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Undaria Pinnatifida Suppresses Nasal Inflammation via Inhibiting Eosinophil and Mast Cell Activation
Zhen Nan Yu,Chang Ho Song,Thi Van Nguyen,Juan Jin,Eui Hyeog Han,Yan Jing Fan,Hyoung Tae Kim,Ok Hee Chai 대한체질인류학회 2021 대한체질인류학회 학술대회 연제 초록 Vol.64 No.-
Undaria pinnatifida (U. pinnatifida), a brown alga commonly grown in the oceans of East Asia, has long been a part of human diet and medicine. Though U. pinnatifida has been reported to have immunomodulatory, anti-inflammatory, anti-oxidant, anti-diabetic and anti-bacterial activities, its specific effect on allergic rhinitis (AR) disease has not been clarified. In this study, the anti-allergic and anti-inflammatory effects of U. pinnatifida extract (UPE) were investigated in a mouse model of ovalbumin (OVA)-induced AR. After UPE treatment, the nasal and lung allergy symptoms, nasal mucosal swelling, and goblet cell hyperplasia were obviously ameliorated. Oral UPE regulated the balance of T helper (Th) 1/Th2 and Th17/Treg cells differentiation in AR mice in a dose-dependent manner. Accordingly, UPE attenuated the expression of Th2 cytokines (IL-4, IL-5, IL-13), Th17-related cytokine IL-17, proinflammatory cytokines (TNF-α, IL-6) and up-regulated the secretion of Th1 and Treg cytokines including IL-12, IFN-γ and IL-10 in nasal lavage fluid (NALF). Also, UPE attenuated the migration of eosinophils to the nasal mucosa and NALF by inhibiting eotaxin, an eosinophil-specific chemoattractant, as well as suppressed Nuclear factor-kappaB (NF-κB)/Signal transducer and activator of transcription 3 (STAT3), a signaling pathway in eosinophils. The levels of anti-OVA specific IgE and IgG1 were decreased, and as a result, the allergic response was attenuated by UPE via inhibiting mast cells accumulation in nasal mucosa. These results suggested that UPE may have therapeutic potential for treating AR through modulating important immune cells in the immune system such as Th1/Th2 and Th17/Treg balance, eosinophils and mast cells.
Wu Zhen-Yu,Wang Yumin,Hu Hao,Ai Xiang-Nan,Zhang Qiang,Qin Yu-Gang 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.6
Background/Aims: The involvement of long noncoding RNAs in the carcinogenesis of hepatocellular carcinoma (HCC) has been well documented by substantial evidence. However, whether cytoskeleton regulator RNA (CYTOR) could affect the progression of HCC remains unclear. Methods: The relative expression of CYTOR, miR-125a-5p and HS1-associated protein X-1 (HAX-1) mRNA in HCC cells were determined via quantitative real-time polymerase chain reaction. The viability of treated HCC cells was measured by Cell Counting Kit-8 assay. Cell apoptosis was estimated by flow cytometry analysis, assessment of caspase-9 activity and terminal deoxynucleotidyl transferase dUTP nick-end labeling staining, and Western blot of apoptosis-related proteins. The interplay between CYTOR or HAX-1 and miR-125a-5p was validated by dual-luciferase reporter assay. Results: CYTOR was upregulated and miR-125a-5p was downregulated in HCC cells. CYTOR silencing inhibited cell proliferation and promoted cell apoptosis in HepG2 and SMMC-7721 cells. miR-125a-5p was sponged and negatively regulated by CYTOR, and HAX-1 was directly targeted and negatively modulated by miR-125a-5p. Overexpression of miR-125a-5p enhanced the repressive effects of CYTOR knockdown on HCC cells, and knockdown of HAX-1 enhanced the inhibitory effects of miR-125a-5p mimics on HCC cells. Conclusions: CYTOR silencing facilitates HCC cell apoptosis in vitro via the miR-125a-5p/HAX-1 axis.
