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      • KCI등재

        Few-Layered MoS2 Nanostructures for Highly Efficient Visible Light Photocatalysis

        Dan Li,Jianwei Li,Caiqin Han,Xinsheng Zhao,Haipeng Chu,Wenyan Lei,Xinjuan Liu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.10

        Few-layered MoS2 nanostructures were successfully synthesized by a simple hydrothermal method without the addition of any catalysts or surfactants. Their morphology, structure and photocatalytic activity were characterized by X-ray diffraction, scanning electron microscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy, electrochemical impedance spectra and UV-Vis absorption spectroscopy, respectively. These results show that the MoS2 nanostructures synthesized at 180℃ exhibit an optimal visible light photocatalytic activity (99%) in the degradation of Rhodamine B owing to the relatively easier adsorption of pollutants, higher visible light absorption and lower electron–hole pair recombination.

      • KCI등재

        Damage and Deterioration Mechanism of Coal Gangue Mixed Pumice Aggregate Concrete Under Freeze–Thaw Cycles

        Wen Li,Hailong Wang,Wenyan Zhang 한국콘크리트학회 2024 International Journal of Concrete Structures and M Vol.18 No.1

        The world is facing the problem of depletion of natural sand and gravel resources, and a large amount of coal gangue solid waste is produced in Inner Mongolia, China, which has low utilization rate and causes ecological pollution. In order to improve the gangue in the mining infrastructure construction of a wide range of application prospects, the use of coal gangue as the coarse aggregate of pumice concrete is of great significance. Inner Mongolia is a cold region, and gangue mixed aggregate concrete (MFC) will certainly face the damage caused by freeze–thaw cycles. Therefore, design gangue by different volume replacement rate (0%, 20%, 40%, 60%, 80%, 100%) to replace pumice coarse aggregate. The results show that with the increase of gangue substitution rate, the mass loss rate, relative dynamic elastic modulus, and peak stress of MFC decrease, but the trend of peak strain increases. It is mainly attributed to the less Al2O3 and SiO2 content of gangue, which makes the MFC hydration products decrease with the increase of substitution rate and more original microcracks and pores in the specimens. In addition, the damage model of MFC was established by using Weibull statistical distribution theory and the principle of LEMAITRE equivalent effect variation assumption, and the damage evolution characteristics were explored by combining the experimental results. It can provide the theoretical basis for the application of MFC in cold regions.

      • KCI등재

        Efficient Visual Place Recognition by Adaptive CNN Landmark Matching

        ( Yutian Chen ),( Wenyan Gan ),( Yi Zhu ),( Hui Tian ),( Cong Wang ),( Wenfeng Ma ),( Yunbo Li ),( Dong Wang ),( Jixian He ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.11

        Visual place recognition (VPR) is a fundamental yet challenging task of mobile robot navigation and localization. The existing VPR methods are usually based on some pairwise similarity of image descriptors, so they are sensitive to visual appearance change and also computationally expensive. This paper proposes a simple yet effective four-step method that achieves adaptive convolutional neural network (CNN) landmark matching for VPR. First, based on the features extracted from existing CNN models, the regions with higher significance scores are selected as landmarks. Then, according to the coordinate positions of potential landmarks, landmark matching is improved by removing mismatched landmark pairs. Finally, considering the significance scores obtained in the first step, robust image retrieval is performed based on adaptive landmark matching, and it gives more weight to the landmark matching pairs with higher significance scores. To verify the efficiency and robustness of the proposed method, evaluations are conducted on standard benchmark datasets. The experimental results indicate that the proposed method reduces the feature representation space of place images by more than 75% with negligible loss in recognition precision. Also, it achieves a fast matching speed in similarity calculation, satisfying the real-time requirement.

