Chemical profile and antioxidant activity of peel of Yellowball, a novel citrus variety

이선,조성호,안지현,정성만,김동신,김상숙,박석만,윤수현,한승갑,김현진 한국식품저장유통학회 2023 한국식품저장유통학회지 Vol.30 No.2

Yellowball (Citrus hybrid cv. Yellowball ) is a new citrus hybrid between Haruka (C. tamurana × natsudaidai ) and Kiyomi (C. unshiu × sinensis) and is known to possess strong antioxidant activity. However, detailed information on the antioxidant components of its peel has not yet been reported. This study evaluated the antioxidant activity of the peel and identified the antioxidant components by fractionating a methanolic extract of Yellowball peels using liquid-liquid extraction with n-hexane, ethyl ether (ether), ethyl acetate (EA), butanol, and water. The phenolic contents and antioxidant activities of the n-hexane, ether, and EA fractions were higher than those of the other fractions, and these fractions were further separated by semi-preparative high-performance liquid chromatography (HPLC). Four antioxidant peaks, EA1, EA2, EA3, and He1, were isolated and analyzed using ultra-performance liquid chromatography–quadrupole-time- of-flight mass spectrometry (UPLC-Q-TOF MS). Sinapoyl glucoside and hesperidin were identified in EA2 and EA3, respectively, and a polymethoxylated flavone (PMF) complex (5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone, natsudaidain, tetrameth- oxyflavone, and tangeretin) was identified in He1. A compound in EA1 with m/z 223.0246 [M-H] could not be identified and was named unknown2. The antioxidant activity of unknown2 (IC50=69.17 μg/mL) was similar to that of Trolox, which was noted as a major antioxidant in Yellowball peel. Further studies on the antioxidant capacity of Yellowball peel are required; however, these results provide a foundation for using Yellowball peel as an antioxidant.

Antioxidant and free radical scavenging activities of Cleome rutidosperma

Anindya Bose,Sumanta Mondal,Jayanta Kumar Gupta,Tirtha Ghosh,Debabrata Debbhuti,Sudam Si 경희대학교 융합한의과학연구소 2008 Oriental Pharmacy and Experimental Medicine Vol.8 No.2

The study was aimed at evaluating the antioxidant and free radical scavenging activities of ethanolic extract and its fractions of Cleome rutidosperma. The antioxidant activity, reducing power, total phenolic content, total flavonoid content, superoxide anion scavenging activity, nitric oxide anion scavenging activity, in vitro antilipid peroxidation activity and in vitro nonenzymatic hemoglobin glycosylation were studied. The results obtained in the study indicate that Cleome rutidosperma is a potential source of natural antioxidant. All the parameters were found to be concentration dependent and increased with increasing amounts of sample. Flavonoids, phenolic compound like tannins, terpenoids may be responsible for the antioxidant activity of the plant. Variation of solubility parameters in various models may be attributed to non-linearity of activity of ethanol extract fractions models. Further investigation on the isolation and identification of antioxidant component(s) in the plant may lead to chemical entities with potential for clinical use. The study was aimed at evaluating the antioxidant and free radical scavenging activities of ethanolic extract and its fractions of Cleome rutidosperma. The antioxidant activity, reducing power, total phenolic content, total flavonoid content, superoxide anion scavenging activity, nitric oxide anion scavenging activity, in vitro antilipid peroxidation activity and in vitro nonenzymatic hemoglobin glycosylation were studied. The results obtained in the study indicate that Cleome rutidosperma is a potential source of natural antioxidant. All the parameters were found to be concentration dependent and increased with increasing amounts of sample. Flavonoids, phenolic compound like tannins, terpenoids may be responsible for the antioxidant activity of the plant. Variation of solubility parameters in various models may be attributed to non-linearity of activity of ethanol extract fractions models. Further investigation on the isolation and identification of antioxidant component(s) in the plant may lead to chemical entities with potential for clinical use.

A Mixture of Curcumin, Vitamin C, and E Prolongs the Antioxidant Effect to Beyond That of Each Component Alone in Vivo

Hee Young Jeon,Jeong Kee Kim,Ji Eun Lee,Hyun Jung Shin,Sang Jun Lee 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.6

This study aimed to investigate the alterations in plasma antioxidant activity after the consumption of a single oral dose of curcumin, vitamin C, and E administered individually or in combination to (ⅰ) assess possible synergies or antagonism between the antioxidants and (ⅱ) determine the optimal composition of the antioxidant mixture such that the duration of action is prolonged to beyond that of individual antioxidants. Each antioxidant was administered to male Sprague-Dawley rats, and blood samples were drawn at different time points up to 180 min to measure the plasma total antioxidant capacity (TAC). Five antioxidant compositions (M1-M5) were evaluated to assess the possible synergies or antagonisms among them and to determine the optimal composition of the antioxidant mixture. Blood samples were collected up to 360 min post-consumption. A single oral dose of individual antioxidants significantly increased the TAC values; however, the time to reach the peak TAC value varied. Among the 5 antioxidant compositions, M2 exhibited the highest and most prolonged antioxidant effect in plasma; this was greater than the proportional sum of the effects of the individual antioxidants in the composition. This result indicates a synergistic interaction among antioxidants in the optimal composition M2.

