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배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성
홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-
Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.
Yun-feng Zou,Zheng-yi Fu,Xu-hui He,Hai-quan Jing,Ling-yao Li,Hua-wei Niu,Zheng-qing Chen 한국풍공학회 2019 Wind and Structures, An International Journal (WAS Vol.29 No.4
Wind loading is one of important loadings that should be considered in the design of large hyperbolic natural-draught cooling towers. Both external and internal surfaces of cooling tower are under the action of wind loading for cooling circulating water. In the previous studies, the wind loads on the external surface attracted concernedly attention, while the study on the internal surface was relatively ware. In the present study, the wind pressure on the internal surface of a 220 m high cooling tower is measured through wind tunnel testing, and the effect of ventilation rate of the packing layer on internal pressure is a major concern. The characteristics of internal wind pressure distribution and its effect on wind-induced responses calculated by finite element method are investigated. The results indicate that the wind loading on internal surface of the cooling tower behaves remarkable three-dimensional effect, and the pressure coefficient varies along both of height and circumferential directions. The non-uniformity is particularly strong during the construction stage. Analysis results of the effect of internal pressure on wind-induced responses show that the size and distribution characteristics of internal pressure will have some influence on wind-induced response, however, the outer pressure plays a dominant role in the wind-induced response of cooling tower, and the contribution of internal pressure to the response is small.
( Zheng Jie Liu ),( Jian Wei Yang ),( Chang Zhen Li ),( Jia Xing Li ),( Ya Juan Jiang ),( Yun Hui Dong ),( Yue Yun Li ) 한국화학공학회 2014 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.52 No.6
Polyaniline modified graphene oxide (PANI/GO) composites were synthesized by dilute polymerization technique and were characterized by Fourier transformed infrared spectroscopy (FTIR), Raman spectroscopy, and scanning electron microscopy (SEM). The characterization results indicated that polyaniline molecules were successfully grafted on GO surfaces. The application of PANI/GO composites to the adsorption of heavy metals from aqueous solutions was investigated under ambient conditions. The maximum adsorption capacities of Co(II), Ni(II), Pb(II) and U(VI) ions on PANI/GO composites calculated from Langmuir models are 22.28, 25.67, 65.40 and 1552.31 mg/g, respectively. The excellent adsorption capacity suggests that PANI/GO composites can be applied as a promising adsorbent in heavy metal pollution cleanup in environmental pollution management.
Mouse Orthotopic Lung Cancer Model Induced by PC14PE6
Zheng Yun Cui,안진석,Jee Yun Lee,Won Seog Kim,Ho Yeong Lim,Hyun Jung Jeon,Soo Won Suh,김진훈,공원호,Ji Min Kang,Do Hyun Nam,박근칠 대한암학회 2006 Cancer Research and Treatment Vol.38 No.4
Purpose: This study was undertaken to investigate in detail the xenograft mouse orthotopic lung cancer model induced by PC14PE6 adenocarcinoma cells.Materials and Methods: Three cell doses (0.5×106; 1×106; 2×106) of PC14PE6 cells were injected into the lungs of male BALB/c nude mice by the intrathoracic injection method. The lung and other organs, including brain, liver, spleen, kidney, muscle, adrenal gland, and lymph node on knee, were removed and stained with H/E to detect the presence of tumor cells. Results: The reliable tumorigenicity time in the PC14PE6 adenocarcinoma cell-inoculated BALB/c nude mouse was 10 days after intrathoracic injection. The average life span of the three groups after inoculation was 14 days in the 2×106 cells inoculum group; 25 days in the 1×106 cells inoculum group; and 32 days in the 0.5×106 cells inoculum group. The PC14PE6 adenocarcinoma cells induced orthotopic lung cancer limited within the thorax. Conclusions: This orthotopic lung cancer model is an efficient cancer model with easy inoculation methods, rapid and high tumorigenicity, and simple monitoring methods for metastasis. (Cancer Res Treat. 