Serial values for hematologic and biochemical analysis after myocardial infarction in rats

( Mi Jin Lee ),( Hyun Jin Tae ),( Ying Hua Li ),( Do Hyeon Yu ),( In Ae Han ),( Seok Won Lee ),( Dong Choon Ahn ),( In Shik Kim ),( Jin Ho Park ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.2

To diagnose acute myocardial infarction(MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hema-tologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and bioche-mical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery(LMDA) of the left coronary artery was ligated during the progression(day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group(n=24, thoracotomy without LMDA ligation) and the experimental(MI) group(n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell(RBCs), hemoglobin and hematocrit(HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, choles-terol, total creatine kinase(CK) and isoenzyme MB, and lactate dehydrogenase(LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

Serial values for hematologic and biochemical analysis after myocardial infarction in rats

Lee, Mi-Jin,Tae, Hyun-Jin,Li, Ying-Hua,Yu, Do-Hyeon,Han, In-Ae,Lee, Seok-Won,Ahn, Dong-Choon,Kim, In-Shik,Park, Jin-Ho The Korean Society of Veterinary Service 2008 韓國家畜衛生學會誌 Vol.31 No.2

To diagnose acute myocardial infarction (MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hematologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and biochemical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery (LMDA) of the left coronary artery was ligated during the progression (day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group (n=24, thoracotomy without LMDA ligation) and the experimental (MI) group (n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell (RBCs), hemoglobin and hematocrit (HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, cholesterol, total creatine kinase (CK) and isoenzyme MB, and lactate dehydrogenase (LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

GT rotary file을 이용한 근관성형법과 충전방법의 비교

강유미,진정희,유미경,이세준,이광원 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.5

The purpose of this study was to compare the shaping time of two shaping methods and the leakage of three different obturation techniques. Ninty three canaled human molar teeth were used. which were randomly divided into two groups of forty teeth each and ten control teeth. After working length determination. the one group was prepared crowndown technique using rotary root canal instruments of GT rotary files .12/20, .10/20, .08/20 and .06/20 taper(Maillefer Instrument SA. Switzerland). The other group was instrumented with Gates Gildden burs(#1, #2 and #3) to coronal preparation and GT rotary files .08/20 and .06/20 taper to apical preparation. Shaping time was measured. After root canals were instrumented, they were divided to three subgroups and obturated as follows: Subgroup 1, obturated with single cone method : Subgroup 2, obturated with lateral condensation : Subgroup 3, obturated with continuous wave technique. Three subgroups were obturated using non-standardized gutta-percha cone(Diadent, Korea, 06 or .08 taper) and AH-26(Dentsply DeTrey, Germany) as a root canal cement. Ten unobturated teeth served as positive and negative controls. After immersion in 2% methylene blue solution for 1 month, the teeth were washed during 24h. The teeth were determineralized in 10% nitric aciid and dehydrated by immersion in 80, 90 and 100% ethyl alcohol. The teeth were finally cleared and stored in 100% methylsalicylate, and apical dye penetration was evaluated under stereomicroscope(Leica M420, LC, U.S.A) at (×) 8.75 magnification. Liner measurement of dye penetration was assessed with the use of digitalized image analysing system (analySIS, GmbH, Germany), The data were analysed statistically using independent T-test and Two-way ANOVA and Tukey test. The result were as follows: 1. In canal prepared with GT^Trotary file, shaphing time taked more than the group of using Gates Gildden drill to coronal preparation without statistical significant (p>0.05). 2. The group of single cone obturation using canal preparation of GT^TM rotary files showed significantly more apical leakage than those of lateral condensation and continuous wave technique regardless of shaping method (p<0.05). 3. The group of single cone obturation using canal preparation of GT^TM rotary files and Gates Gildden drill showed significantly more apical leakage than those of continuous wave technique regardless of shaping method (p<0.05). 4. Regardless of shaping method. The group of continuous wave obturation showed less apical leakage than those of lateral condensation without statistical significance (p>0.05). 5. The group of single cone obturation using canal preparation of GT^TM rotary files and Gates Gildden drill showed more apical leakage than the group of lateral condensation using sane shaping method without statistical significance (p>0.05).

