Nutlin-3 induces HO-1 expression by activating JNK in a transcription-independent manner of p53

CHOE, YUN-JEONG,LEE, SUN-YOUNG,KO, KYUNG WON,SHIN, SEOK JOON,KIM, HO-SHIK Spandidos Publications 2014 International journal of oncology Vol.44 No.3

A recent study reported that p53 can induce HO-1 by directly binding to the putative p53 responsive element in the HO-1 promoter. In this study, we report that nutlin-3, a small molecule antagonist of HDM2, induces the transcription of HO-1 in a transcription-independent manner of p53. Nutlin-3 induced HO-1 expression at the level of transcription in human cancer cells such as U2OS and RKO cells. This induction of HO-1 did not occur in SAOS cells in which p53 was mutated and was prevented by knocking down the p53 protein using p53 siRNA transfection, but not by PFT-alpha, an inhibitor of the transcriptional activity of p53. Accompanying HO-1 expression, nutlin-3 stimulated the accumulation of ROS and the phosphorylation of MAPKs such as JNK, p38 MAPK and ERK1/2. Nutlin-3-induced HO-1 expression was suppressed by TEMPO, a ROS scavenger, and chemical inhibitors of JNK and p38 MAPK but not ERK1/2. In addition, nutlin-3-induced phosphorylation of JNK but not p38 MAPK was inhibited by TEMPO. Notably, the levels of nutlin-3-induced ROS were correlated with the mitochondrial translocation of p53 and this induction was prevented by PFT-beta, an inhibitor of the mitochondrial translocation of p53. Consistent with the effect of the ROS scavenger and MAPK inhibitors, PFT-beta reduced HO-1 expression and the phosphorylation of JNK induced by nutlin-3. In the experiments of analyzing cell death, the knockdown of HO-1 augmented nutlin-3-induced apoptosis. Collectively, these results suggest that nutlin-3 induces HO-1 expression via the activation of both JNK which is dependent on ROS generated by p53 translocated to the mitochondria and p38 MAPK which appears to be stimulated by a ROS-independent mechanism, and this HO-1 induction may inhibit nutlin-3-induced apoptosis, constituting a negative feedback loop of p53-induced apoptosis.

임란 被虜人 홍호연은 누구이며 어떻게 피랍되었는가

신윤호(Shin Yun-ho) 순천향대학교 이순신연구소 2010 이순신연구논총 Vol.- No.14

홍호연(洪浩然, 1582~1657)은 雲海라고도 한다. 1593년, 제2차 진주성전투 이후 나베시마 나오시게 군대에 붙잡혀 일본으로 끌려가 佐賀藩主 나베시마의 家臣이 되어 서예가로 활약했던 인물이다. 그는 조선으로 돌아오려고 하였으나 그 소원을 이루지 못하고 생을 마쳤다. 그가 12세의 어린 나이에 일본으로 건너갔기 때문인지 조선에서의 행적이 남아있지 않다. 그의 출신에 대해서는 거의 알려진 바가 없으며 다만 고향이 산음(현재의 경남 산청지역)이라는 것뿐이었다. 따라서 홍호연의 출생지 및 가족관계를 조사하였으며, 이 과정에서 산청군 오부면 중촌리에 南陽洪氏 마을이 있다는 사실을 발견하였다. 임진왜란 시기에 ‘洪雲海’ 라는 이름이 실려 있는 『南陽洪氏族譜』와 그 일가 인물들의 문집 등을 확보하여 일본 측 사료인『洪浩然傳』과 내용을 대조·분석하였다. 그 결과 홍호연의 아버지는 안제이며, 형제는 맏형 성해, 둘째 형 천해, 동생 진해라는 가족관계를 밝혔다. 400여 년이 지난 이 시점에도 홍호연의 출신을 밝힐 수 있었던 것은 그가 조선 에서 쓰던 성명을 일본에서도 그대로 사용했기 때문이며 그 후손들도 현재까지 홍씨 성을 그대로 유지하고 있었기 때문이다. He is Hong Ho-yeon, penname Un-hae. After(or When) second Jin-ju fortress war broke out at 1593, he was caught by Japanese troops. He became a servant of Saga s Nabesima and then gained a reputation as a calligrapher. He wanted to come back Joseon but ended his life in Japan. At the age of 12, he crossed into Japan. And so, We couldn t find his birth but just knew that his hometown is Saneum (Present Sancheong region, Gyeongnam ). For this reason, I searched for Hong Ho-yeon s birthplace and family relations which almost had disappeared. From these, we found that he had lived in Saneum (Sancheong, Gyeongnam). Comparing「Namyang Mr.Hong genealogy」that includes Hong Un-hae (洪雲海) with「A Hong Ho-yeon s biograpy」written in Japanese, I concluded that his father is An-je, first brother is Sung-hae, second brother is Cheon-hae and younger brother is Jin-hae. He used Joseon name of his own in Japan. so we have easily found Hong Ho-yeon s birth over the past 400 years.

