Effect of P3HT passivation layer on triple cation organic-inorganic hybrid perovskite memristor

Wang Yucheng,Xiong Yuxuan,Wang Hongsu,Wu Xiang,Sha Jian,Shang Yueyang,Zhang Yongqi,Li Wei,Wang Shaoxi 한국물리학회 2023 Current Applied Physics Vol.47 No.-

Triple cation organic-inorganic hybrid perovskite (TCP) memristor is a novel candidate for artificial visual applications due to the integrating of recognition, storage, and computing. However, concerns remain regarding the moisture-induced degradation and thermal stability. Here, P3HT was deposited on the TCP film to optimize the memristor performance which showed better film surface quality and lower power consumption. When the thickness of the P3HT layer increases, the grain boundary of the TCP film was gradually filled, leading to the inhibition of the ions/vacancies drifting, and thus the device type was changed from digital to analog. Besides, various functions are demonstrated in TCP photoelectric memristor including excitatory postsynaptic current under optic and electric stimulations and spike rate-dependent plasticity, proving the great application prospects in numerical calculation and information perception.

Overexpression of a peroxiredoxin gene from Tamarix hispida, ThPrx1, confers tolerance to oxidative stress in yeast and Arabidopsis

Liuqiang Wang,Zhen Li,Chao Wang,Deyin Wang,Yucheng Wang,Mengzhu Lu 한국식물학회 2017 Journal of Plant Biology Vol.60 No.6

Peroxiredoxins (Prxs) are ubiquitous thiol-specificantioxidant enzymes that are critically involved in cell defenseand protect cells from oxidative damage. In this study, aputative Type II Prx (ThPrx1) was identified and characterizedfrom Tamarix hispida. The expression of ThPrx1 is highlyinduced in response to hydrogen peroxide (H2O2) and methylviologen (MV) stresses. When expressed ectopically, ThPrx1showed enhanced tolerance against oxidative stress in yeastand Arabidopsis. In addition, transgenic Arabidopsis plantsoverexpressing ThPrx1 displayed improved seedling survivalrates and increased root growth and fresh weight gain underH2O2 and MV treatments. Moreover, transgenic Arabidopsisplants showed decreased accumulation of H2O2, superoxide(O2•−) and malondialdehyde (MDA), increased superoxidedismutase (SOD) activity compared to wild-type (WT) plantsunder oxidative stress. Moreover, transgenic plants maintainedhigher photosynthesis efficiency and lower electrolyte leakagerates than that of WT plants under stress conditions. Theseresults clearly indicated that ThPrx1 plays an important rolein cellular redox homeostasis under stress conditions, leadingto the maintenance of membrane integrity and increasedtolerance to oxidative stress.

Characteristics of La_2O_3―Y_2O_3―Mo cermet cathode with RE_2O_3 nano particles

Jinshu Wang,Wei Liu,Zhiyuan Ren,Fan Yang,Yiman Wang,Yucheng Du,Meiling Zhou 한국물리학회 2011 Current Applied Physics Vol.11 No.3

The secondary electron emission property and microstructure of La_2O_3―Y_2O_3―Mo cermet cathode have been studied. It shows that the cathode prepared by Sol―Gel doping method has fine microstructure containing rare earth oxide (RE_2O_3) nanoparticles and uniform distribution of different substances. Auger Electron Spectroscopy (AES) depth profile result shows that an RE_2O_3 layer about 28 nm in thickness could be formed on the cathode surface, which plays an important role in the emission. Pre-activation of the cathode is favorable for the improvement of emission property. La_2O_3―Y_2O_3―Mo cathode preactivated exhibits good secondary emission property. The secondary emission yield could reach 5.24,about 1.8 times higher than that of original cathode. The enhancement on the conductivity of materials resulting from the pre-activation is favorable for the replenishment and transport of electrons, thus the secondary emission property of the cathode could be improved.

