Expression of three neuropeptide genes (SfEH, SfETH, and SfCCAP) in response to insecticide stress on the white-backed planthopper Sogatella furcifera (Hemiptera: Delphacidae)

Long Gui-yun,Wang Zhao,Yang Jia-peng,Tang Chen-hong,Jin Dao-Chao,Yang Hong,Zhou Cao,Yang Xi-bin 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.3

Insect neuropeptides are involved in many important developmental processes. Here, we cloned three neuro peptide genes (SfEH, SfETH, and SfCCAP) from Sogatella furcifera (Horváth) and analyzed the relative expression levels of these genes through Real-time Quantitative polymerase chain reaction (RT-qPCR) during different developmental stages and in different wing types of S. furcifera following exposure to sublethal concentrations (LC 10 and LC 25 ) of methylaminoavermectin benzoate. The results revealed that the three neuropeptide genes (SfEH, SfETH, and SfCCAP) of S. furcifera contained open reading frame sequences of 222, 636, and 408 bp, encoding 73, 211, and 135 amino acids, respectively. Moreover, the expression of SfEH and SfETH reached its peak on day 3 of the 5 th instar nymph stage, while the SfCCAP expressed pinnacle on 40 min after eclosion. Furthermore, the relative expression levels of the three genes were in the following order: SfEH, short-winged female > long-winged female > long-winged male; SfETH, long-winged female > short-winged female > longwinged male; and SfCCAP, short-winged female > long-winged male > short-winged female. Upon calculating the relative expression of the three peptide genes after 24 and 48 h of treatment with sublethal concentrations of methylaminoavermectin benzoate, it was found that the relative mRNA expression levels of SfEH and SfETH declined upon LC 10 treatment but increased upon LC 25 treatment. Meanwhile, the relative mRNA expression level of SfCCAP declined upon both LC 10 and LC 25 treatments. This result will help us understand the effects of pesticides on insect growth and development at the molecular level.

A modified heteropoly acid catalyst with cetyltrimethylammonium bromide for methacrolein to methacrylic acid

Yun-Li Cao,Lei Wang,Li-Long Zhou,Bao-Hua Xu,Yan-Yan Diao,Suojiang Zhang 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.65 No.-

A novel type of heteropolyacids, CTAB-CsH3PMo11VO40 (CTA-CsPAV), was developed by a method of emulsion synthesis using cetyltrimethyl ammonium bromide (CTAB) as the structure promoter, which was applied as a catalyst in the oxidation of methacrolein (MAL) to methacrylic acid (MAA) with fixed-bed reactor. The structure and property of CTA-CsPAV were characterized by FT-IR, XRD, nitrogen adsorption-desorption, NH3-TPD, SEM, XPS, and so on. The structure-performance relationship of CTA-CsPAV was systematically studied, wherein the influence of surface area, acidity, redox property of CTA-CsPAV were focused and discussed. Importantly, the long-term performance of CTA-CsPAV was also investigated.

Prevalence and Genotype Distribution of Human Papillomavirus among Women from Henan, China

Wang, Xiao-Chuan,Sun, Liang-Qi,Ma, Li,Li, Hua-Xin,Wang, Xiu-Li,Wang, Xin,Yun, Tian,Meng, Nian-Long,Lv, Da-Le Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Human papillomavirus (HPV) infection has been implicated as a causative of cervical cancer. In the present study, a total of 578 samples from females attending the gynecological outpatient clinic in Henan province, China, were collected and the HPV genotypes were detected by gene chip and flow-through hybridization. Overall, 44.5% (257/578) females were found to be HPV DNA positive, and the high risk HPV (HR-HPV) rate was 35.1% (203/578). The first peak of HR-HPV infection appeared in the >60 year-old group (55.0%), and the second was within the 51-55 year-old group (50.0%) (${\chi}^2$=19.497, p<0.05). HPV 16 was the most prevalent genotype (9.2%), followed by HPV 52 (7.8%), HPV 6 (6.9%), HPV 11 (5.9%) and HPV 42 (5.0%). The single type HPV infection was 30.4%, with the five majority prevalent genotype HPV 16 (16.5%), HPV 52 (14.3%), HPV 6 (12.6%), HPV 42 (8.6%), HPV 31 (5.1%). The multiple-type HPV infections were 14.0%, and HPV 16 was the most prevalent type (29.6%), followed by HPV 52 (24.7%), HPV 6 (22.2%), HPV 11 (22.2%), HPV 42 (17.3%) and HPV 39 (17.3%).