      • Studies on the Technology of the Pulse Glare Sterilization and its Sterilization effects

        정문언 ( Ding Wenyan ),리가강 ( Li Jiaqiang ),장로 ( Jianglu ),서강저 ( Xu Jiangning ) 한국농공학회 2007 한국농공학회 학술대회초록집 Vol.2007 No.-

        Basing on the microorganism effect theories of the strong pulse light and according to the factors of the pulse light, the equipment of bacterium and detecting was made by the strong pulse light technology. The general capacitance circuit was improved and the pulse generating circuit is consisting of two powers. Used this equipment the primary experiment about the bacterium effects was finished. The result shows that the flash frequency、input voltage、 bacterium liquid depth、 bacterium liquid transparency are the main factors and its sterilizing effect is remarkable. This equipment can be applied in processing and storage engineering of agricultural products.

      • KCI등재

        Drought stress reduces the feeding preference of Nilaparvata lugens due to the accumulation of abscisic acid and callose deposition in rice

        Liao Guangrong,Li Jitong,Fan Wenyan,Chen Lin,Liu Jinglan 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

        The incidence of drought stress in plants has been increasing due to global warming, and the phytohormone abscisic acid (ABA) induces the formation of physical barriers in plants, such as callose accumulation. The brown planthopper (BPH; Nilaparvata lugens Stål) occurs throughout Asia and feeds on rice, but the effects of drought stress on BPH feeding remain unclear. In this study, we observed changes in callose formation and ABA content in rice during drought stress. ABA content and the relative expression of ABA synthesis genes OsNCED3 and OsNCED5 were higher in drought-stressed rice than the non-stressed control. Similarly, the expression levels of callose synthesis genes and callose deposition were significantly higher in drought-stressed rice as compared to non-stressed plants, and this impacted BPH feeding. Our results indicated that rice resistance to BPH increased during drought stress due to the accumulation of callose and increasing ABA levels. Our findings provide a basis for understanding BPH feeding performance on rice during drought stress and offer novel insights relative to control during periods of water shortage.

      • KCI등재

        Lane Detection Based on Inverse Perspective Transformation and Kalman Filter

        ( Yingping Huang ),( Yangwei Li ),( Xing Hu Wenyan Ci ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.2

        This paper proposes a novel algorithm for lane detection based on inverse perspective transformation and Kalman filter. A simple inverse perspective transformation method is presented to remove perspective effects and generate a top-view image. This method does not need to obtain the internal and external parameters of the camera. The Gaussian kernel function is used to convolute the image to highlight the lane lines, and then an iterative threshold method is used to segment the image. A searching method is applied in the top-view image obtained from the inverse perspective transformation to determine the lane points and their positions. Combining with feature voting mechanism, the detected lane points are fitted as a straight line. Kalman filter is then applied to optimize and track the lane lines and improve the detection robustness. The experimental results show that the proposed method works well in various road conditions and meet the real-time requirements.

      • KCI등재

        Overexpression of StNHX1, a Novel Vacuolar Na+/H+ Antiporter Gene from Solanum torvum, Enhances Salt Tolerance in Transgenic Vegetable Soybean

        Guo-Hu Chen,Wenyan Wang,Li-Fei Yang,Jun-Yi Gai,Yue-Lin Zhu 한국원예학회 2014 Horticulture, Environment, and Biotechnology Vol.55 No.3

        Soil salinity is the primary limiting factor affecting the growth and production of vegetable soybean[Glycine max (L.) Merrill] worldwide. Plants can survive in salinity conditions through the mechanism of sodium ion(Na+) compartmentalization, during which the vacuolar Na+/H+ antiporter (NHX) genes play a critical role. In the presentstudy, a novel vacuolar NHX gene from Solanum torvum Swartz ‘Torvum Vigor’ (StNHX1; GenBank accessionnumber: JN606860.1) was isolated and transferred into the vegetable soybean ‘Xinliaoxian’ through an Agrobacteriummediatedprotocol. The PCR and southern blot analyses confirmed the successful integration of the exogenous genes. The GUS assay and semi-quantitative RT-PCR analysis showed that the alien genes were inherited by the progeniesand that the StNHX1 gene was overexpressed in the T3 generation. The overexpression of this gene conferred hightolerance to salt stress. Under 100 mM NaCl conditions, the scorch scores and the Na+ and malondialdehyde (MDA)contents of the leaves of the transgenic lines were significantly lower as compared with the wild-type (WT) plants,while the K+/Na+ ratio, the contents of K+, relative chlorophyll and relative water, and some morphological traits of thetransgenic plants were significantly higher than those of the WT. These results indicate that the overexpression ofStNHX1 enhances the salt tolerance of vegetable soybean and that StNHX1 is one of the promising target genes thatcan be manipulated to improve the salinity tolerance of crops.

