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Yang, Tae-Jin,Kim, Jung-Sun,Lim, Ki-Byung,Kwon, Soo-Jin,Kim, Jin-A,Jin, Mina,Park, Jee Young,Lim, Myung-Ho,Kim, Ho-Il,Kim, Seog Hyung,Lim, Yong Pyo,Park, Beom-Seok Hindawi Publishing Corporation 2005 Comparative and functional genomics Vol.6 No.3
<P>A complete genome sequence provides unlimited information in the sequenced organism as well as in related taxa. According to the guidance of the Multinational Brassica Genome Project (MBGP), the Korea Brassica Genome Project (KBGP) is sequencing chromosome 1 (cytogenetically oriented chromosome #1) of <I>Brassica rapa</I>. We have selected 48 seed BACs on chromosome 1 using EST genetic markers and FISH analyses. Among them, 30 BAC clones have been sequenced and 18 are on the way. Comparative genome analyses of the EST sequences and sequenced BAC clones from <I>Brassica</I> chromosome 1 revealed their homeologous partner regions on the <I>Arabidopsis</I> genome and a syntenic comparative map between <I>Brassica</I> chromosome 1 and <I>Arabidopsis</I> chromosomes. <I>In silico</I> chromosome walking and clone validation have been successfully applied to extending sequence contigs based on the comparative map and BAC end sequences. In addition, we have defined the (peri)centromeric heterochromatin blocks with centromeric tandem repeats, rDNA and centromeric retrotransposons. In-depth sequence analyses of five homeologous BAC clones and an <I>Arabidopsis</I> chromosomal region reveal overall co-linearity, with 82% sequence similarity. The data indicate that the <I>Brassica</I> genome has undergone triplication and subsequent gene losses after the divergence of <I>Arabidopsis</I> and <I>Brassica</I>. Based on in-depth comparative genome analyses, we propose a comparative genomics approach for conquering the <I>Brassica</I> genome. In 2005 we intend to construct an integrated physical map, including sequence information from 500 BAC clones and integration of fingerprinting data and end sequence data of more than 100 000 BAC clones. The sequences have been submitted to GenBank with accession numbers: 10 204 BAC ends of the KBrH library (CW978640–CW988843); KBrH138P04, AC155338; KBrH117N09, AC155337; KBrH097M21, AC155348; KBrH093K03, AC155347; KBrH081N08, AC155346; KBrH080L24, AC155345; KBrH077A05, AC155343; KBrH020D15, AC155340; KBrH015H17, AC155339; KBrH001H24, AC155335; KBrH080A08, AC155344; KBrH004D11, AC155341; KBrH117M18, AC146875; KBrH052O08, AC155342.</P>
Lim, Ki‐,Byung,Yang, Tae‐,Jin,Hwang, Yoon‐,Jung,Kim, Jung Sun,Park, Jee‐,Young,Kwon, Soo‐,Jin,Kim, JinA,Choi, Beom‐,Soon,Lim, Myung‐,Ho,Jin, Mina,Kim, Ho Blackwell Publishing Ltd 2007 The Plant journal Vol.49 No.2
<P><B>Summary</B></P><P>We report the identification and characterization of the major repeats in the centromeric and peri‐centromeric heterochromatin of <I>Brassica rapa.</I> The analysis involved the characterization of 88 629 bacterial artificial chromosomes (BAC) end sequences and the complete sequences of two BAC clones. We identified centromere‐specific retrotransposons of <I>Brassica</I> (CRB) and various peri‐centromere‐specific retrotransposons (PCRBr). Three copies of the CRB were identified in one BAC clone as nested insertions within a tandem array of 24 copies of a 176 bp centromeric repeat, CentBr. A complex mosaic structure consisting of nine PCRBr elements and large blocks of 238 bp degenerate tandem repeats (TR238) were found in or near a derivative of 5S–25S rDNA sequences. The chromosomal positions of selected repeats were determined using <I>in situ</I> hybridization. These revealed that CRB is a major component of all centromeres in three diploid <I>Brassica</I> species and their allotetraploid relatives. However, CentBr was not detected in the most distantly related of the diploid species analyzed, <I>B. nigra</I>. PCRBr and TR238 were found to be major components in the peri‐centromeric heterochromatin blocks of four chromosomes of <I>B. rapa.</I> These repetitive elements were not identified in <I>B. oleracea</I> or <I>B. nigra</I>, indicating that they are A‐genome‐specific.</P>
Beom Jin Lim,Jin Yong Shin,Si-Gyun Roh,Nae-Ho Lee,Yoon Kyu Chung Korean Cleft Palate-Craniofacial Association 2023 Archives of Craniofacial Surgery Vol.24 No.4
Background: Free tissue transfer is the preferred method of reconstructing head and neck defects, with a success rate of approximately 95%. Although flap failure is uncommon, it has a major impact on patient morbidity and diminishes quality of life, making it is important to investigate the causes of flap failure. Methods: This retrospective chart review analyzed patients who underwent free tissue transfer during head and neck reconstruction at a single institution between 2016 and 2021. Results: During the study period, 58 patients underwent 60 free flap procedures. Revision surgery was needed in 14 patients. Subsequent free flap surgery was performed in one patient, and three free flaps (5%) could not be salvaged. Cardiovascular disease was significantly associated with flap failure, and venous congestion (thrombosis) was the most common reason for revision surgery. Conclusion: Cardiovascular disease clearly emerged as a factor related to the failure of free flap surgery, and this issue warrants particular attention in patients for whom free tissue transfer is planned.
Characterization of rDNAs and Tandem Repeats in the Heterochromatin of Brassica rapa
Beom-Seok Park,임기병,Hans de Jong,Tae-Jin Yang,Jee-Young Park,Soo-Jin Kwon,Jung Sun Kim,Myung-Ho Lim,Jin A Kim,Mina Jin,Yong-Moon Jin,Seog Hyung Kim,임용표,방재욱,Ho-Il Kim 한국분자세포생물학회 2005 Molecules and cells Vol.19 No.3
We describe the morphology and molecular organization of heterochromatin domains in the interphase nuclei, and mitotic and eiotic chromosomes, of Brassica rapa, using DAPI staining and fluorescence in situhybridization (FISH) of rDNA and ericentromere tandem repeats. We have developed a simple method to distinguish the centromeric regions of mitotic metaphase chromosomes by prolonged irradiation with UV light at the DAPI excitation wavelength. Application of this bleached DAPI band (BDB) karyotyping method to the 45S and 5S rDNAs and 176 bp centromere satellite repeats distinguished the 10 B. rapa chromosomes. We further characterized the centromeric repeat sequences in BAC end sequences. These fell into two classes, CentBr1 and CentBr2, occupying the centromeres of eight and two chromosomes, respectively. The centromere satellites encompassed about 30% of the total chromosomes, particularly in the core centromere blocks of all the chromosomes. Interestingly, centromere length was inversely correlated with chromosome length. The morphology and molecular organization of heterochromatin domains in interphase nuclei, and in mitotic and meiotic chromosomes, were further characterized by DAPI staining and FISH of rDNA and CentBr. The DAPI fluorescence of interphase nuclei revealed ten to twenty conspicuous chromocenters, each composed of the heterochromatin of up to four chromosomes and/or nucleolar organizing regions.