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Talin-1 Correlates with Reduced Invasion and Migration in Human Hepatocellular Carcinoma Cells
Fang, Kun-Peng,Zhang, Jian-Lin,Ren, Yan-Hong,Qian, Ye-Ben Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6
Background: Talin-1 is a cytoskeleton protein that participates in cell migration and plays a role in tumor formation, migration, and metastasis in different types of cancer. Chinese investigators have observed that the levels of Talin-1 protein and mRNA expression in HCC tissues are significantly lower than in the adjacent non-cancerous tissue. However, Japanese investigators have reported that Talin-1 is upregulated in HCC. Tln2 as homologous gene of Tln-1, which encodes a very similar protein, but the role of Talin-2 is very little known in primary liver cancer (PLC). We investigated whether the expression of Talin-1 in PLC may be associated with the histological subtype as well as the role of Talin-1 in tumor cell invasion and migration using human hepatocellular carcinoma cell lines. Materials and Methods: We measured the mRNA expression levels of Talin-1 and Talin-2 in five human liver cancer cell lines and normal human liver cell ($LO_2$ cell line) by real-time PCR and the protein expression levels of Talin-1 by Western blot. Migration and invasion of the cells were assessed using transwell assays and cell scratch experiments, respectively, and proliferation was assessed by soft AGAR colony formation. Results: Talin-1 and Talin-2 expression differed significantly between the five human liver cancer cell lines and $LO_2$ cell line (p<0.05). Compared with the $LO_2$ cell line, the invasion and migration capabilities of the five cancer cell lines differed significantly (p<0.05). Similarly, the colony-forming ability differed (p<0.05). Conclusions: High levels of Talin-1 expression are correlated with reduced invasion and migration as well as decreased malignancy in human liver cancer cell lines; the suppression of Talin-1 promotes invasion and migration. In addition, Talin-2 may be correlated with invasion and migration in human hepatocellular carcinoma.
Kun Fang,Xu Ye,Yawen Huang,Guanjun Chang,Junxiao Yang,Yueqin Shen 한국고분자학회 2015 Macromolecular Research Vol.23 No.6
Three original conjugated polymers were devised and synthesized via the alternating conjugated polymerization of the electron-donating monomer of the modified benzo[1,2-b:4,5-b']dithiophene (BDT) and electronaccepting monomers of perylene diimide (PDI), dithienyl-perylene diimide (DTPDI) and dithienocoronene diimide (DTCDI), respectively. The synthesized conjugated polymers presented excellent thermal stability (Td >400 oC) as well as broad absorption in visible region and narrow gap of energy level (1.80 to 2.01 eV). Moreover, P(BDT-DTCDI) exhibited strong fluorescence properties ( =736 nm with excitation at 680 nm). According to the theoretical calculations, P(BDT-PDI) and P(BDT-DTPDI) displayed significant polymer backbone torsion (41.44o and 39.64o), and P(BDT-DTCDI) had highly coplanar backbone with negligible angle between BDT and DTCDI. The results indicated that P(BDT-DTCDI) had potential applications in the fields of organic thin-film transistors (OTFTs) and fluorescence materials, and P(BDT-PDI) and P(BDT-DTPDI) could be used as a stable n-type semiconductor for polymer solar cells.
You-Kun Zheng,Cui-Ping Miao,Hua-Hong Chen,Fang-Fang Huang,Yu-Mei Xia,You-Wei Chen,Li-Xing Zhao 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.3
Background: Endophytic fungi play an important role in balancing the ecosystem and boosting host growth. In the present study, we investigated the endophytic fungal diversity of healthy Panax notoginseng and evaluated its potential antimicrobial activity against five major phytopathogens causing rootrot of P. notoginseng. Methods: A culture-dependent technique, combining morphological and molecular methods, was used to analyze endophytic fungal diversity. A double-layer agar technique was used to challenge the phytopathogens of P. notoginseng. Results: A total of 89 fungi were obtained from the roots, stems, leaves, and seeds of P. notoginseng, and 41 isolates representing different morphotypes were selected for taxonomic characterization. The fungal isolates belonged to Ascomycota (96.6%) and Zygomycota (3.4%). All isolates were classified to 23 genera and an unknown taxon belonging to Sordariomycetes. The number of isolates obtained from different tissues ranged from 12 to 42 for leaves and roots, respectively. The selected endophytic fungal isolates were challenged by the root-rot pathogens Alternaria panax, Fusarium oxysporum, Fusarium solani, Phoma herbarum, and Mycocentrospora acerina. Twenty-six of the 41 isolates (63.4%) exhibited activity against at least one of the pathogens tested. Conclusion: Our results suggested that P. notoginseng harbors diversified endophytic fungi that would provide a basis for the identification of new bioactive compounds, and for effective biocontrol of notoginseng root rot.