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      • The catalytic core of DEMETER guides active DNA demethylation in <i>Arabidopsis</i>

        Zhang, Changqing,Hung, Yu-Hung,Rim, Hyun Jung,Zhang, Dapeng,Frost, Jennifer M.,Shin, Hosub,Jang, Hosung,Liu, Fang,Xiao, Wenyan,Iyer, Lakshminarayan M.,Aravind, L.,Zhang, Xiang-Qian,Fischer, Robert L. National Academy of Sciences 2019 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.116 No.35

        <P><B>Significance</B></P><P>Flowering plants reproduce via a unique double-fertilization event, producing the zygote and the nutritive endosperm. The genome of the central cell, the precursor of the endosperm, undergoes extensive demethylation prior to fertilization. This epigenetic reconfiguration, directed by the DEMETER (DME) glycosylase at thousands of loci in <I>Arabidopsis</I>, differentiates the epigenetic landscapes of parental genomes and establishes parent of origin-specific expression of many imprinted genes in endosperm essential for seed development. However, how DME is targeted to various locations remains unknown. Here we show that the multidomain DME is organized into 2 functional regions: the C-terminal region, which guides localization and catalysis, and the N-terminal region, which likely recruits chromatin remodelers to facilitate demethylation within heterochromatin.</P><P>The <I>Arabidopsis</I> DEMETER (DME) DNA glycosylase demethylates the maternal genome in the central cell prior to fertilization and is essential for seed viability. DME preferentially targets small transposons that flank coding genes, influencing their expression and initiating plant gene imprinting. DME also targets intergenic and heterochromatic regions, but how it is recruited to these differing chromatin landscapes is unknown. The C-terminal half of DME consists of 3 conserved regions required for catalysis in vitro. We show that this catalytic core guides active demethylation at endogenous targets, rescuing <I>dme</I> developmental and genomic hypermethylation phenotypes. However, without the N terminus, heterochromatin demethylation is significantly impeded, and abundant CG-methylated genic sequences are ectopically demethylated. Comparative analysis revealed that the conserved DME N-terminal domains are present only in flowering plants, whereas the domain architecture of DME-like proteins in nonvascular plants mainly resembles the catalytic core, suggesting that it might represent the ancestral form of the 5mC DNA glycosylase found in plant lineages. We propose a bipartite model for DME protein action and suggest that the DME N terminus was acquired late during land plant evolution to improve specificity and facilitate demethylation at heterochromatin targets.</P>

      • KCI등재

        HDRE: Coverage Hole Detection with Residual Energy in Wireless Sensor Networks

        Yun-zhou Zhang,Xiaohua Zhang,Wenyan Fu,Zeyu Wang,Honglei Liu 한국통신학회 2014 Journal of communications and networks Vol.16 No.5

        Coverage completeness is an important indicator forquality of service in wireless sensor networks (WSN). Due to limitedenergy and diverse working conditions, the sensor nodes havedifferent lifetimes which often cause network holes. Most of the existingmethods expose large limitation and one-sidedness becausethey generally consider only one aspect, either coverage rate or energyissue. This paper presents a novel method for coverage holedetection with residual energy in randomly deployed wireless sensornetworks. By calculating the life expectancy of working nodesthrough residual energy, we make a trade-off between network repaircost and energy waste. The working nodes with short lifetimeare screened out according to a proper ratio. After that, the locationsof coverage holes can be determined by calculating the jointcoverage probability and the evaluation criteria. Simulation resultshows that compared to those traditional algorithms without considerationof energy problem, our method can effectively maintainthe coverage quality of repairedWSN while enhancing the life spanof WSN at the same time.

      • KCI등재

        Optimal Area and Intrinsic Coherent Resonance of Neurons due to Ion Channel Noise under an external Stimulus

        Zhengzhen Zhang,Shaowen Zeng,Wenyan Tang,Jinlin Hu,Rongfeng Wang,Liming Zhou,Shangyou Zeng 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.61 No.1

        In this paper, we study the intrinsic coherence resonance of neurons due to ion channel noise under an external stimulus. We find that the external stimulus cannot only increase the spiking coherence of neurons but also increase the optimal area of neuronal membrane for maximal spiking coherence. The optimal membrane area is approximately 3 μm<sup>2</sup> without the external stimulus. However, when the external stimulus is 6 μA/cm<sup>2</sup>, the optimal membrane area is approximately 400 μm<sup>2</sup>. Because 400 μm<sup>2</sup> is the area of a common neuronal soma, our finding has a realistic meaning. Under the external stimulus, common neurons have an optimized conformation to guarantee maximal spiking coherence and the best sub-threshold signal encoding.