Enhanced physical stability and chemical durability of sulfonated poly(arylene ether sulfone) composite membranes having antioxidant grafted graphene oxide for polymer electrolyte membrane fuel cell applications

Kim, Kihyun,Bae, Jungmoon,Lim, Min-Young,Heo, Pilwon,Choi, So-Won,Kwon, Heock-Hoi,Lee, Jong-Chan Elsevier 2017 Journal of membrane science Vol.525 No.-

<P><B>Abstract</B></P> <P>Sulfonated poly(arylene ether sulfone) (SPAES) composite membranes were prepared using antioxidant grafted graphene oxide as a filler for polymer electrolyte membrane fuel cell (PEMFC) applications. SPAES was synthesized <I>via</I> condensation polymerization of 4,4′-dihydroxybiphenyl, 4,4′-dichlorodiphenylsulfone and 3,3′-disulfonate-4,4′-dichlorodiphenylsulfone. The antioxidant grafted graphene oxide was prepared by grafting hindered amine, 4-amino-2,2,6,6-tetramethylpiperidine, onto the surface of graphene oxide (GO). The SPAES composite membrane containing the antioxidant grafted GO showed improved physicochemical properties such as mechanical strength, chemical and thermal stabilities, and proton conductivity compared to pristine SPAES membrane. In particular, the antioxidant grafted GO was much more effective in enhancing the chemical stability of SPAES than GO without the antioxidant. For example after Fenton's test, the SPAES composite membrane with grafted antioxidant showed smaller decrease of both the weight and proton conductivity values than the pristine SPAES and SPAES composite membrane with GO. The enhanced chemical stability of SPAES composite membrane with antioxidant grafted GO can be explained by the combined antioxidant effect of both grafted antioxidant functional groups and GO.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SPAES composite membranes were prepared using antioxidant grafted GO as filler. </LI> <LI> Excellent chemical and physical stabilities of SPAES composite membranes. </LI> <LI> Proton conductivity of the composite membranes was highly increased. </LI> <LI> Proton conductivity of the composite membranes after Fenton's test was enhanced. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Antioxidant Capacity and Polyphenolic Content of Blueberry (Vaccinium corymbosum L.) Leaf Infusions

J. Piljac-Žegarac,A. Belščak,A. Piljac 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.3

Antioxidant capacity and polyphenolic content of leaf infusions prepared from six highbush blueberry cultivars (Vaccinium corymbosum L.), one wild lowbush blueberry cultivar (Vaccinium myrtillus L.), and one commercially available mix of genotypes were determined. In order to simulate household tea preparation conditions, infusions were prepared in water heated to 95°C. The dynamics of extraction of polyphenolic antioxidants were monitored over the course of 30 minutes. Extraction efficiency, quantified in terms of the total phenol (TP) content, and antioxidant capacity of infusions, evaluated by the ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging assays, were compared with cultivar type and extraction time. The 30-minute infusions exhibited the highest TP content and antioxidant capacity according to all three assays. Wild blueberry infusion had the highest TP content (1,879mg/L gallic acid equivalents [GAE]) and FRAP values (20,050μM). The range of TP values for 30-minute infusions was 394–1,879mg/L GAE with a mean of 986mg/L GAE across cultivars; FRAP values fell between 3,015 and 20,050μM with a mean of 11,234μM across cultivars. All 30-minute infusions exhibited significant scavenging capacity for DPPH• and ABTS•+ radicals, comparable to different concentrations of catechin, gallic acid, and 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid. Overall, tested infusions showed significant reducing capacity as well as radical scavenging potential, which places blueberry leaf tea high on the list of dietary sources of antioxidants.

