上顎洞에 發生한 Aspergillosis의 一例報告

林大銑,宋圭鉉,朴敬浩,朴暎緖,李玩永 中央醫學社 1963 中央醫學 Vol.5 No.4

A case of secondary aspergillosis of the left maxillary sinus following chronic sinusitis in a 42 year old male coal miner specialist is reported. His chief complaints were nasal obstruction and postnasal drip of several years duration. Roentgenogram revealed marked and moderate shadow in the left and right maxillary sinuses respectively. Significant operative findings included almost paper-thin bony walls of the left maxillary sinus, and small amount of yellow greenish purulent material was drained, followed by yellowish brown semisolid tissue on further curettage. The latter specimen was found to be essentially mass of fungi, showing numerous distinctly septate, branching hyphae on the Papanicolaou stain of the direct smears of the cut surface of the specimen, and Hematoxylin-Eosin and Periodic Acid Schiff stains of paraffin sections. Pertinent aspects of clinical and pathological differential diagnosis between aspergillosis - and mucormycosis, are described. Aspergillosis presents usually benign course, with insignificant chief complaints as our case, whereas mucormycosis is most frequently associated with uncontrollable diabetics, and run usually acutely fatal course. There are three main types of ucormycosis-cerebral, pulmonary and intestinal. The characteristic broad nonseptate branching hyphae of mucor, and marked affinity to the blood vessels,, penetrating the tough walls of arteries, with thromboses and infarction, readily differentiate aspergillosis from mucormycosis.

상악 절치부-토크에 의한 치아 이동과 응력 분포에 관한 유한요소법적 연구

윤현주,임용규,이동렬,조영수 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.2

본 연구는 상악 절치부에 active 토크가 가해졌을 경우, 교정력을 직접 받은 치아와 인접 치아의 반응을 알아보고자 상악 치아 및 치조골의 유한요소 모델을 제작하고, stainless steel, NiTi, TMA 세 종류의 각형 호선을 육면체 요소로 모델링하여 유한요소 모델을 완성하였다. 호선이 브라켓에 삽입되었을 때 브라켓에 발생하는 반력과 모멘트를 구하였고, 이것을 유한 요소 모델에 적용하여 각 치아의 변위와 응력 분포를 측정하였다. 브라켓에 발생하는 반력은 근원심 방향과 협설 방향으로의 힘은 0에 가까우며, 중절치, 측절치, 견치의 브라켓 근심측에서는 정출력이 원심측에서는 압하력이 발생하였다. 힘과 모멘트의 크기는 측절치에서 최대였고, 중절치, 견치 순으로 감소하였고, 소구치 부위와 대구치 부위에서는 급격히 감소하였다. 중절치와 측절치는 치관 협측, 원심 경사 이동과 압하를 보였으며, 견치는 치관 설측 원심 경사와 정출을 보였고, 제1소구치는 치관 설측 경사이동을 보였다. .019×.025 SS을 사용하여 상악 절치부에 토크를 부여하는 경우에는 측절치에 과도한 힘이 집중되므로, 임상에서 토크를 조절할 경우에는 NiTi나 TMA 호선을 사용하는 것이 바람직하리라고 생각된다. The purpose of this study was to investifate the stress distribtion in the periodontal tissue and the displacement of teeth when active torque was applied to the maxillary incisors by three-dimen-sional finite element analysis. A three- dimensional finite element model consisted of the maxillary teeth and surrounding periodontal membrane, .022×.028 Roth prescription bracket and stainless steel. NiTi and TMA rectangular ideal arch wires which were modeled by hexahedron elements. Applied active torques were 2, 5 and 10 degrees. The findings of this study showed that the reaction force acting on the bracket was the extrusion force on the mesial side of the incisors and canine and the intrusion force on the distal side of the incisors and canine.The amount of force and moment was greatest at the lateral incisor. When active anterior labial crown torque was applied, labial crown and distal tipping and intrusion of the incisors took place, and lingual crown distal tipping and extrusion of the canin occured. An excessive force was concentrated on the lateral incisor, when the stainless steel wire was used. NiTi or TMA wire os desirable for torque control.

