A study on wound healing activity of Bacopa monnieri Linn. aerial parts

Tirtha Ghosh,Tapan Kumar Maity,Deepak Kumar Dash,Anindya Bose 경희대학교 융합한의과학연구소 2007 Oriental Pharmacy and Experimental Medicine Vol.7 No.2

Bacopa monnieri is being used in the traditional system of medicine for a variety of ailments. In the present study, the ethanolic extract of Bacopa monnieri aerial parts has been studied for its wound healing activity using various models in rats. Significant increase in wound contraction and skin breaking strength were observed in the excision and incision wound models respectively. There was also significant increase in hydroxyproline content, DNA content, superoxide dismutase activity, catalase activity, glutathione level and decrease in the level of thiobarbituric acid reactive substances in the 12th day post wounding tissue of experimental rats in the ethanol extract treated groups with respect to the control group. The effect of the extract was found to be comparable with the standard drug nitrofurazone. From the results it may be concluded that the plant Bacopa monnieri is endowed with significant wound healing activity, thereby justifying its use in the traditional medicine. Saponins may be responsible for the observed wound healing activity.

A study on wound healing activity of Bacopa monnieri Linn. aerial parts

Ghosh, Tirtha,Maity, Tapan Kumar,Dash, Deepak Kumar,Boss, Anindya Kyung Hee Oriental Medicine Research Center 2007 Oriental pharmacy and experimental medicine Vol.7 No.2

Bacopa monnieri is being used in the traditional system of medicine for a variety of ailments. In the present study, the ethanolic extract of Bacopa monnieri aerial parts has been studied for its wound healing activity using various models in rats. Significant increase in wound contraction and skin breaking strength were observed in the excision and incision wound models respectively. There was also significant increase in hydroxyproline content, DNA content, superoxide dismutase activity, catalase activity, glutathione level and decrease in the level of thiobarbituric acid reactive substances in the $12^{th}$ day post wounding tissue of experimental rats in the ethanol extract treated groups with respect to the control group. The effect of the extract was found to be comparable with the standard drug nitrofurazone. From the results it may be concluded that the plant Bacopa monnieri is endowed with significant wound healing activity, thereby justifying its use in the traditional medicine. Saponins may be responsible for the observed wound healing activity.

Evaluation of antitumor activity of stigmasterol, a constituent isolated from Bacopa monnieri Linn aerial parts against Ehrlich Ascites Carcinoma in mice

Tirtha Ghosh,Tapan Kumar Maity,Jagadish Singh 경희대학교 융합한의과학연구소 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.1

Cancer remains as one of the most common cause of mortality worldwide. Cancer chemoprevention by natural products is well accepted nowadays. Phytoserols are natural products, showing anticancer activity, besides other activities. The purpose of this study was to examine the antitumor and antioxidant activity of stigmasterol, a phytosterol isolated from aerial parts of Bacopa monnieri Linn. against Ehrlich Ascites Carcinoma (EAC) in swiss albino mice. The effect of stigmasterol on the growth of transplantable murine tumor, life span of EAC bearing hosts, simultaneous alterations in the hematological profile,liver biochemical parameters (lipid peroxidation, antioxidant enzymes) and histopthological studies of liver were examined. Stigmasterol decreased tumor volume, packed cell volume and viable cell count, and increased mean survival time thereby increasing life span of EAC tumor bearing mice. Hematological profile reverted to near normal levels in stigmasterol treated mice. Stigmasterol decreased the levels of lipid peroxidation and increased the levels of glutathione, superoxide dismutase and catalase in the liver of EAC bearing mice. Histopathological study of liver tissues showed that stigmasterol had significant protective effect against EAC bearing mice, which corroborates the above findings. Lactate dehydrogenase (LDH) activity in ascitic fluid increased while membrane microviscosity of the EAC cells decreased upon stigmasterol treatment indicating its effect in the membrane integrity functions. The antitumor activity of stigmasterol might be mediated through the activation of protein phosphatase 2A by ceramide causing apoptosis, as is shown by structurally similar phytosterol.