Low‑density lipoprotein‑antioxidant flavonoids and a phenolic ester from Plectranthus hadiensis var. tomentosus

지현선,이 화,모은진,김언희,김영호,박호용,정태숙 한국응용생명화학회 2019 Applied Biological Chemistry (Appl Biol Chem) Vol.62 No.5

To investigate the effects of extraction solvents and drying methods on Plectranthus hadiensis var. tomentosus quality, eight compounds were isolated and the content of active compounds with their antioxidant activities were compared. Compounds 1 and 2 were known antioxidants, whereas the low-density lipoprotein (LDL)-antioxidant activities of compounds 3, 5, 6, and 7 are reported for the first time, with IC50 values of 2.5, 3.8, 22.8, and 53.7 μM, respectively. Our analysis of 30‒95% ethanol extracts from freeze- and air-dried leaves and stems revealed a relationship between extract composition and antioxidant activity. The 95% ethanol extracts of freeze-dried stems (FDS) exhibited highest phenolic and flavonoid content, which were 1.40 and 2.67 times, respectively, greater than those of air-dried stems (ADS), and very high LDL-antioxidant and DPPH radical scavenging activities, which may have resulted from the phenolic ester rosmarinic acid (2), a major component of FDS extracts and potent antioxidant. In contrast, the 95% ethanol extracts of ADS exhibited relatively low antioxidant activity, possibly owing to the low antioxidant activity of the main components ayanin (7) and (+)-plectranthone (8). These results are important for the development of P. hadiensis var. tomentosus as an effective natural antioxidant material.

Pomegranate extract on eroded dentin: antioxidant action, bond strength and morphology of the adhesive interface after aging

Cortez Thiago Vinícius,Cerqueira Nathália Mancioppi,Gallas Julia Adornes,Oliveira Wanderley Pereira,Corona Silmara Aparecida Milori,Souza-Gabriel Aline Evangelista 대한치과보존학회 2024 Restorative Dentistry & Endodontics Vol.49 No.1

Objectives This study aimed to evaluate the effect of pomegranate solution (Punica granatum) on eroded dentin through antioxidant action, shear bond strength (SBS) and interface morphology. Materials and Methods The 10% pomegranate peel extract was prepared by the lyophilization method. Punicalagin polyphenol was confirmed by high-performance liquid chromatography. Antioxidant activity was evaluated by capturing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. For the SBS, 48 dentin fragments were divided into sound or eroded, and subdivided according to the pretreatment (n = 12): water or P. granatum. The surfaces were restored with self-etch adhesive and a bulk-fill resin (Ecosite; DMG). The SBS was done immediately (24 hours) and after thermal cycling + water storage (12 months). For scanning electron microscopy, 48 dentin fragments (24 sound and 24 eroded) received the same treatments as for SBS (n = 6), and they were analyzed after 24 hours and 12 months. Results The P. granatum had antioxidant action similar (p = 0.246) to the phenolic standard antioxidants. After 24 hours, eroded dentin had lower SBS than sound dentin (p < 0.001), regardless of the pretreatment. After 12 months, P. granatum maintained the SBS of sound dentin (13.46 ± 3.42 MPa) and eroded dentin (10.96 ± 1.90 MPa) statistically similar. The lowest values were found on eroded dentin treated with water (5.75 ± 1.65 MPa) (p < 0.001). P. granatum on eroded dentin caused peritubular demineralization and hybrid layer with resin tags. Conclusions The pomegranate extract had antioxidant action and preserved the adhesive interface of the eroded dentin. Objectives This study aimed to evaluate the effect of pomegranate solution (Punica granatum) on eroded dentin through antioxidant action, shear bond strength (SBS) and interface morphology. Materials and Methods The 10% pomegranate peel extract was prepared by the lyophilization method. Punicalagin polyphenol was confirmed by high-performance liquid chromatography. Antioxidant activity was evaluated by capturing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. For the SBS, 48 dentin fragments were divided into sound or eroded, and subdivided according to the pretreatment (n = 12): water or P. granatum. The surfaces were restored with self-etch adhesive and a bulk-fill resin (Ecosite; DMG). The SBS was done immediately (24 hours) and after thermal cycling + water storage (12 months). For scanning electron microscopy, 48 dentin fragments (24 sound and 24 eroded) received the same treatments as for SBS (n = 6), and they were analyzed after 24 hours and 12 months. Results The P. granatum had antioxidant action similar (p = 0.246) to the phenolic standard antioxidants. After 24 hours, eroded dentin had lower SBS than sound dentin (p < 0.001), regardless of the pretreatment. After 12 months, P. granatum maintained the SBS of sound dentin (13.46 ± 3.42 MPa) and eroded dentin (10.96 ± 1.90 MPa) statistically similar. The lowest values were found on eroded dentin treated with water (5.75 ± 1.65 MPa) (p < 0.001). P. granatum on eroded dentin caused peritubular demineralization and hybrid layer with resin tags. Conclusions The pomegranate extract had antioxidant action and preserved the adhesive interface of the eroded dentin.