( Yun Yu ),( Xiao Jun Ouyang ),( Qing-lin Lou ),( Liu Bao Gu ),( Yong Zhen Mo ),( Gary T. Ko ),( Chun Chung Chow ),( Wing Yee So ),( Ronald Ma ),( Alice Kong ),( Nicola Brown ),( Jennifer Nan ),( Juli 대한내과학회 2012 The Korean Journal of Internal Medicine Vol.27 No.1
Background/Aims: The application of glycated hemoglobin (HbA1c) for the diagnosis of diabetes is currently under extensive discussion. In this study, we explored the validity of using HbA1c as a screening and diagnostic test in Chinese subjects recruited in Nanjing, China. Methods: In total, 497 subjects (361 men and 136 women) with fasting plasma glucose (PG) ≥ 5.6 mmol/L were recruited to undergo the oral glucose tolerance test (OGTT) and HbA1c test. Plasma lipid, uric acid, and blood pressure were also measured. Results: Using a receiver operating characteristic curve, the optimal cutoff point of HbA1c related to diabetes diagnosed by the OGTT was 6.3%, with a sensitivity and specificity of 79.6% and 82.2%, respectively, and the area under the curve was 0.87 (95% confidence interval, 0.83 to 0.92). A HbA1c level of 6.5% had a sensitivity and specificity of 62.7% and 93.5%, respectively. When comparing the HbA1c ≥ 6.5% or OGTT methods for diagnosing diabetes, the former group had significantly higher HbA1c levels and lower levels of fasting and 2-hour PG than the latter group. No significant difference was observed in the other metabolism indexes between the two groups. Conclusions: Our results suggest that HbA1c ≥ 6.5% has reasonably good specificity for diagnosing diabetes in Chinese subjects, which is in concordance with the American Diabetes Association recommendations.
Zhou, Lei,Yu, Lan,Feng, Zhen-Zhong,Gong, Xiao-Meng,Cheng, Ze-Nong,Yao, Nan,Wang, Dan-Na,Wu, Shi-Wu Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10
Background: Gastric cancer is the second leading cause of cancer-related death in Asia, and the majority type is gastric adenocarcinoma (GAC). Most GAC patients die of recurrence and metastasis. Cancer stem cells (CSCs) have been thought to be responsible for the initiation, development, metastasis, and ultimately recurrence of cancer. In this study, we aimed to investigate expression and clinical significance of CSCs markers, CD133 and Lgr5, and vasculogenic mimicry (VM) in primary GAC. Materials and Methods: Specimens from 261 Chinese patients with follow-up were analyzed for CD133, Lgr5 protein expression and VM by immunohistochemical and histochemical staining. The Pearson Chi's square test was used to assess the associations among the positive staining of these markers and clinicopathological characteristics. Postoperative overall survival time was were studied by univariate and multivariate analyses. Results: In GAC tissues, positive rates of 49.0%, 38.7%, and 26.8% were obtained for CD133, Lgr5, and VM, respectively. The mean score of microvessel density (MVD) was $21.7{\pm}11.1$ in GAC tissues. There was a significantly difference between the positive and negative groups. There was a positive relationship between the VM, the expression of CD133 and Lgr5, and the score of MVD and the grades of tumor, lymph node metastasis, TNM stages (all p<0.05). The overall mean survival time of the patients with CD133, Lgr5, VM, and MVD (${\geq}22$) positive expression was lower than that of patients with negative expression. The score of MVD, positive expression of CD133 and VM were independent prognostic factors of GAC (p<0.05). Conclusions: VM, and expression of CD133, Lgr5, and the score of MVD are related to grades of tumor, lymph node metastasis, TNM stages, and overall mean survival time. It is suggested that CSCs and VM could play an important role in the evolution of GAC.
Preparation and Characterization of Inclusion Complex between β-Cyclodextrin and Polylactic Acid
Song Ya Nan,Zhou Yu Fang,Zhen Wei Jun 한국고분자학회 2015 폴리머 Vol.39 No.2
The inclusion complexes (ICs) between polylactic acid (PLA) and β-cyclodextrin (CD) were prepared by coprecipitation method in this work. The orthogonal experiments were designed to investigate the influence of different factors on the formation of inclusion complexes. The results suggested that the optimum scheme of inclusion compounds could be obtained when the feeding ratio of CD to PLA (wt%) was 20:1, stirring speed was 6 kr/min and the stirring time was 30 min. The structures and properties of the inclusion complexes were characterized by <SUP>1</SUP>H NMR, FTIR, DSC, FT-Raman, XRD and TGA. The DSC results demonstrated that the crystallization behavior of the inclusion complexes nearly disappeared. It was found that β-CD-PLA inclusion complex had a better thermal stability compared with the neat PLA. The model of the inclusion complexes was proposed on the basis of XRD, <SUP>1</SUP>H NMR and DSC results.