      • KCI등재

        Wnt/β-catenin interacts with the FGF pathway to promote proliferation and regenerative cell proliferation in the zebrafish lateral line neuromast

        Dongmei Tang,Yingzi He,Wenyan Li,Huawei Li 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

        Wnt and FGF are highly conserved signaling pathways found in various organs and have been identified as important regulators of auditory organ development. In this study, we used the zebrafish lateral line system to study the cooperative roles of the Wnt and FGF pathways in regulating progenitor cell proliferation and regenerative cell proliferation. We found that activation of Wnt signaling induced cell proliferation and increased the number of hair cells in both developing and regenerating neuromasts. We further demonstrated that FGF signaling was critically involved in Wnt-regulated proliferation, and inhibition of FGF abolished the Wnt stimulation-mediated effects on cell proliferation, while activating FGF signaling with basic fibroblast growth factor (bFGF) led to a partial rescue of the proliferative failure and hair cell defects in the absence of Wnt activity. Whole-mount in situ hybridization analysis showed that the expression of several FGF pathway genes, including pea3 and fgfr1, was increased in neuromasts after treatment with the Wnt pathway inducer BIO. Interestingly, when SU5402 was used to inhibit FGF signaling, neuromast cells expressed much lower levels of the FGF receptor gene, fgfr1, but produced increased levels of Wnt target genes, including ctnnb1, ctnnb2, and tcf7l2, while bFGF treatment produced no alterations in the expression of those genes, suggesting that fgfr1 might restrict Wnt signaling in neuromasts during proliferation. In summary, our analysis demonstrates that both the Wnt and FGF pathways are tightly integrated to modulate the proliferation of progenitor cells during early neuromast development and regenerative cell proliferation after neomycin-induced injury in the zebrafish neuromast.

      • KCI등재

        A Duplex PCR Assay for Rapid Detection of Phytophthora nicotianae and Thielaviopsis basicola

        Na Liu,Shijun Jiang,Songli Feng,Wenyan Shang,Guozhen Xing,Rui Qiu,Chengjun Li,Shujun Li,Wenming Zheng 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.2

        A duplex PCR method was developed for simultaneous detection and identification of tobacco root rot pathogens Phytophthora nicotianae and Thielaviopsis basicola. The specific primers for P. nicotianae were developed based on its internal transcribed spacer (ITS) regions of ribosomal gene, ras gene and hgd gene, while the specific primers for T. basicola were designed based on its ITS regions and β-tubulin gene. The specificity of the primers was determined using isolates of P. nicotianae, T. basicola and control samples. The results showed that the target pathogens could be detected from diseased tobacco plants by a combination of the specific primers. The sensitivity limitation was 100 fg/μl of pure genomic DNA of the pathogens. This new assay can be applied to screen out target pathogens rapidly and reliably in one PCR and will be an important tool for the identification and precise early prediction of these two destructive diseases of tobacco.

      • SCIESCOPUSKCI등재

        A quantitative method for detecting meat contamination based on specific polypeptides

        Feng, Chaoyan,Xu, Daokun,Liu, Zhen,Hu, Wenyan,Yang, Jun,Li, Chunbao Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.9

        Objective: This study was aimed to establish a quantitative detection method for meat contamination based on specific polypeptides. Methods: Thermally stable peptides with good responses were screened by high resolution liquid chromatography tandem mass spectrometry. Standard curves of specific polypeptide were established by triple quadrupole mass spectrometry. Finally, the adulteration of commercial samples was detected according to the standard curve. Results: Fifteen thermally stable peptides with good responses were screened. The selected specific peptides can be detected stably in raw meat and deep processed meat with the detection limit up to 1% and have a good linear relationship with the corresponding muscle composition. Conclusion: This method can be effectively used for quantitative analysis of commercial samples.

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