Effect of various fractions of Bacopa monnieri Linn. aerial parts on ethanol-induced hepatotoxicity in rats

Ghosh, Tirtha,Maity, Tapan Kumar,Dash, Deepak Kumar,Bose, Anindya Kyung Hee Oriental Medicine Research Center 2007 Oriental pharmacy and experimental medicine Vol.7 No.3

The ethyl acetate fraction (EAF) and n-butanol fraction (NBF) of ethanolic extract of Bacopa monnieri aerial parts were screened for hepatoprotective activity and in vivo antioxidant activity on ethanol-induced hepatotoxic rats. Ethyl acetate fraction was found to be more potent even though both the fractions were endowed with significant hepatoprotective activity. EAF and NBF were investigated for hepatoprotective activity in albino rats at 300 mg/kg, p.o. dose and compared with standard drug Silymarin (25 mg/kg, p.o.). Results show that both the fractions were effective in blunting ethanol-induced enhanced activities of serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase, level of serum bilirubin (both total and direct), liver weight loss and was also effective in reducing ethanol-induced lipid peroxidation both in vitro and in vivo. Furthermore, the fractions could also enhance ethanol-induced suppressed activities of superoxide dismutase, catalase and decreased level of reduced glutathione. Results of hepatocellular damage caused by ethanol and its recovery by EAF and NBF, suggest that they might be considered as a potential source of natural hepatoprotective agents, which could be related to the free radical scavenging properties of saponins present in high concentration in the fractions.

Hepatoprotective Activity of Ethanolic Extract of Bacopa monnieri Linn. AerialParts Against CCl4-induced Hepatotoxicity in Rats

Tirtha Ghosh,Tapan Kumar Maity,Gouri Kumar Dash,Anindya Bose,Siva Sankar Nayak,Deepak Kumar Dash 한국생약학회 2007 Natural Product Sciences Vol.13 No.1

ethanolic extract of Bacopa monnieri Linn. aerial parts were studied for its hepatoprotective efecton CCl4-induced hepatotoxic rats. The extract was found to decrease significantly CCl4-induced elevation ofSGOT, SGPT, ALP, bilirubin and total cholesterol. But, it increased HDL-cholesterol level and liver weight withrespect to CCl4 toxic rats. The extract was also found to decrease significantly the CCl4-induced elevation of lipidperoxidation and increase the activity of antioxidant enzymes (SOD and CAT) and GSH level in the liver ofextract treated rats when compared with CCl4 induced rats. Histopathological profiles showed that the extract hadsignificant protective effect against CCl4may be possible that the mechanism of hepatoprotection of the extract is due to its antioxidant effect.KeywordsBacopa monnieri L., ethanolic extract, hepatoprotective activity, antioxidant activity, histopathology.

Effect of various fractions of Bacopa monnieri Linn. aerial parts on ethanolinduced hepatotoxicity in rats

Tirtha Ghosh,Tapan Kumar Maity,Deepak Kumar Dash,Anindya Bose 경희대학교 융합한의과학연구소 2007 Oriental Pharmacy and Experimental Medicine Vol.7 No.3

The ethyl acetate fraction (EAF) and n-butanol fraction (NBF) of ethanolic extract of Bacopa monnieri aerial parts were screened for hepatoprotective activity and in vivo antioxidant activity on ethanolinduced hepatotoxic rats. Ethyl acetate fraction was found to be more potent even though both the fractions were endowed with significant hepatoprotective activity. EAF and NBF were investigated for hepatoprotective activity in albino rats at 300 mg/kg, p.o. dose and compared with standard drug Silymarin (25 mg/kg, p.o.). Results show that both the fractions were effective in blunting ethanolinduced enhanced activities of serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase, level of serum bilirubin (both total and direct), liver weight loss and was also effective in reducing ethanol–induced lipid peroxidation both in vitro and in vivo. Furthermore, the fractions could also enhance ethanol-induced suppressed activities of superoxide dismutase, catalase and decreased level of reduced glutathione. Results of hepatocellular damage caused by ethanol and its recovery by EAF and NBF, suggest that they might be considered as a potential source of natural hepatoprotective agents, which could be related to the free radical scavenging properties of saponins present in high concentration in the fractions.

Evaluation of antitumor activity of stigmasterol, a constituent isolated from $Bacopa$ $monnieri$ Linn aerial parts against Ehrlich Ascites Carcinoma in mice

Ghosh, Tirtha,Maity, Tapan Kumar,Singh, Jagadish 경희한의학연구센터 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.1

