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Anti-allergic Effects of Caffeine in an Allergic Rhinitis Mouse Model
Yan Jing Fan,Chun Hua Piao,Thi Van Nguyen,Zhen Nan Yu,Ok Hee Chai,Chang Ho Song 대한체질인류학회 2020 대한체질인류학회지 Vol.33 No.1
Caffeine (1, 3, 7-trimethylxanthine) is one of the most widely consumed pharmacologically active products worldwide. Caffeine exhibits various pharmacological activities in central nervous, cardiovascular, and respiratory systems. Additionally, caffeine exhibited anti-inflammatory effects in lipopolysaccharide-challenged rats. However, to our knowledge, the potential anti-inflammatory activity of caffeine in allergic rhinitis (AR) has not yet been investigated. Therefore, in this study, we aimed to examine the anti-allergic effects of caffeine in ovalbumin (OVA)-induced AR in mice. We showed that caffeine attenuated the nasal symptoms, including rubbing and sneezing. It reduced the thickness of the nasal mucosa and alleviated goblet cell hyperplasia in the nasal mucosa. In addition, caffeine ameliorated the inflammation in the lungs and decreased OVA-specific immunoglobulin E (IgE) and IgG1 levels in the serum. It also reduced T helper type 2 (Th2) cytokine (interleukin (IL)-4 and IL-5) levels and elevated Th1 cytokine (IL-12 and IFN-γ) levels in the nasal lavage fluid. Collectively, we suggest that caffeine might have therapeutic effects in AR owing to its anti-inflammatory activities.
Allium hookeri Inhibits Inflammation in OVA-induced Allergic Rhinitis in Mice via NF-κB Pathway
Yan Jing Fan,Juan Jin,Chun Hua Piao,Thi Van Nguyen,김유석,채옥희,송창호 대한체질인류학회 2023 해부·생물인류학 (Anat Biol Anthropol) Vol.36 No.3
Allium hookeri (A. hookeri) has been used as a traditional medicine for many years in the Southeast Asian region. Multiple investigations have demonstrated its role in preventing of microbial infection, immune regulation, coronary thrombosis, and atherosclerosis has been demonstrated by several studies. Therefore, this study aims to investigate the suppressing effect of A. hookeri on the ovalbumin (OVA)-induced allergic rhinitis (AR) murine model. In this study, AR was induced in BALB/c mice by using OVA emulsified in aluminum on days 1, 8 and 15. Then, nasally challenged with OVA on days 22 to 28. On days 16 to 28, OVA-induced mice were treated either with A. hookeri (50, 100, 200 mg/kg) or Dexamethasone (Dex, 2.5 mg/kg) by oral gavage. On days 22 to 28, mice received either A. hookeri (50, 100, 200 mg/kg) or Dexamethasone (Dex, 2.5 mg/kg) an hour before the OVA challenge. The mice in the control group were not sensitized, treated, or tested. In this study, A. hookeri ameliorated nasal symptoms include rubbing and sneezing; reduced thickness of nasal mucosa; alleviated goblet cell hyperplasia and eosinophil infiltration in the nasal mucosa; decreased levels of OVA-specific IgE and OVA-specific IgG1 in serum; and suppressed Th2 (IL-4, IL-5, and IL-13) cytokines level in nasal lavage fluid (NALF). In addition, A. hookeri recovered the production of Th1 (IL-12 and IFN-γ) cytokines level in NALF, inhibited the inflammation through NF-κB pathway. Collectively, our results suggest that A. hookeri effectively protects against AR by regulating Th1/Th2 cytokines imbalance and NF-κB mechanisms. These observations suggest A. hookeri as a potential therapeutic option for AR.
