 이루어진다면 추가적인 내고정술을 시행하지 않더라도 만족할만한 결과를 얻을 수 있을 것으로 생각한다. Purpose: This study was undertaken to compare outcomes of screw fixation and non-fixation of a small-sized posterior malleolar fragment involving less than 25% articular surface in ankle trimalleolar fractures. Materials and Methods: A total of 32 consecutive ankles (32 patients), with posterior malleolar fragment involving 15%-25% of the joint surface, were enrolled in the study. Patients were divided into 2 groups according to whether the fragment was fixed or not (fixed: 20 ankles, non-fixed: 12 ankles). The minimum follow-up period was 12 months. Median size of the posterior malleolar fragment in the fixed and non-fixed groups were 24.6% (range, 22.3%-25.0%) and 22.1% (range, 17.4%-24.3%), respectively. Complications as well as clinical and radiographic outcomes were compared and analyzed between the two groups. Results: Clinical outcomes, including American Orthopaedic Foot & Ankle Society (p=0.501), visual analogue scale (p=0.578), and ankle range of motion (p=0.552), showed no difference between groups at the final follow-up. No differences were obtained in the radiographic outcomes, including joint stepoff (p=0.289) and fragment gap (p=0.289). Complications, including 1 case of delayed union and 1 case of wound infection, were reported in the fixed group. Conclusion: Clinical outcomes and radiographic outcomes of the non-fixation group were satisfactory and comparable to the fixation group. Our results indicate that anatomical reduction with small-sized posterior malleolar fragment in ankle trimalleolar fractures is sufficient for satisfactory outcomes, without the need for additional internal fixation.

      • 조 혼합비율별 오메기떡 품질특성 및 항산화활성

        고지연, 우관식, 이재생, 정태욱, 윤영호 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

        This study was conducted to investigate the quality characteristics and antioxidant activities of Omegidduk by addition ratio of glutinous foxtail millet ‘Samdachal’ and waxy rice. The tested treatments of added ratio of ‘Samdachal’ in waxy rice dough were 0%, 10%, 30%, 50%, 70% and 100%. The contents of protein, crude fat, ash and mineral components in Omegidduk made by glutinous foxtail millet ‘Samdachal’ were higher than waxy rice one as addition amounts of ‘Samdachal’ were increased. The contents of polyphenol, flavonoid, ABTS and DPPH radical scavenging activities of Omegidduk were the same tendency. The color values of Omegidduk by treatments decreased L-value and b-value as added amounts of ‘Samdachal’ in waxy rice were increased and the Omegidduk by treatments showed the characteristics of ‘Samdachal’ color after 30% added. The Hardness of Omegidduk of just after processing were similar between treatments, but those showed higher values by addition ratio of ‘Samdachal’ increasing after defrost. In sensory evaluation of Omegidduk treatments, the addition of over 70% of ‘Samdachal’ in Omegidduk were showed lower value in overall acceptability. As the results, 30~50% of addition of glutinous foxtail millet ‘Samdachal’ in the view of heathy function and overall acceptability.

      • KCI등재

        Production of Human Serum Albumin in Chloroplast-Transformed Tobacco Plants

        Ko, Suk-Min,Kim, Hyun-Chul,Yoo, Byung-Ho,Woo, Je-Wook,Chung, Hwa-Jee,Choi, Dong-Woog,Liu, Jang-R. The Korean Society of Plant Biotechnology 2006 식물생명공학회지 Vol.33 No.4

        Human serum albumin (HSA) is the most abundant protein in plasma and is the most often used intravenous protein in many human therapies. However, HSA is currently extracted only from plasma because commercially feasible recombinant expression systems are not available. This study attempted to develop an efficient system for recombinant HSA production by chloroplast transformation of tobacco. A HSA cDNA was isolated from a cDNA library constructed with human liver tissue. Chloroplast transformation vectors were constructed by introducing various regulatory elements to HSA regulatory sequences. Vectors were delivered by particle bombardment into leaf explants and chloroplast-transformed plants were subsequently regenerated into whole plants. Southern blot analysis confirmed that the HSA cDNA was incorporated between rps12 and orf70B of the chloroplast genome as designed. Western blot analysis revealed that hyper-expression and increasing the stability of HSA were achieved by modification of the regulatory sequences using the psbA5'UTRs in combination with elements of the 14 N-terminal amino acids of the GFP and the FLAG tag. However, only plants transformed with the vector containing all of these elements were able to accumulate HSA.