Study of co-excited green emission of Tb3+, Ce3+ and Gd3+ in yttrium aluminum garnet

Fei Huang,Limin Dong,Hao Wang,Weimin Wang,Yucheng Wang,Zhengyi Fu 한양대학교 세라믹연구소 2009 Journal of Ceramic Processing Research Vol.10 No.6

YAG : Tb3+, Ce3+, Gd3+ nano-phosphors derived from a sol-gel chemistry have been successfully synthesized and characterized by TG/DTA, XRD, SEM, and spectrometer. The results show that the phosphors have a uniform particle size distribution ranging 30-50 nm. The value of the lattice constant increased with an increase of the Gd3+ content. Tb3+ →Ce3+, Ce3+ →Tb3+ and Gd3+ →Ce3+ energy transfer existed in the co-excited system. The luminescence intensity was controlled by the concentration and ratio of the co-dopants. A small quantity of Gd3+ and Ce3+ evidently increased the green emission (5D4→7F5) of Tb3+. YAG : Tb3+, Ce3+, Gd3+ nano-phosphors derived from a sol-gel chemistry have been successfully synthesized and characterized by TG/DTA, XRD, SEM, and spectrometer. The results show that the phosphors have a uniform particle size distribution ranging 30-50 nm. The value of the lattice constant increased with an increase of the Gd3+ content. Tb3+ →Ce3+, Ce3+ →Tb3+ and Gd3+ →Ce3+ energy transfer existed in the co-excited system. The luminescence intensity was controlled by the concentration and ratio of the co-dopants. A small quantity of Gd3+ and Ce3+ evidently increased the green emission (5D4→7F5) of Tb3+.

Genome-wide identification of WRKY family genes and their response to abiotic stresses in tea plant (Camellia sinensis)

Pengjie Wang,Chuan Yue,Di Chen,Yucheng Zheng,Qian Zhang,Jiangfan Yang,Naixing Ye 한국유전학회 2019 Genes & Genomics Vol.41 No.1

The WRKY transcription factors (TFs) family is one of the largest TF families in plants and plays a central role in diverse regulation and multiple stress responses. However, the systematical analysis of the WRKY gene family in tea plant (Camellia sinensis) based on genomic data has been lacking. The primary objective of this study was to set a systematic analysis of the WRKY gene family based on genomic data in tea plant and analyze their expression profiles under various abiotic stresses. We searched the tea plant genome using the consensus model of the WRKY domain (PF03106) and then used these search results to identify all the WRKY family members by SMART and the CDD program. Analyze their phylogeny, classification, structure, conserved motifs, Cis-elements, interactors and expression profiles. 56 putative WRKY genes were identified from the tea plant genome and divided into three main groups (I–III) and five subgroups (IIa–IIe) according to the WRKY domains and the zinc-finger structure. The gene structure and conserved motifs of the CsWRKY genes were also characterized and were consistent with the classification results. Annotation analysis showed that 34 CsWRKY genes may be involved in stress responses. Promoter analysis implied that CsWRKY genes, except for CsWRKY55, possessed at least one abiotic stress response cis-element. Expression profiles of CsWRKY genes in different tissues were analyzed with RNA-seq data. The results showed that 56 CsWRKY genes had differential expression in their transcript abundance. The expression profiles also showed that many identified CsWRKY genes were possibly involved in the response to cold, drought, salt, or ABA treatment. Tea plant genome contains at least 56 WRKY genes. These results provide useful information for further exploring the function and regulatory mechanism of CsWRKY genes in the growth, development, and adaption to abiotic stresses in tea plant.