신규간호사와 프리셉터가 인지하는 프리셉터의 교수효율성 비교

김지양,김영선,김춘실,박현숙,신미영,윤연숙,조유숙,박미미,유문숙 한국간호과학회 간호행정학회 2009 간호행정학회지 Vol.15 No.2

Objective: The purpose of this study was to compare preceptors and new graduate nurses on their perception of preceptor teaching effectiveness. Methods: The participants were 90 new nurses and 90 preceptors who worked in A medical center. The data were collected from July 1 to September 30, 2008. A cross-sectional descriptive survey was done using a structured questionnaire. Results: New nurses' perception (4.07±.44) of the preceptor teaching effectiveness was significantly higher than perception of the preceptors (3.57±.37). Fifty five percent of new graduate nurses reported a lack of coherence in the preceptor's practice guidelines. For 5 causal factors classified on the teaching effectiveness, the factor of 'Professional knowledge and ability' showed the highest score, but 'Interpersonal and communication skill' got lowest score for both group. Preceptors responded that they did not have enough time to teach well because of their heavy workloads. Conclusions: These results suggest that the preceptors need appropriate compensation and education opportunities, and new graduate nurses need consistent education by the teaching professionals. Therefore, it is important to give preceptors full charge of the preceptorship. Also, it will be necessary to develop education programs to enhance interpersonal and communication skill for preceptors and new nurses.

개에서 이환된 장림프관 확장증 2례

송유미,이영원 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2011 動物醫科學硏究誌 Vol.18 No.1

A 2-year-old intact male Rottweiler (Case 1) presented with chronic diarrhea and weight loss. a 4-year-old intact female Yorkshire terrier (Case 2) had the history of chronic ascites. Common clinicopathologic findings were hypoalbuminemia, hypocalcemia and hypoglobuliemia. Abdominal radiographic finding were ascites. On abdominal ultrasonography, intestinal abnormalities including hyperechoic mucosal striation, thickened wall were detected. In case 1, exploratory laparotomy findings were distended lymphatic vessel and white nodular masses in serosa of intestine. On histopathologic examination, dilated lymphatic vessel in mucosa and inflammation in villi were founded. Based on these results, Case 1 was diagnosed definitely as intestinal lymphangiectasia. Based on ultasonographic findings, Case 2 tentatively diagnosed as intestinal lymphangiectasia and inflammatoy bowel disease. At 14 days after surgery, Case 1 was dead because of respiratory sign. At Case 2, ascites is presently being controlled by using prednisolone, antibiotics.

개에서 림프병증의 초음파학적 특징에 대한 증례

송유미,이영원 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2011 動物醫科學硏究誌 Vol.18 No.1

The objective of this report was to compare ultrasonographic features of lymphanderopathy of a case in our hospital to result of previous studies with the hope of identifying predictive parameter that differenciate lymphandenopathy. A 2-year-old male mongrel dog's chief complaints was dyspnea from three weeks ago. On physical examination. Swelling of submandibular and prescapular lymph node was founded. Radiography showed increased radioopacity of cranioventral lung field and widened cranial mediastinal lesion with soft tissue opacity on the thoracic views. And ill-defined mass with soft tissue opacity on the mid-abdomen views was detected. On ultrasonography, short/long axis ratio of prescapular lymph node is 0.74. In deep lymph nodes, short/long axis ratio was respectively 0.56, 0.43, 0.67, 0.61. Irregular contour and hyperechoic perinodal fat were common features. This features are coreespond with sonographic features of malignant lymph node of previous study. Based on cytologic results, lymphoma was diagnosed. Ultrasound provides noninvasive, real-time imaging information of both peripheral and more deeply lymph node.

녹차추출물에 의한 식중독 원인균인 장내세균 Plesiomonas shigelloides의 세포반응

유미옥,천재우,오계헌 순천향대학교 기초과학연구소 2002 순천향자연과학연구 논문집 Vol.8 No.2

The aim of this work was to investigate the cellular responses in the food-poisoning bacterium, Plesimonas shigelloids in response to tea polyphenol (TPP) extracted from green tea. The stress shock proteins, which contribute to the resistance of the cytotoxic effect of TPP, were induced at different TPP concentrations in exponentially growing culture of P. shigelloides. This response involved the induction of a 70-kDa DnaK and a 60-kDa GroEL monoclonal antibodies, characterized by SDS-PAGE and Western blot by the use of the anti-DnaK and anti-GroEL monoclonal antibodies. Survival of P. shigelloides with time in the presence of different concentrations of TPP was monitored, and viable counts paralleled the induction of the stress shock proteins in this strain. This strain killed in the concentration of 1,000 ㎍/㎖ TPP within 2 hours of incubation. Scanning electron microscopy for the cells treated with 10,000 ㎍/㎖ for 12 hours showed the presence of perforations and irregular rod shaped with wrinkled surfaces.