      • KCI등재후보

        The Analysis of Risk Factors in No Thumb Test in Total Knee Arthroplasty

        Jee Hyoung Kim,Song Lee,Dong Oh Ko,Chang Wook Yoo,Tae Hwan Chun,Jung Soo Lee 대한정형외과학회 2011 Clinics in Orthopedic Surgery Vol.3 No.4

        Background: We would like to analyze the risk factors of no thumb test among knee alignment tests during total knee arthroplastysurgery. Methods: The 156 cases of total knee arthroplasty by an operator from October 2009 to April 2010 were analyzed according topreoperative indicators including body weight, height, degree of varus deformity, and patella subluxation and surgical indicatorssuch as pre-osteotomy patella thickness, degree of patella degeneration, no thumb test which was evaluated after medial prepatellaincision and before bone resection (1st test), no thumb test which was evaluated with corrective valgus stress (2nd test, Jtest), and the kind of prosthesis. We comparatively analyzed indicators affecting no thumb test (3rd test). Results: There was no relation between age, sex, and body weight and no thumb test (3rd test). Patellar sulcus angle (p = 0.795),patellar congruence angle (p = 0.276) and preoperative mechanical axis showed no relationship. The 1st no thumb test (p = 0.007)and 2nd test (p = 0.002) showed signifi cant relation with the 3rd no thumb test. Among surgical indicators, pre-osteotomy patellathickness (p = 0.275) and degeneration of patella (p = 0.320) were not relevant but post-osteotomy patellar thickness (p = 0.002)was relevant to no thumb test (3rd test). According to prosthesis, there was no signifi cance with Nexgen (p = 0.575). However,there was signifi cant correlation between Scorpio (p = 0.011), Vanguard (p = 0.049) and no thumb test (3rd test). Especially, Scorpiohad a tendency to dislocate the patella, but Vanguard to stabilize the patella. Conclusions: No thumb test (3rd test) is correlated positively with 1st test, 2nd test, and post-osteotomy patella thickness. Therefore, the more patella osteotomy and the prosthesis with high affi nity to patellofemoral alignment would be required for correctpatella alignment.

      • 차조 품종별 오메기떡 이화학적 특성 및 항산화활성

        고지연, 우관식, 이재생, 정태욱, 윤영호 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

        This study was conducted to investigate the quality characteristics and antioxidant activities of Omegidduk by glutinous foxtail millet varieties for evaluation optimum foxtail variety. The tested varieties for Omegidduk were Samdachal, Kyeonggwan 1, Kyeonggwan 2, and waxy rice as control. The contents of protein, crude fat, ash and mineral components of Omegidduk made by glutinous foxtail millet varieties were higher than those of waxy rice regardless of varieties. Glutinous foxtail millet Omegidduk were increased by protein content 1.3~1.5 fold, crude ash 12.4~14.0 fold, calcium 1.6~2.0 fold, and magnesium 5.3~8.6 fold than waxy rice Omegidduk. The contents of polyphenol and flavonoid of Omegidduk made by Samdachal, Kyeonggwan 1, and Kyeonggwan 2 were increased by 3.8~6.5 fold, and 3.2~5.3 fold. ABTS and DPPH radical scavenging activities of Omegidduk made by glutinous foxtail millet varieties were increased by 11.6~19.0 fold and 48.0~75.6 fold, respectively, compared to waxy rice that. Among the foxtail millet varieties, all the investigated components of Samdachal Omegidduk were higher than Kyeonggwan 1, and Kyeonggwan 2. The color values of Omegidduk among treatments, Samdachal Omegidduk showed the lowest L-value and b-value. As the results, Samdachal was evaluated as the most suitable glutinous foxtail millet variety in the view of quality characteristics and antioxidant activities.

      • KCI등재

        도정도와 볶음시간에 따른 수수차의 이화학적 특성

        고지연(Jee Yeon Ko),우관식(Koan Sik Woo),송석보(Seuk Bo Song),서혜인(Hye In Seo),김현영(Hyun Young Kim),김정인(Jung In Kim),이재생(Jae Saeng Lee),정태욱(Tae Wook Jung),김기영(Ki Young Kim),곽도연(Do Yeon Kwak),오인석(In Seok Oh) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.11

        항산화성 높은 수수의 식품으로서의 이용기술을 개발하여 새로운 소비수요를 창출하고자 도정도가 다른 수수를 이용하여 볶음차를 제조하고 이러한 수수차 제조과정이 수수의 항산화성분 및 항산화활성에 미치는 변화를 살펴보았다. 미도정(0%), 최소 도정(5%), 도정(20%)으로 도정 정도가 다른 수수를 1~10분간 볶음처리하여 항산화성분 및 활성 변화를 살펴본 결과 수수 볶음차의 총 폴리페놀, 플라보노이드 및 탄닌 함량과 DPPH 및 ABTS radical 소거활성은 볶음시간이 길어질수록 증가하다가 7~9분 사이 가장 높은 값을 나타내고 이후 약간 감소하는 경향이었다. 수수의 도정도별로는 미도정 수수를 볶음처리한 수수차에서 페놀성 화합물 및 항산화활성이 총 폴리페놀 12.95 mg GAE/g, 플라보노이드 7.43 ㎎ CE/g 및 DPPH 및 ABTS radical 소거활성 19.97 및 33.11 ㎎ TE/g으로 가장 높았으며, 다음으로 최소 도정 수수 볶음차, 도정 수수 볶음차의 순이었다. 이와는 다르게 도정도별 수수 볶음차의 열수 침출물의 항산화활성은 도정 수수 볶음차에서 가장 높았고, 다음으로 최소 도정 수수 볶음차, 미도정 수수 볶음차의 순이었으며, 이는 수수 볶음차 열수 침출액의 색도 및 탁도와 같은 경향으로 도정 수수 볶음차의 경우 종피의 손상에 의해 오히려 열수에 의한 색소 및 항산화성 물질의 용출이 증가한 것으로 생각되었다. In this study, sorghum teas were prepared from Sorghum bicolor L. Moench subjected to different types of milling (no milling (0%), minimum milling (5%), and milling (20%)) and pan-firing times (1 to 10 minutes), and its quality characteristics were investigated. With regards to milling type, total polyphenolic, flavonoid, tannin contents, and antioxidant activities of ethanol extracts from both no milling sorghum and its tea were highest, followed by minimum milling and milling in decreasing order. Crude nitrogen content was highest in milling sorghum and its tea regardless of pan-firing time. With regards to pan-firing time, total polyphenolic, flavonoid, tannin contents, and antioxidant activities of sorghum teas increased with increasing pan-firing time regardless of milling type. The highest DPPH and ABTS radical scavenging activities were observed at a pan-firing time between 7 to 9 minutes. In contrast to ethanol extracts of pan-fried sorghum tea, antioxidant activities, chromaticity, and turbidity of hot water leaching liqueur of sorghum tea were highest in milling sorghum tea, followed by minimum milling and no milling sorghum tea.