Molecular Characteristics and Potent Immunomodulatory Activity of Fasciola hepatica Cystatin

Kai Zhang,Yucheng Liu,Guowu Zhang,Xifeng Wang,Zhiyuan Li,Yunxia Shang,Chengcheng Ning,Chunhui Ji,Xuepeng Cai,Xianzhu Xia,Jun Qiao,Qingling Meng 대한기생충학ㆍ열대의학회 2022 The Korean Journal of Parasitology Vol.60 No.2

Cystatin, a cysteine protease inhibitor found in many parasites, plays important roles in immune evasion. This study analyzed the molecular characteristics of a cystatin from Fasciola hepatica (FhCystatin) and expressed recombinant FhCystatin (rFhcystatin) to investigate the immune modulatory effects on lipopolysaccharide-induced proliferation, migration, cytokine secretion, nitric oxide (NO) production, and apoptosis in mouse macrophages. The FhCystatin gene encoded 116 amino acids and contained a conserved cystatin-like domain. rFhCystatin significantly inhibited the activity of cathepsin B. rFhCystatin bound to the surface of mouse RAW264.7 cells, significantly inhibited cell proliferation and promoted apoptosis. Moreover, rFhCystatin inhibited the expression of cellular nitric oxide, interleukin-6, and tumor necrosis factor-α, and promoted the expression of transforming growth factor-β and interleukin-10. These results showed that FhCystatin played an important role in regulating the activity of mouse macrophages. Our findings provide new insights into mechanisms underlying the immune evasion and contribute to the exploration of potential targets for the development of new drug to control F. hepatica infection.

Aero-elastic wind tunnel test of a high lighting pole

Luo, Yaozhi,Wang, Yucheng,Xie, Jiming,Yang, Chao,Zheng, Yanfeng Techno-Press 2017 Wind and Structures, An International Journal (WAS Vol.25 No.1

This paper presents a 1:25 multi-freedom aero-elastic model for a high lighting pole at the Zhoushan stadium. To validate the similarity characteristics of the model, a free vibration test was performed before the formal test. Beat phenomenon was found and eliminated by synthesis of vibration in the X and Y directions, and the damping ratio of the model was identified by the free decay method. The dynamic characteristics of the model were examined and compared with the real structure; the similarity results were favorable. From the test results, the major along-wind dynamic response was the first vibration component. The along-wind wind vibration coefficient was calculated by the China code and Eurocode. When the peak factor equaled 3.5, the coefficient calculated by the China code was close to the experimental result while Eurocode had a slight overestimation of the coefficient. The wind vibration coefficient during typhoon flow was analyzed, and a magnification factor was suggested in typhoon-prone areas. By analyzing the power spectrum of the dynamic cross-wind base shear force, it was found that a second-order vortex-excited resonance existed. The cross-wind response in the test was smaller than Eurocode estimation. The aerodynamic damping ratio was calculated by random decrement technique and the results showed that aerodynamic damping ratios were mostly positive at the design wind speed, which means that the wind-induced galloping phenomenon is predicted not to occur at design wind speeds.

Aero-elastic wind tunnel test of a high lighting pole

Yaozhi Luo,Yucheng Wang,Jiming Xie,Chao Yang,Yanfeng Zheng 한국풍공학회 2017 Wind and Structures, An International Journal (WAS Vol.25 No.1

This paper presents a 1:25 multi-freedom aero-elastic model for a high lighting pole at the Zhoushan stadium. To validate the similarity characteristics of the model, a free vibration test was performed before the formal test. Beat phenomenon was found and eliminated by synthesis of vibration in the X and Y directions, and the damping ratio of the model was identified by the free decay method. The dynamic characteristics of the model were examined and compared with the real structure; the similarity results were favorable. From the test results, the major along-wind dynamic response was the first vibration component. The along-wind wind vibration coefficient was calculated by the China code and Eurocode. When the peak factor equaled 3.5, the coefficient calculated by the China code was close to the experimental result while Eurocode had a slight overestimation of the coefficient. The wind vibration coefficient during typhoon flow was analyzed, and a magnification factor was suggested in typhoon-prone areas. By analyzing the power spectrum of the dynamic cross-wind base shear force, it was found that a second-order vortex-excited resonance existed. The cross-wind response in the test was smaller than Eurocode estimation. The aerodynamic damping ratio was calculated by random decrement technique and the results showed that aerodynamic damping ratios were mostly positive at the design wind speed, which means that the wind-induced galloping phenomenon is predicted not to occur at design wind speeds.