역행성 심정지 도관 삽입을 위한 술 중 경식도심초음파의 유용성

유미숙;최원주;김정원;박장수;김경태;김지연;어상일 인제대학교 2011 仁濟醫學 Vol.32 No.-

Background: Retrograde cardioplegia catheter (RCC) insertion is one of methods for administration of cardioplegics during cardiopulmonary bypass (CPB). However its success rate depends on only surgeon's experience. We thought the transesophageal echocardiography (TEE) can guide the R CC insertion, because TEE is commonly used for monitoring status of heart during cardiac surgery. This study was designed to evaluate the RC C insertion time by TEE guidance versus conventional method without T EE guidance. Methods: Sixty patients who were scheduled to undergo elective coronary artery bypass graft (CABG) surgery under general anesthesia were divided into groups as follows: group T [RCC insertion into coronary sinus (CS) by TEE guidance, n=30] and group C [RCC insertion into CS by conventional method, n=30]. We recorded the time of insertion of RCC in to CS in two groups. RCC insertion was done by TEE guidance in case that time was over two minutes by conventional methods in group C. Results: The overall time of RCC insertion into CS was 63.8 ± 23.6 seconds in the Group T and 102.3 ±55.7 seconds in the Group C, respectively (p=0.001). There were no complications during RCC into CS by T EE guidance. Conclusions: TEE guidance for RCC insertion into CS is shorten the time of insertion.

류마티스 관절염 환자에게 스테로이드 유발성 골다공증의 관리 상태에 대한 분석

유민아,이지수 이화여자대학교 의과대학 2005 EMJ (Ewha medical journal) Vol.28 No.1

Objectives : To examine the management status of patients with rheumatoid arthritis (RA) taking glucocorticoids treated at a tertiary hospital rheumatology Practice. Methods : A retrospective chart review was performed on 114 RA patients who were noted to be taking glucocorticoids during the period of 2000.7 to 2003.7, and who had been followed up for at least one year period. Use of bone densitometry and use of prescription medication for osteoporosis management were assessed. Results : Thirty one % of patients underwent bone densitometry, and 34% of patients received prescription medication for osteoporosis management. Sixty-six percent of patents who underwent bone densitometry showed osteoporosis with T score of less than 2.5, Osteoporosis medication was prescribed most often in the prednisolone exposure group of at least 7.5㎎/d for at least 6 months. Factors associated with not receiving management for osteoporosis included male sex, young age, and premenopausal woman. Conclusion : The use of bone densitometry and prescription medication for osteoporosis were suboptimal. Interventions to improve detection and prevention of glucocorticoid induced osteoporosis are necessary.

구치부 2급와동에 있어 심미성 수복물의 변연접합도에 관한 연구

유미경,이광원,손호현 全北大學校 齒醫學硏究所 1993 전북치대논문집 Vol.11 No.1

The purpose of this study was to evaluate the cervical marginal adaptation of direst composite resin, composite resin, composite resin inlay and porcelain inlay technique. 40 cavities of class Ⅱ were prepared 40 extracted maxillary premolr teeth, which were divided into four groups. Group 1 : restored by direct filling method(P50) Group 2 : restored by direct filling method(Z100) Group 3 : restored by resin inlay method(Estilux posterior CVS) Group 4 : restored by procelain inlay method(Vita-dur N) All the specimens were embedded into stainless steel mold after thermocycling between 5℃ and 55℃. Phototaking under microscopic image and measuring the marginal gap on the photographs and statistical analysis were peformed Specimens from each group were cut mesio-distally and observed under SEM. The results were as follows : 1. The groups restored by inlay method showed significantly less marginal gap than groups restored by direct filling method(P<0.05). 2. There was no significant difference in marginal gap between composite resin inlay group with porcelain inlay group(P>0.05). 3. The group restored by direct filling method(Z100) showed significantly less marginal gap than group restored by direct filling method(P5O)(P<0.05). 4. SEM examination indicated that direct composite resin groups were larger gaps(10- l5μm) than groups(2-5μm).