Impact of Caspase-8 (CASP8) -652 6N Del and D302H Polymorphisms on Prostate Cancer in Different Ethnic Groups

Zhang, Cheng-Dong,Li, Hong-Tao,Liu, Kun,Lin, Zhi-Di,Peng, Qi-Liu,Qin, Xue,He, Min,Wu, Hua,Mo, Zeng-Nan,Yang, Xiao-Li Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Background: Despite evidence suggesting roles for caspase-8 (CASP8) -652 6N del and D302H polymorphisms in prostate cancer (PCa), the association of these polymorphisms with PCa risk remains inconclusive. Therefore, a meta-analysis was performed to more precisely estimate the association of CASP8 -652 6N del and D302H polymorphisms with PCa susceptibility. Materials and Methods: A comprehensive literature search was conducted to identify all case-control studies of CASP8 D302H and -652 6N del polymorphisms and PCa risk. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the strength of the association and the precision of the estimate, respectively. Results: Nine -625 6N del studies and 4 D302H studies were included. CASP8 -652 6N del and D302H polymorphisms were not significantly associated with PCa risk in the overall analyses. However, in the subgroup analysis stratified by ethnicity, -625 6N del was significantly associated with PCa risk in the East Asian and Indian populations under the recessive model. Furthermore, the subgroup analysis strongly suggested that D302H was associated with lower PCa risk in the Non-Indian population under the dominant model. Conclusions: In our meta-analysis, ethnic-specific differences were evident in the association of CASP8-625 6N del and D302H polymorphisms with PCa risk.

Killing Effects of Different Physical Factors on Extracorporeal HepG2 Human Hepatoma Cells

Zhang, Kun-Song,Zhou, Qi,Wang, Ya-Feng,Liang, Li-Jian Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

Objective: To determine the killing effects on extracorporeal HepG2 cells under different temperatures, pressures of permeability and lengths of treatment time. Method: According to different temperatures, pressures of permeability and lengths of treating time, extracorporeal HepG2 cells of human hepatoma cell-line were grouped to 80 groups. Cell index (CI) as the measurement of killing effect were calculated by monotetrazolium (MTT) methods, i.e., CI =1- (the OD value in treated group - the OD value in blank control group) / (mean of untreated control group - mean of blank control group). According to the factorial design, data were fed into SPSS 10.0 and analyzed by three-way ANOVA (analysis of variance). Result: Temperature, pressure of permeability and length of treating time all had effects on the CI (cell index) level. Length of treating time was the most influential factor of the three. Additionally, any two of them all had statistically significant interactive effects on the CI level. When treated for 5-30 min, destilled water at $46^{\circ}C$ stably generated the highest CI. Conclusion: The "$46^{\circ}C$-destilled water-60 min" was considered as the optimal combination of conditions which lead to highest CI. We suggest exerting celiac lavage for 15 min with stilled water at $40^{\circ}C-43^{\circ}C$ in surgical practice as a hyperthermia treatment to achieve ideal killing effects on free cancer cells, which is feasible, practical, and clinically effective.

Post-Transcriptional Control of Tropoelastin in Aortic Smooth Muscle Cells Affects Aortic Dissection Onset

Qi, You-Fei,Shu, Chang,Xiao, Zhan-Xiang,Luo, Ming-Yao,Fang, Kun,Guo, Yuan-Yuan,Zhang, Wen-Bo,Yue, Jie Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.3

Aortic dissection (AD) is a catastrophic disease with high mortality and morbidity, characterized with fragmentation of elastin and loss of smooth muscle cells. Although AD has been largely attributable to polymorphisms defect in the elastin-coding gene, tropoelastin (TE), other undermined factors also appear to play roles in AD onset. Here, we investigated the effects of post-transcriptional control of TE by microRNAs (miRNAs) on elastin levels in aortic smooth muscle cells (ASMC). We found that miR-144-3p is a miRNA that targets TE mRNA in both human and mouse. Bioinformatics analyses and dual luciferase reporter assay showed that miR-144-3p inhibited protein translation of TE, through binding to the 3'-UTR of the TE mRNA. Interestingly, higher miR-144-3p levels and lower TE were detected in the ASMC obtained from AD patients, compared to those from non-AD controls. In a mouse model for human AD, infusion of adeno-associated viruses (serotype 6) carrying antisense for miR-144-3p (asmiR-144-3p) under CAG promoter significantly reduced the incidence and severity of AD, seemingly through enhancement of TE levels in ASMC. Thus, our data suggest an essential role of miR-144-3p on the pathogenesis of AD.