18S-srRNA 분석에 의한 Acantbamoeba 한국 분리주들의 분류 검토

신호준,지영진,조명수,김형일,박연희,임경일 아주대학교 의과학연구소 1998 아주의학 Vol.3 No.2

Acanthamoeba spp. spread in soil, ponds, air and swimming pool, cause a granulomatous amoebic meningitis and amoebic keratitis in human and experimental animals. Their classification had depended upon morphological characteristics and physiological conditions such as thermophilic status and generation times. However, because amoeba species showed the morphological and physiological diversity in isolates, many attempts have been made on identification of Acanthamoeba spp. Restriction fragments length polymorphism (RFLP) analysis of IBS-small subunit ribosomal RNA (srRNA) was an useful tool for classification among morphologically and genetically closely-related species. In this study, 18S-srDNAs of amoebae were amplified by PCR with primer encoding 18S-srRNA gene and digested with restriction endonudeases, and RFLP analysis was applied on classification of Acanthamoeba Korean isolates (YM-4, YM-5 and YM-7), comparing with reference amoebae, A. culbertsoni, A. polyphaga and A. royreba. Trophozoites of six Acanthamoeba spp. had typical acanthopoda, but did not show any morphological differences. Cyst of Acanthamoeba sp. YM-7 was similar to that of A. polyphaga which was designated as group Ⅱ Acanthamoeba. Morphologically other amoebae belonged to group Ⅲ Acanthamoeba. Digestion of 18S-srDNA with six enzymes, Dde Ⅰ, Hae Ⅲ, Hind Ⅲ, EcoR Ⅰ, Rsa Ⅰ and Sph Ⅰ, resulted in various DNA fragments. Using the method of Nei and U (1979) for RFLP analysis, genetic divergence was not observed between A culbertsoni and Acanthamoeba sp. YM-4. Between A. culbertsoni and Acanthamoeba sp. YM-5, genetic distance was 0.070, 0.364 between Acanthamoeba sp. YM-4 and A. polyphaga, and 0.277 between Acanthamoeba sp. YM-7 and A. polyphaga. In comparison with A. culbertsoni and A. polyphaga, Acanthamoeba sp. YM-7 showed genetic distance of 0.330 and 0.154, respectively. Thus, Acanthamoeba sp. YM-7 is a similar species to A. polyphaga. Acanthamoeba sp. YM-5 is a different species from A. polyphaga, but closely-related to A. culbertsoni. Acanthamoeba sp. YM-4 can be classified as a subspecies or a strain of A. culbertsoni.

만성 알코올중동증의 치료에서 프리달의 임상효과

신윤오,신석철,왕성근,유진호,윤미숙 최신의학사 1992 最新醫學 Vol.35 No.4

두부손상 환자의 정신과적 합병증에 관한 장애 감정례의 일반 사항에 관한 분석

신석철,왕성근,신윤오,김덕호,김상국,강동숙 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

Authorst ried retrospective analysis to find out general characteristics of the patients undergoing disability evaluation at the department of psychiatry Chungnam National University Hospital from 1986 to 1990. The subjects, who were suffered from psychiatric complications due to head trauma by traffic accident, were 66 patients and they were classified depending on the demographic status, situations of traffic accident, evalaution periods, whether or not perform operation, and rates of evaluated disability and diagnosis. The results were summarized as follows. 1. General background of the subjects were as follows. 1) The frequency order of request were court(59 %) and insurance company(19.7%). 2) The place of accident was more common in large city, common accident vehicle were bus, truck, and cabs. 3) The season of accident were more common in Spring and Autumn. 2. Male(71.2%) was more common than in female and more common in the age group of 5-19 years old(37.9%). The most frequent job of the patients was labour, and the education level was more common in the graduates of elementary school(47.9% ). 3. The interval between accident and evaluation was most frequent in 12-23 months(47.5%), and duration of evaluation was mostly 14 to 21 days. Performed brain operation was 31.8% and non-operated patients was 50.0%. The rates of disability according to the McBride's disability evaluation were mostly belonged to the group of beow 40%(74.1%). 4. The final diagnosis after evaluation were organic personality disorder(45.5%) and dementia(31.8%).