Pan-cancer Analysis of Tumor Mutational Burden and Homologous Recombination DNA Damage Repair Using Targeted Next-Generation Sequencing

Hai-Yun Wang,Ling Deng,Ying-Qing Li,Xiao Zhang,Ya-Kang Long,Xu Zhang,Yan-Fen Feng,Yuan He,Tao Tang,Xin-Hua Yang,Fang Wang 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4

Purpose Current variability in methods for tumor mutational burden (TMB) estimation and reporting demonstrates the urgent need for a homogeneous TMB assessment approach. Here, we compared TMB distributions in different cancer types using two customized targeted panels commonly used in clinical practice. Materials and Methods TMB spectra of 295- and 1021-gene panels in multiple cancer types were compared using targeted next-generation sequencing (NGS). The TMB distributions across a diverse cohort of 2,332 cancer cases were then investigated for their associations with clinical features. Treatment response data were collected for 222 patients who received immune-checkpoint inhibitors (ICIs) and their homologous recombination DNA damage repair (HR-DDR) and programmed death-ligand 1 (PD-L1) expression were additionally assessed and compared with the TMB and response rate. Results The median TMB between gene panels was similar despite a wide range in TMB values. The highest TMB was eight and 10 in patients with squamous cell carcinoma and esophageal carcinoma according to the classification of histopathology and cancer types, respectively. Twenty-three out of 103 patients (22.3%) were HR-DDR–positive and could benefit from ICI therapy; out of those 23 patients, seven patients had high TMB (p=0.004). Additionally, PD-L1 expression was not associated with TMB or treatment response among patients receiving ICIs. Conclusion Targeted NGS assays demonstrated the ability to evaluate TMB in pan-cancer samples as a tool to predict response to ICIs. In addition, TMB integrated with HR-DDR–positive status could be a significant biomarker for predicting ICI response in patients.

Heavy concrete shielding properties for carbon therapy

Jin-Long Wang,Jiade J Lu,Da-Jun Ding,Wen-Hua Jiang,Ya-Dong Li,Rui Qiu,Hui Zhang,Xiao-Zhong Wang,Huo-Sheng Ruan,Yan-Bing Teng,Xiao-Guang Wu,Yun Zheng,Zi-Hao Zhao,Kai-Zhong Liao,Huan-Cheng Mai,Xiao-Dong Korean Nuclear Society 2023 Nuclear Engineering and Technology Vol.55 No.6

As medical facilities are usually built at urban areas, special concrete aggregates and evaluation methods are needed to optimize the design of concrete walls by balancing density, thickness, material composition, cost, and other factors. Carbon treatment rooms require a high radiation shielding requirement, as the neutron yield from carbon therapy is much higher than the neutron yield of protons. In this case study, the maximum carbon energy is 430 MeV/u and the maximum current is 0.27 nA from a hybrid particle therapy system. Hospital or facility construction should consider this requirement to design a special heavy concrete. In this work, magnetite is adopted as the major aggregate. Density is determined mainly by the major aggregate content of magnetite, and a heavy concrete test block was constructed for structural tests. The compressive strength is 35.7 MPa. The density ranges from 3.65 g/cm³ to 4.14 g/cm³, and the iron mass content ranges from 53.78% to 60.38% from the 12 cored sample measurements. It was found that there is a linear relationship between density and iron content, and mixing impurities should be the major reason leading to the nonuniform element and density distribution. The effect of this nonuniformity on radiation shielding properties for a carbon treatment room is investigated by three groups of Monte Carlo simulations. Higher density dominates to reduce shielding thickness. However, a higher content of high-Z elements will weaken the shielding strength, especially at a lower dose rate threshold and vice versa. The weakened side effect of a high iron content on the shielding property is obvious at 2.5 µSv=h. Therefore, we should not blindly pursue high Z content in engineering. If the thickness is constrained to 2 m, then the density can be reduced to 3.3 g/cm³, which will save cost by reducing the magnetite composition with 50.44% iron content. If a higher density of 3.9 g/cm³ with 57.65% iron content is selected for construction, then the thickness of the wall can be reduced to 174.2 cm, which will save space for equipment installation.

Adaptive Second-order Sliding Mode Control of Electrical Throttles Based on Online Zero-crossing Checking

Yun Long,Chong Yao,En-Zhe Song,Quan Dong,Yan-Min Wang 제어·로봇·시스템학회 2024 International Journal of Control, Automation, and Vol.22 No.2

In this paper, an adaptive second-order sliding mode control approach is proposed for the performance improvement of electronic throttles (ET). Based on the traditional twisting approach, a novel adaptation mechanism based on the online zero-crossing checking is contained in the modified approach to make the control magnitude of the controller at the minimum admissible level. The idea behind it is to calculate the number of zero-crossings of the sliding surface in real time. The guaranteed stability condition and convergence region of the system are also deduced. In order to further prove its high adaptation capability, the commonly used adaptation mechanism called the Lyapunov-based type is also introduced for comparative study. Simulations and experiments validate the proposed approach with the advantages of chattering elimination, high speed and accuracy in the control of ET systems.