      • KCI등재

        Persistent Malfunction of Glymphatic and Meningeal Lymphatic Drainage in a Mouse Model of Subarachnoid Hemorrhage

        Tinglin Pu,Wenyan Zou,Weixi Feng,Yanli Zhang,Linmei Wang,Hongxing Wang,Ming Xiao 한국뇌신경과학회 2019 Experimental Neurobiology Vol.28 No.1

        Subarachnoid hemorrhage (SAH) is a devastating cerebrovascular event that often is followed by permanent brain impairments. It is necessary to explore the pathogenesis of secondary pathological damages in order to find effective interventions for improving the prognosis of SAH. Blockage of brain lymphatic drainage has been shown to worsen cerebral ischemia and edema after acute SAH. However, whether or not there is persistent dysfunction of cerebral lymphatic drainage following SAH remains unclear. In this study, autologous blood was injected into the cisterna magna of mice to establish SAH model. One week after surgery, SAH mice showed decreases in fluorescent tracer drainage to the deep cervical lymph nodes (dcLNs) and influx into the brain parenchyma after injection into the cisterna magna. Moreover, SAH impaired polarization of astrocyte aquaporin-4 (AQP4) that is a functional marker of glymphatic clearance and resulted in accumulations of Tau proteins as well as CD3+ , CD4+ , and CD8+ cells in the brain. In addition, pathological changes, including microvascular spasm, activation of glial cells, neuroinflammation, and neuronal apoptosis were observed in the hippocampus of SAH mice. Present results demonstrate persistent malfunction of glymphatic and meningeal lymphatic drainage and related neuropathological damages after SAH. Targeting improvement of brain lymphatic clearance potentially serves as a new strategy for the treatment of SAH.

      • Design and Implementation of Energy Saving Controller for Air-Conditioner in Building

        Liang Zhao,Jili Zhang,Yue Shen,Wenyan Wu 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.8

        This paper has completed the hardware design of STM32F103 system circuit, infrared code learning module, human body infrared sensor module, indoor temperature and humidity sensor module, completes the software design of learning air conditioning remote control code, indoor temperature and humidity sensor module, also completes the energy-saving controller hardware and software debugging. Finally, this paper takes split type air conditioning units in a conference room as the control object (including indoor lighting system) to complete the energy-saving controller in air conditioner electrical system design, installation and debugging, completes the air conditioning start-stop and infrared temperature control code learning test. The test results show that energy-saving controller has realized infrared automatic perception of human activity, machine start-stop code self-learning and temperature settings and other functions.

      • KCI등재

        Damage and Deterioration Mechanism of Coal Gangue Mixed Pumice Aggregate Concrete Under Freeze–Thaw Cycles

        Wen Li,Hailong Wang,Wenyan Zhang 한국콘크리트학회 2024 International Journal of Concrete Structures and M Vol.18 No.1

        The world is facing the problem of depletion of natural sand and gravel resources, and a large amount of coal gangue solid waste is produced in Inner Mongolia, China, which has low utilization rate and causes ecological pollution. In order to improve the gangue in the mining infrastructure construction of a wide range of application prospects, the use of coal gangue as the coarse aggregate of pumice concrete is of great significance. Inner Mongolia is a cold region, and gangue mixed aggregate concrete (MFC) will certainly face the damage caused by freeze–thaw cycles. Therefore, design gangue by different volume replacement rate (0%, 20%, 40%, 60%, 80%, 100%) to replace pumice coarse aggregate. The results show that with the increase of gangue substitution rate, the mass loss rate, relative dynamic elastic modulus, and peak stress of MFC decrease, but the trend of peak strain increases. It is mainly attributed to the less Al2O3 and SiO2 content of gangue, which makes the MFC hydration products decrease with the increase of substitution rate and more original microcracks and pores in the specimens. In addition, the damage model of MFC was established by using Weibull statistical distribution theory and the principle of LEMAITRE equivalent effect variation assumption, and the damage evolution characteristics were explored by combining the experimental results. It can provide the theoretical basis for the application of MFC in cold regions.

      • KCI등재

        Assessment of the microbial diversity of Chinese Tianshan tibicos by single molecule, real-time sequencing technology

        Chenxia Cao,Qiangchuan Hou,Wenyan Hui,Laiyu Kwok,Heping Zhang,Wenyi Zhang 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.1

        Chinese Tianshan tibico grains were collectedfrom the rural area of Tianshan in Xinjiang province,China. Typical tibico grains are known to consist ofpolysaccharide matrix that embeds a variety of bacteria andyeasts. These grains are widely used in some rural regionsto produce a beneficial sugary beverage that is slightlyacidic and contains low level of alcohol. This work aimedto characterize the microbiota composition of ChineseTianshan tibicos using the single molecule, real-timesequencing technology, which is advantageous in generatinglong reads. Our results revealed that the microbiotamainly comprised of the bacterial species of Lactobacillushilgardii, Lactococcus raffinolactis, Leuconostoc mesenteroides,Zymomonas mobilis, together with a Guehomycespullulans-dominating fungal community. The data generatedin this work helps identify beneficial microbes inChinese Tianshan tibico grains.