Novel dihydrobenzofuro[4,5-b][1,8]naphthyridin-6-one derivative, MHY-449, induces cell cycle arrest and apoptosis via the downregulation of Akt in human lung cancer cells

LIM, HYUN SOOK,KANG, YONG JUNG,SUNG, BOKYUNG,KIM, SEON HEE,KIM, MIN JEONG,KIM, HYE RIM,KIM, SEONG JIN,CHOI, YUNG HYUN,MOON, HYUNG RYONG,CHUNG, HAE YOUNG,KIM, NAM DEUK Spandidos Publications 2015 Oncology reports Vol.34 No.5

<P>The anticancer properties of MHY-449, a novel dihydrobenzofuro [4,5-b] [1,8]naphthyridin-6-one derivative, in various human cancer cell lines have been previously reported. The aim of the present study was to investigate the activities of MHY-449 on human lung cancer cells in order to elucidate its underlying molecular mechanisms of action. The result showed that MHY-449 treatment inhibited cell growth in a time- and concentration-dependent manner. Specifically, MHY-449 induced cell cycle arrest at the S phase, and the resulting increased sub-G1 fraction led to the induction of apoptosis, as determined by flow cytometric analysis and DNA fragmentation. In addition, MHY-449 was shown to induce alterations in the ratio of Bax/Bcl-2 protein expression, and contribute to the loss of mitochondrial membrane potential. These cellular events then triggered the caspase cascade and subsequent poly(ADP-ribose) polymerase cleavage. The apoptotic cell death induced by MHY-449 was inhibited by pretreatment with Z-VAD-FMK, a pan-caspase inhibitor. Moreover, MHY-449 downregulated the phosphorylation of Akt, and the phosphatidylinositol-3 kinase/Akt inhibitor LY294002 was found to enhance its induction of apoptosis. Taken together, the results suggested that MHY-449 exerts anticancer effects by promoting cell cycle arrest and apoptosis via the downregulation of Akt. Based on these data, MHY-449 serves as a potential candidate in the chemoprevention and/or treatment of lung cancer.</P>

Clinical and Pathologic Features of Elderly Gastric Cancer

( Joo Hyun Lim ),( Dong Ho Lee ),( Cheol Min Shin ),( Na Yung Kim ),( Young Soo Park ),( Hyun Chae Jung ),( 1in Sung Song ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.1

Background: Gastric cancer is one of the most common cancers in Korea and the incidence of which is great in elderly patients. However, not much is known about elderly gastric cancer. The aim of this study was to evaluate the specific features of gastric cancer in elderly patients. Methods: We reviewed medical records of 1107 patients who had undergone radical gastrectomy for gastric cancers between June 2005 and December 2009. All the patients were divided into two groups: Non-elderly group aged under 65 (n=676) and elderly group aged 65 and above (n=431). Results: There were more symptomatic patients among elderly group compared with non-elderly group (54.2% vs. 62.1%; p=0.009). Comorbidity was more prevalent in the elderly group (29.6% vs. 61.9%; p<0.001). The elderly group showed higher frequency of elevated tumor markers (CEA: 4.9% vs. 8.7%; p=0.041), CA19-9: 6.1% vs. 13.2%; p=0.001) and advanced diseases (42.5% vs. 49.4%, p=0.023).There were no significant differences in N stage (62.4% vs. 58.2%; p=0.164) or H. pylori status (63.6% vs. 57.5%; p=0.153) between the two groups. More cancers were located in lower third among the elderly group (45.1% vs 51.3%; p=0.045). The elderly group had more patients with Lauren intestinal type (40.7% vs. 58.2%; p<0.001) and well (8.0% vs. 13.7%; p=0.002) or moderately differentiated adenocarcinoma (29.6% vs. 44.1%; p<0.001). Rate of complication did not differ between the two groups (6.1% vs. 8.1%; p=0.187). Microsatellite instability (p<0.001) and p53 overexpression (p<0.001) were more frequent in the elderly group. Conclusion: Elderly gastric cancer showed a tendency of being diagnosed at more advanced stage with symptoms. However, it was related to pathologic features known for favorable prognosis except for p53 overexpression. Also surgery in elderly was as tolerable as in young patients. Thus we recommend to consider surgery more frequently for elderly patients with operable gastric cancers.

