Pollen Viability of Common Buckwheat (Fagopyrum esculentum Moench.) in in vitro Germination and its Application to Male Gametophyte Isolation

Woo, Sun-HeeㆍKeun-Yook ChungㆍHeung-Tae, KimㆍYong-Gu, ChoㆍHong-Sig, KimㆍBeom-Heon, Songㆍ Chul-Won, Lee and Seung-Keun Jong 忠北大學校 農業科學硏究所 2004 農業科學硏究 Vol.21 No.-

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간흡충: 충체 및 대사성 항원의 특성분석 (1)항원투여 마우스 비장조직에 대한 면역조직화학적 연구

송강원,주난영,류장근,양용석 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.2

저자들은 마우스를 실험모델로 하여 간흡충의 항원을 투여 했을 때 비장조직에 대한 CD3, CD4 및 CD8 모노클로날 항체의 반응 여부를 알아보고자 하였다. 즉, 간흡충에 대한 세포면역학적인 특성을 규명고자 하였으며 특히 비장 조직에 대한 phenotype을 관찰한 결과 다음과 같은 결과를 얻었다. 간흡충의 조항원을 면역증강제와 함께 복강 투여한 다음 일정 기간 후에 비장조직을 Avidin-biotin complex 면역조직염색을 실시한 결과 CD3에서 강한 양성 반응을 나타냈고 CD4와 CD8에서는 약한 반응을 나타냈다. 조직부위를 보면 피막, 혈관, 임파관, 백수부위와 림프구 및 대식세포의 세포막에서 양성반응을 보였다. The authors inquired into what reactions comprise the response of mice(as a model) CD3, CD4 and CD8 monoclonal antibodies in spleen tissue when injected intraperitoneally by antigens of Clonorchis sinensis. The author is objective was focused on investigating the property of cellular immunity for liver fluke. In particular, the results of having examined the phenotype of the tissue of spleen were revealed as follows: a certain length of time after having been intraperitoneally injected with antigens of Clonorchis sinensis and Freund's adjuvant, the tissue of spleen was embedded and immunohistochemically stained by the avidin-biotin complex method. A strong reaction in response to CD3, while a feeble reaction resulted from CD4 and CD8. The tissue region showed a positive reaction to all antibodies, especially from capsules, vascular areas, white pulps and membrane of blood cells.

매실 野生 酵母의 醱酵에 관한 硏究

宋寶鉉,金龍斗,李在根 順天大學校 1985 論文集 Vol.4 No.1

매실의 野生酵母를 발효 실험을 통하여 酵母의 同定, 酒精, Fusel oil 및 香氣 成分을 分析한 結果는 다음과 같다. 매실에서 발견된 野生酵母는 Saccharomyce cervisiaeSP. 5종 Pichti속 3종. Torulopsis 2종을 同定하였다. 酒精 生成力은 일발연 1호가 16%였으며 野生 酵母Ⅰ,Ⅱ.Ⅲ,Ⅳ는 각각 15.2%, 14.1%, 7.64% 및 13.5%였다. Fusel oil 生命力은 일발연 1호가 2.7%, 396호가 2.6%였으며 野生 酵母 Ⅰ,Ⅱ,Ⅲ,Ⅳ는 각각 0.5%, 0.3%, 0.3% 및 0.5%로 나타 香氣 成分은 일반연1호, 396호, 野生 酵母Ⅰ은 비슷하였으며 野生 酵母Ⅱ, Ⅲ, Ⅳ는 香氣 成分이 많은 것으로 나타났다. This study was conducted to evaluate the alcohols and flavors through fermentational using the wild yeast isolated in Mume(prunus mume s.e.t.z.)and the wild yeasts were identified, concurrently. The alcohols and flavors were analyzed by chromatography. 5 species(Sacch,cerevisiae sp.), 3 species(Pichia genus)and 2 spcecies(Torulopsis sp.)were identified. In the alcohol yielding ability test of yeasts, the yielding ability was 16% in Sacch cerevisiae, and 15.2%, 14.1%, 7.6%and 13.5% in the wild yeasts(#1,2,3,4), respectively, while in the fusel, the percentage of fusel oil was 2.7% in Sacch. cerevisiae, 2.6% in sacch. formosensis, and 0.5%, 0.3%, 0.3%and 0.8% in the wild yeasts(#1,2,3,4,)respectively. The amount of flavors made by yeasts such as Sacch cervisiae, Sacch. formosensis and wild yeast #1 was similar in each case but that of the wild yearst #2,3,4 was much more than above 3 species yeasts.