      • Effect of Ligustilide Supplement on Physical Fitness of Athletes

        Xiaojuan Gao,Shuai Yang,Meng Liu,Yawen Jiang,Li Wenyan,Rui Ma,Zijian Zhao,Xuelin Zhang 아시아건강운동학회 2020 Journal of Asian Society for Health & Exercise Vol.2 No.2

        PURPOSE: In this study, high content ligustilide plant extract was supplemented to monitor the changes of physical fitness and physiological function of athletes, and to explore the effect of ligustilide plant extract on physical function and physical fitness of athletes. METHODS: Thirty five high-level basketball players from Zhengzhou University were selected and divided into two groups randomly: nutritional supplement group (S, n = 18) and control group (C, n=17). This study was approved by the Ethics Committee of Zhengzhou University. Informed consent was obtained from each subject. The intervention period was 8 weeks, six days per week. The training method adopted the small cycle plate training scheme. We measured changes of the scores of rating of perceived exertion (RPE), physical fitness test (15m running, 30m running, 400m running) and routine blood test (mainly including red blood cell count, hemoglobin concentration and average erythrocyte hemoglobin concentration, etc.) before and after training. RESULTS: In group S, the percentage of RPE (grade 6-11) and RPE (grade 12-18) before experiment was 37.5% and 62.5%. After 8 weeks of experiment, 62.5% of RPE (grade 6-11) and 37.5% of RPE (grade 12-18). In group C, 14% of RPE (grade 6-11), 86% of slight RPE (grade 12-18), and 100% of RPE (grade 12-18) after 8 weeks of experiment. It was found that after 8 weeks of training, the number of red blood cells, the concentration of hemoglobin and the average concentration of red blood cell hemoglobin in group S were significantly higher than those before the experiment (p <0.05). According to the comparison of the physical fitness test results of the athletes in group S, it was found that the performance of 400m after 4 weeks and 8 weeks was significantly higher compared with pre-experiment (p<0.05). The changes of 30m and 15m were not significant different (p>0.05). The 400-meter test performance of athletes in group C was significantly higher than that before the experiment only after 8 weeks of training (p<0.05). CONCLUSIONS: Ligustilide supplementation can increase the number of red blood cell and hemoglobin concentration of athletes and has a positive impact on the physical fitness of athletes and the recovery of fatigue.

      • KCI등재

        A high-resolution timescale for the Miocene Shanwang diatomaceous shale lagerstätte (China): development of Wavelet Scale Series Analysis for cyclostratigraphy

        Jifeng Yu,Xinlong Pang,Wenzhao Fu,Jason Hilton,Mingmei Liang,Zongkai Jiang,Xiuli Zhao,Wenyan Qiao,Suo Shi,Diandong Zhang,Huitao Cao,Haibo Jia,Yadong Wang,Xiaoke Hu,Rui Zhang 한국지질과학협의회 2021 Geosciences Journal Vol.25 No.5

        The Miocene aged Shanwang Formation from the Shanwang National Geopark in China represents a succession of lacustrine diatomaceous shales containing an abundant and diverse biota with lagerstätte fossilization of soft tissues. To date, the Shanwang Formation has not been investigated for cyclostratigraphy nor has it been dated with high precision methods. Now we use thorium data as a paleoenvironmental and paleoclimatic proxy to conduct a detailed cyclostratigraphic analysis. A new and simple cyclostratigraphic method, Wavelet Scale Series Analysis (WSSA) is developed to recognize Milankovitch cycles. A total of three short eccentricity and fifteen precession cycles are identified; obliquity cycles are not apparent. In the sedimentary succession, the corresponding precession and short eccentricity cycles are 1.17 m and 4.98 m thick respectively, with this verified by Correlation Coefficient (COCO) analysis and Multitaper-Method (MTM) spectral analysis. We estimate the studied interval was deposited over a duration of 0.3 Myr with a depositional rate of c. 5.7 cm/kyr. Paleomagnetic and radio isotope dating data shows that the diatomaceous shale was deposited during Chron C5En, which places it at approximately 18.5 Ma during the Burdigalian stage of the Early Miocene, rather than in the Middle Miocene as previously thought. The Shanwang lagerstätte biota therefore predates the Middle Miocene Climate Optimum (MMCO) and did not form within it. The geological time scale with a high resolution of 20 kyr was set accordingly.

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