Inter-domain Alliance Authentication Protocol Based on Blind Signature

Zhang Jie,Zhang Qi-kun,Gan Yong,Yin Yifeng,Tan Yu-an 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.12

In large distributed networks, many computers must be mutual coordination to complete some works under the certain conditions, these computers may come from different domains. For ensuring secure cross domains to access resources among these computers in different domains, this paper proposes a multi-domain union authentication protocol. The protocol adopts blind signature to achieve mutual authentication among entities in different domains. This scheme overcomes the complexity of certificate transmission and the network bottlenecks in the scheme of PKI-based. It can trace the entity identity and supports two-way entities anonymous authentication, which avoid the authority counterfeiting its member to access other domain’s resources. Analysis shows that its advantages on security and communication-consumption.

Radionuclide identification method for NaI low-count gamma-ray spectra using artificial neural network

Sheng Qi,Shanqiang Wang,Ye Chen,Kun Zhang,Xianyun Ai,Jinglun Li,Haijun Fan,Hui Zhao 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.1

An artificial neural network (ANN) that identifies radionuclides from low-count gamma spectra of a NaIscintillator is proposed. The ANN was trained and tested using simulated spectra. 14 target nuclides wereconsidered corresponding to the requisite radionuclide library of a radionuclide identification devicementioned in IEC 62327-2017. The network shows an average identification accuracy of 98.63% on thevalidation dataset, with the gross counts in each spectrum Nc ¼ 100~10000 and the signal to noise ratioSNR ¼ 0.05e1. Most of the false predictions come from nuclides with low branching ratio and/or similardecay energies. If the Nc>1000 and SNR>0.3, which is defined as the minimum identifiable condition, theaveraged identification accuracy is 99.87%. Even when the source and the detector are covered with leadbricks and the response function of the detector thus varies, the ANN which was trained using nonshieldingspectra still shows high accuracy as long as the minimum identifiable condition is satisfied. Among all the considered nuclides, only the identification accuracy of 235U is seriously affected by theshielding. Identification of other nuclides shows high accuracy even the shielding condition is changed,which indicates that the ANN has good generalization performance.

Sanguinarine impairs lysosomal function and induces ROS-dependent mitophagy and apoptosis in human hepatocellular carcinoma cells

Jingjing Wang,Qi Su,Qing Wu,Kun Chen,Asmat Ullah,Mohsin Ahmad Ghauri,Yanmin Zhang 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.11

Hepatocellular carcinoma (HCC) is one of themost common tumor types globally. Despite the progressmade in surgical procedures and therapeutic options, HCCremains a considerable cause of cancer-related mortality. In this study, we investigated the antitumor eff ects of sanguinarine(Sang) on HCC and its potential mechanisms. Our fi ndings showed that Sang impairs the acidic environmentof lysosomes by inhibiting cathepsin D maturation. Inaddition, Sang inhibited the formation of autolysosomes inRFP-GFP-LC3 transfected cells, subsequently suppressinglate mitophagy. Sang also induced reactive oxygen species(ROS)-dependent autophagy and apoptosis in HCC cells,which was signifi cantly attenuated following treatment witha ROS scavenger. Further investigation using autophagyinhibitors revealed that sanguinarine-induced mitochondrialdysfunction and mitophagy led to mitochondrial apoptosisin HCC cells. Immunohistochemical staining of sanguinarine-treated xenograft samples revealed that it initiated andblocked autophagy. In summary, our fi ndings suggest thatin HCC cells, Sang impairs lysosomal function and inducesROS-dependent mitophagy and apoptosis.

Post-Transcriptional Control of Tropoelastin in Aortic Smooth Muscle Cells Affects Aortic Dissection Onset

You-Fei Qi,Chang Shu,Zhan-Xiang Xiao,Ming-Yao Luo,Kun Fang,Yuan-Yuan Guo,Wen-Bo Zhang,Jie Yue 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.3

Aortic dissection (AD) is a catastrophic disease with high mortality and morbidity, characterized with fragmentation of elastin and loss of smooth muscle cells. Although AD has been largely attributable to polymorphisms defect in the elastin-coding gene, tropoelastin (TE), other undermined factors also appear to play roles in AD onset. Here, we investigated the effects of post-transcriptional control of TE by microRNAs (miRNAs) on elastin levels in aortic smooth muscle cells (ASMC). We found that miR-144-3p is a miRNA that targets TE mRNA in both human and mouse. Bioinformatics analyses and dual luciferase reporter assay showed that miR-144-3p inhibited protein translation of TE, through binding to the 3-UTR of the TE mRNA. Interestingly, higher miR-144-3p levels and lower TE were detected in the ASMC obtained from AD patients, compared to those from non-AD controls. In a mouse model for human AD, infusion of adeno-associated viruses (serotype 6) carrying antisense for miR-144-3p (as-miR-144-3p) under CAG promoter significantly reduced the incidence and severity of AD, seemingly through enhancement of TE levels in ASMC. Thus, our data suggest an essential role of miR-144-3p on the pathogenesis of AD.