Study on suppression strategy of jet lag effect in melt electrowriting
Zhongfei Zou,Yu Wang,Zhen Shen,Nan Luo 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.9
The melt electrowriting (MEW) has broad applications in regenerative medicine and micro-nano electronics. It is an efficient micro-nano scale additive manufacturing technology; however, the fiber jet lag effect of MEW limits the deposition precision and resolution of fiber shape. In this study, the principle of the jet lag effect is studied to overcome the defect of printed structure distortion and improve the ability to print complex structures. A mathematical model of trailing fiber trajectory is established. The study covers jet lag and liquid rope coiling analysis at different speeds. A strategy is adopted by introducing a buffer zone at the corner of the printing structure. The printing path is subdivided and optimized to suppress the influence of jet lag. The results show that the deposited fibers' corner radius is around 63.81±5.66 μm, which is significantly smaller than that of unoptimized groups. Finally, by utilizing the improved printing paths, the high-precision and complex structures are printed, which demonstrates the feasibility of optimizing the buffer zone for the MEW.
( Zhong Wen Chen ),( Yin Bing Zhang ),( Xaing Jun Chen ),( Xiao Liu ),( Zhen Wang ),( Xi Kun Zhou ),( Ji Qiu ),( Nan Nan Zhang ),( Xiu Teng ),( Yong Qiu Mao ),( Chang Yong Liu ),( Yu Quan Wei ),( Jion 대한피부과학회 2015 Annals of Dermatology Vol.27 No.2
Background: Psoriasis is an autoimmune disease that is caused by a shift in the Th1/Th2 balance toward Th1- dominant immunity. It has been established as an effective treatment to counteract psoriasis by subcutaneous injection of recombinant interleukin (IL)-4, and IL-4 gene therapy by topical transdermal penetration has shown its antipsoriatic effect in mice. Retinoic acid (RA) and dimethylsulfoxide can increase the efficiency of gene transfection in the topical transdermal delivery system. Objective: We investigated whether RA could improve anti-psoriasis efficiency using IL-4 expression plasmid pORF-mIL-4 (pIL-4) via transdermal delivery system in K14-vascular endothelial growth (K14- VEGF) factor transgenic mice. Methods: After pretreatment with RA, plasmid pIL-4 in 10% dimethylsulfoxide was applied to the ear skin by topical transdermal penetration. Hematoxylin- eosin staining and immunohistochemistry were performed with ear samples to evaluate anti-psoriasis efficiency in mice. Results: The psoriasis pathological features were relieved and psoriasis-associated factors were significantly reduced. Conclusion: Our results reveal that topical application of pIL-4 in dimethylsulfoxide by transdermal delivery with RA pretreatment can improve psoriasis significantly.(Ann Dermatol 27(2) 121∼127, 2015)
( Zhong Wen Chen ),( Yin Bing Zhang ),( Xaing Jun Chen ),( Xiao Liu ),( Zhen Wang ),( Xi Kun Zhou ),( Ji Qiu ),( Nan Nan Zhang ),( Xiu Teng ),( Yong Qiu Mao ),( Chang Yong Liu ),( Yu Quan Wei ),( Jion 대한피부과학회 2015 Annals of Dermatology Vol.27 No.3
Background: Psoriasis is an autoimmune disease that is caused by a shift in the Th1/Th2 balance toward Th1- dominant immunity. It has been established as an effective treatment to counteract psoriasis by subcutaneous injection of recombinant interleukin (IL)-4, and IL-4 gene therapy by topical transdermal penetration has shown its antipsoriatic effect in mice. Retinoic acid (RA) and dimethylsulfoxide can increase the efficiency of gene transfection in the topical transdermal delivery system. Objective: We investigated whether RA could improve anti-psoriasis efficiency using IL-4 expression plasmid pORF-mIL-4 (pIL-4) via transdermal delivery system in K14-vascular endothelial growth (K14- VEGF) factor transgenic mice. Methods: After pretreatment with RA, plasmid pIL-4 in 10% dimethylsulfoxide was applied to the ear skin by topical transdermal penetration. Hematoxylin- eosin staining and immunohistochemistry were performed with ear samples to evaluate anti-psoriasis efficiency in mice. Results: The psoriasis pathological features were relieved and psoriasis-associated factors were significantly reduced. Conclusion: Our results reveal that topical application of pIL-4 in dimethylsulfoxide by transdermal delivery with RA pretreatment can improve psoriasis significantly. (Ann Dermatol 27(2) 121∼127, 2015)