Two new triterpenoid saponins derived from the leaves of Panax ginseng and their antiinflammatory activity

Fuli Li,Yufeng Cao,Tingwu Liu,Guilong Yan,Liang Chen,Lilian Ji,Lun Wang,Bin Chen,Aftab Yaseen,Ashfaq A. Khan,Guo-Lin Zhang,Yunyao Jiang,Jianxun Liu,Gongcheng Wang,Ming-Kui Wang,Weicheng Hu 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.4

Background: The leaves and roots of Panax ginseng are rich in ginsenosides. However, the chemical compositionsof the leaves and roots of P. ginseng differ, resulting in different medicinal functions. In recent years,the aerial parts of members of the Panax genus have received great attention fromnatural product chemistsas producers of bioactive ginsenosides. The aim of this study was the isolation and structural elucidation ofnovel, minor ginsenosides in the leaves of P. ginseng and evaluation of their antiinflammatory activity in vitro. Methods: Various chromatographic techniques were applied to obtain pure individual compounds, andtheir structures were determined by nuclear magnetic resonance and high-resolution mass spectrometry,as well as chemical methods. The antiinflammatory effect of the new compounds was evaluated onlipopolysaccharide-stimulated RAW 264.7 cells. Results and conclusions: Two novel, minor triterpenoid saponins, ginsenoside LS1 (1) and 5,6-didehydroginsenoside Rg3 (2), were isolated from the leaves of P. ginseng. The isolated compounds 1and 2 were assayed for their inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7cells, and Compound 2 showed a significant inhibitory effect with IC50 of 37.38 mM compared with that ofNG-monomethyl-L-arginine (IC50 ¼ 90.76 mM). Moreover, Compound 2 significantly decreased secretionof cytokines such as prostaglandin E2 and tumor necrosis factor-a. In addition, Compound 2 significantlysuppressed protein expression of inducible nitric oxide synthase and cyclooxygenase-2. These resultssuggested that Compound 2 could be used as a valuable candidate for medicinal use or functional food,and the mechanism is warranted for further exploration.

Two new triterpenoid saponins derived from the leaves of Panax ginseng and their antiinflammatory activity

Li, Fu,Cao, Yufeng,Luo, Yanyan,Liu, Tingwu,Yan, Guilong,Chen, Liang,Ji, Lilian,Wang, Lun,Chen, Bin,Yaseen, Aftab,Khan, Ashfaq A.,Zhang, Guolin,Jiang, Yunyao,Liu, Jianxun,Wang, Gongcheng,Wang, Ming-Kui The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.4

Background: The leaves and roots of Panax ginseng are rich in ginsenosides. However, the chemical compositions of the leaves and roots of P. ginseng differ, resulting in different medicinal functions. In recent years, the aerial parts of members of the Panax genus have received great attention from natural product chemists as producers of bioactive ginsenosides. The aim of this study was the isolation and structural elucidation of novel, minor ginsenosides in the leaves of P. ginseng and evaluation of their antiinflammatory activity in vitro. Methods: Various chromatographic techniques were applied to obtain pure individual compounds, and their structures were determined by nuclear magnetic resonance and high-resolution mass spectrometry, as well as chemical methods. The antiinflammatory effect of the new compounds was evaluated on lipopolysaccharide-stimulated RAW 264.7 cells. Results and conclusions: Two novel, minor triterpenoid saponins, ginsenoside $LS_1$ (1) and 5,6-didehydroginsenoside $Rg_3$ (2), were isolated from the leaves of P. ginseng. The isolated compounds 1 and 2 were assayed for their inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7 cells, and Compound 2 showed a significant inhibitory effect with $IC_{50}$ of $37.38{\mu}M$ compared with that of NG-monomethyl-L-arginine ($IC_{50}=90.76{\mu}M$). Moreover, Compound 2 significantly decreased secretion of cytokines such as prostaglandin $E_2$ and tumor necrosis factor-${\alpha}$. In addition, Compound 2 significantly suppressed protein expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggested that Compound 2 could be used as a valuable candidate for medicinal use or functional food, and the mechanism is warranted for further exploration.