Caffeine ameliorates allergic rhinitis in an ovalbumin-induced murine model
Yan Jing Fan,Eui Hyeog Han,Hyoung Tae Kim,Zhen Nan Yu,Thi Van Nguyen,Juan Jin,Ok Hee Chai,Changho Song 대한체질인류학회 2021 대한체질인류학회 학술대회 연제 초록 Vol.64 No.-
Caffeine is one of the most widely consumed pharmacologically active products worldwide. Caffeine exhibits various pharmacological activities in central nervous, cardiovascular, and respiratory systems. Additionally, caffeine exhibited anti-inflammatory effects in lipopolysaccharide-challenged rats. However, the potential anti-inflammatory activity of caffeine in allergic rhinitis (AR) has not been investigated yet. Therefore, we aimed to examine the anti-allergic effects of caffeine in ovalbumin (OVA)-induced AR mice in this study. Briefly, BALB/c mice were sensitized and challenged with OVA. Caffeine (5, 10, 20 mg/kg) and dexamethasone (Dex, 2.5 mg/kg) were treated by intranasal instillation of 20 µL/nasal cavity for 13 days. In this study, caffeine attenuated the nasal symptoms, including rubbing and sneezing. Caffeine ameliorated the inflammation in the lungs. In addition, caffeine reduced the thickness of the nasal mucosa, alleviated goblet cell hyperplasia and reduced mast cell recruitment in the nasal mucosa. Caffeine decreased OVA-specific immunoglobulin (Ig) E and IgG1 levels in the serum, whereas increased the level of OVA-specific IgG2a. Caffeine also reduced T helper (Th) 2 cytokines (interleukin(IL)-4, IL-5 and IL-13) and Th17 family related factors (IL-17, IL-6, retinoic-related orphan receptor gamma, signal transducer and activator of transcription-3 and phospho-signal transducer and activator of transcription-3) levels and elevated Th1 cytokines (IL-12 and interferon gamma) levels in the nasal lavage fluid. However, the level of malondialdehyde was down-regulated, the levels of superoxide dismutase, nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 were up-regulated in the caffeine treatment. Collectively, we suggest that caffeine might have therapeutic effects on AR.
Crocetin Induces Cytotoxicity in Colon Cancer Cells Via p53-independent Mechanisms
Li, Cai-Yan,Huang, Wen-Feng,Wang, Qun-Li,Wang, Fan,Cai, E.,Hu, Bing,Du, Jia-Cheng,Wang, Jing,Chen, Rong,Cai, Xiao-Jing,Feng, Jing,Li, Hui-Hui Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8
Objective: Crocin has been proposed as a promising candidate for cancer chemoprevention. The purpose of this investigation was to investigate the chemopreventive action and the possible mechanisms of crocin against human colon cancer cells in vitro. Methods: Cell proliferation was examined using MTT assay and the cell cycle distribution fractions were analyzed using fow cytometric analysis after propidium iodide staining. Apoptosis was detected using theTUNEL Apoptosis Detection Kit with laser scanning confocal microscope. DNA damage was assessed using the alkaline single-cell gel electrophoresis assay, while expression levels of p53, cdk2, cyclinA and P21 were examined by Western blot analysis. Results: Treatment of SW480 cells with crocetin (0.2, 0.4, 0.8 mmol/L) for 48 h signifcantly inhibited their proliferation in a concentration-dependent manner. Crocetin (0.8 mmol/L) signifcantly induced cell cycle arrest through p53-independent mechanisms accompanied by P21 induction. Crocetin (0.8 mmol/L) caused cytotoxicity in the SW480 cells by enhancing apoptosis and decreasing DNA repair capacity in a time-dependent manner. Conclusions: This report provides evidence that crocetin is a potential anticancer agent, which may be used as a chemotherapeutic drug.
Study on the optimization of the decolorization of orange essential oil
Jing-Nan Ren,Yan Zhang,Gang Fan,Mei-Ping Wang,Lu-Lu Zhang,Zi-Yu Yang,Siyi Pan 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.4
The effects of diatomite, activated clay and acticarbon on the decolorization of orange essential oil were investigated. Single factor and orthogonal tests were performed to determine the optimum discoloring conditions. The results showed that the activated clay exhibited the most satisfactory effect on discoloring. Then it was used as the decolorizer for the decolorization of orange essential oil. The highest decolorization rate (84.5%) was obtained using 10% activated clay at 60 C for 30 min. The contents of oxygenated compounds (linalool and citral) increased from 1.4 to 3.1% after decolorization. Sensory assessment revealed that the orange essential oil after decolorization using activated clay had a mellow and characteristic orange aroma. Chromaticity analysis showed that it had excellent transparency and yellow color under the optimized condition. Thus, decolorization with activated clay could maintain the quality and prolong the storage of orange essential oil.
IL-35 Over-expression is Associated with Genesis of Gastric Cancer
Fan, Yong-Gang,Zhai, Jing-Ming,Wang, Wei,Feng, Bing,Yao, Guo-Liang,An, Yan-Hui,Zeng, Chao Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7
Overexpression of interleukin (IL)-35 has been found in a variety of malignancies, but the expression status in gastric cancer has yet to be elucidated clearly. In the present study, positive expression of EBI3 and p35 was 63.3% and 70.0% of cases, respectively. EBI3 expression was strongly related with larger tumor size and invasion depth (P<0.05). Similarly, expression of p35 was also correlated with larger tumor size (P<0.05). These results indicate that IL-35 might be involved in growth of gastric cancer. Interestingly, EBI3 and p35 expressions were positive correlated with Ki-67 expression. Moreover, EBI3 immunoreactivity was associated with Bcl-2 staining. Our data suggest IL-35 is correlated with genesis of gastric cancer by regulating growth and apoptosis.