A cavitation performance prediction method for pumps PART1-Proposal and feasibility

Yun, Long,Rongsheng, Zhu,Dezhong, Wang Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.11

Pumps are essential machinery in the various industries. With the development of high-speed and large-scale pumps, especially high energy density, high requirements have been imposed on the vibration and noise performance of pumps, and cavitation is an important source of vibration and noise excitation in pumps, so it is necessary to improve pumps cavitation performance. The modern pump optimization design method mainly adopts parameterization and artificial intelligence coupling optimization, which requires direct correlation between geometric parameters and pump performance. The existing cavitation performance calculation method is difficult to be integrated into multi-objective automatic coupling optimization. Therefore, a fast prediction method for pump cavitation performance is urgently needed. This paper proposes a novel cavitation prediction method based on impeller pressure isosurface at single-phase media. When the cavitation occurs, the area of pressure isosurface S_iso increases linearly with the NPSH_a decrease. This demonstrates that with the development of cavitation, the variation law of the head with the NPSH_a and the variation law of the head with the area of pressure isosurface are consistent. Therefore, the area of pressure isosurface S_iso can be used to predict cavitation performance. For a certain impeller blade, since the area ratio R_s is proportional to the area of pressure isosurface S_iso, the cavitation performance can be predicted by the R_s. In this paper, a new cavitation performance prediction method is proposed, and the feasibility of this method is demonstrated in combination with experiments, which will greatly accelerate the pump hydraulic optimization design.

SUNSPOT MOTIONS ASSOCIATED WITH THE 3B/X1.5 SOLAR FLARE OF 13 MAY 1981

WANG JIA LONG,ZHANG GUIQING,MA GUANYI,YUN HONG SIK The Korean Astronomical Society 1996 Journal of The Korean Astronomical Society Vol.29 No.2

We have examined morphological change and movements of individual sunspots within a sunspot group in association with a large solar flare activity (3B/X1.5) appeared on 13 May 1981. For this purpose we measured distance among spots during the period before and after the flare activity and estimated the average velocity of their movement. Our main results are as follows: (1) The longitudinal displacement among sunspots are generally greater than the latitudinal displacement. (2) During the period the spots moved with an average velocity of 1.2 km/s in longitude and 0.86 km/s in latitude. (3) The most notable change took place in the central part placed between the two ribbons of the flare.

A cavitation performance prediction method for pumps: Part2-sensitivity and accuracy

Long, Yun,Zhang, Yan,Chen, Jianping,Zhu, Rongsheng,Wang, Dezhong Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.11

At present, in the case of pump fast optimization, there is a problem of rapid, accurate and effective prediction of cavitation performance. In "A Cavitation Performance Prediction Method for Pumps PART1-Proposal and Feasibility" [1], a new cavitation performance prediction method is proposed, and the feasibility of this method is demonstrated in combination with experiments of a mixed flow pump. However, whether this method is applicable to vane pumps with different specific speeds and whether the prediction results of this method are accurate is still worthy of further study. Combined with the experimental results, the research evaluates the sensitivity and accuracy at different flow rates. For a certain operating condition, the method has better sensitivity to different flow rates. This is suitable for multi-parameter multi-objective optimization of pump impeller. For the test mixed flow pump, the method is more accurate when the area ratios are 13.718% and 13.826%. The cavitation vortex flow is obtained through high-speed camera, and the correlation between cavitation flow structure and cavitation performance is established to provide more scientific support for cavitation performance prediction. The method is not only suitable for cavitation performance prediction of the mixed flow pump, but also can be expanded to cavitation performance prediction of blade type hydraulic machinery, which will solve the problem of rapid prediction of hydraulic machinery cavitation performance.

Numerical and experimental investigation on the diffuser optimization of a reactor coolant pump with orthogonal test approach

Long Yun,Zhu Rongsheng,Wang Dezhong,Yin Junlian,Li Tianbin 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.11

The diffuser of a reactor coolant pump was optimized using an orthogonal approach with numerical simulation to improve the pump hydraulic performance. Steady simulation was conducted by solving Reynolds-averaged Naiver-Stokes equations with the SST k-ω turbulence model using CFX code. The influence of the diffuser geometric parameters, namely, S, φ, α 4 , b 4 , δ 2 , R t and R 4 , on the pump performance were determined. L 18 (3 7 ) orthogonal table was chosen for the optimization process. Best indicators were determined, and range analysis of energy losses, head, and efficiency at the rated condition was performed. Optimal parameters of the diffuser were S = 490 mm, φ = 36°, α 4 = 30°, b 4 = 200 mm, δ 2 = 20 mm, R t = 5 mm and R 4 = 565 mm. The final design was experimentally tested. Simulation results showed more remarkable performance than the experimental result. However, the numerical predictions and experimental results were consistent, validating the design procedure. Loading of the impeller and diffuser blades was analyzed to investigate the direct impact on the hydrodynamic flow field. The head was 14.74 m, efficiency was 79.6 %, and efficiency of the prototype pump was 83.3 % when the model pump functioned at the rated conditions. Optimization results showed that efficiency and head were improved at the design condition.