Effect of Temperature and Strain Rate on Deformation Mode and Crack Behavior of 7B52 Laminated Aluminum Alloy Under Impact Loading

Wenhui Liu,Ping Cao,Chenbing Zhao,Yufeng Song,Changping Tang 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.11

The dynamic mechanical behaviors of 7B52 laminated aluminum alloy under different impact deformation conditions wereinvestigated using split Hopkinson pressure bar, optical microscopy, electron backscatter diffraction and transmission electronmicroscopy. The results show that the 7B52 laminated aluminum alloy displays positive strain rate sensitivity and negativetemperature sensitivity. Cracks are formed firstly at the interface between the 7A62 hard layer and the 7A01 middle layerunder a low strain rate of 2200 s−1. When the strain rate increases to 3500 s−1, the deformation shear band is firstly formed inthe 7A52 soft layer. The deformation shear band transformed to the transition shear band with the increase of strain rate, andthen crack initiated. Adiabatic shear bands and cracks are formed in both of the hard and soft layers when samples impactedat a high strain rate (5500 s−1). Most of the cracks and adiabatic shear bands are unable to penetrate the middle layer dueto the good toughness of the 7A01 alloy. The deformation of each layer of 7B52 laminated aluminum alloy becomes moreuniform when the temperature increases, which was attributed to the reduction of difference in strength among the threelayers and the coordinating effect of grain boundary on deformation.

Frequency and magnetic properties in the range of 10 kHz to 100 MHz for nanocrystal Fe -Co alloy

Yongsheng Liu,Jincang Zhang,Liming Yu,Guangqiang Jia,Yufeng Zhang,Xinyan Wang,Shixun Cao 한국물리학회 2004 Current Applied Physics Vol.4 No.5

Fe-based soft magnetic alloy with Co-doping was fabricated by high-energy milling. The X-ray diraction (XRD) results exhibita single phased structure,which could be regarded as BCC-like FeCo solid solution,was formed. Moreover,it has also been demonstrated that a nanocrystalline FeCo alloy was obtained and the evaluated grain sizes were about 712 nm. The frequency dependence of the initial permeability and magnetic losses,as well as DC-magnetization measurements,was systematically studiedthen. The results show that thefn (fn is the frequency when the curve of the No.n sample has a minimum value) shifts to the higherfrequency with the increasing Co content in the range of 10 kHz to 100 MHz,while the magnetic losses of the samples decrease withthe increasing Co content at the high frequency (800 kHz to 20 MHz). The Co-doping increases the cut-o frequencies of thesamples and makes the initial permeability to keep a constant value in a wider frequency range. The corresponding peak value of theinitial permeability as function of Co-doping concentration turns to the region of low Co concentration. For the long milling time,the loss apparently decreases beyond 1 MHz.

Study on Preparation and Properties of PAI Materials Containing Trifluoromethyl in Side Chain

Haiyang Yang,Duxin Li,Jun Yang,Jin Wang,Shunchang Gan,Kaikai Cao,Yufeng Liu 한국고분자학회 2021 Macromolecular Research Vol.29 No.10

Polyamide-imide (PAI) materials with good heat resistance, excellent friction, wear properties, and low water absorption were prepared by acyl chloride process with diamine monomer containing trifluoromethyl in the side chain. The results showed that PAI prepared from 2,2'-bis(trifluoromethyl)-[1,1'-biphenyl]- 4,4'-diamine without flexible hinge group had the best heat resistance, with T g as high as 302.6℃ and T d5% as 490.1℃. With the increase of the proportion of flexible hinge groups in diamine monomer, the thermal properties of PAI materials would decrease. The wear mechanism of PAI prepared from 2,2'-bis(trifluoromethyl)- [1,1'-biphenyl]-4,4'-diamine was mainly adhesive wear. With the increase of the proportion of flexible hinge groups in diamine monomer, the wear mechanism would change from abrasive wear, slight adhesive wear to severe adhesive wear. The friction coefficient and wear loss of PAI prepared by 1,4-bis(4-amino-2-trifluoromethylphenoxy) benzene were the smallest, which were 0.4706 and 3.5, respectively. PAI synthesized by diamine monomer containing trifluoromethyl had low water absorption and high water contact angle. With the increasing flexibility of monomer containing trifluoromethyl diamine, the elongation at break and tensile strength of the material increased gradually.

The impacts of LPCVD wrap-around on the performance of n-type tunnel oxide passivated contact c-Si solar cell

Zhou Ying,Tao Ke,Liu Aimin,Rui Jia,Bao Jianhui,Sun Yufeng,Yang Sanchuan,Wang Qinqin,Zhang Qiang,Yang Songbo,Cao Yujia,Qu Hui 한국물리학회 2020 Current Applied Physics Vol.20 No.7

In this paper, Tunnel Oxide Passivated Contact (TOPCon) silicon solar cells with the industrial area (244.32 cm2) are fabricated on N-type silicon substrates. Both the ultra-thin tunnel oxide layer and phosphorus doped polycrystalline silicon (polysilicon) thin film are prepared by the LPCVD system. The wrap-around of polysilicon is observed on the surface of borosilicate glass (BSG). The polysilicon wrap-around can form a leakage current path, thus degrades the shunt resistance of solar cells, and leads to the degradation of solar cell efficiency. Different methods are adopted to treat the polysilicon wrap-around and improve shunt resistance of solar cells. The experimental results indicate that a chemical etching method can effectively solve the problem of polysilicon wrap-around and improve the performance of solar cells. Finally, a conversion efficiency of 22.81% has been achieved by our bifacial TOPCon solar cells, with Voc of 702.6 mV, Jsc of 39.78 mA/cm2 and FF of 81.62%.