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

서울지역 특급호텔 Executive Floor의 효율적인 마케팅 전략

송대근,강용관 문화관광연구학회 2002 문화관광연구 Vol.4 No.3

Nowadays, the hotel industry is becoming large-sized and high-grade in every part including guest room, F&B and other facilities in order to satisfy variable customers' need. Especially in the part of guest room, the Executive Floor(EFL) which provides high leveled service for business travellers are contiounsly increasing in recent year. Therefore, many improvement plans which should be carried out are the followings: To know to control the human resources to realize the improvement the quality service for guests and the quality-management of EFL in hotel. Also EFL have to completed equipment for business work and strenghtening of more personalized and differentiated service. Futhermore, the competition in hotel business in focused not only on quantification but also qualification.

PCR-Restricition Fragment Length Polymorphism 방법에 의한 Borrelia burgdorferi Sensu Lato의 분류

송혜원,김홍,박상욱,엄용빈,김종배,박성언,김근희 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCINE 1999 Journal of biomedical laboratory sciences Vol.5 No.2

라임병의 원인균인 Borrelia burgdorferi에 대하여 각 균종의 표준균주와 진드기에서 추출한 DNA를 template로 PCR을 실시한 후 그 증폭산물을 Alu I으로 처리한 restriction fragment length polymorphism (RFLP) 방법으로 각 균종의 연관성을 조사하고자 하였다. 표준균주로 RFLP를 실시한 결과 B. burgdorferi sensu stricto와 B. garinii의 RFLP 형태 (50 bp, 70 bp, 150 bp)가 유사하였으며 B.afzelii에서는 다른 RFLP형태 (50bp, 110bp, 150 bp)를 관찰하였다. 그 중 B. afzelii KK-1과 B. garinii HP1은 새로운 RFLP 형태를 보여 B. afzelii와 B. garinii는 각각 2 types의 subgroup으로 분류할 수 있었다. 진드기 DNA에서 는 B. afzelii를 포함한 각 균종에 대하여 모두 유사한 RFLP 형태를 보였는데, 진드기 DNA에서 확인된 B. afzelii는 KK-1과 같은 군에 속하는 것으로 사료되었다. For the classification of B. burgdorferi sensu lato strains, PCR-restriction fragment length polymorphism (RFLP) analysis was performed. PCR was carried out with B. burgdorferi sensu lato specific primer set (BB uni set), and amplicons of 470-bp DNA were digested with Alu I. The Alu I restriction polymorphism of the amplicons provided a useful tool for identifying B. burgdorferi sensu lato strains. Both amplicons from B. burgdorferi sensu stricto and B. garinii except HP1 strain showed identical RFLP pattern (50 bp, 70 bp, and 150 bp), but amplicons from B. afzelii and B. garinii showed two types of subgroups, respectively. The result of PCR-RFLP using extracted DNAs from ticks was similar to those patterns of B. burgdorferi species including B. afzelii.

구속조건과 온도관리가 콘크리트 벽체의 균열 거동에 미치는 영향

변근주,조호진,송하원,박용운 연세대학교 산업기술연구소 1996 논문집 Vol.28 No.2

Mass concrete wall with external restraint has high possibility of thermal cracking due to thermal stress caused by heat of hydration. Specially the cracking control is the most important factor for the requirements of watertightness and durability of concrete structures. In this study, the results by finite element thermal analysis on concrete wall are compared with experimental data and verified, and then various parameters which affect thermal cracking of the wall are analyzed using the program. The parameters include cement and fly-ash contents, concrete placement volume, concrete placement temperature, and ambient temperature during concrete placement and curing. From the study it is concluded that pre-cooling method or the use of crushed ice which lower placement temperature down to 10℃ is the most effective way to control the thermal cracking of massive concrete walls.

치주조직 재생을 위한 칼슘 포스페이트 글라스가 MC3T3-E1 세포의 증식, 분화 및 석회화에 미치는 영향

이용근,송진,이상배,김경남,최성호 大韓齒科器材學會 2003 대한치과재료학회지 Vol.30 No.2

The purpose of this work was to exploring the influence of the prepared calcium phosphate glass on the proliferation, differentiation, and mineralization of the culture system of the pre-osteoblastic MC3T3-E1 cells. Polystytene was employed as a control in this experiment. Cells were cultured in a differentiated medium onto the calcium phosphate glass as well as polystyrene, and the number of cells and the protein contents were determined. To examine osteoblast differentiation, the alkaline phosphatase activity was measured. Mineralization was evaluated by staining the calcium deposit with Alizarin red. Culture onto the calcium phosphate glass exhibited no significant difference in cell proliferation compared with control group. The alkaline phosphatase activity in the experimental group, however. was enhanced In the calcium phosphate glass significantly at 10-18 days than that of the control group (p<0.05). Enhancement of bone-like tissue formation by the addition of the calcium phosphate glass was observed since 7 days. The results of the present study indicate that the prepared calcium phosphate glass promotes osteogenesis by increasing collagen synthesis and calcification of the extracellular matrix. It maybe due to greater calcium concentration in the culture medium released from the calcium phosphate glass.