2006;38:234-239) Purpose: This study was undertaken to investigate in detail the xenograft mouse orthotopic lung cancer model induced by PC14PE6 adenocarcinoma cells.Materials and Methods: Three cell doses (0.5×106; 1×106; 2×106) of PC14PE6 cells were injected into the lungs of male BALB/c nude mice by the intrathoracic injection method. The lung and other organs, including brain, liver, spleen, kidney, muscle, adrenal gland, and lymph node on knee, were removed and stained with H/E to detect the presence of tumor cells.Results: The reliable tumorigenicity time in the PC14PE6 adenocarcinoma cell-inoculated BALB/c nude mouse was 10 days after intrathoracic injection. The average life span of the three groups after inoculation was 14 days in the 2×106 cells inoculum group; 25 days in the 1×106 cells inoculum group; and 32 days in the 0.5×106 cells inoculum group. The PC14PE6 adenocarcinoma cells induced orthotopic lung cancer limited within the thorax.Conclusions: This orthotopic lung cancer model is an efficient cancer model with easy inoculation methods, rapid and high tumorigenicity, and simple monitoring methods for metastasis. (Cancer Res Treat. 2006;38:234-239)
Control Method for Grid-Connected/Islanding Switching of Hybrid AC/DC Microgrid
Qu Zheng-Wei,Chong Zhen-Xiao,Wang Yun-Jing,Shi Zhe,Yao Yun-Xiao 대한전기학회 2023 Vol.18 No.1
For hybrid AC/DC microgrid (HMG) under master–slave control strategy, DGs usually adopt constant power control (P control) in gird-connected mode and at least one DG adopts constant voltage control (V control) in islanding mode. However, when unplanned islanding happens, the voltage and current of the HMG will experience remarkable fl uctuations, which aff ects the system’s stability. This paper presents a control method to achieve smooth switching from grid-connected to islanding mode by introducing state tracking control between P control and V control. The state tracking control can make the current reference signal of the V control track that of the P control in grid-connected mode. When unplanned islanding happens, the mutation of the current reference signal can be tuned into a smooth transition by the voltage loop’s PI controller, and the transient oscillations can be eliminated to realize smooth switching. The eff ectiveness of the proposed control method is verifi ed by simulation results using MATLAB/Simulink.
Li-yun Zheng,Li-xin Zhao,Jing-jun Zhang 한국물리학회 2007 Current Applied Physics Vol.7 No.s1
A new type of material for orthopaedic devices, a three-dimensional braided carbon ber-reinforced nylon 6 (C3D/MCN) composite,was prepared by anionic polymerization. It was characterized by using a block-on-ring wear tester equipped with real-time friction mea-surement capabilities. Scanning electron microscope and an optical microscope were used to study the wear surfaces of the compositesand metallic ring counterpart. The eects of carbon-ber volume fraction, braiding angle, and friction condition on the tribological prop-erties and wear mechanism of C3D/MCN composites were observed. Experiments showed an optimum wear resistance when the carbon-conditions. The wear mechanism under dry sliding conditions is adhesive wear and abrasive wear. The C3D/MCN composites exhibitedmainly abrasive wear under Hank’s-solution-lubricated conditions.
Wenping Zheng,Yaru Liu,Weiwei Zheng,Yunli Xiao,Hongyu Zhang 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.3
RNA interference (RNAi) has been shown to control insect pests using plant dsRNA expression. A key question in potentially applying RNAi is the possible effects on insects after being fed with dsRNA produced by a transgenic plant. Sex peptide receptor (spr) is the key gene that regulates the suppression of female receptivity and induction of oviposition. In this study, the expression level of the spr genewas significantly down-regulated to 52% by feeding Bactrocera dorsalis adults with ds-spr. The RNAi effects of continuous feeding ds-spr to adults led to a highly mortality rate, decreased their egg production capacity and profoundly impacted the eclosion rate of their offspring. Our results demonstrate that RNAi through uninterrupted dsRNA feeding can be used as a strategy to control insect pests. Moreover, the research presented here provides a potential RNAi target gene for controlling B. dorsalis and a theoretical basis for universally applying RNAi in insect pest management.