Multi-channel 5Gb/s/ch SERDES with Emphasis on Integrated Novel Clocking Strategies

Zhang, Changchun,Li, Ming,Wang, Zhigong,Yin, Kuiying,Deng, Qing,Guo, Yufeng,Cao, Zhengjun,Liu, Leilei The Institute of Electronics and Information Engin 2013 Journal of semiconductor technology and science Vol.13 No.4

Two novel clocking strategies for a high-speed multi-channel serializer-deserializer (SERDES) are proposed in this paper. Both of the clocking strategies are based on groups, which facilitate flexibility and expansibility of the SERDES. One clocking strategy is applicable to moderate parallel I/O cases, such as high density, short distance, consistent media, high temperature variation, which is used for the serializer array. Each group within the strategy consists of a full-rate phase-locked loop (PLL), a full-rate delay-locked loop (DLL), and two fixed phase alignment (FPA) techniques. The other is applicable to more awful I/O cases such as higher speed, longer distance, inconsistent media, serious crosstalk, which is used for the deserializer array. Each group within the strategy is composed of a PLL and two DLLs. Moreover, a half-rate version is chosen to realize the desired function of 1:2 deserializer. Based on the proposed clocking strategies, two representative ICs for each group of SERDES are designed and fabricated in a standard $0.18{\mu}m$ CMOS technology. Measurement results indicate that the two SERDES ICs can work properly accompanied with their corresponding clocking strategies.

Hepatitis B virus (HBV) codon adapts well to the gene expression profile of liver cancer: an evolutionary explanation for HBV’s oncogenic role

Chunpeng Yu,Jian Li,Qun Li,Shuai Chang,Yufeng Cao,Hui Jiang,Lingling Xie,Gang Fan,Song Wang 한국미생물학회 2022 The journal of microbiology Vol.60 No.11

Due to the evolutionary arms race between hosts and viruses, viruses must adapt to host translation systems to rapidly synthesize viral proteins. Highly expressed genes in hosts have a codon bias related to tRNA abundance, the primary RNA translation rate determinant. We calculated the relative synonymous codon usage (RSCU) of three hepatitis viruses (HAV, HBV, and HCV), SARS-CoV-2, 30 human tissues, and hepatocellular carcinoma (HCC). After comparing RSCU between viruses and human tissues, we calculated the codon adaptation index (CAI) of viral and human genes. HBV and HCV showed the highest correlations with HCC and the normal liver, while SARS-CoV-2 had the strongest association with lungs. In addition, based on HCC RSCU, the CAI of HBV and HCV genes was the highest. HBV and HCV preferentially adapt to the tRNA pool in HCC, facilitating viral RNA translation. After an initial trigger, rapid HBV/HCV translation and replication may change normal liver cells into HCC cells. Our findings reveal a novel perspective on virus-mediated oncogenesis.

Multi-channel 5Gb/s/ch SERDES with Emphasis on Integrated Novel Clocking Strategies

Changchun Zhang,Ming Li,Zhigong Wang,Kuiying Yin,Qing Deng,Yufeng Guo,Zhengjun Cao,Leilei Liu 대한전자공학회 2013 Journal of semiconductor technology and science Vol.13 No.4

Two novel clocking strategies for a high-speed multi-channel serializer-deserializer (SERDES) are proposed in this paper. Both of the clocking strategies are based on groups, which facilitate flexibility and expansibility of the SERDES. One clocking strategy is applicable to moderate parallel I/O cases, such as high density, short distance, consistent media, high temperature variation, which is used for the serializer array. Each group within the strategy consists of full-rate phase-locked loop (PLL), a full-rate delay-locked loop (DLL), and t재 fixed phase alignment (FPA) techniques. The other is applicable to more awful I/O cases such as higher speed, longer distance, inconsistent media, serious crosstalk, which is used for the deserializer array. Each group within the strategy is composed of a PLL and two DLLs. Moreover, a half-rate version is chosen to realize the desired function of 1:2 deserializer. Based on the proposed clocking strategies, two representative ICs for each group of SERDES are designed and fabricated in a standard 0.18㎛ CMOS technology. Measurement results indicate that the two SERDES ICs can work properly accompanied with their corresponding clocking strategies.