Modification of activated carbon from agricultural waste lotus leaf and its adsorption mechanism of beryllium

Xu Zhao,Yucheng Su,Hongqiang Wang,Zhiwu Lei,Eming Hu,Fang Hu,Qingliang Wang,Lechang Xu,Shiyao Fan,Xinwei Liu,Xuanzhang Hao 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.1

With the wide application of beryllium globally, industrial wastewater has rapidly increased. Previously, adsorption was effective in treating this issue. However, most adsorbents have a poor removal rate, primarily in the low adsorption capacity. To remove Be from industrial wastewater and overcome the disadvantages of low adsorption capacity and poor removal rate of existing adsorbents, typical agricultural waste lotus leaf was used to prepare Al-activated carbon (Al-AC) by the impregnation-calcination modification of Al(NO3)3. The theoretical maximum adsorption capacity of Al-AC was 32.86 mg/g. Langmuir, Freundlich, and Temkin models were used to thermodynamically analyze Al-AC, and adsorption thermodynamics demonstrated that the adsorption reaction of Al-AC was endothermic. Through characterization analysis, the specific surface area of the modified AC increased from 4.3573 to 155.87 m2/g. This study provides a new approach to preparing and modifying AC and a new method for removing Be from industrial wastewater.

Biodegradable charged polyester-based vectors (BCPVs) as an efficient non-viral transfection nanoagent for gene knockdown of the BCR-ABL hybrid oncogene in a human chronic myeloid leukemia cell line

Yang, Chengbin,Panwar, Nishtha,Wang, Yucheng,Zhang, Butian,Liu, Maixian,Toh, Huiting,Yoon, Ho Sup,Tjin, Swee Chuan,Chong, Peter Han Joo,Law, Wing-Cheung,Chen, Chih-Kuang,Yong, Ken-Tye The Royal Society of Chemistry 2016 Nanoscale Vol.8 No.17

<P>First-line therapy of chronic myelogenous leukemia (CML) has always involved the use of BCR-ABL tyrosine-kinase inhibitors which is associated with an abnormal chromosome called Philadelphia chromosome. Although the overall survival rate has been improved by the current therapeutic regime, the presence of resistance has resulted in limited efficacy. In this study, an RNA interference (RNAi)-based therapeutic regime is proposed with the aim to knockdown the BCR-ABL hybrid oncogene using small interfering RNA (siRNA). The siRNA transfection rates have usually been limited due to the declining contact probability among polyplexes and the non-adherent nature of leukemic cells. Our work aims at addressing this limitation by using a biodegradable charged polyester-based vector (BCPV) as a nano-carrier for the delivery of BCR-ABL-specific siRNA to the suspension culture of a K562 CML cell line. BCR-ABL siRNAs were encapsulated in the BCPVs by electrostatic force. Cell internalization was facilitated by the BCPV and assessed by confocal microscopy and flow cytometry. The regulation of the BCR-ABL level in K562 cells as a result of RNAi was analyzed by real-time polymerase chain reaction (RT-PCR). We observed that BCPV was able to form stable nanoplexes with siRNA molecules, even in the presence of fetal bovine serum (FBS), and successfully assisted in vitro siRNA transfection in the non-adherent K562 cells. As a consequence of downregulation of BCR-ABL, BCPV-siRNA nanoplexes inhibited cell proliferation and promoted cell apoptosis. All results were compared with a commercial transfection reagent, Lipofectamine2000 (TM), which served as a positive control. More importantly, this class of non-viral vector exhibits biodegradable features and negligible cytotoxicity, thus providing a versatile platform to deliver siRNA to non-adherent leukemia cells with high transfection efficiency by effectively overcoming extra-and intra-cellular barriers. Due to the excellent in vitro transfection results from BCPV-siRNA, a newly developed biodegradable transfection agent, BCPV, is being probed for transfection performance in an animal model.</P>