      • KCI등재

        N Use Efficiency and Nitrate Leaching by Fertilization Level and Film Mulching in Sesame Cultivated Upland

        Dong-Wook Lee,Ki-Do Park,Chang-Young Park,Il-Soo Son,Ui-Gum Kang,Jee-Yeon Ko,Kang-Bo Shim,Young-Son Cho,Sung-Tae Park 韓國作物學會 2007 Korean journal of crop science Vol.52 No.3

        This study was conducted to evaluate the effect of slow release fertilizers (SRF), crotonylidene diurea (CDU) and latex coated urea (LCU), on nitrogen (N) use efficiency (NUE) and nitrate-N leaching in a silty clay loam soil under polyethylene film mulching (PFM) for sesame cultivation. In PFM plot, concentrations of NO3-N and NH4-N in SRF applied soils were less than that in the urea plot during the whole growing period. However, NO3-N and NH4-N in all the non-mulched plots (NM) were not significantly different. Urea-N in soil treated with SRF was higher than urea plot until 50 days after application and was comparable in all the treatments after 50 days. NO3-N concentrations in leached solution in 21 days after urea fertilization in PFM and NM were 26 mg L-1 and 83 mg L-1 , respectively. However, NO3-N in leached solution at applied CDU and LCU was less than that of urea similar to nitrate concentration in soil. NO3-N in leached solution in applied CDU and LCU in 44 days after application was about 25% lower than that urea plot and PFM, while the NO3-N concentration of CDU and LCU treatment in NM did not changed. Application of SRF increased the yield of sesame and N recovery compared to urea and there was a little difference between SRF and N levels. In conclusion, application of 80% N level with SRF increased N recovery and reduced nitrate leaching without reduction of yields compared with urea application.

      • Effect of Organic Fertilizer on Yield of Sesame and Movement of Nitrogen and Carbon in Soil under Sesame Mulching Cultivation

        Dong-Wook Lee,Ki-Do Park,Chang-young Park,Chang-Hoon Lee,Jee-Yeon Ko,Ui-Gum Kang,Sung-Tae Park 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

        This study evaluated the effect of organic fertilizer on the yield of sesame and the movement of nitrogen and carbon in soil under mulching cultivation. The seven fertilizer treatments investigated are as follows: 1) 8-4-9 kg N-P2O5-K2O 10a-1-control, 2) 80% of control treatment N from mixed expeller cake (MEC)-MEC 80%, 3) 100% of control treatment N from MEC-MEC 100%, 4) 80% of control treatment N from mixed organic fertilizer (MOF)MOF 80%, 5) 100% of control treatment N from MOF-MOF 100%, 6) 80% of control treatment N from latex coated urea complex fertilizer (LCU)-LCU 80%, and 7) no nitrogen. The soil mineral N available in the applied organic MEC and MOF fertilizers was less than the available N in the applied urea fertilizer during the early growth stage of sesame but the amount was observed to increase with time. The concentration of nitrate-N in leached solution 35 days after application was highest at 75 mg l-1 in the control, followed in descending order by MEC 100%, MOF 100%, LCU 80%, MOF 80% and MEC 80%. The concentration of nitrate-N in the leachate in all treatments was observed to rapidly decrease 45 days after fertilizer application and recorded at less than 20 mg l-1 65 days after application. The concentration of dissolved organic carbon in the leached solution and organic carbon in the soil of the organic fertilizer plots was higher than in the urea- and LCU-fertilized plots during the whole growing period. The yields of sesame applied with MEC and MOF organic fertilizers were 2 to 7% higher than those fertilized with urea. The amount of retained mineral N or organic carbon in the soil is higher in the LCU and organic fertilizer plots than in the urea-fertilized treatments, which resulted in the reduction of nitrate leaching, increasing N efficiency and sesame yield.

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