Three-dimensional Network Structure of Conductive Composites by Hybrid Conductive Fillers of Silver/graphene

Xiao Min Zhang,Kun-yan Wang,Guo Zhao,Qi Long Li,Bin Wang 한국섬유공학회 2019 Fibers and polymers Vol.20 No.6

In order to explore new generations of interconnecting materials in electronic packaging industries, silvernanoparticles (Ag NPS) and graphene nanosheets (GNS) were introduced into matrix resin to prepare conductive composites. The electrical and mechanical of above electrically conductive adhesives (ECAs) were investigated and characterized. It wasfound that the ECAs can be solidified through a chemical sintering in the air at 150 oC for 30 min. The results indicated thatthe percolation threshold of resistivity reaches 3.5×10-4 Ω·cm for ECAs filled with 65 wt.% Ag NPS, 0.5 wt.% GNS and theshear strength reaches 10.8 MPa, suggesting excellent electric conductivity and bonding strength.

Mineralogy and isotope geochemical characteristics for Xiaozhen copper deposit, Langao County, Shaanxi Province and their constraint on genesis of the deposit

Gang Wang,Zong-qi Wang,Rui Shi,Ying-li Zhang,Kun-ming Wang 한국지질과학협의회 2015 Geosciences Journal Vol.19 No.2

The Xiaozhen copper deposit is a typical deposit in the North Daba Mountain area polymetallic metallogenic belt. With the exception of geological prospecting research, however, few studies have been reported on the genesis of the deposit. This paper focuses on the mineralogical and isotope geochemical characteristics for the Xiaozhen copper deposit. Detailed field-work and microscope observations suggest that the ore’s structural fabric and mineral assemblages feature a medium–low-temperature hydrothermal pattern of metallogeny. The results of electron microprobe analysis indicate that chalcopyrite atomic ratio ΣMe/S = ~0.95–1.03, which suggests that they may not be of sedimentary origin. In addition, the S content of the pyrite is from 50.57 wt% to 53.16 wt% that is less than standard the value of 53.45 wt%, the Fe/S value is from 0.878 to 0.955, and the Ni content in pyrite is high even up to 0.38 wt%. These results, combined with Ni-Co graphic projection, indicate that the pyrite is of magmatic hydrothermal origin. Moreover, most of the sphalerites contain Se, and their calculated w(FeS) content ranging from 4.60 wt% to 10.37 wt% suggest magmatic origin and a formation temperature of approximately 300 °C. Further, the content of χ(Fe) is from 46.55 wt% to 46.97 wt% for pyrrhotites, suggesting they are clino-pyrrhotites. This result, combined with mineral assemblages and the Fe-S system diagram, indicates a mineralization temperature of approximately 310 °C. The δ34S values of the sulfide range from 2.6‰ to 11.5‰, indicating multisource properties. The 208Pb/204Pb has wider ratios (~38.897–39.918), reflecting that they contain abundant radiogenic lead. The Pb isotope tectonic setting and genetic classification plot show that the ore lead was provided mainly by the upper crust in addition to a few magma materials. The analytical results of fluid inclusions in the chalcopyrite show that the 3He/4He ratios are ~0.268–0.326 R/Ra, which is higher than the crustal ratios but grossly lower than that of the mantle fluid and that the 40Ar/36Ar ratios are higher than the argon isotopic composition of the atmosphere. Moreover, the He-Ar isotope system suggests that the source of the ore-forming fluids is predominantly meteoric low-temperature underground water, with a small amount of mantle fluid. In summary, this study has determined that the Xiaozhen copper deposit is a medium-temperature magmatic hydrothermal deposit. This finding is combined with metallogenic age to briefly describe the mineralization process.

Decreased Expression of LKB1 Correlates with Poor Prognosis in Hepatocellular Carcinoma Patients Undergoing Hepatectomy

Huang, Yue-Han,Chen, Zhen-Kun,Huang, Ka-Te,Li, Peng,He, Bin,Guo, Xu,Zhong, Jun-Qiao,Zhang, Qi-Yu,Shi, Hong-Qi,Song, Qi-Tong,Yu, Zheng-Ping,Shan, Yun-Feng Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Aim: To study any correlation of LKB1 expression with prognosis in hepatocellular carcinoma (HCC) cases. Methods: A total of 70 HCC patients and 20 primary intrahepatic stone patients in the first affiliated hospital of Wenzhou Medical College were enrolled in this study. LKB1 expression was detected by immunohistochemistry. Patients were followed-up and prognostic factors were evaluated. Result: LKB1 expression was decreased in the HCC samples. Loss of LKB1 expression in HCC was significantly related to histologic grade (P=0.010), vascular invasion (P=0.025) and TMN stage (P=0.011). Patients showing negative LKB1 expression had a significantly shorter disease-free and overall survival than those with positive expression (P = 0.001, P=0.000, respectively). Multivariate Cox regression analysis indicated that LKB1 expression level was an independent factor of survival (P = 0.033). Conclusion: HCC patients with decreased expression LKB1 have a poor prognosis. The loss of LKB1 expression is correlated with a lower survival rate.