Antioxidant Activity and Protective Effects of Uncaria rhynchophylla Extracts on t-BHP-induced Oxidative Stress in Chang Cells

김연숙,Jin-Woo Hwang,Seong-Eun Kim,Ee Hwa Kim,전유진,문상호,Byongtae Jeon,박표잠 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.6

Uncaria rhynchophylla (UR) is widely used as a traditional Chinese herbal medicine. However, there are few studies on antioxidant activity of UR extracts. The study was aimed at determining the antioxidant activity, the contents of polyphenol and flavonoid of UR extracts. Various assays were employed to evaluate the antioxidant property of water and ethanol extracts from the UR,compared to those of the other natural and synthetic antioxidants. UR extracts had high total phenolic contents in both the water (160 ± 2.32 mg GAE/g extracts) and ethanol extracts (190.2 ± 3.16 mg GAE/g extracts). In addition, total flavonoid contents were high in both the water extracts (154 ± 1.47 mg CE/g extracts) and ethanol extracts (184.2 ± 2.41 CE/g extracts). Specially, DPPH radical scavenging activity of ethanol extracts from UR were similar with vitamin C as a positive control. In addition, antioxidant capacity in ferric reducing antioxidant power (FRAP) were higher than that for BHT, which was used as a positive control. The antioxidant activity of extracts from UR showed stronger activity than those of vitamin C and α-tocopherol in ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods. The ethanol extracts of UR protected on H2O2-induced DNA damage. In addition,cytoprotective and anti-apoptotic effect of ethanol extracts from UR was also investigated in t-BHP-induced hepatotoxicity. Therefore, these results indicate that UR extracts have antioxidant properties through its ability to enhance the cell viability, mediation of production of ROS. The UR extracts could be suitable as an antioxidant in the food industry.

天然 抗酸化劑의 動向과 方向에 關한 硏究

김인숙(Kim In-Sook) 한국인체미용예술학회 2004 한국인체미용예술학회지 Vol.5 No.2

  As natural antioxidant is extracted from plants, it is necessary that the research should be done to study the methods for producing more factors and extracting easily by the tissue culture and gene manipulation. General plants have a long growth period and they are seasonal. So, In order to overcome the difficulty, it is demanded to try different ways such as raising plants indoors, producing some special substance by gene manipulation and separating the objective substance by tissue culture. The reason why the natural substance is getting so popular is that we can get a lot of various substances and new compounds which have new functions. Natural antioxidant should be studied more based upon the researches which are done and the search for new plants.<BR>  In order to discover the first natural antioxidant from unexplored plants, studies on searching the simple methods, separating substance and refining system have to be done. Since antioxidizing vitamins from natural antioxidant are effective on anti-cancer and anti-tumor, and are relevant with suppression of other disease through free radical action, the research about physiological period should be conducted more. In the case of other natural antioxidant except antioxidizing vitamins, it should be studied concretely and systematically whether it has physiological action or not, and examined closely about the functions. When the functions are examined, natural antioxidant will be more welcome not simply as prevention from food deterioration but as physiological acting substance, and then this will be the good way to solve the financial problem for commercializing natural antioxidant.

Evaluation of antioxidant activities of various solvent extract from <i>Sargassum serratifolium</i> and its major antioxidant components

Lim, Sujin,Choi, An-Hong,Kwon, Misung,Joung, Eun-Ji,Shin, Taisun,Lee, Sang-Gil,Kim, Nam-Gil,Kim, Hyeung-Rak Elsevier 2019 Food chemistry Vol.278 No.-

<P><B>Abstract</B></P> <P> <I>Sargassum serratifolium</I> has been known to contain a high level of meroterpenoids as antioxidant components. We investigated antioxidant activities and active components in various solvent extracts from <I>S. serratifolium</I>. Ethyl acetate, ethanol, and methanol extracts showed relatively strong DPPH, ABTs, and superoxide radical scavenging activities. Hexane and ethyl acetate extract showed the strongest hydroxyl radical and reactive oxygen species (ROS), respectively, scavenging activities. Sargahydroquinoic acid (SHQA), sargachromanol (SCM) and sargaquinoic acid (SQA) were main antioxidant components in <I>S. serratifolium</I>. Ethanol extract showed the highest levels of SHQA, SCM, and SQA which comprised to be 227 ± 6.31 mg/g. SHQA and SCM exhibited stronger antioxidant capacities than SQA based on lower IC<SUB>50</SUB> values in ROS, DPPH, ABTs, and superoxide radical scavenging assays. The result showed that ethanol is the most efficient extracting solvent for the active components from <I>S. serratifolium</I> and the plant has the potential as a natural antioxidant.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>S. serratifolium</I> contain a high level of meroterpenoids as antioxidant compounds. </LI> <LI> Ethyl acetate, ethanol, and methanol extracts showed high TPC and antioxidant capacity. </LI> <LI> SHQA, SCM and SQA are main antioxidant components in <I>S. serratifolium.</I> </LI> </UL> </P>