통계청 근무자를 위한 한국판 직무스트레스 척도의 개발

윤현주(Hyun Ju Yun),윤보현(Bo-Hyun Yoon),정유란(Yu Ran Jeong),임형문(Hyung-Moon Lim),백수정(Soo Jung Baek),오수정(Soo Jung Oh),조성덕(Sung-Duk Jo),최고은(Ko-Eun Choi),정숙인(Suk-In Jung),강다영(Da Yung Kang),시영화(Yung-Hwa Sea),송제헌( 대한사회정신의학회 2016 사회정신의학 Vol.21 No.2

목 적 : 본 연구는 통계청 근무자의 직무스트레스를 효율적으로 관리하고 정신건강을 증진하기 위해 통계청의 직무에 특이적인 스트레스 척도를 개발하고 개발된 척도의 신뢰도와 타당도를 검증하였다. 방 법 : 호남 지역 통계청에 근무하는 남·여 480명을 대상으로 하였다. 한국형 직무스트레스 척도를 기본으로 하고, 예비연구를 통해 문항이 새롭게 보강된 ‘통계청 근무자를 위한 한국판 직무스트레스 척도’(이하 통계청 직무스트레스 척도)를 개발하였으며, 이를 단축형 일반건강척도(GHQ-12), 간이정신진단검사(SCL-90-R), 삶의 만족도 척도(SWLS)를 이용하여 비교하였다. 통계청 직무스트레스 척도 문항에 대한 내적 일치도, 탐색적 요인분석 및 정신건강관련 척도와의 상관분석을 시행하였다. 모든 분석은 SPSS 21.0을이용하였다. 결 과 : 개발된 척도의 문항 내적 일치도는 우수한 편이었다(Cronbach’s alpha=0.56-0.79). 통계청 직무스트레스 척도의 요인분석 결과 직무특성 및 감정노동, 직무적성 및 만족, 조직문화, 상사리더십, 조직 내 관계, 업무부담, 공정한 대우, 급여 및 복지 등 8개의 요인이 선택되었다. 최종적으로 구성된 통계청 직무스트레스 척도와 정신건강관련 척도와의 상관관계는 모두 통계적으로 유의하였다 (p<0.05). 결 론 : 본 연구에서 개발된 통계청 직무스트레스 척도는 통계청 업무의 특성을 반영하고 있어 통계청 근무자의 직무스트레스 평가 및정신건강 증진에 유용할 것이다. Objectives : The aim of this study is to develop the Korean Occupational Stress Scale for the National Statistics Office Workers (KOSS-NSOW) and to examine the reliability and validity of this job-specific stress scale. Methods : A total of 480 subjects who were working at the Honam National Statistics Office were included. They completed the preliminary KOSS-NSOW, the 12-item general health Questionnaire (GHQ-12), Symptom checklist-90-Revision (SCL-90-R), Satisfaction with the life scale (SWLS). The Data were analyzed for internal consistency, factor structure and correlations using SPSS 21.0 (p<0.05). Results : KOSS-NSOW’s internal consistency using Cronbach’s alpha was superior (Cronbach’s alpha=0.56-0.79). The factor analysis revealed eight-factors: Occupational characteristics and emotional labor, Occupational aptitude and satisfaction, Organizational culture, Leadership of superior, Relationship in organization, Workload, Fair treatment, Salary and welfare were. A good validity was clearly shown by significant correlations with KOSS-NSOW and GHQ-12, SCL-90-R, SWLS (p<0.05). Conclusion : This newly developed KOSS-NSOW might be useful instrument for the evaluation of occupational stress in the statistics workers and for improving mental health.

울혈성 심부전 환자의 치료 경과중 혈청 마그네슘의 동태에 관한 고찰

임종훈(Jong Hoon Lim),전국진(Kook Jin Chun),정준훈(Joon Hoon Jeong),김병진(Byung Jin Kim),오현명(Hyun Myung Oah),박용현(Yong Hyun Park),박융인(Yong Hyun Park),홍택종(Taek Jong Hong),신영우(Yung Woo Shin) 대한내과학회 1997 대한내과학회지 Vol.53 No.6