Cancer remains as one of the most common cause of mortality worldwide. Cancer chemoprevention by natural products is well accepted nowadays. Phytoserols are natural products, showing anticancer activity, besides other activities. The purpose of this study was to examine the antitumor and antioxidant activity of stigmasterol, a phytosterol isolated from aerial parts of $Bacopa$ $monnieri$ Linn. against Ehrlich Ascites Carcinoma (EAC) in swiss albino mice. The effect of stigmasterol on the growth of transplantable murine tumor, life span of EAC bearing hosts, simultaneous alterations in the hematological profile, liver biochemical parameters (lipid peroxidation, antioxidant enzymes) and histopthological studies of liver were examined. Stigmasterol decreased tumor volume, packed cell volume and viable cell count, and increased mean survival time thereby increasing life span of EAC tumor bearing mice. Hematological profile reverted to near normal levels in stigmasterol treated mice. Stigmasterol decreased the levels of lipid peroxidation and increased the levels of glutathione, superoxide dismutase and catalase in the liver of EAC bearing mice. Histopathological study of liver tissues showed that stigmasterol had significant protective effect against EAC bearing mice, which corroborates the above findings. Lactate dehydrogenase (LDH) activity in ascitic fluid increased while membrane microviscosity of the EAC cells decreased upon stigmasterol treatment indicating its effect in the membrane integrity functions. The antitumor activity of stigmasterol might be mediated through the activation of protein phosphatase 2A by ceramide causing apoptosis, as is shown by structurally similar phytosterol.

Anthelmintic, antimicrobial and antipyretic activity of various extracts of Clerodendrum infortunatum Linn. leaves

Amiya Kumar Prusty,Tirtha Ghosh,Susanta Kumar Sahu 경희대학교 융합한의과학연구소 2008 Oriental Pharmacy and Experimental Medicine Vol.8 No.4

In the present study, various extracts of Clerodendrum infortunatum leaves have been studied for its anthelmintic, antimicrobial and antipyretic activities. In the anthelmintic and antipyretic study it was observed that the benzene extract was more potent than the other two extracts (ethanol and aqueous) even though all the three extracts were endowed with both the properties. The study reveals antimicrobial activity of the extracts against the tested strains of microorganisms between concentration ranges of 75 μg/ml and 350 μg/ml and shows effectiveness more against grampositive bacteria than the gram-negative bacteria. In the present study, various extracts of Clerodendrum infortunatum leaves have been studied for its anthelmintic, antimicrobial and antipyretic activities. In the anthelmintic and antipyretic study it was observed that the benzene extract was more potent than the other two extracts (ethanol and aqueous) even though all the three extracts were endowed with both the properties. The study reveals antimicrobial activity of the extracts against the tested strains of microorganisms between concentration ranges of 75 μg/ml and 350 μg/ml and shows effectiveness more against grampositive bacteria than the gram-negative bacteria.

Effect of Ichnocarpus frutescens extract on antihyperglycemic, antihyperlipidemic and antioxidant status in streptozotocin-induced diabetic rats

Deepak K Dash,Tirtha Ghosh,Veerendra C Yeligar,K Murugesh,Siva S Nayak,Bhim C Maiti,Tapan K Maity 경희대학교 융합한의과학연구소 2007 Oriental Pharmacy and Experimental Medicine Vol.7 No.3

The present study was carried out to investigate the antihyperglycemic, antihyperlipidemic and antioxidant effect of chloroform and methanol extract of whole plant of Ichnocarpus frutescens (CEIF and MEIF) in streptozotocin (STZ)-induced diabetic rats. Administration of CEIF and MEIF orally at the dose of 200 mg/kg and 400 mg/kg body weight resulted in significant (P < 0.01) reduction in blood glucose levels. The body weights were significantly (P < 0.001) reduced in STZ-induced diabetic rats when compared to normal rats while the extracts significantly (P < 0.01) prevented the decrease in body weight in the CEIF and MEIF treated rats. The study was further undertaken to evaluate the antioxidant and antihyperlipidemic potential of CEIF and MEIF in STZ-induced diabetic rats. The increased levels of lipid peroxidation in the liver tissues of diabetic rats were significantly reverted back to normal levels and a significant increase in the activity of antioxidant enzymes such as superoxide dismutase, catalase and the level of reduced glutathione in the liver of diabetic rats after the treatment with CEIF and MEIF was noticed. These results clearly indicate that CEIF and MEIF exhibit significant antihyperglycemic, antihyperlipidemic and in vivo antioxidant activity in STZ-induced diabetic rats and the results were found to be in a dose dependent manner

Anthelmintic, antimicrobial and antipyretic activity of various extracts of Clerodendrum infortunatum Linn. leaves

Prusty, Amiya Kumar,Ghosh, Tirtha,Sahu, Susanta Kumar Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.4

In the present study, various extracts of Clerodendrum infortunatum leaves have been studied for its anthelmintic, antimicrobial and antipyretic activities. In the anthelmintic and antipyretic study it was observed that the benzene extract was more potent than the other two extracts (ethanol and aqueous) even though all the three extracts were endowed with both the properties. The study reveals antimicrobial activity of the extracts against the tested strains of microorganisms between concentration ranges of $75\;{\mu}g/ml$ and $350\;{\mu}g/ml$ and shows effectiveness more against gram-positive bacteria than the gram-negative bacteria.