생체분해성 망막압정을 이용한 망막고정에 대한 실험적 연구

김용백,민병무,김창식,박근성,김승영,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Biodegradable retinal fixation devices obtain mechnical fixation of the retina with desirable chorioretinal scarring and with the potential for local, sustained release of antimetabolites and steroids to inhibit proliferative vitreoretinopathy. We manufactured a biodegradable retinal tack with barb that was designed in order to prevent intrusion from implantation of retinal tacks. This study was carried to evaluate the efficacy for retinal fixation and the capability for sustained release of drugs with a newly designed biodegradable retinal tack Biodegradable retinal tacks were made of polymers of glycolic acids and were designed with barbs in a shape to prevent the disinsertion. Biodegradale retinal tacks are divided into 3 parts, a conical portion that is inserted into the sclera, a cylinder portion that remains in the vitreous, and a neck portion between the pin and the cylinder. The tapered conical end was manufactured to allow easy insertion through the retina and choroid into the sclera. A cylinder portion was manufactured with a tapered angle that fixes firmly into the orifice of 19 gauge spinal needle. A neck portion, 0.4 mm in diameter, was designed to prevent disinsertion from following implantation of retinal tack. The applicator was a 19 gauge spinal needle and its orifice was prepared to 15°angle to accept the tapered cylinder portion of the retinal tack. The retinal tacks, secured in the needles, were passed through the formed vitreous and inserted into the retina, choroid, and sclera and were released by pushing the internal needle, usually within 2-3mm of the medullary ray of the posterior rabbit retina A retinal tack was placed in each of 8 pigmented rabbit eyes. Slit-lamp biomicroscopy, indirect ophthalmoscopy and fundus photography were performed periodically from 1 day to 8 weeks after surgery. Eight eyes were enucleated and studied by light microscopy at 8 weeks. Biomicroscopic evaluation of the animals revealed edemas adjacent to the retinal surfaces immediately after insertion of the biodegradable retinal tacks in all the animals. These edemas disappeared after 1 week. The first noticeable change in the size of retinal tacks was shown after 2weeks. The size of the retinal tacks gradually got smaller, decreasing to about one-half at 4 weeks and about one-third at 8 weeks. All retinal tacks remained in inserted places without any movement for an 8 week period. On light microscopy, epiretinal proliferations were seen to extend into the vitreous cavity. Cellular capsules that lined the inner aspect of the scleral defect caused by tack insertion were found. However the adjacent retina had a normal cytologic appearance and architecture in all specimens. We manufactured a biodegradable retinal tack that is designed to prevent intrusion from implantation of retinal tacks. All biodegradable retinal tacks reduce in size with time, but no retinal tacks extruded from the inserted place. The newly designed biodegradable retinal tack can be used for retinal fixation and may be used as a vehicle for the introduction of pharmacologic agents to prevent the cellular events that promote proliferative vitreoretinopathy.

성인 만성 부비동염에서 내시경적 부비동 수술 전·후의 증상 호전도와 음향비강통기도 검사 결과

김용대,김재열,장근영,이형중,송시연,윤석근 영남대학교 의과대학 2002 Yeungnam University Journal of Medicine Vol.19 No.1

Background: Chronic paranasal sinusitis is one of the most common disease in the otorhinolaryngologic field. Endoscopic sinus surgery is treatment of choice in chronic paranasal sinusitis. The aim of this study was to evaluate objective and subjective treatment outcomes of endoscopic sinus surgery in adult chronic paransal sinusitis with or without polyp. Materials and Methods: We reviewed 84 adult patients underwent endoscopic sinus surgery by one surgeon from June 1999 to June 2000, prospectively. We analyzed preoperative and postoperative subjective symptom scores and acoustic rhinometric results. Results: Fifty cases were male and thirty four cases female. The average age was of 33 year-old (range: 17 to 66 years). There was significantly improvement of symptom scores in postoperative 3 months and 6 months compared with preoperative symptom scores. There was significantly increased postoperative total volume of nasal cavity. When we compared high score group with low score group, there was statistically significant improvement of symptom scores between preoperative stage and postoperative 3 months in radiologic grading group. Conclusion: Endoscopic sinus surgery is considered to be effective for the treatment of chronic paranasal sinusitis. It seems to be helpful to employ subjective symptom score system and objective total volume change of nasal cavity through acoustic rhinometric test to analyze effectiveness of endoscopic sinus surgery. In this study, the most important preoperative factor of sinus surgery outcomes is radiologic grading system.