N/A Objectives: There are many interesting reports suggesting that magnesium(Mg) deficiency is deleterious in patients with congestive heart failure (CHF). It is paradoxical that the most important cause of Mg deficiency in these persons is maybe use of therapeutics including diuretics. Authors investigated the trend of serum and 24 hour urine Mg with other relating electrolytes in Mg homeostasis prospectively, in the management of CHF. And we assessd the effects of medications and many variables in .CHF on serum Mg, and the usefulness of serum Mg representing the body content. Methods: Fifty three patients who were diagnosed as CHF by clinical finding and echocardiogaphy were prescribed conventional doses of diuretics as furosemide 40mg and spironolactone 50mg daily, with or without angiotensin converting enzyme(ACE) inhibitor and digitalis. And then, serial serum and 24 hour urine Mg, sodium, potassium and calcium were obtained at admission, 2nd day, 5th day, and discharge. Results: The patients group with chronic CHF, which was defined as long-term use of diuretics over 6 months, showed higher prevalence of low level of serum Mg concentration than the group with acute one(11 of 28, 39% vs. 2 of 25. 8%, P< 0.01). Of those two groups, the latter showed upward trend of serum Mg from admission to discharge, but the former showed no change. In 24 hour urine Mg excretion, the amount of the patients with CHF was larger than that of control group. In the chronic CHF group, the effect of digitalis on decreasing serum Mg was evident. Serum Mg of acute CHF group correlated with serum BUN(r=0.5609). Whereas, that of chronic group with ejection fraction(r= ?0.4742) and plasma renin activity(r= ? 0.3791), with serum potassium(r=0.4673) and creatinine(0.5846). Serum Mg may be useful indicator of Mg homeostasis, especially in chronic CHF patients. Conclusion: Because patients with chronic CHF were prone to deficiency of Mg in the management, maintaining the adequate serum Mg through long- term replacement seems very important in decreasing the morbidity and mortality of these persons.

Drosohila melanogaster의 (w^i)_4계를 이용한 체세포 유전자 돌연변이 유발에 관한 연구

임영미,최영현,김종숙,유미애,이원호 부산대학교 유전공학연구소 1994 분자생물학 연구보 Vol.10 No.-

새로운 체세포 눈색 돌연변이 검출계인 Drosophila meanogaster의 (w^1)₄계를 이용하여 돌연변이원으로 알려진 IQ, Trp-P-2,EMS 및 MMc의 체세포 유전자 돌연변이원성과 killing effect를 비교 조사함으로써, (w^1)₄계가 감수성이 높은 환경성 돌연변이원 및 발암원 검출계로서 사용할수 있는지의 여부를 조사한 결과는 다음과 같다. (w^1)₄계에서 대조구의 체세포 유전자 돌연변이 유발빈도는 약 1.06%로 나타났으며, 조사된 4가지 종류의 돌연변이원들에 대하여 모두 양성 반응을 나타내었다. 체세포 돌연변이원성을 조사한 농도에서 각 변이원들의 killing effect여부를 생존도로 조사하였는데, IQ 0.3㎎/㎖ 이외의 모든 처리구에서killing effect는 없었다. 이는 본 실험에서 유발된 돌연변이 빈도로서 각 변이원의 돌연변이원성의 정도를 비교할 수 있음을 의미한다. (w^1)₄계에서 같은 heterocyclic amines 이면서도 Trp-P-2가 IQ보다 돌연변이원서이 높은 것으로 나타났으며, 같은 alkylating agents 이지만 MMC가 EMS보다 약한 돌연변이원성을 나타낸다는 것을 알 수 있었다. 각 돌연변이원들의 돌연변이 유발빈도를 다른 검출계에서의 결과와 비교해 본 결과, (w^1)₄계가 종전의 uz계나 w^1계어서 보다 높은 유발빈도를 나타내어 훨씬 적은 개체수로서 환경성 돌연변이원 및 발암원을 검출할 수 있는 감수성이 높은 계임을 확인할 수 있었다. In Drosophila meanogaster, somatic gene mutagenicity and killing effect of IQ, Trp-P-2,EMS and MMc known as positive mutagens were examined by using(w^1)₄, a new eye-color mutation assaying system. By analysis of these results, whether (w^1)₄system can be used as a sentive system for enviornmental mutagens and carcinogens was investigated. In (w^1)₄system, the frequency of spontaneous somatic gene mutation was nearly 1.06%, and (w^1)₄system was showed positive response to mutagenicity in all thr groups treated with IQ, Trp-P-2,EMS and MMc. In the concentrations which examined somtis mutagenicity, the killing effects of each mutagene was surveyed by its viability. In all the reated groups, the killing effects were not appeared eacept the 0.3㎎/㎖ group treated with IQ. This suggests that the powerfulness of mutagenicity of each mutagen may be compared with induced mutation frequency. On thouse groups, it could know that Trp-P-2 was higher than IQ in the mutagenicity, though the same heterocyclic amine, and MMC was more or less lower than EMS in that , though the same alkylating agents. In comparing mutation frequency of each mutagen, (w^1)₄system was occured higher frequency tham the other eye-color mutation assaying systems(uz and w^1 system). Hence, it was confirmed that (w^1)₄system was the sentivie assaying system, which could detect enviornmental mutagens and carcinogens by way of evaluating even fewer the number of flies.