유한요소해석에 의한 절대코일 와전류 신호의 임피던스 평면도 작성

신영길,이윤태,이정호,송명호 한국비파괴검사학회 2004 한국비파괴검사학회지 Vol.24 No.4

와전류 탐상에서 차동형 탐촉자는 신호에 영향을 미치는 변수들을 줄일 수 있어 흔히 사용된다. 그러나 차동신호는 인접한 두 코일의 임피던스 차이를 신호로 사용하기 때문에 신호예측이나 해석이 쉽지 않다는 단점이 있다. 반면에 절대코일에 의한 신호는 상대적으로 형태가 단순하므로 신호예측이나 해석이 더 수월하다. 따라서 서로의 장, 단점을 상호보완적으로 사용하면 검사 신뢰도를 향상시키는데 큰 도움이 될 것이다. 본 논문에서는 인코넬 평판과 튜브에서 절대코일 신호를 예측하기 위하여 유한요소해석을 수행하였고, 리프트 오프, 충전율, 전도도, 탐상 주파수, 피검사체의 두께, 내, 외부 결함 등이 신호에 미치는 영향을 계산하여 임피던스 평면도로 작성하고, 신호특징을 분석하였다. 그 결과, 절대신호에 대한 많은 실용적인 지식을 축적할 수 있었고, 절대신호와 차동신호 특성의 유사성을 이해하게 되었으며, 결함깊이와 주파수 변화에 따른 신호의 기울기를 대응곡선 그래프로 작성할 수 있었다. In eddy current testing(ECT), differential probes have been frequently used since they can reduce the number of parameters that influcence ECT singles. However, differential signal is actually the difference of the two coil's impedance so that signal prediction and interpretation are not easy. On the other hand, absolute coil signal is rather straightforward to predict and analyze. Therefore, combined use of the two types of signals would increase the test reliability. In this paper, absolute coil signals from Inconel plate and tubes are predicted by the finite element analysis and effects of lift-off, fill-factor, conductivity, operating frequency, test specimen thickness, inner diameter defects, and outer diameter defects are investigated. As a result, various impedance plane diagrams are drawn and analyzed. Significant practical knowledge about absolute signals is accumulated and similar characteristics of the two types of signal could be understood. Finally, slope angle versus defect depth calibration curves are prepared for three different frequencies.

濟州道公務員의 特性과 庶政刷新에 관한 硏究

申宗淳,鄭允武,朴瑾浩,金武昌 동국대학교 행정대학원 1977 행정논집 Vol.7 No.-

직경 1˝ 자성관에서의 원격장와전류 탐상 실험 빛 결함신호 특성

신영길,이창준,이정호,이윤태,장권규,송성철,송성진,정태언 국립7개대학공동논문집간행위원회 2002 공업기술연구 Vol.2 No.-

In this study, a remote field eddy current testing system is built to inspect 1˝ diameter ferromagnetic tubes and resulting experimental signals are investigated. To decide the operating frequency, the emf of sensor coil is monitored while the sensor coil is moved away from the exciter. At proper frequency, sudden change would occur in the signal pattern. This also indicates the location of remote field region, so that the distance between coils can be decided. The defect signal contains two defect indications, each appears as the sensor and exciter coil passes a defect. Experimental results show that defect depth can be estimated by comparing the two indications. Also, linear relationship between defect depth and phase signal strength is observed.

우울증 선별 검사 도구로서 Beck Depression Inventory(BDI)의 타당성 : Detection of depression in primary care

신호철,김철환,박용우,조비룡,송상욱,윤영호,오상우 大韓家庭醫學會 2000 Korean Journal of Family Medicine Vol.21 No.11

가스관의 원격장와전류 탐상실험에서 관찰된 결함신호의 특성

신영길,이정호,이윤태,송성진,정태언 국립7개대학공동논문집간행위원회 2003 공업기술연구 Vol.3 No.-

When the identical coils are used for the exciter and sensor in the remote field eddy current (RFEC) probe, two defect indications appear in a single defect signal. The two indications are expected to be the same according to numerical simulation study. However, in our previous experimental work on 1" diameter ferromagnetic tube, we had found that the two indications in a phase signal are not identical and it may be possible to estimate the defect depth by comparing the two indications in a single phase signal. In this paper, those findings are re-examined with gas pipes whose diameter is 48.8 mm. At first, the operating frequency and the coil spacing in a probe are decided. Secondly, defect specimens are designed and built after examining edge effects and the signal sensitivity to the distance between sensor and defect. The RFEC testing experiments using these specimens show the same tendency as appeared in 1" diameter tube, i.e., the defect indication that appears in a phase signal as the exciter coil passes a defect is smaller for shallow defects, but it becomes bigger than the other for deep defects. This finding seems to be very important since it enables us to estimate the defect depth without preparing calibration curves for various depths of defects.