Vaccination with an Ovalbumin/lnterleukin-4 Fusion DNA Efficiently Induces Th2 Cell-Mediated Immune Responses in an Ovalbumin-Specific Manner

Lim, Young Shin,Kang, Bok Yun,Kim, Eui Jin,Kim, Seung Hyun,Hwang, Seung Yung,Kim, Kyeong Man,Kim, Tae Sung 전남대학교 약품개발연구소 1998 약품개발연구지 Vol.7 No.1

To more effectively drive immune responses toward antigen-specific T helper type 2 (Th2) cell-mediated responses, we constructed a mammalian expression vector (pOVA/IL4) carrying a fused gene in which the ovalbumin (OVA) cDNA was covalently linked to murine interleukin-4 (IL-4) cDNA. A biologically active OVA/IL4 protein was expressed by the transfected COS cells with the pOVA/IL4 DNA, as demonstrated by Western blotting and cytokine bioassay. Intramuscular injection of BALB/c mice with the pOVA/IL4 DNA increased both the production of OVA-specific IL-4 by CD4^+ T cells and the ratio of anti-OVA IgG1 to anti-OVA IgG2a isotypes, while the injection with the pOVA DNA alone, or with the mixture of the pOVA and pIL4 DNA did no or little increase. Furthermore, the OVA-specific, Th2 cell-mediated immune responses were significantly enhanced by multiple injections with the pOVA/IL4 DNA. These studies indicate that the direct linkage of an OVA gene to an IL-4 gene in the expression plasmid confines the effects of IL-4 to the OVA-specific cells, efficiently driving the immune response toward OVA-specific, Th2 cell-mediated responses.

Poster Session : Bovine Lactoferrin suppresses T cell activation induced by concanavalin A

( Yung Choon Yoo ),( Jung Lim Lee ),( Tae Hyun Paik ) 한국생화학분자생물학회 (구 한국생화학회) 2006 생화학분자생물학회 춘계학술발표논문집 Vol.2006 No.-

S-368 : Clinical implication of crescentic lesions in immunoglobulin A nephropathy

( Yung Ly Kim ),( Mi Jung Lee ),( Ki Heon Nam ),( Kyoung Sook Park ),( Young Eun Kwon ),( Seung Yeong Ahn ),( Hyung Jung Oh ),( Tae Hyun Yoo ),( Shin Wook Kang ),( Beom Jin Lim ),( Hyeon Joo Jeong ),( 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Background and objectives: To date, there has been much controversy about the role of crescentic lesion as a significant prognostic factor in immunoglobulin A nephropathy (IgAN). This study, therefore, evaluated whether crescentic lesions predict adverse renal outcomes in patients with IgAN. Design, setting, participants, and measurements: A total of 430 patients with biopsy-proven IgAN between January 2001 and December 2010 were included. Histologic variables of the Oxford classification (Oxford-MEST) and the presence of crescents were assessed. The primary endpoint was a 50% decline in estimated glomerular filtration rate. Results: Of the 430 patients, 81 (18.8%) patients had a crescentic lesion. During a mean follow-up of 61 months, the primary outcome occurred in 19 (23.5%) patients with crescents compared to 40 (11.5%) patients without crescents (p=0.01). A Kaplan-Meier plot showed that the 10-year renal survival rate was significantly lower in patients with crescents than patients without crescents (p=0.01). However, in a multivariable Cox analysis which included clinical factors and the Oxford-MEST, crescents were not significantly associated with an increased risk of developing the primary outcome [hazard ratio: 0.78, 95% confidence interval (CI): 0.40-1.51, p=0.48]. Furthermore, adding crescents to the Oxford classification did not improve the discriminative ability for the prediction of renal outcomes [c-statistic: 0.86 (0.81-0.91) vs. 0.86 (0.80-0.91), p=0.35]. Conclusions: This study showed that crescentic lesion was not an independent prognostic factor, suggesting that crescents have limited value in predicting renal outcomes of IgAN.